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The map label for this gene is rlmH [H]

Identifier: 93005263

GI number: 93005263

Start: 530596

End: 531096

Strand: Reverse

Name: rlmH [H]

Synonym: Pcryo_0433

Alternate gene names: 93005263

Gene position: 531096-530596 (Counterclockwise)

Preceding gene: 93005264

Following gene: 93005262

Centisome position: 17.36

GC content: 45.91

Gene sequence:

>501_bases
ATGGCAGCTGTTATGAAAGTAAGAATTTTGACCGTAGGTAATAAAATGCCCAAGTGGGTACAAACTGGTTTCGATGAATA
TCATAAGCGTATTCAGCCTATGCTCACTACCGAAGTCGTTGAAATTGCGGCGGCAAAACGTGCTAAGAATCCATCAGATG
CCAATTTAGCTCAATACCGTGAGCAAGAAGGTCAGGCGATATTAGCCGCTCATGCTGTAGCAGGACGTGAGCAATTGTGG
GTACTTGATGTCAAGGGTAAGATGCTATCGACCGAAAATTTGGCAGATAAATTGTCTGATGGTATGCAGCAAGGCGATGA
TATCGCTTTGGTCATCGGTGGTGCCGATGGTGTGTCACCAGAGGTATTGGCAAAAGCAGATGTGAAATGGTCGTTATCGG
CGCTTACGCTACCGCATCCTTTAGTGCGCGTTGTCCTCATGGAGCAGCTATATCGCGCGATGAGTATCAATCATAACCAC
CCTTATCACCGTGGTAATTAA

Upstream 100 bases:

>100_bases
ATTAAAATCATACAGAATAATTATTTATCAAATACGTCTTGAGCAAAGAATCTAGTATTTAACAGGTTTTCTTATTACAT
TTATTTAACCACTTCTTAAA

Downstream 100 bases:

>100_bases
AGTAGCAATAGAAAACTATCCCGTAGGAAAACATCATCAATCACAGTGACACAACAGATTGAAAAATGGACGCACGCCCT
AATAAATGCCACATGAGACA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MAAVMKVRILTVGNKMPKWVQTGFDEYHKRIQPMLTTEVVEIAAAKRAKNPSDANLAQYREQEGQAILAAHAVAGREQLW
VLDVKGKMLSTENLADKLSDGMQQGDDIALVIGGADGVSPEVLAKADVKWSLSALTLPHPLVRVVLMEQLYRAMSINHNH
PYHRGN

Sequences:

>Translated_166_residues
MAAVMKVRILTVGNKMPKWVQTGFDEYHKRIQPMLTTEVVEIAAAKRAKNPSDANLAQYREQEGQAILAAHAVAGREQLW
VLDVKGKMLSTENLADKLSDGMQQGDDIALVIGGADGVSPEVLAKADVKWSLSALTLPHPLVRVVLMEQLYRAMSINHNH
PYHRGN
>Mature_165_residues
AAVMKVRILTVGNKMPKWVQTGFDEYHKRIQPMLTTEVVEIAAAKRAKNPSDANLAQYREQEGQAILAAHAVAGREQLWV
LDVKGKMLSTENLADKLSDGMQQGDDIALVIGGADGVSPEVLAKADVKWSLSALTLPHPLVRVVLMEQLYRAMSINHNHP
YHRGN

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=160, Percent_Identity=49.375, Blast_Score=153, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 18305; Mature: 18174

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVMKVRILTVGNKMPKWVQTGFDEYHKRIQPMLTTEVVEIAAAKRAKNPSDANLAQYR
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
EQEGQAILAAHAVAGREQLWVLDVKGKMLSTENLADKLSDGMQQGDDIALVIGGADGVSP
HHCCCEEEEHHHHCCCCEEEEEECCCCEECHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EVLAKADVKWSLSALTLPHPLVRVVLMEQLYRAMSINHNHPYHRGN
HHHHHCCCCEEEHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AAVMKVRILTVGNKMPKWVQTGFDEYHKRIQPMLTTEVVEIAAAKRAKNPSDANLAQYR
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
EQEGQAILAAHAVAGREQLWVLDVKGKMLSTENLADKLSDGMQQGDDIALVIGGADGVSP
HHCCCEEEEHHHHCCCCEEEEEECCCCEECHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
EVLAKADVKWSLSALTLPHPLVRVVLMEQLYRAMSINHNHPYHRGN
HHHHHCCCCEEEHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA