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The map label for this gene is aat

Identifier: 93005258

GI number: 93005258

Start: 521903

End: 522727

Strand: Reverse

Name: aat

Synonym: Pcryo_0428

Alternate gene names: 93005258

Gene position: 522727-521903 (Counterclockwise)

Preceding gene: 93005259

Following gene: 93005257

Centisome position: 17.08

GC content: 43.27

Gene sequence:

>825_bases
ATGAGCGATTTTGTTAACGATGGTCATACAACGCCTGAGGCTTTTGTTCAACATATCAAAAGTCTTGGGCGTTATGACTT
TCCAGAGCCAGCGATAGTAGATCCTGAAGGTATGGGTATTGTGGCTATCGGGGGAGATTTAGCTCCTGAGACGCTGATTT
CTGCTTACGCACAGGGTCTATTTCCTTGGTTTAATGACGATGAGCCAATTGCTTGGTGGTGCCCTGAACCCAGATGTGTG
ATGCAGCCGACAGACTATCAGCCCAGTAAATCGCTACGTAAGCTAGCAAACCGTAGTCGTTGGCAGTTGACACTGAATCA
AGCTTTTGATGAAGTCATACACGCTTGTAGTTTACCGCGTAGTAATGGCGTTAATAACGATCTTAATGAAAGTCAGCCTG
TTGGCGAACATACTTGGATTCATGATGAGATGATTGAAGCTTATACTGAGCTACATGCTCAAGGCTTCGCGCATAGTATT
GAAGTTTGGGATGATAAAGGCGCGCTCGTTGGTGGTTTATATGGCTTAAAAATAGGCGGTATTTATTTTGGTGAATCGAT
GTTCCATATCGCTTCTAATGCATCAAAGCTAGCATTTTGGGGCTTGATGCGCTTATGTGAGCACAGCAATGTTAATCTTG
TCGACTGTCAACTCCCTAACGATCATCTCATGAGTTTAGGTGCTATCACCTTGTCACGTACTGAGTTTTTGACTCAACTA
GATATATTAATATCTAATAGTTCAGATAATTGGCACAAAAACTCGCATAAGCCTCTAGCGGTACCGCTGTTAGGGAGTTT
GCAACCTTGGCAATTAAACCTCTAG

Upstream 100 bases:

>100_bases
GGCAGCACTTGATGCTGAAAAGTACCTAGATGCAATAGGCGAAACGGTCGCTCAAGATCATACTTATGCTTCAACGTTGA
CAGCTGACAAAGAAGCGTAA

Downstream 100 bases:

>100_bases
TTTATTACTTTATTTTAAAATCTAAAGTTGATCTGGCTAATCGATACATGAAATAGCGCGACTCGATTGAGTACGTATGA
TTATTACTATTACGTTTAAT

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSDFVNDGHTTPEAFVQHIKSLGRYDFPEPAIVDPEGMGIVAIGGDLAPETLISAYAQGLFPWFNDDEPIAWWCPEPRCV
MQPTDYQPSKSLRKLANRSRWQLTLNQAFDEVIHACSLPRSNGVNNDLNESQPVGEHTWIHDEMIEAYTELHAQGFAHSI
EVWDDKGALVGGLYGLKIGGIYFGESMFHIASNASKLAFWGLMRLCEHSNVNLVDCQLPNDHLMSLGAITLSRTEFLTQL
DILISNSSDNWHKNSHKPLAVPLLGSLQPWQLNL

Sequences:

>Translated_274_residues
MSDFVNDGHTTPEAFVQHIKSLGRYDFPEPAIVDPEGMGIVAIGGDLAPETLISAYAQGLFPWFNDDEPIAWWCPEPRCV
MQPTDYQPSKSLRKLANRSRWQLTLNQAFDEVIHACSLPRSNGVNNDLNESQPVGEHTWIHDEMIEAYTELHAQGFAHSI
EVWDDKGALVGGLYGLKIGGIYFGESMFHIASNASKLAFWGLMRLCEHSNVNLVDCQLPNDHLMSLGAITLSRTEFLTQL
DILISNSSDNWHKNSHKPLAVPLLGSLQPWQLNL
>Mature_273_residues
SDFVNDGHTTPEAFVQHIKSLGRYDFPEPAIVDPEGMGIVAIGGDLAPETLISAYAQGLFPWFNDDEPIAWWCPEPRCVM
QPTDYQPSKSLRKLANRSRWQLTLNQAFDEVIHACSLPRSNGVNNDLNESQPVGEHTWIHDEMIEAYTELHAQGFAHSIE
VWDDKGALVGGLYGLKIGGIYFGESMFHIASNASKLAFWGLMRLCEHSNVNLVDCQLPNDHLMSLGAITLSRTEFLTQLD
ILISNSSDNWHKNSHKPLAVPLLGSLQPWQLNL

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=218, Percent_Identity=41.743119266055, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_PSYCK (Q1QDP2)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579695.1
- ProteinModelPortal:   Q1QDP2
- SMR:   Q1QDP2
- STRING:   Q1QDP2
- GeneID:   4034227
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0428
- NMPDR:   fig|335284.3.peg.1175
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- PhylomeDB:   Q1QDP2
- ProtClustDB:   PRK00301
- BioCyc:   PCRY335284:PCRYO_0428-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 30622; Mature: 30491

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFVNDGHTTPEAFVQHIKSLGRYDFPEPAIVDPEGMGIVAIGGDLAPETLISAYAQGL
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHC
FPWFNDDEPIAWWCPEPRCVMQPTDYQPSKSLRKLANRSRWQLTLNQAFDEVIHACSLPR
CCCCCCCCCEEEECCCCCEECCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCC
SNGVNNDLNESQPVGEHTWIHDEMIEAYTELHAQGFAHSIEVWDDKGALVGGLYGLKIGG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEHEEECC
IYFGESMFHIASNASKLAFWGLMRLCEHSNVNLVDCQLPNDHLMSLGAITLSRTEFLTQL
EEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHE
DILISNSSDNWHKNSHKPLAVPLLGSLQPWQLNL
EEEEECCCCCCCCCCCCCEEEECCCCCCCEEECC
>Mature Secondary Structure 
SDFVNDGHTTPEAFVQHIKSLGRYDFPEPAIVDPEGMGIVAIGGDLAPETLISAYAQGL
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHC
FPWFNDDEPIAWWCPEPRCVMQPTDYQPSKSLRKLANRSRWQLTLNQAFDEVIHACSLPR
CCCCCCCCCEEEECCCCCEECCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCC
SNGVNNDLNESQPVGEHTWIHDEMIEAYTELHAQGFAHSIEVWDDKGALVGGLYGLKIGG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEHEEECC
IYFGESMFHIASNASKLAFWGLMRLCEHSNVNLVDCQLPNDHLMSLGAITLSRTEFLTQL
EEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHE
DILISNSSDNWHKNSHKPLAVPLLGSLQPWQLNL
EEEEECCCCCCCCCCCCCEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA