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The map label for this gene is rpoA

Identifier: 93005339

GI number: 93005339

Start: 605553

End: 606560

Strand: Direct

Name: rpoA

Synonym: Pcryo_0509

Alternate gene names: 93005339

Gene position: 605553-606560 (Clockwise)

Preceding gene: 93005338

Following gene: 93005340

Centisome position: 19.79

GC content: 43.25

Gene sequence:

>1008_bases
ATGATGCTAAATGCAACTGAGTTTCTAACGCCGAACGCCATCAACGTGGATACGGTTAATGAAACGATTGCGAAAGTCAC
GCTCGAACCGTTAGAACGCGGCTTTGGGCATACCTTGGGTAATGCCTTACGTCGCATCTTGTTATCTTCATTACCTGGTG
CTGCAGTCATTGAAGCTGAGATTGATGGTGTTGACCATGAATACTCAACGCTTGAAGGACTACAAGAAGATGTGCTTGAT
TTGCTTTTGAACTTAAAAGGCTTAGCAATCACCCTTCATGACCAAAATGAAGTATTTTTGACCTTGGATAAACAAGGTCC
AGGCACTATTACTGCTGCAGACATTACGTTACCGCACAATGTAGACATCATCAATCCAGAATTGGTATTGGGTACATTGA
GTGACCGTGGTCATCTAAAGATGCGTTTGCGCGTAGTAATGGGTCGTGGATATGAGCCAGCAAACCAGCGCCGTGAAGAT
GGTGATACTAAAGCGATTGGACGCTTGAAGCTTGATGCAAGTTTTAGTCCTGTGCTTCGTGTTGCTTATCAGGTTGAGAA
CGCACGTGTAGAACAACGTACCGATCTTGATCGTCTTATCATTGAGCTTGAAACAAATGGCACTATAGATCCAGAAGAAG
CAATTCGTAAAGCAGCCACTATATTACAACAACAGATTTCTATCTTTGTTGACCTAGAAGCTGAAGAAGCGCCTGAGCCT
GTGAAAGAAAAAGAAGAGGTTGATCCGGTGCTATTACGCCCTGTGGACGATCTTGAACTAACGGTTCGCTCAGCCAACTG
CTTAAAAGCTGAAAACATTTACTATATCGGTGATTTGGTACAGCGTTCAGAGACTGAACTTCTAAAAACCCCAAATCTTG
GTAAGAAATCATTGACAGAAATCAAAGACGTACTAGCGTCTAAAGATTTGGAGCTTGGTATGCGCCTAGATAACTGGCCA
CCAGCTGATTTACGTGTTGATGATCGCTTTTCTTATCGTAGCCGTTAA

Upstream 100 bases:

>100_bases
TGAGCTATACTCTAAGTAAGTAACGTACTGCTCGATGCTGTTCACATGGCATTGAGCAATTAACGTTAATTAAACCAGTT
TAATAAATCGAGGTGACATC

Downstream 100 bases:

>100_bases
ACTTTAAGGATTTTTGACTATGCGCCATCGTAAGAGTGGAGTCAAGCTGGGTCGTACCGGCAGTCATCGTAAGGCAATGT
TTCAGAACATGACCAACTCA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MMLNATEFLTPNAINVDTVNETIAKVTLEPLERGFGHTLGNALRRILLSSLPGAAVIEAEIDGVDHEYSTLEGLQEDVLD
LLLNLKGLAITLHDQNEVFLTLDKQGPGTITAADITLPHNVDIINPELVLGTLSDRGHLKMRLRVVMGRGYEPANQRRED
GDTKAIGRLKLDASFSPVLRVAYQVENARVEQRTDLDRLIIELETNGTIDPEEAIRKAATILQQQISIFVDLEAEEAPEP
VKEKEEVDPVLLRPVDDLELTVRSANCLKAENIYYIGDLVQRSETELLKTPNLGKKSLTEIKDVLASKDLELGMRLDNWP
PADLRVDDRFSYRSR

Sequences:

>Translated_335_residues
MMLNATEFLTPNAINVDTVNETIAKVTLEPLERGFGHTLGNALRRILLSSLPGAAVIEAEIDGVDHEYSTLEGLQEDVLD
LLLNLKGLAITLHDQNEVFLTLDKQGPGTITAADITLPHNVDIINPELVLGTLSDRGHLKMRLRVVMGRGYEPANQRRED
GDTKAIGRLKLDASFSPVLRVAYQVENARVEQRTDLDRLIIELETNGTIDPEEAIRKAATILQQQISIFVDLEAEEAPEP
VKEKEEVDPVLLRPVDDLELTVRSANCLKAENIYYIGDLVQRSETELLKTPNLGKKSLTEIKDVLASKDLELGMRLDNWP
PADLRVDDRFSYRSR
>Mature_335_residues
MMLNATEFLTPNAINVDTVNETIAKVTLEPLERGFGHTLGNALRRILLSSLPGAAVIEAEIDGVDHEYSTLEGLQEDVLD
LLLNLKGLAITLHDQNEVFLTLDKQGPGTITAADITLPHNVDIINPELVLGTLSDRGHLKMRLRVVMGRGYEPANQRRED
GDTKAIGRLKLDASFSPVLRVAYQVENARVEQRTDLDRLIIELETNGTIDPEEAIRKAATILQQQISIFVDLEAEEAPEP
VKEKEEVDPVLLRPVDDLELTVRSANCLKAENIYYIGDLVQRSETELLKTPNLGKKSLTEIKDVLASKDLELGMRLDNWP
PADLRVDDRFSYRSR

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=326, Percent_Identity=66.2576687116564, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_PSYA2 (Q4FUD1)

Other databases:

- EMBL:   CP000082
- RefSeq:   YP_263811.1
- ProteinModelPortal:   Q4FUD1
- STRING:   Q4FUD1
- GeneID:   3514313
- GenomeReviews:   CP000082_GR
- KEGG:   par:Psyc_0514
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q4FUD1
- ProtClustDB:   PRK05182
- BioCyc:   PARC259536:PSYC_0514-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37373; Mature: 37373

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLNATEFLTPNAINVDTVNETIAKVTLEPLERGFGHTLGNALRRILLSSLPGAAVIEAE
CCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVDHEYSTLEGLQEDVLDLLLNLKGLAITLHDQNEVFLTLDKQGPGTITAADITLPHN
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCEEEEEEEECCCC
VDIINPELVLGTLSDRGHLKMRLRVVMGRGYEPANQRREDGDTKAIGRLKLDASFSPVLR
CEEECCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
VAYQVENARVEQRTDLDRLIIELETNGTIDPEEAIRKAATILQQQISIFVDLEAEEAPEP
HHHHHHCCHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCC
VKEKEEVDPVLLRPVDDLELTVRSANCLKAENIYYIGDLVQRSETELLKTPNLGKKSLTE
CHHHHCCCCEEECCCCCCEEEEECCCCEEECCEEEEHHHHHCCCHHHHCCCCCCHHHHHH
IKDVLASKDLELGMRLDNWPPADLRVDDRFSYRSR
HHHHHHCCCCCCCCEECCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MMLNATEFLTPNAINVDTVNETIAKVTLEPLERGFGHTLGNALRRILLSSLPGAAVIEAE
CCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEE
IDGVDHEYSTLEGLQEDVLDLLLNLKGLAITLHDQNEVFLTLDKQGPGTITAADITLPHN
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCEEEEEEEECCCC
VDIINPELVLGTLSDRGHLKMRLRVVMGRGYEPANQRREDGDTKAIGRLKLDASFSPVLR
CEEECCEEEEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
VAYQVENARVEQRTDLDRLIIELETNGTIDPEEAIRKAATILQQQISIFVDLEAEEAPEP
HHHHHHCCHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCC
VKEKEEVDPVLLRPVDDLELTVRSANCLKAENIYYIGDLVQRSETELLKTPNLGKKSLTE
CHHHHCCCCEEECCCCCCEEEEECCCCEEECCEEEEHHHHHCCCHHHHCCCCCCHHHHHH
IKDVLASKDLELGMRLDNWPPADLRVDDRFSYRSR
HHHHHHCCCCCCCCEECCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA