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The map label for this gene is lip [H]

Identifier: 93005273

GI number: 93005273

Start: 543879

End: 544934

Strand: Direct

Name: lip [H]

Synonym: Pcryo_0443

Alternate gene names: 93005273

Gene position: 543879-544934 (Clockwise)

Preceding gene: 93005270

Following gene: 93005274

Centisome position: 17.77

GC content: 45.83

Gene sequence:

>1056_bases
ATGACTCTAAAACCTTCTCTGCGCGCTGTGCTCACGCCGAGCGCATTTTTACCGTCATTAGGTGCCATTGTCACCGGTGC
GCTTTTGATGACTATGCAAGCCACCTCTGCTCAGGCGGCTGGACAGTATTATAACTGCGCCAATGCTGACGGCTGCCAGC
TCGTAAGTAGTCAATATTTCACCTCAGATCATACCAAAACCAAATACCCAGTAGTACTCGCACATGGTATGGCAGGGTTT
ACTAAGATGTTCGGCTTGGTTGATTATTTTTATGGTATTCCTGAAACACTCATGAGCGGTGGCAGTCAAGTATATACCAC
AAAAACCTCCTCTTTTAACAATAGCGAAATTCGTGGCGAGCAGCTTTTACAGAAAATAAAAACCATCAGTGCCATTTCAG
GCAGCCCAAAAGTGAACTTATTGGGTCATAGCCAAGGTGGTATTGATATTCGTTATGTCGCTGGCGTGGCACCAGAATAT
GTTGCTTCTGTCACTGCGATTGGCAGTCCAGAGCAAGGGTCAAAAACGGCGGATGCTGTTATGCAAACACTTTCTGAGGA
TAGCTTGGCTACCCAAGCGGTATCTGTGTTCTTTCAGCTATTCGGTGGCGTTACGGATGTCGGTTCAGGTACGAGCCCGA
CAGAACTCAATAAACAAGAAGCTTGGAATACAGCTGTCGCCTTATCAACTGATGGTGCTGCCAAGTTTAATGCTAAATTT
CCTGCGGCGATGCCGACCAGCTATTGTGGACAGCCTACTGGCACTGAAGCCAATGGTATCAAGTACTACTCATTTAGTGG
TGTTGGGCAAATAACCAATGGTCTTGATCCTAGTGATTATATGCTCGCGCTAACAGGAACAAGTTTTGACAATGATCCAA
ATGATGGCTTGGTATCTGCGTGCTCAAGCCGACTGGGTTATGTGATTCGAGATGATTATCGTATGAATCACTTAGATTCA
GCCAACCAGCTTTTGGGTTTGACCGCATGGGGGCAGCCTGACCCAAAAACCTTGTATCGTGATCAAGTCAATCGCTTGAA
AAAAGCTAATTTATAA

Upstream 100 bases:

>100_bases
CGCTAAATGATTTAGCAGACCTCTTTATAGCTTTGTGCAGGATGCAAGAGATAAGGCAATATAAAGATAGAATCACGTTT
ACAATAACAAGGATGTTGTT

Downstream 100 bases:

>100_bases
CGTTCATAGACGAGTTAGCAGATTCACAGCTTTTATGATTCATAGCTTTTATAGAAAAAGGGTCAATACTAAAATGACCC
TTTTTCTTTATCCATTTTTA

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTLKPSLRAVLTPSAFLPSLGAIVTGALLMTMQATSAQAAGQYYNCANADGCQLVSSQYFTSDHTKTKYPVVLAHGMAGF
TKMFGLVDYFYGIPETLMSGGSQVYTTKTSSFNNSEIRGEQLLQKIKTISAISGSPKVNLLGHSQGGIDIRYVAGVAPEY
VASVTAIGSPEQGSKTADAVMQTLSEDSLATQAVSVFFQLFGGVTDVGSGTSPTELNKQEAWNTAVALSTDGAAKFNAKF
PAAMPTSYCGQPTGTEANGIKYYSFSGVGQITNGLDPSDYMLALTGTSFDNDPNDGLVSACSSRLGYVIRDDYRMNHLDS
ANQLLGLTAWGQPDPKTLYRDQVNRLKKANL

Sequences:

>Translated_351_residues
MTLKPSLRAVLTPSAFLPSLGAIVTGALLMTMQATSAQAAGQYYNCANADGCQLVSSQYFTSDHTKTKYPVVLAHGMAGF
TKMFGLVDYFYGIPETLMSGGSQVYTTKTSSFNNSEIRGEQLLQKIKTISAISGSPKVNLLGHSQGGIDIRYVAGVAPEY
VASVTAIGSPEQGSKTADAVMQTLSEDSLATQAVSVFFQLFGGVTDVGSGTSPTELNKQEAWNTAVALSTDGAAKFNAKF
PAAMPTSYCGQPTGTEANGIKYYSFSGVGQITNGLDPSDYMLALTGTSFDNDPNDGLVSACSSRLGYVIRDDYRMNHLDS
ANQLLGLTAWGQPDPKTLYRDQVNRLKKANL
>Mature_350_residues
TLKPSLRAVLTPSAFLPSLGAIVTGALLMTMQATSAQAAGQYYNCANADGCQLVSSQYFTSDHTKTKYPVVLAHGMAGFT
KMFGLVDYFYGIPETLMSGGSQVYTTKTSSFNNSEIRGEQLLQKIKTISAISGSPKVNLLGHSQGGIDIRYVAGVAPEYV
ASVTAIGSPEQGSKTADAVMQTLSEDSLATQAVSVFFQLFGGVTDVGSGTSPTELNKQEAWNTAVALSTDGAAKFNAKFP
AAMPTSYCGQPTGTEANGIKYYSFSGVGQITNGLDPSDYMLALTGTSFDNDPNDGLVSACSSRLGYVIRDDYRMNHLDSA
NQLLGLTAWGQPDPKTLYRDQVNRLKKANL

Specific function: Catalyzes the hydrolysis of triglycerides. Catalyzes the synthesis of macrocyclic lactones in anhydrous organic solvents [H]

COG id: COG1075

COG function: function code R; Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold

Gene ontology:

Cell location: Cell surface. Secreted, extracellular space. Note=During early stationary growth phase about 10% of the enzyme molecules remain cell-bound while about 90% are released into the growth medium [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Pseudomonas lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 37218; Mature: 37087

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKPSLRAVLTPSAFLPSLGAIVTGALLMTMQATSAQAAGQYYNCANADGCQLVSSQYF
CCCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
TSDHTKTKYPVVLAHGMAGFTKMFGLVDYFYGIPETLMSGGSQVYTTKTSSFNNSEIRGE
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCCHH
QLLQKIKTISAISGSPKVNLLGHSQGGIDIRYVAGVAPEYVASVTAIGSPEQGSKTADAV
HHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
MQTLSEDSLATQAVSVFFQLFGGVTDVGSGTSPTELNKQEAWNTAVALSTDGAAKFNAKF
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHCCEEEEEECCCCCEECCCC
PAAMPTSYCGQPTGTEANGIKYYSFSGVGQITNGLDPSDYMLALTGTSFDNDPNDGLVSA
CCCCCHHHCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
CSSRLGYVIRDDYRMNHLDSANQLLGLTAWGQPDPKTLYRDQVNRLKKANL
HHHHCCEEEECCCCCCCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLKPSLRAVLTPSAFLPSLGAIVTGALLMTMQATSAQAAGQYYNCANADGCQLVSSQYF
CCCCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHC
TSDHTKTKYPVVLAHGMAGFTKMFGLVDYFYGIPETLMSGGSQVYTTKTSSFNNSEIRGE
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCCHH
QLLQKIKTISAISGSPKVNLLGHSQGGIDIRYVAGVAPEYVASVTAIGSPEQGSKTADAV
HHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHH
MQTLSEDSLATQAVSVFFQLFGGVTDVGSGTSPTELNKQEAWNTAVALSTDGAAKFNAKF
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCHHHHCCEEEEEECCCCCEECCCC
PAAMPTSYCGQPTGTEANGIKYYSFSGVGQITNGLDPSDYMLALTGTSFDNDPNDGLVSA
CCCCCHHHCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHH
CSSRLGYVIRDDYRMNHLDSANQLLGLTAWGQPDPKTLYRDQVNRLKKANL
HHHHCCEEEECCCCCCCCCCHHHEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1512563; 1632642; 1576157; 10984043; 1748875; 11133953 [H]