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The map label for this gene is oprF [H]

Identifier: 93005426

GI number: 93005426

Start: 710751

End: 711407

Strand: Direct

Name: oprF [H]

Synonym: Pcryo_0596

Alternate gene names: 93005426

Gene position: 710751-711407 (Clockwise)

Preceding gene: 93005425

Following gene: 93005427

Centisome position: 23.23

GC content: 40.79

Gene sequence:

>657_bases
ATGAAAGCAATTTTATATTTAGTCAGCATTACTCTTTGTAGTTCTTTGGCATTATCAGCGTGTCAAACCGCGCCAAAACT
AGCGATTGATAACATAGTGACAGCGCCAATGCCAATATTAATGGTGAGCCTCGATTCTGATGGTGATGGATATTCTGATG
AGATAGACCTGTGTCCAGGCACCCCAAGAAATATAGTAACAGATGAAAGGGGTTGTCCTTTTACGGGTATGGGGATAGGC
CTAAAAATGGAATATCGTGCCTTTTTTGCCAAGGGCAGCAGTGAGTTATCAAAAGAGTATCAGCTTGAGCTTGATACAGT
AGCCCTAAAGCTGCAAGAGTACTATACGGCAACTATGCGTATTGAGGGACACGCTTCAGTAGATGAAATGTCAGATACAA
AATTGCGACCAAATTCTCTAGCAAGAAATAGGGCACTTATCGTCAAAAACTATCTTGTACTAAAGCATAAAATTGATCCT
GAGCGTTTAAATACCTTTAATTATGATGCCGAACAGCCTATTACCTCTTCTGATACAGAAGAAGGTAAGTCTATGAACCG
CAGAGTTTATGGGGTAGCTACTGCAGATTTTGATAGCGAAGAGGAAATGTCCATGAACCGCCGAATTTTCGAGATAGCTG
TAGAGCCTGAAGATTAA

Upstream 100 bases:

>100_bases
CAGCGTATTTATGCGCTTATTAGCAATGTTGAATAATATATTAAGCGTTTCAAAAAGATTGAAAATAAAGAATATGTGAT
AATTGAAAAAGGACGTTGAT

Downstream 100 bases:

>100_bases
TTGTTTTTAAACGTTTATGCCCTTACTAGCAATATTGAGTGTTTTAAAAGAGTGAAAAGTGATGACAGACAAAGATTTAA
ATTATTCTGGATAAAGAGAG

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKAILYLVSITLCSSLALSACQTAPKLAIDNIVTAPMPILMVSLDSDGDGYSDEIDLCPGTPRNIVTDERGCPFTGMGIG
LKMEYRAFFAKGSSELSKEYQLELDTVALKLQEYYTATMRIEGHASVDEMSDTKLRPNSLARNRALIVKNYLVLKHKIDP
ERLNTFNYDAEQPITSSDTEEGKSMNRRVYGVATADFDSEEEMSMNRRIFEIAVEPED

Sequences:

>Translated_218_residues
MKAILYLVSITLCSSLALSACQTAPKLAIDNIVTAPMPILMVSLDSDGDGYSDEIDLCPGTPRNIVTDERGCPFTGMGIG
LKMEYRAFFAKGSSELSKEYQLELDTVALKLQEYYTATMRIEGHASVDEMSDTKLRPNSLARNRALIVKNYLVLKHKIDP
ERLNTFNYDAEQPITSSDTEEGKSMNRRVYGVATADFDSEEEMSMNRRIFEIAVEPED
>Mature_218_residues
MKAILYLVSITLCSSLALSACQTAPKLAIDNIVTAPMPILMVSLDSDGDGYSDEIDLCPGTPRNIVTDERGCPFTGMGIG
LKMEYRAFFAKGSSELSKEYQLELDTVALKLQEYYTATMRIEGHASVDEMSDTKLRPNSLARNRALIVKNYLVLKHKIDP
ERLNTFNYDAEQPITSSDTEEGKSMNRRVYGVATADFDSEEEMSMNRRIFEIAVEPED

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 24328; Mature: 24328

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAILYLVSITLCSSLALSACQTAPKLAIDNIVTAPMPILMVSLDSDGDGYSDEIDLCPG
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCEECCCC
TPRNIVTDERGCPFTGMGIGLKMEYRAFFAKGSSELSKEYQLELDTVALKLQEYYTATMR
CCCCCCCCCCCCCEECCCCCEEEHHHHEECCCCHHHHHHHEEEHHHHHHHHHHHEEEEEE
IEGHASVDEMSDTKLRPNSLARNRALIVKNYLVLKHKIDPERLNTFNYDAEQPITSSDTE
EECCCCCCCCCCCCCCCCHHHCCCEEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCCCCH
EGKSMNRRVYGVATADFDSEEEMSMNRRIFEIAVEPED
HHHHHCCEEEEEEECCCCCHHHHHHCCEEEEEEECCCC
>Mature Secondary Structure
MKAILYLVSITLCSSLALSACQTAPKLAIDNIVTAPMPILMVSLDSDGDGYSDEIDLCPG
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCEECCCC
TPRNIVTDERGCPFTGMGIGLKMEYRAFFAKGSSELSKEYQLELDTVALKLQEYYTATMR
CCCCCCCCCCCCCEECCCCCEEEHHHHEECCCCHHHHHHHEEEHHHHHHHHHHHEEEEEE
IEGHASVDEMSDTKLRPNSLARNRALIVKNYLVLKHKIDPERLNTFNYDAEQPITSSDTE
EECCCCCCCCCCCCCCCCHHHCCCEEEEEEEEEEEECCCHHHHCCCCCCCCCCCCCCCCH
EGKSMNRRVYGVATADFDSEEEMSMNRRIFEIAVEPED
HHHHHCCEEEEEEECCCCCHHHHHHCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]