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The map label for this gene is tuf1 [H]

Identifier: 93005255

GI number: 93005255

Start: 519410

End: 520600

Strand: Direct

Name: tuf1 [H]

Synonym: Pcryo_0425

Alternate gene names: 93005255

Gene position: 519410-520600 (Clockwise)

Preceding gene: 93005254

Following gene: 93005256

Centisome position: 16.97

GC content: 48.11

Gene sequence:

>1191_bases
ATGGCAAAGGCCAAGTTTGAACGTCTAAAACCACATGTTAACGTCGGTACCATCGGACACGTTGACCACGGTAAAACCAC
CCTTACCGCTGCAATCGCAACCGTAGCTGCAATCACCTCTGGTGGCGAAGCCAAAGACTACGCGTCTATTGACTCAGCAC
CAGAAGAAAAAGCACGTGGCATCACCATCAACACCTCACACGTAGAATATGACACCCCATCACGTCATTACGCTCACGTT
GATTGCCCAGGTCACGCCGATTATGTTAAAAACATGATCACCGGTGCAGCCCAGATGGACGGCGCAATCCTAGTAGTATC
AGCAACTGACGGCCCTATGCCACAAACCCGTGAGCACATCCTGCTTTCACGTCAGGTTGGCGTACCGTACATCGTCGTAT
TCATGAACAAGTGCGACGTTGTTGATGACGAAGAATTGCTTGAACTCGTAGAAATGGAAGTTCGTGAATTATTAAGCGAC
TATGACTTCCCAGGTGATGACACCCCAATCATCCATGGTTCTGCCACTGAAGCCCTAAAAGGCTCACAAGAAAAATATGG
TCAACCAGCCGTTGTAGAACTATTAAACGTTCTTGACACCTACATCCCAGAGCCAGAGCGTGACATCGACAAAGCATTCC
TAATGCCAATCGAAGACGTATTCTCAATCTCAGGTCGTGGTACTGTTGTAACTGGCCGTGTTGAATCTGGTATCGTACGC
GTTGGCGACGAAATCGAAATCGTTGGTATCCGTGACACTCAAAAAACCACCTGTACTGGTGTAGAGATGTTCCGTAAACT
GCTTGACGAAGGTCGTGCAGGCGAAAACTGTGGCGTACTACTACGTGGTACTAAGCGTGAAGACGTACAACGTGGCCAAG
TACTAGCTAAGCCAGGTTCAATCACTCCTCACACCAAGTTTGACGCTGAAGTATATGTATTGTCAAAAGAAGAGGGTGGT
CGTCACACACCATTCCTAAACGGCTATCGTCCACAGTTCTATTTCCGTACCACTGACGTAACTGGCGCAATCCAATTACA
AGACGGTACTGAAATGGTTATGCCTGGTGATAACGTTGAGATGGGCGTAGAGCTTATCCACCCAATCGCTATGGACAAAG
GTCTTCGCTTTGCTATTCGTGAAGGTGGCCGTACGGTAGGCGCTGGTGTTGTTGCTAACGTATTGAACTAA

Upstream 100 bases:

>100_bases
CACAGTGTCAACGGTTTGATGGTCATACATCATAAGCCGTCAAACAGTTATTTACCGATCTTTGATTGATAAATATTCAC
CAAAAAAAGAGGAAATACCC

Downstream 100 bases:

>100_bases
TACTTAACGCTGTCAGAGTAATCTGATCTTCGTTAATAATTAGTCAAAAGCCATCCTCGAAAGAGGGTGGCTTTTTTTGG
GTATGGTTTGCTAATAGAAT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERLKPHVNVGTIGHVDHGKTTLTAAIATVAAITSGGEAKDYASIDSAPEEKARGITINTSHVEYDTPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHILLSRQVGVPYIVVFMNKCDVVDDEELLELVEMEVRELLSD
YDFPGDDTPIIHGSATEALKGSQEKYGQPAVVELLNVLDTYIPEPERDIDKAFLMPIEDVFSISGRGTVVTGRVESGIVR
VGDEIEIVGIRDTQKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGSITPHTKFDAEVYVLSKEEGG
RHTPFLNGYRPQFYFRTTDVTGAIQLQDGTEMVMPGDNVEMGVELIHPIAMDKGLRFAIREGGRTVGAGVVANVLN

Sequences:

>Translated_396_residues
MAKAKFERLKPHVNVGTIGHVDHGKTTLTAAIATVAAITSGGEAKDYASIDSAPEEKARGITINTSHVEYDTPSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHILLSRQVGVPYIVVFMNKCDVVDDEELLELVEMEVRELLSD
YDFPGDDTPIIHGSATEALKGSQEKYGQPAVVELLNVLDTYIPEPERDIDKAFLMPIEDVFSISGRGTVVTGRVESGIVR
VGDEIEIVGIRDTQKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGSITPHTKFDAEVYVLSKEEGG
RHTPFLNGYRPQFYFRTTDVTGAIQLQDGTEMVMPGDNVEMGVELIHPIAMDKGLRFAIREGGRTVGAGVVANVLN
>Mature_395_residues
AKAKFERLKPHVNVGTIGHVDHGKTTLTAAIATVAAITSGGEAKDYASIDSAPEEKARGITINTSHVEYDTPSRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHILLSRQVGVPYIVVFMNKCDVVDDEELLELVEMEVRELLSDY
DFPGDDTPIIHGSATEALKGSQEKYGQPAVVELLNVLDTYIPEPERDIDKAFLMPIEDVFSISGRGTVVTGRVESGIVRV
GDEIEIVGIRDTQKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGSITPHTKFDAEVYVLSKEEGGR
HTPFLNGYRPQFYFRTTDVTGAIQLQDGTEMVMPGDNVEMGVELIHPIAMDKGLRFAIREGGRTVGAGVVANVLN

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.3451776649746, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=440, Percent_Identity=29.3181818181818, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.185520361991, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=28.6681715575621, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=28.6681715575621, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=28.4345047923323, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI194018522, Length=341, Percent_Identity=28.7390029325513, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018520, Length=341, Percent_Identity=28.7390029325513, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194097354, Length=337, Percent_Identity=29.080118694362, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI46094014, Length=341, Percent_Identity=28.7390029325513, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI4503507, Length=365, Percent_Identity=28.2191780821918, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI45593140, Length=429, Percent_Identity=24.009324009324, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=35.6060606060606, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=36.697247706422, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=36.697247706422, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=36.697247706422, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=30.9623430962343, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=30.9623430962343, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=80.8080808080808, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=81.0126582278481, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=387, Percent_Identity=29.7157622739018, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI48994988, Length=237, Percent_Identity=30.379746835443, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789108, Length=318, Percent_Identity=28.9308176100629, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=32.8767123287671, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788922, Length=255, Percent_Identity=28.6274509803922, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=53.3834586466165, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.9276485788114, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI17552884, Length=442, Percent_Identity=28.5067873303167, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17569207, Length=442, Percent_Identity=28.5067873303167, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=27.816091954023, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=27.7777777777778, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=26.1958997722096, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=27.1889400921659, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=28.9377289377289, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17509919, Length=271, Percent_Identity=28.0442804428044, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=35.8620689655172, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=26.6094420600858, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.727959697733, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=438, Percent_Identity=28.5388127853881, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=438, Percent_Identity=28.5388127853881, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=360, Percent_Identity=26.1111111111111, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=342, Percent_Identity=29.5321637426901, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=268, Percent_Identity=29.1044776119403, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=27.1604938271605, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=55.7251908396947, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=55.7251908396947, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=49.6103896103896, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24652838, Length=446, Percent_Identity=27.5784753363229, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17137572, Length=446, Percent_Identity=27.5784753363229, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45553807, Length=446, Percent_Identity=27.3542600896861, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI45553816, Length=446, Percent_Identity=27.3542600896861, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24651721, Length=446, Percent_Identity=27.3542600896861, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI17864154, Length=446, Percent_Identity=27.3542600896861, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=29.9342105263158, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI45550900, Length=439, Percent_Identity=27.7904328018223, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI17137380, Length=340, Percent_Identity=30.2941176470588, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.2527881040892, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.2527881040892, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28572034, Length=179, Percent_Identity=35.195530726257, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43076; Mature: 42945

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERLKPHVNVGTIGHVDHGKTTLTAAIATVAAITSGGEAKDYASIDSAPEEKARG
CCCCCHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITINTSHVEYDTPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHI
EEEECCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKCDVVDDEELLELVEMEVRELLSDYDFPGDDTPIIHGSATEALK
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHC
GSQEKYGQPAVVELLNVLDTYIPEPERDIDKAFLMPIEDVFSISGRGTVVTGRVESGIVR
CCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHCCCCCCEEEECCCCCCEEE
VGDEIEIVGIRDTQKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGS
ECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCEEECCCC
ITPHTKFDAEVYVLSKEEGGRHTPFLNGYRPQFYFRTTDVTGAIQLQDGTEMVMPGDNVE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCHH
MGVELIHPIAMDKGLRFAIREGGRTVGAGVVANVLN
HHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHCC
>Mature Secondary Structure 
AKAKFERLKPHVNVGTIGHVDHGKTTLTAAIATVAAITSGGEAKDYASIDSAPEEKARG
CCCCHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITINTSHVEYDTPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSATDGPMPQTREHI
EEEECCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKCDVVDDEELLELVEMEVRELLSDYDFPGDDTPIIHGSATEALK
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHC
GSQEKYGQPAVVELLNVLDTYIPEPERDIDKAFLMPIEDVFSISGRGTVVTGRVESGIVR
CCHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHCCCCCCEEEECCCCCCEEE
VGDEIEIVGIRDTQKTTCTGVEMFRKLLDEGRAGENCGVLLRGTKREDVQRGQVLAKPGS
ECCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCEEECCCC
ITPHTKFDAEVYVLSKEEGGRHTPFLNGYRPQFYFRTTDVTGAIQLQDGTEMVMPGDNVE
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCHH
MGVELIHPIAMDKGLRFAIREGGRTVGAGVVANVLN
HHHHHHHHHHHCCCCEEEECCCCCEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA