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The map label for this gene is folD

Identifier: 93005470

GI number: 93005470

Start: 758032

End: 758928

Strand: Direct

Name: folD

Synonym: Pcryo_0640

Alternate gene names: 93005470

Gene position: 758032-758928 (Clockwise)

Preceding gene: 93005469

Following gene: 93005471

Centisome position: 24.77

GC content: 46.6

Gene sequence:

>897_bases
ATGTCAATTGCGTCTATTACGTCAACTGCCCAAGGATCTGCTACCGTTTTGGACGGTAAAGCACTTGCTAAACAAATAGA
ACAGCAACTGCGCACGCGTGTAGACGCTATTAAAGCTAAGACAGGACGTACACCAAGCTTAGCGACGATATTGGTCGGTG
ACGATCCAGCTTCTGCGACTTATGTACGTATGAAAGGTAATGCTTGTAAGCGTGTCGGCATGGATTCTATTCGGGTTGAG
ATGCCAAGTGTAACGACGACTGCAGAGTTGATGGCAAAAATATACGAGCTGAATAGCAGTCCAGATGTTCATGGGATTTT
GCTGCAGCACCCAGTACCGGCTCATATTGATGAGCGTGCTTGCTTTGAGAAGATTGACTTGGCAAAAGACGTCGATGGTG
TGACCTGTCTTGGCTTTGGTCGTATGGCAATGCAGCAGTCCGCTTATGGCTCATGTACCCCGCAAGGTATCATGCATTTA
CTAGAGCAGCATAATATTGAGCTGGCTGGTTTGGAGGCCGTGGTAGTGGGTCGTAGTGCAATTTTAGGTAAACCAATGGC
AATGATGCTATTAAATGCCAACTGTACAGTAACAATCTGCCATTCAAGAACGCAAGATTTAGAGTCTCATATTAAACGTG
CTGATATCATAGTTGGAGCAGTAGGTGTACCTGAGCTGATCAAAGCTGATTGGATTAAAGCAGGCGCGGTAGTCATCGAT
GCAGGATTCCATCCGACTGATAATGGCGGCGTCGGTGATATCGAGCTGCAAGGTGTAGAAAATATTGCCAGTGCTTATAC
ACCAGTCCCAGGCGGCGTTGGGCCTATGACGATTAATACGCTTATTCGCCAGACCGTTGATGCTGCTGAAAAAGCTGCCG
GTTTAGACAATAGTTAA

Upstream 100 bases:

>100_bases
AATATGAGTATCAAACATACCTTGATCAAGGATAAGACAGGCATGCAAGCAATTGCGGCGCTGTCTTTACTTTTTTATCG
CACCCTCTATTGGAGTTACT

Downstream 100 bases:

>100_bases
TCATAAGAGCATGGATTGCCGATAGATAAGGAGCCTGAGACTTCACAGTAGACAGCACAAAGACTCAGGCTAAATCAACC
CCCTTACCGTTGATAGGATC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSIASITSTAQGSATVLDGKALAKQIEQQLRTRVDAIKAKTGRTPSLATILVGDDPASATYVRMKGNACKRVGMDSIRVE
MPSVTTTAELMAKIYELNSSPDVHGILLQHPVPAHIDERACFEKIDLAKDVDGVTCLGFGRMAMQQSAYGSCTPQGIMHL
LEQHNIELAGLEAVVVGRSAILGKPMAMMLLNANCTVTICHSRTQDLESHIKRADIIVGAVGVPELIKADWIKAGAVVID
AGFHPTDNGGVGDIELQGVENIASAYTPVPGGVGPMTINTLIRQTVDAAEKAAGLDNS

Sequences:

>Translated_298_residues
MSIASITSTAQGSATVLDGKALAKQIEQQLRTRVDAIKAKTGRTPSLATILVGDDPASATYVRMKGNACKRVGMDSIRVE
MPSVTTTAELMAKIYELNSSPDVHGILLQHPVPAHIDERACFEKIDLAKDVDGVTCLGFGRMAMQQSAYGSCTPQGIMHL
LEQHNIELAGLEAVVVGRSAILGKPMAMMLLNANCTVTICHSRTQDLESHIKRADIIVGAVGVPELIKADWIKAGAVVID
AGFHPTDNGGVGDIELQGVENIASAYTPVPGGVGPMTINTLIRQTVDAAEKAAGLDNS
>Mature_297_residues
SIASITSTAQGSATVLDGKALAKQIEQQLRTRVDAIKAKTGRTPSLATILVGDDPASATYVRMKGNACKRVGMDSIRVEM
PSVTTTAELMAKIYELNSSPDVHGILLQHPVPAHIDERACFEKIDLAKDVDGVTCLGFGRMAMQQSAYGSCTPQGIMHLL
EQHNIELAGLEAVVVGRSAILGKPMAMMLLNANCTVTICHSRTQDLESHIKRADIIVGAVGVPELIKADWIKAGAVVIDA
GFHPTDNGGVGDIELQGVENIASAYTPVPGGVGPMTINTLIRQTVDAAEKAAGLDNS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=38.6206896551724, Blast_Score=204, Evalue=7e-53,
Organism=Homo sapiens, GI94721354, Length=302, Percent_Identity=38.7417218543046, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI222418558, Length=301, Percent_Identity=37.8737541528239, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI36796743, Length=210, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=45.1263537906137, Blast_Score=237, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17568735, Length=257, Percent_Identity=38.5214007782101, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=41.2587412587413, Blast_Score=226, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321643, Length=294, Percent_Identity=38.7755102040816, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=42.5170068027211, Blast_Score=214, Evalue=4e-56,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=42.5170068027211, Blast_Score=214, Evalue=4e-56,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=40.1384083044983, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=40.1384083044983, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=40.1384083044983, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=40.1384083044983, Blast_Score=206, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_PSYCK (Q1QD30)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579907.1
- ProteinModelPortal:   Q1QD30
- SMR:   Q1QD30
- STRING:   Q1QD30
- GeneID:   4035477
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0640
- NMPDR:   fig|335284.3.peg.3
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   GPMTINT
- ProtClustDB:   PRK14192
- BioCyc:   PCRY335284:PCRYO_0640-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31393; Mature: 31261

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIASITSTAQGSATVLDGKALAKQIEQQLRTRVDAIKAKTGRTPSLATILVGDDPASAT
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEE
YVRMKGNACKRVGMDSIRVEMPSVTTTAELMAKIYELNSSPDVHGILLQHPVPAHIDERA
EEEECCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHH
CFEKIDLAKDVDGVTCLGFGRMAMQQSAYGSCTPQGIMHLLEQHNIELAGLEAVVVGRSA
HHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCEEEEECCHH
ILGKPMAMMLLNANCTVTICHSRTQDLESHIKRADIIVGAVGVPELIKADWIKAGAVVID
HHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCEEEEE
AGFHPTDNGGVGDIELQGVENIASAYTPVPGGVGPMTINTLIRQTVDAAEKAAGLDNS
CCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SIASITSTAQGSATVLDGKALAKQIEQQLRTRVDAIKAKTGRTPSLATILVGDDPASAT
CCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEE
YVRMKGNACKRVGMDSIRVEMPSVTTTAELMAKIYELNSSPDVHGILLQHPVPAHIDERA
EEEECCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHH
CFEKIDLAKDVDGVTCLGFGRMAMQQSAYGSCTPQGIMHLLEQHNIELAGLEAVVVGRSA
HHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEECCEEEEECCHH
ILGKPMAMMLLNANCTVTICHSRTQDLESHIKRADIIVGAVGVPELIKADWIKAGAVVID
HHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHCCCEEEEE
AGFHPTDNGGVGDIELQGVENIASAYTPVPGGVGPMTINTLIRQTVDAAEKAAGLDNS
CCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA