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The map label for this gene is yrbD [H]

Identifier: 93005282

GI number: 93005282

Start: 554939

End: 556489

Strand: Reverse

Name: yrbD [H]

Synonym: Pcryo_0452

Alternate gene names: 93005282

Gene position: 556489-554939 (Counterclockwise)

Preceding gene: 93005287

Following gene: 93005279

Centisome position: 18.19

GC content: 44.04

Gene sequence:

>1551_bases
ATGGAAGGTTTAGTCAATTTAGTAAACGGAATTATTTGGAGCCCTGCATTAATTTACTTATGCTTGGGCGCAGGTCTATT
TTATTCCATCATGACACGCTTTGTACAAGTACGTTTATTCGGTGAAATGGTCAGACTACTATTCACTGGTAAATCCAGTG
CAGATGGTATCTCTTCATTCCAAGCCCTGGCAGTAGCCTTAGCTGGGCGTGTGGGTATGGGTAACATCGCAGGGGTAGCT
GCGGCTATCGGTTTCGGTGGTCCTGGTGCCGTATTTTGGATGTGGGTTGTGGCGTTCTTAGGAGCTTCTACTGCTTACGT
TGAATCTACGCTGGCACAGATTTATAAAGAAAGAGACGTCATCACTGGTGAATATCGCGGTGGCCCTGCTTACTATTTTG
AGCGGGCACTTGGTCAAAAATGGTACGGTATCCTCTTCGCAATCGCTTCTATCTTAGCTTGTGGTATGTTCTTACCAGGT
GTACAGGCAAATGGCGTTCTTAGTGCTTTCACGCAACTTATGGGTGAAGGTACTACTATGAACGTTGCAGGCCTAGAAGT
CGGCTCTATGCGTCTGATCGGCTTAGCCATCATCTTAGTCGTACTAGGTATCATCATTTTTGGTGGTATCAAGCGTATCG
CAACGTTTACCGAGTATGCGGTTCCTTTCATGGCAGTCGGTTATATTGTCTTAGCTTTAATTATCATGTTCAGCAACTTC
AGTTTGATCCCAGATATATTTGGTTTGATCATTAGTGATGCCTTTACGGCGCAAGCTGGCTTTGGTGCTGCTATCGGTTG
GGGTGTAAAACGTGGTATTTACTCTAACGAAGCCGGTCAAGGTACAGGTCCTCACGCTGCTGCTGCTGCTGAAGTTCAGC
ATCCATCACAGCAGGGTCTAGTACAGGCCTTCTCAGTATATGTTGATACACTACTGGTTTGTTCTGCTACTGCCTTTATG
ATCTTGTCTACTGGTATGTACAATATTCAGGGTACTTTGCCAGAAGGTCAGTTCATTCTGCAAAATGTAGCCGCTACTAC
TGAAATCAATTCGCCTGCCTTTACCCAAATGGCAATGGAATCAGTATATGGTACTACTGGTAATGTCTTTATTGCAGTCG
CTGTATTCTTCTTTGCCTTTACCACTATCTTGGCTTACTACTACATCGCTGAAGTGAACGTTGCTTACTTGACTCGCTTT
ATCGGGCGCGGTGCAAATAAGACAGGTTTATTCTTAGTAAAAATCTTAATCATGGTAATGGTTGCTTACGGTGCTCTAAA
CTCAGCTGGTTACATCTGGGCAATTGGTGATATCGGTGTTGGCCTCATGGCTTGGCTCAATATTGTTGGTATTCTTGTTA
TCTTCTTTGTGGCTCGTCCGACGCTTACCATGCTTAAAGACTATGAAGCACAGCTTAAAGCAGGTGTAACACGCTATACA
TTTGACCCTGCAAAATTCGGTATCAAAAACGCGCCTTATTGGGAAGAGCGTCATCTAAAAGAACAACAAAGAATAGCGGC
ACAAGATCCTTTAAATAAAGAGCATCGTTAA

Upstream 100 bases:

>100_bases
GATAATTTGAGTGGTTGGCTTTAATAAGGATATTAAACCGTTAACTCACTCTATGCATCCTCATATATAGTCATTTTGAG
ACAGTTGAAGGAATAGATAT

Downstream 100 bases:

>100_bases
TCCTCTGTAAAGTATTTGCCTACTAGCCAGTTATTAAAAAAGCCCGAGACCATTGTCTTGGGTTTTTTTGTATGTGCGCT
CTTGCCTCTTTTCTACTATA

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MEGLVNLVNGIIWSPALIYLCLGAGLFYSIMTRFVQVRLFGEMVRLLFTGKSSADGISSFQALAVALAGRVGMGNIAGVA
AAIGFGGPGAVFWMWVVAFLGASTAYVESTLAQIYKERDVITGEYRGGPAYYFERALGQKWYGILFAIASILACGMFLPG
VQANGVLSAFTQLMGEGTTMNVAGLEVGSMRLIGLAIILVVLGIIIFGGIKRIATFTEYAVPFMAVGYIVLALIIMFSNF
SLIPDIFGLIISDAFTAQAGFGAAIGWGVKRGIYSNEAGQGTGPHAAAAAEVQHPSQQGLVQAFSVYVDTLLVCSATAFM
ILSTGMYNIQGTLPEGQFILQNVAATTEINSPAFTQMAMESVYGTTGNVFIAVAVFFFAFTTILAYYYIAEVNVAYLTRF
IGRGANKTGLFLVKILIMVMVAYGALNSAGYIWAIGDIGVGLMAWLNIVGILVIFFVARPTLTMLKDYEAQLKAGVTRYT
FDPAKFGIKNAPYWEERHLKEQQRIAAQDPLNKEHR

Sequences:

>Translated_516_residues
MEGLVNLVNGIIWSPALIYLCLGAGLFYSIMTRFVQVRLFGEMVRLLFTGKSSADGISSFQALAVALAGRVGMGNIAGVA
AAIGFGGPGAVFWMWVVAFLGASTAYVESTLAQIYKERDVITGEYRGGPAYYFERALGQKWYGILFAIASILACGMFLPG
VQANGVLSAFTQLMGEGTTMNVAGLEVGSMRLIGLAIILVVLGIIIFGGIKRIATFTEYAVPFMAVGYIVLALIIMFSNF
SLIPDIFGLIISDAFTAQAGFGAAIGWGVKRGIYSNEAGQGTGPHAAAAAEVQHPSQQGLVQAFSVYVDTLLVCSATAFM
ILSTGMYNIQGTLPEGQFILQNVAATTEINSPAFTQMAMESVYGTTGNVFIAVAVFFFAFTTILAYYYIAEVNVAYLTRF
IGRGANKTGLFLVKILIMVMVAYGALNSAGYIWAIGDIGVGLMAWLNIVGILVIFFVARPTLTMLKDYEAQLKAGVTRYT
FDPAKFGIKNAPYWEERHLKEQQRIAAQDPLNKEHR
>Mature_516_residues
MEGLVNLVNGIIWSPALIYLCLGAGLFYSIMTRFVQVRLFGEMVRLLFTGKSSADGISSFQALAVALAGRVGMGNIAGVA
AAIGFGGPGAVFWMWVVAFLGASTAYVESTLAQIYKERDVITGEYRGGPAYYFERALGQKWYGILFAIASILACGMFLPG
VQANGVLSAFTQLMGEGTTMNVAGLEVGSMRLIGLAIILVVLGIIIFGGIKRIATFTEYAVPFMAVGYIVLALIIMFSNF
SLIPDIFGLIISDAFTAQAGFGAAIGWGVKRGIYSNEAGQGTGPHAAAAAEVQHPSQQGLVQAFSVYVDTLLVCSATAFM
ILSTGMYNIQGTLPEGQFILQNVAATTEINSPAFTQMAMESVYGTTGNVFIAVAVFFFAFTTILAYYYIAEVNVAYLTRF
IGRGANKTGLFLVKILIMVMVAYGALNSAGYIWAIGDIGVGLMAWLNIVGILVIFFVARPTLTMLKDYEAQLKAGVTRYT
FDPAKFGIKNAPYWEERHLKEQQRIAAQDPLNKEHR

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=500, Percent_Identity=35.8, Blast_Score=254, Evalue=8e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 55483; Mature: 55483

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLVNLVNGIIWSPALIYLCLGAGLFYSIMTRFVQVRLFGEMVRLLFTGKSSADGISSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QALAVALAGRVGMGNIAGVAAAIGFGGPGAVFWMWVVAFLGASTAYVESTLAQIYKERDV
HHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
ITGEYRGGPAYYFERALGQKWYGILFAIASILACGMFLPGVQANGVLSAFTQLMGEGTTM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
NVAGLEVGSMRLIGLAIILVVLGIIIFGGIKRIATFTEYAVPFMAVGYIVLALIIMFSNF
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLIPDIFGLIISDAFTAQAGFGAAIGWGVKRGIYSNEAGQGTGPHAAAAAEVQHPSQQGL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHH
VQAFSVYVDTLLVCSATAFMILSTGMYNIQGTLPEGQFILQNVAATTEINSPAFTQMAME
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
SVYGTTGNVFIAVAVFFFAFTTILAYYYIAEVNVAYLTRFIGRGANKTGLFLVKILIMVM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAYGALNSAGYIWAIGDIGVGLMAWLNIVGILVIFFVARPTLTMLKDYEAQLKAGVTRYT
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHEE
FDPAKFGIKNAPYWEERHLKEQQRIAAQDPLNKEHR
CCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEGLVNLVNGIIWSPALIYLCLGAGLFYSIMTRFVQVRLFGEMVRLLFTGKSSADGISSF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QALAVALAGRVGMGNIAGVAAAIGFGGPGAVFWMWVVAFLGASTAYVESTLAQIYKERDV
HHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
ITGEYRGGPAYYFERALGQKWYGILFAIASILACGMFLPGVQANGVLSAFTQLMGEGTTM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
NVAGLEVGSMRLIGLAIILVVLGIIIFGGIKRIATFTEYAVPFMAVGYIVLALIIMFSNF
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLIPDIFGLIISDAFTAQAGFGAAIGWGVKRGIYSNEAGQGTGPHAAAAAEVQHPSQQGL
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHH
VQAFSVYVDTLLVCSATAFMILSTGMYNIQGTLPEGQFILQNVAATTEINSPAFTQMAME
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
SVYGTTGNVFIAVAVFFFAFTTILAYYYIAEVNVAYLTRFIGRGANKTGLFLVKILIMVM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VAYGALNSAGYIWAIGDIGVGLMAWLNIVGILVIFFVARPTLTMLKDYEAQLKAGVTRYT
HHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHEE
FDPAKFGIKNAPYWEERHLKEQQRIAAQDPLNKEHR
CCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]