Click here to switch to the map view.

The map label for this gene is rfbB1 [H]

Identifier: 93005454

GI number: 93005454

Start: 743285

End: 744382

Strand: Direct

Name: rfbB1 [H]

Synonym: Pcryo_0624

Alternate gene names: 93005454

Gene position: 743285-744382 (Clockwise)

Preceding gene: 93005453

Following gene: 93005455

Centisome position: 24.29

GC content: 39.98

Gene sequence:

>1098_bases
ATGAAATTTCTGATAACGGGCGGTGCGGGTTTCATTGGTTCTGCTGTGGTAAGGCATTTGCTACAGCATACACAAAACTC
ACTTATGAATCTTGATAAACTTACTTATGCAGGTAATTTAGAGTCATGTGATCCAGTGAGTGATAATTCACGCTATACAT
TTGAACAAGTTGATATTTGCAATCCTACTGAATTGGCTAGAGTCTTTGAAAGGTATCAGCCGGATGTGGTAATGCACTTA
GCTGCAGAAAGCCATGTTGATCGCTCTATCGATGCACCTGATGATTTTATACGAACCAATATTGTTGGTACTTATAATAT
GTTAGAGGCAGCGCGTGCTTACTGGAGTCACTTAAGTTCTGAGCGTAAACAAATCTTTCGTTTTCATCATATAAGCACTG
ACGAGGTTTATGGTGACCTACCTCATCCCGATAAGCTTGAAAACCCTAATTCAGTAGGTGAATACCTATTTACTGAAACA
ACCTCTTATGCGCCTAGTTCTCCTTACTCTGCTAGTAAAGCAAGCTCTGATCATCTAGTGCGTGCTTGGTATAGAACTTA
TGGTCTACCTGTAGTTATAACAAACTGTTCTAATAATTATGGACCTTATCATTTTCCTGAAAAATTAGTTCCGCATATTA
TTTTAAACGCATTGGCTGGTAAGTCTTTACCGGTCTATGGTGACGGCAGCCAGATCCGTGATTGGCTATATGTAGAAGAT
CATGCCCGCGCTTTAATTAAAGTAGCAACTGAAGGCGTGGTTGGTGAGACTTATAACATCGGCGGTCATAATGAAAAACG
CAATATTGATGTAGTACTTATACTATGTGCGTTGCTTGAGGAGATGGCTTCTGATCAGAAGCCCGCGGGTATAGCTAAGT
ACGAAGATTTAATTGTATTCGTCAAAGATCGTGCAGGTCATGATGTTCGTTACGCTATAGATGCTAGTAAAATCGAACGT
GAATTAGACTGGGTTCCAGAAGAAAGCTTTGAAACCGGTATGCGTAAAACAGTGCAATGGTATTTAGATAATAAAAATTG
GTGGCAGCGTGTGCTAAATGGAAGTTACCGCTTAGAACGATTGGGAGAGTATAAATGA

Upstream 100 bases:

>100_bases
GATATAGTTGAAGAGTTTTCTAGGAAGATTCAGGCAGAGAACGTGCTTGCTTTTCTGGATAAAGCTTAATTCAAAAATAA
ATAATAAGGCTCAAGTTTAT

Downstream 100 bases:

>100_bases
AAGGTATTATTTTAGCTGGCGGTTCAGGTACACGCCTACATCCTATTACTCGTGGTGTGTCTAAGCAGTTACTACCAATT
TATGATAAGCCGATGATTTA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKFLITGGAGFIGSAVVRHLLQHTQNSLMNLDKLTYAGNLESCDPVSDNSRYTFEQVDICNPTELARVFERYQPDVVMHL
AAESHVDRSIDAPDDFIRTNIVGTYNMLEAARAYWSHLSSERKQIFRFHHISTDEVYGDLPHPDKLENPNSVGEYLFTET
TSYAPSSPYSASKASSDHLVRAWYRTYGLPVVITNCSNNYGPYHFPEKLVPHIILNALAGKSLPVYGDGSQIRDWLYVED
HARALIKVATEGVVGETYNIGGHNEKRNIDVVLILCALLEEMASDQKPAGIAKYEDLIVFVKDRAGHDVRYAIDASKIER
ELDWVPEESFETGMRKTVQWYLDNKNWWQRVLNGSYRLERLGEYK

Sequences:

>Translated_365_residues
MKFLITGGAGFIGSAVVRHLLQHTQNSLMNLDKLTYAGNLESCDPVSDNSRYTFEQVDICNPTELARVFERYQPDVVMHL
AAESHVDRSIDAPDDFIRTNIVGTYNMLEAARAYWSHLSSERKQIFRFHHISTDEVYGDLPHPDKLENPNSVGEYLFTET
TSYAPSSPYSASKASSDHLVRAWYRTYGLPVVITNCSNNYGPYHFPEKLVPHIILNALAGKSLPVYGDGSQIRDWLYVED
HARALIKVATEGVVGETYNIGGHNEKRNIDVVLILCALLEEMASDQKPAGIAKYEDLIVFVKDRAGHDVRYAIDASKIER
ELDWVPEESFETGMRKTVQWYLDNKNWWQRVLNGSYRLERLGEYK
>Mature_365_residues
MKFLITGGAGFIGSAVVRHLLQHTQNSLMNLDKLTYAGNLESCDPVSDNSRYTFEQVDICNPTELARVFERYQPDVVMHL
AAESHVDRSIDAPDDFIRTNIVGTYNMLEAARAYWSHLSSERKQIFRFHHISTDEVYGDLPHPDKLENPNSVGEYLFTET
TSYAPSSPYSASKASSDHLVRAWYRTYGLPVVITNCSNNYGPYHFPEKLVPHIILNALAGKSLPVYGDGSQIRDWLYVED
HARALIKVATEGVVGETYNIGGHNEKRNIDVVLILCALLEEMASDQKPAGIAKYEDLIVFVKDRAGHDVRYAIDASKIER
ELDWVPEESFETGMRKTVQWYLDNKNWWQRVLNGSYRLERLGEYK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=351, Percent_Identity=33.048433048433, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI42516563, Length=343, Percent_Identity=25.0728862973761, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI56237023, Length=288, Percent_Identity=26.7361111111111, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI56118217, Length=288, Percent_Identity=26.7361111111111, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI189083684, Length=288, Percent_Identity=26.7361111111111, Blast_Score=72, Evalue=9e-13,
Organism=Escherichia coli, GI48994969, Length=361, Percent_Identity=70.3601108033241, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=67.7871148459384, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1786974, Length=366, Percent_Identity=27.0491803278689, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788366, Length=249, Percent_Identity=27.3092369477912, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788365, Length=360, Percent_Identity=23.6111111111111, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=346, Percent_Identity=33.8150289017341, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI115532424, Length=338, Percent_Identity=32.5443786982249, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17539532, Length=342, Percent_Identity=24.5614035087719, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=381, Percent_Identity=24.9343832020997, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=383, Percent_Identity=24.8041775456919, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=379, Percent_Identity=25.8575197889182, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356223, Length=345, Percent_Identity=24.9275362318841, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19923002, Length=378, Percent_Identity=25.1322751322751, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41558; Mature: 41558

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLITGGAGFIGSAVVRHLLQHTQNSLMNLDKLTYAGNLESCDPVSDNSRYTFEQVDIC
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCC
NPTELARVFERYQPDVVMHLAAESHVDRSIDAPDDFIRTNIVGTYNMLEAARAYWSHLSS
CHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
ERKQIFRFHHISTDEVYGDLPHPDKLENPNSVGEYLFTETTSYAPSSPYSASKASSDHLV
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
RAWYRTYGLPVVITNCSNNYGPYHFPEKLVPHIILNALAGKSLPVYGDGSQIRDWLYVED
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHEEEEHH
HARALIKVATEGVVGETYNIGGHNEKRNIDVVLILCALLEEMASDQKPAGIAKYEDLIVF
HHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
VKDRAGHDVRYAIDASKIERELDWVPEESFETGMRKTVQWYLDNKNWWQRVLNGSYRLER
EECCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHEEECCHHHHHHHHCCCHHHHH
LGEYK
HCCCC
>Mature Secondary Structure
MKFLITGGAGFIGSAVVRHLLQHTQNSLMNLDKLTYAGNLESCDPVSDNSRYTFEQVDIC
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEECCCC
NPTELARVFERYQPDVVMHLAAESHVDRSIDAPDDFIRTNIVGTYNMLEAARAYWSHLSS
CHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
ERKQIFRFHHISTDEVYGDLPHPDKLENPNSVGEYLFTETTSYAPSSPYSASKASSDHLV
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
RAWYRTYGLPVVITNCSNNYGPYHFPEKLVPHIILNALAGKSLPVYGDGSQIRDWLYVED
HHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHEEEEHH
HARALIKVATEGVVGETYNIGGHNEKRNIDVVLILCALLEEMASDQKPAGIAKYEDLIVF
HHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
VKDRAGHDVRYAIDASKIERELDWVPEESFETGMRKTVQWYLDNKNWWQRVLNGSYRLER
EECCCCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHHEEECCHHHHHHHHCCCHHHHH
LGEYK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]