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The map label for this gene is folC [H]

Identifier: 93005300

GI number: 93005300

Start: 576586

End: 577959

Strand: Direct

Name: folC [H]

Synonym: Pcryo_0470

Alternate gene names: 93005300

Gene position: 576586-577959 (Clockwise)

Preceding gene: 93005299

Following gene: 93005301

Centisome position: 18.84

GC content: 45.71

Gene sequence:

>1374_bases
ATGTCTGATTCTTTAGTGTCTCGTAGCCCCAGTAGTACCCCTGACAACCAAGCCACTTTAACCGAATGGCTGAATTATAT
GCAGCAGATTCATGTCTCGGCGATAGATATGGGGCTGTCACGAGTACTGCCAGTTGCTGAGGCATTGGGAGTCGTACAAT
CAGCCAAAGATGACGCCTATGTATTTACGGTTGCAGGTACTAATGGCAAAGGCTCGACGACTGCGGTCATCGCGCAGATG
TGCCAAGCCGCAGGATATAAAACTGCCTTATATCAATCGCCGCACTTGAGCGTCTTTAACGAGCGGGTACGGATCGATGG
CGAGATGGTCAGCGATGAAACCCTGATTGCTGCCTTTAGCAAGGTAGAAGCGGCACGGTTGCAATGTGATTTAACATTGT
CATTTTTTGAGATGACAACACTGGCAGCATTGTTGATATTCGCGGAAGCGGATTGCGATGTTTGGGTGTTAGAAGTAGGG
CTGGGCGGTCGCTTAGACGTGGTCAATATCATCGACCCTGATATGGCAGTGATTACCAATATTGCCATCGATCATGTTGA
TTGGTTGGGTGATAATGTGGAAGCCATCGGAGCGGAAAAAGCAGGTATTTTACGTGATGGGATTAGCGTCATTTACGGTG
CTACAGAGATGCCAAATAGCGTACAGCAAGCGATAGATAAGCATCAGGCGACTTGTTATCAAGTCAGTAAAGAGTTCAGC
TATTGTGAAAATAATTCGACTAGGTGGCAATATAGCAATGCCGCTGTCACTCTACAATTGCCTCGACCCGCACTGTCATT
GACCAATACTGCCAATGCTCTGTCAGCAGTACTTGCAAGTCCCTTAGACGTCGATACCAATGCTATAGAGCAAGCGCTAA
AAACAGTTAAGCTTGCCGGTCGCTTCGATTATCGTGAAGTCCATGAGCGTCATTGGCTATTTGATGTGGCGCATAATGAT
CAAGGCGTTGAGTTTTTATTGGCACAGTTAGTGCCGCTTTGGCAGCAGCATTTAACACAGCAAAGTACATCTGAACAAGT
TAATCAAACTTCAGGTGAACCTGCTACCATTAAAATGTTGTTTTCTATGCTAGGTGATAAAGATATTAATAAAGTGGTGC
AGCGTTTGACGACAGCAGGCTTACCAATCAGTGATTGGTTCATCGCTGAAATTGATTATCCACGTGCTGCCACTACGGAG
CATTTGCAAGGTATCTTAGCGAGCTATGTCGATGATTCTCAGATACATGAGTTTGCGCGTTTGCAAGAAGCCACTCATGC
GATTATAAATGCCAGTCAGCCACAAGATTTAATCGTAGTCTGTGGCTCTTTTCATACGATTGGAGAGGCATTGTCTACGC
TTGAAACGCGATAA

Upstream 100 bases:

>100_bases
GGTAAATGCTATTTTTGCTTTGTAGCAAGCTAGTCATACTGTTTTTATTTTTTTATATAAGTTTTATCTTTTATCTCAAA
CTTTGATATAGGTTCTGTCC

Downstream 100 bases:

>100_bases
TTTACGTTAAAACATTGACTGTTTGATGTTAGTCTTAGTAATAGCGTGGTGGCAGACAGCATGAAAAATATGCTTTATAA
TCAAAGTGATTAAAACCTAG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSDSLVSRSPSSTPDNQATLTEWLNYMQQIHVSAIDMGLSRVLPVAEALGVVQSAKDDAYVFTVAGTNGKGSTTAVIAQM
CQAAGYKTALYQSPHLSVFNERVRIDGEMVSDETLIAAFSKVEAARLQCDLTLSFFEMTTLAALLIFAEADCDVWVLEVG
LGGRLDVVNIIDPDMAVITNIAIDHVDWLGDNVEAIGAEKAGILRDGISVIYGATEMPNSVQQAIDKHQATCYQVSKEFS
YCENNSTRWQYSNAAVTLQLPRPALSLTNTANALSAVLASPLDVDTNAIEQALKTVKLAGRFDYREVHERHWLFDVAHND
QGVEFLLAQLVPLWQQHLTQQSTSEQVNQTSGEPATIKMLFSMLGDKDINKVVQRLTTAGLPISDWFIAEIDYPRAATTE
HLQGILASYVDDSQIHEFARLQEATHAIINASQPQDLIVVCGSFHTIGEALSTLETR

Sequences:

>Translated_457_residues
MSDSLVSRSPSSTPDNQATLTEWLNYMQQIHVSAIDMGLSRVLPVAEALGVVQSAKDDAYVFTVAGTNGKGSTTAVIAQM
CQAAGYKTALYQSPHLSVFNERVRIDGEMVSDETLIAAFSKVEAARLQCDLTLSFFEMTTLAALLIFAEADCDVWVLEVG
LGGRLDVVNIIDPDMAVITNIAIDHVDWLGDNVEAIGAEKAGILRDGISVIYGATEMPNSVQQAIDKHQATCYQVSKEFS
YCENNSTRWQYSNAAVTLQLPRPALSLTNTANALSAVLASPLDVDTNAIEQALKTVKLAGRFDYREVHERHWLFDVAHND
QGVEFLLAQLVPLWQQHLTQQSTSEQVNQTSGEPATIKMLFSMLGDKDINKVVQRLTTAGLPISDWFIAEIDYPRAATTE
HLQGILASYVDDSQIHEFARLQEATHAIINASQPQDLIVVCGSFHTIGEALSTLETR
>Mature_456_residues
SDSLVSRSPSSTPDNQATLTEWLNYMQQIHVSAIDMGLSRVLPVAEALGVVQSAKDDAYVFTVAGTNGKGSTTAVIAQMC
QAAGYKTALYQSPHLSVFNERVRIDGEMVSDETLIAAFSKVEAARLQCDLTLSFFEMTTLAALLIFAEADCDVWVLEVGL
GGRLDVVNIIDPDMAVITNIAIDHVDWLGDNVEAIGAEKAGILRDGISVIYGATEMPNSVQQAIDKHQATCYQVSKEFSY
CENNSTRWQYSNAAVTLQLPRPALSLTNTANALSAVLASPLDVDTNAIEQALKTVKLAGRFDYREVHERHWLFDVAHNDQ
GVEFLLAQLVPLWQQHLTQQSTSEQVNQTSGEPATIKMLFSMLGDKDINKVVQRLTTAGLPISDWFIAEIDYPRAATTEH
LQGILASYVDDSQIHEFARLQEATHAIINASQPQDLIVVCGSFHTIGEALSTLETR

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=351, Percent_Identity=27.9202279202279, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI66932990, Length=351, Percent_Identity=27.9202279202279, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1788654, Length=446, Percent_Identity=37.6681614349776, Blast_Score=281, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17553150, Length=325, Percent_Identity=27.0769230769231, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17553148, Length=325, Percent_Identity=27.0769230769231, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=325, Percent_Identity=27.0769230769231, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=444, Percent_Identity=27.2522522522523, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324815, Length=306, Percent_Identity=28.7581699346405, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24641571, Length=185, Percent_Identity=34.5945945945946, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581568, Length=182, Percent_Identity=30.2197802197802, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 49992; Mature: 49861

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLVSRSPSSTPDNQATLTEWLNYMQQIHVSAIDMGLSRVLPVAEALGVVQSAKDDAY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VFTVAGTNGKGSTTAVIAQMCQAAGYKTALYQSPHLSVFNERVRIDGEMVSDETLIAAFS
EEEEECCCCCCHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHEECCCCCCCHHHHHHHH
KVEAARLQCDLTLSFFEMTTLAALLIFAEADCDVWVLEVGLGGRLDVVNIIDPDMAVITN
HHHHHHHEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHH
IAIDHVDWLGDNVEAIGAEKAGILRDGISVIYGATEMPNSVQQAIDKHQATCYQVSKEFS
HHHHHHHHCCCCHHHCCCCHHHHHHHCHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
YCENNSTRWQYSNAAVTLQLPRPALSLTNTANALSAVLASPLDVDTNAIEQALKTVKLAG
HHCCCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
RFDYREVHERHWLFDVAHNDQGVEFLLAQLVPLWQQHLTQQSTSEQVNQTSGEPATIKML
CCCHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FSMLGDKDINKVVQRLTTAGLPISDWFIAEIDYPRAATTEHLQGILASYVDDSQIHEFAR
HHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LQEATHAIINASQPQDLIVVCGSFHTIGEALSTLETR
HHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSLVSRSPSSTPDNQATLTEWLNYMQQIHVSAIDMGLSRVLPVAEALGVVQSAKDDAY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VFTVAGTNGKGSTTAVIAQMCQAAGYKTALYQSPHLSVFNERVRIDGEMVSDETLIAAFS
EEEEECCCCCCHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHEECCCCCCCHHHHHHHH
KVEAARLQCDLTLSFFEMTTLAALLIFAEADCDVWVLEVGLGGRLDVVNIIDPDMAVITN
HHHHHHHEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHH
IAIDHVDWLGDNVEAIGAEKAGILRDGISVIYGATEMPNSVQQAIDKHQATCYQVSKEFS
HHHHHHHHCCCCHHHCCCCHHHHHHHCHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
YCENNSTRWQYSNAAVTLQLPRPALSLTNTANALSAVLASPLDVDTNAIEQALKTVKLAG
HHCCCCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
RFDYREVHERHWLFDVAHNDQGVEFLLAQLVPLWQQHLTQQSTSEQVNQTSGEPATIKML
CCCHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
FSMLGDKDINKVVQRLTTAGLPISDWFIAEIDYPRAATTEHLQGILASYVDDSQIHEFAR
HHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LQEATHAIINASQPQDLIVVCGSFHTIGEALSTLETR
HHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]