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The map label for this gene is pyrB [H]

Identifier: 93005361

GI number: 93005361

Start: 627728

End: 628801

Strand: Direct

Name: pyrB [H]

Synonym: Pcryo_0531

Alternate gene names: 93005361

Gene position: 627728-628801 (Clockwise)

Preceding gene: 93005358

Following gene: 93005362

Centisome position: 20.51

GC content: 47.02

Gene sequence:

>1074_bases
ATGTCAAATTCTATCGATAGCCAATCACTACCTACCGTCTCGCCGACAGACTACGCAAGATTTGATCCTGATACGATTCA
TGAAAAACTTGATGCGTCATTGAGTCGTCCGCAGCTCAATACTGATGGTAGTATTCGTCATTTTTTAGGTATCGAAGGTC
TTAATAAAGCCCAGCTGCAGGCGATTATCGCTAAAGCTGAGTCATTTTTTGATGATAAGGGACAGCTGATTAACCGTCCT
GAGCTTGAAGGCTATACGGTGATGAATCTGTTTTTTGAGCCATCGACGCGCACACGTACCACGTTTGAAGTAGCAGAAAA
ACGTTTAGGCGCTAATGTATTAAATATCGATATCGAACGCTCTAGCACCAAAAAAGGCGAAAGCTTACGCGATACGTTAT
GGAATCTGCAAGCGATGACGGCAGATATCTTTGTGGTACGTCATTCAGCATCAGGTGCGGCACATTTTATGGCGACTGAA
GTGACGCCAGATATTGCCATTATCAATGGTGGTGATGGTTGGCATGCGCATCCAACGCAAGGCATGCTTGATATGCTGAC
TATTCACCGTGAGGCGCCGCGTCCTTTTGAAGAGCTGTCGGTTGCAATCGTTGGCGATATCAAACACTCGCGAGTAGCAC
GCTCTGACATCAGTGCGCTACAAACATTGGGTGTAAAAGACATTCGCGTCTGCGCACCTCGTACGTTATTACCAAAAGGG
ATTGAGCGTTTTGGTGTGCAAGTATATGAGAATATGAATGAATGCGTGACGGACTGTGATGTCATTATGGGCTTGAGAAT
TCAAAATGAGCGTATTGGCTCACCGCTCCTCGCCTCATCGAGTGAATACTATAAGCACTATGGTATTACTCCTGAGCGCG
TGGCACTGGCGAAGCCTGATGCTCTAGTTATGCACCCAGGACCGATGAACCGCGGTGTTGAGATTGCCTCAAGCGTTGCC
GATGGTGCGCAATCTGTGATTTTAAAACAGGTTAATAACGGCATTGCTATTCGTATGGCGGTACTGTCACTGGCGATGGA
AGGTCAGCGCGCGCATCAAGCGGCTGGTCATTAA

Upstream 100 bases:

>100_bases
TCTGTATGCATGTCTGCCGTATTATCGAGCCAAAACCGTTAAAAAAACGAATGATAACTTATTATAAAATCATACAAACC
CTTAATGAAGACGTGTCCTT

Downstream 100 bases:

>100_bases
TATTTAGCTCGCCTGTTTTATTCTTATTTATCCCATTTCATTGATACGGTAATAACGCTCATGGTTCACATTACGAATTC
AGATGTTCATATGCACGACT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSNSIDSQSLPTVSPTDYARFDPDTIHEKLDASLSRPQLNTDGSIRHFLGIEGLNKAQLQAIIAKAESFFDDKGQLINRP
ELEGYTVMNLFFEPSTRTRTTFEVAEKRLGANVLNIDIERSSTKKGESLRDTLWNLQAMTADIFVVRHSASGAAHFMATE
VTPDIAIINGGDGWHAHPTQGMLDMLTIHREAPRPFEELSVAIVGDIKHSRVARSDISALQTLGVKDIRVCAPRTLLPKG
IERFGVQVYENMNECVTDCDVIMGLRIQNERIGSPLLASSSEYYKHYGITPERVALAKPDALVMHPGPMNRGVEIASSVA
DGAQSVILKQVNNGIAIRMAVLSLAMEGQRAHQAAGH

Sequences:

>Translated_357_residues
MSNSIDSQSLPTVSPTDYARFDPDTIHEKLDASLSRPQLNTDGSIRHFLGIEGLNKAQLQAIIAKAESFFDDKGQLINRP
ELEGYTVMNLFFEPSTRTRTTFEVAEKRLGANVLNIDIERSSTKKGESLRDTLWNLQAMTADIFVVRHSASGAAHFMATE
VTPDIAIINGGDGWHAHPTQGMLDMLTIHREAPRPFEELSVAIVGDIKHSRVARSDISALQTLGVKDIRVCAPRTLLPKG
IERFGVQVYENMNECVTDCDVIMGLRIQNERIGSPLLASSSEYYKHYGITPERVALAKPDALVMHPGPMNRGVEIASSVA
DGAQSVILKQVNNGIAIRMAVLSLAMEGQRAHQAAGH
>Mature_356_residues
SNSIDSQSLPTVSPTDYARFDPDTIHEKLDASLSRPQLNTDGSIRHFLGIEGLNKAQLQAIIAKAESFFDDKGQLINRPE
LEGYTVMNLFFEPSTRTRTTFEVAEKRLGANVLNIDIERSSTKKGESLRDTLWNLQAMTADIFVVRHSASGAAHFMATEV
TPDIAIINGGDGWHAHPTQGMLDMLTIHREAPRPFEELSVAIVGDIKHSRVARSDISALQTLGVKDIRVCAPRTLLPKGI
ERFGVQVYENMNECVTDCDVIMGLRIQNERIGSPLLASSSEYYKHYGITPERVALAKPDALVMHPGPMNRGVEIASSVAD
GAQSVILKQVNNGIAIRMAVLSLAMEGQRAHQAAGH

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=32.7974276527331, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=31.8471337579618, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786469, Length=200, Percent_Identity=28, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1790703, Length=195, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=331, Percent_Identity=33.5347432024169, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=311, Percent_Identity=32.7974276527331, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=33.4394904458599, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 39137; Mature: 39006

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSIDSQSLPTVSPTDYARFDPDTIHEKLDASLSRPQLNTDGSIRHFLGIEGLNKAQLQ
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCHHHHH
AIIAKAESFFDDKGQLINRPELEGYTVMNLFFEPSTRTRTTFEVAEKRLGANVLNIDIER
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEEC
SSTKKGESLRDTLWNLQAMTADIFVVRHSASGAAHFMATEVTPDIAIINGGDGWHAHPTQ
CCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCC
GMLDMLTIHREAPRPFEELSVAIVGDIKHSRVARSDISALQTLGVKDIRVCAPRTLLPKG
HHHHHHHHHCCCCCCHHHCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHH
IERFGVQVYENMNECVTDCDVIMGLRIQNERIGSPLLASSSEYYKHYGITPERVALAKPD
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHCCCHHHHHHCCCCHHHEEEECCC
ALVMHPGPMNRGVEIASSVADGAQSVILKQVNNGIAIRMAVLSLAMEGQRAHQAAGH
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure 
SNSIDSQSLPTVSPTDYARFDPDTIHEKLDASLSRPQLNTDGSIRHFLGIEGLNKAQLQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEHHCCCCCCHHHHH
AIIAKAESFFDDKGQLINRPELEGYTVMNLFFEPSTRTRTTFEVAEKRLGANVLNIDIER
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEEC
SSTKKGESLRDTLWNLQAMTADIFVVRHSASGAAHFMATEVTPDIAIINGGDGWHAHPTQ
CCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCCC
GMLDMLTIHREAPRPFEELSVAIVGDIKHSRVARSDISALQTLGVKDIRVCAPRTLLPKG
HHHHHHHHHCCCCCCHHHCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHH
IERFGVQVYENMNECVTDCDVIMGLRIQNERIGSPLLASSSEYYKHYGITPERVALAKPD
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHCCCHHHHHHCCCCHHHEEEECCC
ALVMHPGPMNRGVEIASSVADGAQSVILKQVNNGIAIRMAVLSLAMEGQRAHQAAGH
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA