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The map label for this gene is nuoB [H]

Identifier: 93005404

GI number: 93005404

Start: 684499

End: 685167

Strand: Direct

Name: nuoB [H]

Synonym: Pcryo_0574

Alternate gene names: 93005404

Gene position: 684499-685167 (Clockwise)

Preceding gene: 93005403

Following gene: 93005405

Centisome position: 22.37

GC content: 46.19

Gene sequence:

>669_bases
ATGAAATACACATTAACAAAAGCCAACCCTGATGCAGATGTCTATCCTGCTCAGACAAGCCAAACTGTCAACGATCCAAT
GGAAGATGAAGTCAATAAAAACGTCTTTATGGGTCGTCTAGAAGATCTTGTGCATTCAACAGCAAACTGGGGGCGCAAGC
ACTCATTATGGCCATTTAACTTTGGTACCTCATGCTGTTATGTCGAATATGCGACTACTTTAACGGCGGTTCACGATTTG
TCACGCTTTGGTGCCGAAGTTATTCGCGCCTCGCCACGTCAGGCAGATGTGATGATTGTTGCTGGTACTTGCTTTGTTAA
AATGGCACCTGTTATTCAACGTCTATACGAGCAAATGCTTGAGCCAAAATGGGTCATCTCGATGGGTGCTTGTGCCAACT
CGGGCGGTATGTATGACATCTATTCGGTCGTGCAAGGCGTGGATAAAATTATCCCTGTCGATGTCTATGTGCCCGGTTGT
CCGCCACGTCCAGAAGCATTGATTCAAGGCTTGATGTTGTTACAAGAATCTATTACTAAAGAACGTCGTCCGCTGGGTAT
CCATGTCAATGATCAGGGTGTCTATCAGCCGCAGATGACGCCTGAGCGTGACCGTAAGCAAGCAGATCGTATCGCTGTGA
AAAACTTACGTAGCCCAGATAGCATTTAG

Upstream 100 bases:

>100_bases
CGTGTTAATTAGTGAATAACAAAATTATTTAATAATGAAGTTAAGGCAGTAGTGAGGGCAAATCATTGCCGCACTTTGTT
TAACATAAAGGTTGTATGTT

Downstream 100 bases:

>100_bases
TTAAGCATTTTGGCGTAGTTATTAATAATTGTTAGAAGTGCGCGTACGCTCATCATAGTTTGGTTATGATTATCATCTTT
TATTATGGTTGTCATGGTTC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKYTLTKANPDADVYPAQTSQTVNDPMEDEVNKNVFMGRLEDLVHSTANWGRKHSLWPFNFGTSCCYVEYATTLTAVHDL
SRFGAEVIRASPRQADVMIVAGTCFVKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGC
PPRPEALIQGLMLLQESITKERRPLGIHVNDQGVYQPQMTPERDRKQADRIAVKNLRSPDSI

Sequences:

>Translated_222_residues
MKYTLTKANPDADVYPAQTSQTVNDPMEDEVNKNVFMGRLEDLVHSTANWGRKHSLWPFNFGTSCCYVEYATTLTAVHDL
SRFGAEVIRASPRQADVMIVAGTCFVKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGC
PPRPEALIQGLMLLQESITKERRPLGIHVNDQGVYQPQMTPERDRKQADRIAVKNLRSPDSI
>Mature_222_residues
MKYTLTKANPDADVYPAQTSQTVNDPMEDEVNKNVFMGRLEDLVHSTANWGRKHSLWPFNFGTSCCYVEYATTLTAVHDL
SRFGAEVIRASPRQADVMIVAGTCFVKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGC
PPRPEALIQGLMLLQESITKERRPLGIHVNDQGVYQPQMTPERDRKQADRIAVKNLRSPDSI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=148, Percent_Identity=54.7297297297297, Blast_Score=171, Evalue=3e-43,
Organism=Escherichia coli, GI1788624, Length=224, Percent_Identity=72.7678571428571, Blast_Score=347, Evalue=4e-97,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=43.4426229508197, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788834, Length=161, Percent_Identity=37.2670807453416, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=163, Percent_Identity=47.8527607361963, Blast_Score=159, Evalue=8e-40,
Organism=Drosophila melanogaster, GI18859983, Length=147, Percent_Identity=55.1020408163265, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24642371, Length=147, Percent_Identity=55.1020408163265, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=56.551724137931, Blast_Score=170, Evalue=6e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 24880; Mature: 24880

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTLTKANPDADVYPAQTSQTVNDPMEDEVNKNVFMGRLEDLVHSTANWGRKHSLWPFN
CCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FGTSCCYVEYATTLTAVHDLSRFGAEVIRASPRQADVMIVAGTCFVKMAPVIQRLYEQML
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
EPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGCPPRPEALIQGLMLLQESITK
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ERRPLGIHVNDQGVYQPQMTPERDRKQADRIAVKNLRSPDSI
HCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKYTLTKANPDADVYPAQTSQTVNDPMEDEVNKNVFMGRLEDLVHSTANWGRKHSLWPFN
CCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
FGTSCCYVEYATTLTAVHDLSRFGAEVIRASPRQADVMIVAGTCFVKMAPVIQRLYEQML
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHC
EPKWVISMGACANSGGMYDIYSVVQGVDKIIPVDVYVPGCPPRPEALIQGLMLLQESITK
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
ERRPLGIHVNDQGVYQPQMTPERDRKQADRIAVKNLRSPDSI
HCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA