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The map label for this gene is asnB [H]

Identifier: 93005452

GI number: 93005452

Start: 739852

End: 741744

Strand: Direct

Name: asnB [H]

Synonym: Pcryo_0622

Alternate gene names: 93005452

Gene position: 739852-741744 (Clockwise)

Preceding gene: 93005451

Following gene: 93005453

Centisome position: 24.18

GC content: 35.92

Gene sequence:

>1893_bases
TTGTGTGGATTTTTAGGATTTATTTCAGAAAGAAACTATGAATCACTTTTAGTTGATTCTCTTGATGTCATTAAGCATCG
TGGACCAGATAAACAGTTATCTCTCGTACAGCGATCTGAAAGTTATTATGTTGGTTTAGGTCATGCTCGTCTTTCAATTA
TTGATTTAAGTGATGAAGCTTCTCAACCATTTATATCAAAATGTGGTCGTTATAACCTCGTTTTCAATGGTGAGATTTAT
AACTATAAAGAATTACGAGTTGAACTATTAGCACTAGGCTTTGATTTTACTACTGACTCTGATACAGAGGTACTTTTAAA
TGCTTGGATAGCTTGGGGCTTAGAAGCTTTACCCAAGTTTATTGGAATGTTTTCATTTGTTATATTTGACAAGGCTGCTA
AAAAAATAGTTCTAGTTAGAGATGCTTTTGGAATTAAGCCTTTTTTTTATACTTTGCCCCAAGATAAAAGAGTTTTCTTT
GGTTCAGACATACGTGGATTAATAAAACTTCGTAATACTACTTTCGAACCTAATTTGCAAAGAGCTTATGATTATTTAGT
ACATGGTGACTATGACTCTACTGAGTCTACCTTTGTTGAAGGAGTCAAACATATAATGCCAGGGCATTATTTAGAATTTA
GTTTGATTACTGGGAATATGACTGAGCCTAAAGCTTGGTGGCAGCCCGATCTAAGCTATACTTCTACGCTTAGTTTTGAT
GAGGCAAAAGATAAATTGCGTACCCTATTTCTAGATAGTGTAAAACTGCATTTACGTAGTGATGTTCCATTGGGCGTTGC
TCTTTCAGGTGGTGTAGATTCTTCAGCCGTTGTATGTGCAGTTCGTCATCTTGAACCAAACACGCCAATTCATACATTTA
GTTATATTGCATCTGACAATCGTATCTCTGAGGAAGTATGGGTAGACCTAATTAATAAAGATATTAAGGCGATAGAACAT
AAAGTGGTTTCAAACTCAGCAGAAATGCAAAAAGATTTAGATAATATGTTGCTGATGCAGGGTGAGCCTTTTGGTGGTAC
AAGTATCTATGCACAATATCGCGTATTTAAATTAGCAAAAGAAAGTGGTATTACAGTCACCTTAGATGGTCAAGGTGCAG
ACGAGTTGCTAGCTGGTTACAACGGCTATCCAGGTAGAAGGCTACTAAGTATTTTAGAAACTGGTAATTTTATCAAAGCA
AAATATTTTGCGAAACACTGGGCTACCTTTCCTGGGAGAAGTTATCTTACTCCATGGAAAAATCTTGCCAGCTTAAAGCT
TCCAAATTTATTATATAAAGTAGCTAAAAAAACTACTGGTCGGGACAGCTATACATCAGGATTGAATATTGATTATTTAA
TTAATAAGGGAGTTAATTTTGAAGTTGATCGTTCAATTCTTTCAAAGAAAAATAAAGGAAAGCGTGTTAGAGAAGCTTTA
GCTGAATCTATAGAAAGCAAAGGTCTACCATCGTTGTTGCGCCATGGTGATCGTAATTCAATGGCGTTCTCGATTGAAAG
CCGTGTTCCTTTCTTAACGTTGCCAATGGCTGAGTTTTTATTAAGCTTGCCTGAAAATTATTTAATCTCTAATAAAGGTG
TAACGAAGCATATTTTTCGTGAAGCTATGCGTGGCATTGTTCCTGATAGCCATTTAGACAGAAAGGATAAGATTGGTTTT
GAAACTCCAGAAGCAGAATGGCTATTAAGTATGAGTGGTGCTATAGAAACTTGGATAAATGATGCACCAGAAATCCCTTT
TATTAATAAAGCAGAAGTTTTAGAGGGGTTTAAGCAGGTCATACTTGGAGAGCGTAGCTTTGATAGCAGAGTGTGGCGAT
GGGTTAATTACATACGGTGGTACACCCTAATGGAAATAGCAACGTTAAATTAA

Upstream 100 bases:

>100_bases
TTTTTGTATTCTTTTATTTTTATTAGCTGGCTTGATATCGTTAAAAGACTTTAAACAATTTCGAGATTTATTAAAGAAAT
AAATCTGAGGAGAATTTAAT

Downstream 100 bases:

>100_bases
TAATGCTTAATTTATTAATAAACTAGGTTTTAAATTTTAGGATTAATTCTAAAATATGATTTAAATCTAACTAACTTGAA
GTGTTAGTGCTGTATATCAC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MCGFLGFISERNYESLLVDSLDVIKHRGPDKQLSLVQRSESYYVGLGHARLSIIDLSDEASQPFISKCGRYNLVFNGEIY
NYKELRVELLALGFDFTTDSDTEVLLNAWIAWGLEALPKFIGMFSFVIFDKAAKKIVLVRDAFGIKPFFYTLPQDKRVFF
GSDIRGLIKLRNTTFEPNLQRAYDYLVHGDYDSTESTFVEGVKHIMPGHYLEFSLITGNMTEPKAWWQPDLSYTSTLSFD
EAKDKLRTLFLDSVKLHLRSDVPLGVALSGGVDSSAVVCAVRHLEPNTPIHTFSYIASDNRISEEVWVDLINKDIKAIEH
KVVSNSAEMQKDLDNMLLMQGEPFGGTSIYAQYRVFKLAKESGITVTLDGQGADELLAGYNGYPGRRLLSILETGNFIKA
KYFAKHWATFPGRSYLTPWKNLASLKLPNLLYKVAKKTTGRDSYTSGLNIDYLINKGVNFEVDRSILSKKNKGKRVREAL
AESIESKGLPSLLRHGDRNSMAFSIESRVPFLTLPMAEFLLSLPENYLISNKGVTKHIFREAMRGIVPDSHLDRKDKIGF
ETPEAEWLLSMSGAIETWINDAPEIPFINKAEVLEGFKQVILGERSFDSRVWRWVNYIRWYTLMEIATLN

Sequences:

>Translated_630_residues
MCGFLGFISERNYESLLVDSLDVIKHRGPDKQLSLVQRSESYYVGLGHARLSIIDLSDEASQPFISKCGRYNLVFNGEIY
NYKELRVELLALGFDFTTDSDTEVLLNAWIAWGLEALPKFIGMFSFVIFDKAAKKIVLVRDAFGIKPFFYTLPQDKRVFF
GSDIRGLIKLRNTTFEPNLQRAYDYLVHGDYDSTESTFVEGVKHIMPGHYLEFSLITGNMTEPKAWWQPDLSYTSTLSFD
EAKDKLRTLFLDSVKLHLRSDVPLGVALSGGVDSSAVVCAVRHLEPNTPIHTFSYIASDNRISEEVWVDLINKDIKAIEH
KVVSNSAEMQKDLDNMLLMQGEPFGGTSIYAQYRVFKLAKESGITVTLDGQGADELLAGYNGYPGRRLLSILETGNFIKA
KYFAKHWATFPGRSYLTPWKNLASLKLPNLLYKVAKKTTGRDSYTSGLNIDYLINKGVNFEVDRSILSKKNKGKRVREAL
AESIESKGLPSLLRHGDRNSMAFSIESRVPFLTLPMAEFLLSLPENYLISNKGVTKHIFREAMRGIVPDSHLDRKDKIGF
ETPEAEWLLSMSGAIETWINDAPEIPFINKAEVLEGFKQVILGERSFDSRVWRWVNYIRWYTLMEIATLN
>Mature_630_residues
MCGFLGFISERNYESLLVDSLDVIKHRGPDKQLSLVQRSESYYVGLGHARLSIIDLSDEASQPFISKCGRYNLVFNGEIY
NYKELRVELLALGFDFTTDSDTEVLLNAWIAWGLEALPKFIGMFSFVIFDKAAKKIVLVRDAFGIKPFFYTLPQDKRVFF
GSDIRGLIKLRNTTFEPNLQRAYDYLVHGDYDSTESTFVEGVKHIMPGHYLEFSLITGNMTEPKAWWQPDLSYTSTLSFD
EAKDKLRTLFLDSVKLHLRSDVPLGVALSGGVDSSAVVCAVRHLEPNTPIHTFSYIASDNRISEEVWVDLINKDIKAIEH
KVVSNSAEMQKDLDNMLLMQGEPFGGTSIYAQYRVFKLAKESGITVTLDGQGADELLAGYNGYPGRRLLSILETGNFIKA
KYFAKHWATFPGRSYLTPWKNLASLKLPNLLYKVAKKTTGRDSYTSGLNIDYLINKGVNFEVDRSILSKKNKGKRVREAL
AESIESKGLPSLLRHGDRNSMAFSIESRVPFLTLPMAEFLLSLPENYLISNKGVTKHIFREAMRGIVPDSHLDRKDKIGF
ETPEAEWLLSMSGAIETWINDAPEIPFINKAEVLEGFKQVILGERSFDSRVWRWVNYIRWYTLMEIATLN

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=394, Percent_Identity=25.8883248730964, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI168229252, Length=394, Percent_Identity=25.8883248730964, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI168229250, Length=394, Percent_Identity=25.8883248730964, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI296010848, Length=371, Percent_Identity=26.1455525606469, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI296010852, Length=301, Percent_Identity=25.9136212624585, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI296010850, Length=301, Percent_Identity=25.9136212624585, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786889, Length=378, Percent_Identity=27.5132275132275, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325403, Length=421, Percent_Identity=26.1282660332542, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321563, Length=420, Percent_Identity=25.952380952381, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45553209, Length=370, Percent_Identity=29.7297297297297, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71497; Mature: 71497

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGFLGFISERNYESLLVDSLDVIKHRGPDKQLSLVQRSESYYVGLGHARLSIIDLSDEA
CCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCC
SQPFISKCGRYNLVFNGEIYNYKELRVELLALGFDFTTDSDTEVLLNAWIAWGLEALPKF
CCCHHHHCCCEEEEECCCCCCHHHHEEEHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
IGMFSFVIFDKAAKKIVLVRDAFGIKPFFYTLPQDKRVFFGSDIRGLIKLRNTTFEPNLQ
HHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCEEEECCCCHHHHEECCCCCCCCHH
RAYDYLVHGDYDSTESTFVEGVKHIMPGHYLEFSLITGNMTEPKAWWQPDLSYTSTLSFD
HHHHHHEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCEECCHH
EAKDKLRTLFLDSVKLHLRSDVPLGVALSGGVDSSAVVCAVRHLEPNTPIHTFSYIASDN
HHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHEEEEECCCCCCCCEEEEEECCCC
RISEEVWVDLINKDIKAIEHKVVSNSAEMQKDLDNMLLMQGEPFGGTSIYAQYRVFKLAK
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
ESGITVTLDGQGADELLAGYNGYPGRRLLSILETGNFIKAKYFAKHWATFPGRSYLTPWK
HCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCHH
NLASLKLPNLLYKVAKKTTGRDSYTSGLNIDYLINKGVNFEVDRSILSKKNKGKRVREAL
HHHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEECHHHHCCCCCCHHHHHHH
AESIESKGLPSLLRHGDRNSMAFSIESRVPFLTLPMAEFLLSLPENYLISNKGVTKHIFR
HHHHHHCCCHHHHHCCCCCCEEEEECCCCCEEECCHHHHHHHCCCCCEECCCCHHHHHHH
EAMRGIVPDSHLDRKDKIGFETPEAEWLLSMSGAIETWINDAPEIPFINKAEVLEGFKQV
HHHHCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ILGERSFDSRVWRWVNYIRWYTLMEIATLN
HCCCCCHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MCGFLGFISERNYESLLVDSLDVIKHRGPDKQLSLVQRSESYYVGLGHARLSIIDLSDEA
CCCCHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCCEEEEEECCCCC
SQPFISKCGRYNLVFNGEIYNYKELRVELLALGFDFTTDSDTEVLLNAWIAWGLEALPKF
CCCHHHHCCCEEEEECCCCCCHHHHEEEHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
IGMFSFVIFDKAAKKIVLVRDAFGIKPFFYTLPQDKRVFFGSDIRGLIKLRNTTFEPNLQ
HHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCEEEECCCCHHHHEECCCCCCCCHH
RAYDYLVHGDYDSTESTFVEGVKHIMPGHYLEFSLITGNMTEPKAWWQPDLSYTSTLSFD
HHHHHHEECCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCEECCHH
EAKDKLRTLFLDSVKLHLRSDVPLGVALSGGVDSSAVVCAVRHLEPNTPIHTFSYIASDN
HHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHEEEEECCCCCCCCEEEEEECCCC
RISEEVWVDLINKDIKAIEHKVVSNSAEMQKDLDNMLLMQGEPFGGTSIYAQYRVFKLAK
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHH
ESGITVTLDGQGADELLAGYNGYPGRRLLSILETGNFIKAKYFAKHWATFPGRSYLTPWK
HCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCHH
NLASLKLPNLLYKVAKKTTGRDSYTSGLNIDYLINKGVNFEVDRSILSKKNKGKRVREAL
HHHHHCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEECHHHHCCCCCCHHHHHHH
AESIESKGLPSLLRHGDRNSMAFSIESRVPFLTLPMAEFLLSLPENYLISNKGVTKHIFR
HHHHHHCCCHHHHHCCCCCCEEEEECCCCCEEECCHHHHHHHCCCCCEECCCCHHHHHHH
EAMRGIVPDSHLDRKDKIGFETPEAEWLLSMSGAIETWINDAPEIPFINKAEVLEGFKQV
HHHHCCCCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ILGERSFDSRVWRWVNYIRWYTLMEIATLN
HCCCCCHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]