Click here to switch to the map view.

The map label for this gene is nuoM [H]

Identifier: 93005414

GI number: 93005414

Start: 696964

End: 698589

Strand: Direct

Name: nuoM [H]

Synonym: Pcryo_0584

Alternate gene names: 93005414

Gene position: 696964-698589 (Clockwise)

Preceding gene: 93005413

Following gene: 93005415

Centisome position: 22.78

GC content: 45.02

Gene sequence:

>1626_bases
ATGATTGAGTTACAACAAACGTGGATGCTACCAGCATTAATCGCCATTCCTTTTATTGCAGGATTGCTATGCTGGCTGGT
TGAACGCTTTAATAAACGCTTGCCGCGCTGGATTGCCTTAATCGGTATGACGTTGACCTTTGTATTGTCAATAGTGCTGT
GGCAATACGGTGATTTTTCTGGCATGAGTCAGCAAGTTATTGCGCCAGATGCTGCTGTGCCTTGGGTGGCTGAATTTAGT
GTGCCTTGGATACCGAGTTTTGGTATCAGCTTTCACTTAGCGTTAGATGGTTTATCGCTAATGATGGTTGCATTAACTGG
TCTGCTCGGTGTTGCCGCAGTTGCCTGTTCGTGGAATGAGATTCAGCGCCGTGTTGGTTTCTTCCATCTAAACTTGCTGT
GGAGTTTGGGCGGCGTCATCGGTGTTTTCTTAGCCATTGATATGTTCTTGTTCTTCTTCTTTTGGGAGATGATGCTGGTT
CCAATCTACTTTTTGATCGCGATTTGGGGTCATGATGTGGTTGGTAAAACCAAAGAATACGCAGCAACTAAGTTCTTTAT
TTATACCCAAGCATCTGGTCTTATCATGCTGGTCGGTATTTTGATATTGGTCATTATCAGCTACGCCCAAAAAGGCGTGG
TCAGCTTTAACTATAATGATTTACTCGGTACGTCACTTGGCGGCTGGGAATATCCGATCATGCTGTGCTTCTTTATTGGC
TTTGCCGTTAAGCTGCCAATTATTCCATTCCATGGCTGGTTACCTGATGCCCATGCGCAAGCACCAACGGCAGGTTCGGT
GGATTTGGCAGGGGTTCTAATTAAGACGGCGGCTTATGGTTTGATTCGTTTTGTCTTGCCATTATTTCCAGCGGCGTCAC
AAGATTTTGCGCCAATTGCTATGACCTTGGGTACTATTGGTATCTTCTATGGCGCATGGCTTGCCTTTATGCAAACCGAT
ATGAAGCGTCTGCTTGCTTATACCAGTATCTCGCATATGGGCTTTGTGGTTTTGGCAATTTATGCCGGAACCTTACTCAG
CTTGCAAGGTTTGATGATTCAAATGCTGGCACATGGTCTAAGCTCAGCAGCGCTGTTTATTATGGCAGGGCAGTTATATG
AGCGCTTACATACGCGCGATTTGACCTTGATGGGCGGTATGTGGGGTCAGTTCCGTTATTATGCGCCGATACTGATGTTC
TTCTGTGCGGCCTTACTTGGTATTCCGGGTACGGGTAACTTTATCGGTGAATTCTTGATCTTATTTGGTGCCTTTGCTCA
GTATCCTGTGTTTGTGGTCTTAGCAACTTTTAGCTTAGTATTGGCGGGTTTATACTCACTTATATTAATTCATAAAGCGC
TATTTGGTATCAACAACATTGAAGAAGTGGCATTAAAAGAAACCAAATCGCATGGTCTGCCGACGCGTCCATTAAAAGAT
TTGGGCAAGCGTGAGCTGTCGTTACTTTTGATGCTAGCAGCAGGCTTGGTCTGGCTGGGACTGTATCCACAGCCATTCCT
TGATACCTCAAGTCATGCGATGCAGTGGATTAATAACGCTTATATATATAGTCAAGTAACCCAAGTAGATGCCTCGCAGC
TGCTTGATGCAATGGAGGCACGTTAA

Upstream 100 bases:

>100_bases
TTGTCTGCGACGCAAACAGGGTCACTTCGAGGTTATGCTGCAAGCTTTGGCTTGGGTATGGCTGTATTACTCGTGCTGGT
AATGATGACGGTGGTATAAG

Downstream 100 bases:

>100_bases
GTCATGAACGATTTTACGATGAATGATTTGATGGGGCTACTGCCTTATGCACCAATCATTGCGGTAGTTATTACAGTATT
GGTGGTGATGATTGCCATCA

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MIELQQTWMLPALIAIPFIAGLLCWLVERFNKRLPRWIALIGMTLTFVLSIVLWQYGDFSGMSQQVIAPDAAVPWVAEFS
VPWIPSFGISFHLALDGLSLMMVALTGLLGVAAVACSWNEIQRRVGFFHLNLLWSLGGVIGVFLAIDMFLFFFFWEMMLV
PIYFLIAIWGHDVVGKTKEYAATKFFIYTQASGLIMLVGILILVIISYAQKGVVSFNYNDLLGTSLGGWEYPIMLCFFIG
FAVKLPIIPFHGWLPDAHAQAPTAGSVDLAGVLIKTAAYGLIRFVLPLFPAASQDFAPIAMTLGTIGIFYGAWLAFMQTD
MKRLLAYTSISHMGFVVLAIYAGTLLSLQGLMIQMLAHGLSSAALFIMAGQLYERLHTRDLTLMGGMWGQFRYYAPILMF
FCAALLGIPGTGNFIGEFLILFGAFAQYPVFVVLATFSLVLAGLYSLILIHKALFGINNIEEVALKETKSHGLPTRPLKD
LGKRELSLLLMLAAGLVWLGLYPQPFLDTSSHAMQWINNAYIYSQVTQVDASQLLDAMEAR

Sequences:

>Translated_541_residues
MIELQQTWMLPALIAIPFIAGLLCWLVERFNKRLPRWIALIGMTLTFVLSIVLWQYGDFSGMSQQVIAPDAAVPWVAEFS
VPWIPSFGISFHLALDGLSLMMVALTGLLGVAAVACSWNEIQRRVGFFHLNLLWSLGGVIGVFLAIDMFLFFFFWEMMLV
PIYFLIAIWGHDVVGKTKEYAATKFFIYTQASGLIMLVGILILVIISYAQKGVVSFNYNDLLGTSLGGWEYPIMLCFFIG
FAVKLPIIPFHGWLPDAHAQAPTAGSVDLAGVLIKTAAYGLIRFVLPLFPAASQDFAPIAMTLGTIGIFYGAWLAFMQTD
MKRLLAYTSISHMGFVVLAIYAGTLLSLQGLMIQMLAHGLSSAALFIMAGQLYERLHTRDLTLMGGMWGQFRYYAPILMF
FCAALLGIPGTGNFIGEFLILFGAFAQYPVFVVLATFSLVLAGLYSLILIHKALFGINNIEEVALKETKSHGLPTRPLKD
LGKRELSLLLMLAAGLVWLGLYPQPFLDTSSHAMQWINNAYIYSQVTQVDASQLLDAMEAR
>Mature_541_residues
MIELQQTWMLPALIAIPFIAGLLCWLVERFNKRLPRWIALIGMTLTFVLSIVLWQYGDFSGMSQQVIAPDAAVPWVAEFS
VPWIPSFGISFHLALDGLSLMMVALTGLLGVAAVACSWNEIQRRVGFFHLNLLWSLGGVIGVFLAIDMFLFFFFWEMMLV
PIYFLIAIWGHDVVGKTKEYAATKFFIYTQASGLIMLVGILILVIISYAQKGVVSFNYNDLLGTSLGGWEYPIMLCFFIG
FAVKLPIIPFHGWLPDAHAQAPTAGSVDLAGVLIKTAAYGLIRFVLPLFPAASQDFAPIAMTLGTIGIFYGAWLAFMQTD
MKRLLAYTSISHMGFVVLAIYAGTLLSLQGLMIQMLAHGLSSAALFIMAGQLYERLHTRDLTLMGGMWGQFRYYAPILMF
FCAALLGIPGTGNFIGEFLILFGAFAQYPVFVVLATFSLVLAGLYSLILIHKALFGINNIEEVALKETKSHGLPTRPLKD
LGKRELSLLLMLAAGLVWLGLYPQPFLDTSSHAMQWINNAYIYSQVTQVDASQLLDAMEAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=356, Percent_Identity=32.5842696629214, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1788613, Length=522, Percent_Identity=60.9195402298851, Blast_Score=610, Evalue=1e-176,
Organism=Escherichia coli, GI1788827, Length=309, Percent_Identity=27.831715210356, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788831, Length=192, Percent_Identity=32.2916666666667, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI145693160, Length=400, Percent_Identity=27, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788614, Length=412, Percent_Identity=26.2135922330097, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 59892; Mature: 59892

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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C
>Mature Secondary Structure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C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]