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The map label for this gene is prfC [H]

Identifier: 93005252

GI number: 93005252

Start: 514002

End: 515597

Strand: Direct

Name: prfC [H]

Synonym: Pcryo_0422

Alternate gene names: 93005252

Gene position: 514002-515597 (Clockwise)

Preceding gene: 93005245

Following gene: 93005253

Centisome position: 16.8

GC content: 46.49

Gene sequence:

>1596_bases
ATGAGCGTAGATCCAAAGAAATTAAATAAAGAAGTGGCCAAGCGCCGCACTTTTGCCATCATCTCGCATCCTGATGCTGG
TAAGACGACGATGACCGAAAAGCTGCTGCTATGGGGTAAAGCGATTCAAGTAGTGGGCGAGGTCAAAGGTCGTAAAACCG
ACCGCCATGCGACATCTGATTGGATGAGTATGGAGCAGGAGCGTGGTATTTCTATTACCACTTCGGTCATGCAGTTCCCT
TATAAAGAACATATGGTCAACCTGCTAGATACACCGGGTCACGCTGACTTTAGTGAAGATACCTATCGTACTCTGACCGC
TGTAGACAGTGCGCTGATGATGGTCGATGGCGCAAAAGGTGTCGAAGAACGTACCATCAAATTGATGGAAGTTTGCCGGA
TGCGTGATACACCCATCATCTCATTCGTCAATAAACTTGACCGCCAGATTCGTGAGCCGCTTGAGCTATTGAGTGAAATC
GAAGCGGTATTAAAGATTAAATGTATTCCTGTCACTTGGCCTATCGGTATGGGACAGGATTTTGTTGGTGTCTATCATTT
AACCGAAAACAAAACCTATTTTTATGAGAAAGGTCATGGCGGTGATATGACGGTCGCTGAGACGCGCGAAGGCTATGATT
ATCCTGATATCCGTGAGCGCTTGGGCGCTTTGATGTTCGCCTCATTTGAAGAGTCATTAGAATTGGTACAAATGGCGCTA
GAAGATTTCGATGTTGACGAGTTTTTAGCCGGTGAGATGACACCCGTATTGTTCGGTACAGCGCTAGGTAACTTTGGCGT
CAATATGGTACTTGATACTTTAATCAAATATGCGCCGCCACCAAAAGCGCATCCGACCAATGAACGTGAAGTAGCGGCGA
CTGAGACAACGTTTAGTGGTTTTGTCTTTAAGATTCAGGCAAACATGGATCCGCGTCATCGTGATCGCATTGCATTTTTA
CGTGTCTGCTCAGGTAAGTACGAAAAAGGTATGAAATTAAAGCATGTACGCTTGGGTAAAGACGTACGTATTGCTGATGC
TTTGACCTTTTTAGCAGGTGACCGCGAAGCGTTAGAAGAAGCCTATCCGGGTGATATTATTGGTTTACACAATCATGGCA
CCATCTCTATCGGCGATAGTTTCACTGAAGGCGAAGAGCTCAACTTTACCGGTATCCCGCATTTCGCCCCTGAGCTATTC
CGTCGTGTCATTCTAAAAGATCCGCTCAAATCAAAAGCGCTGCAAAAAGGTCTACAGCAGTTGAGTGAAGAGGGCGCAAC
GCAGGTCTTTATGCCGCAGATTAATAATGATTTAATCTTGGGTGCGGTCGGTGTACTACAGTTCGAAGTGGTCGCCCATC
GTCTCAAAGAAGAGTACAAAGTACAGTGTATCTTTGAGCCGGTATCGATTGCGACCGTTCGCTGGATTCATTGCGATGAC
GAAGTGGCGTTAGCCAAGTTTAAGCGCAAAGCCCATGACCAACTATCGCTAGATGGCGGTGGTCATCTGACTTATCTTGC
GCCATCACGGGTGAACTTGCAGCTGATGCAAGATCGCTATCCAGAAGTGACGTTTAGCAATACGCGTGAGCACTAA

Upstream 100 bases:

>100_bases
ATCCGCTACAATACGCGCACCAAAATCCATCACATTTACTGTTAGACCTTTTCTAAATTCTTGTTTTTTTCTTCCAAAAT
TTAACGATAAGTTATTCATT

Downstream 100 bases:

>100_bases
AATCCATTATGCCACTTTGCTTAATCTTAAAAGACACGTTATCGTGTCTTTTTTATTAGCTTATCAAACAGCGTATTATT
GATTTTAATCCCTATGGTTC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MSVDPKKLNKEVAKRRTFAIISHPDAGKTTMTEKLLLWGKAIQVVGEVKGRKTDRHATSDWMSMEQERGISITTSVMQFP
YKEHMVNLLDTPGHADFSEDTYRTLTAVDSALMMVDGAKGVEERTIKLMEVCRMRDTPIISFVNKLDRQIREPLELLSEI
EAVLKIKCIPVTWPIGMGQDFVGVYHLTENKTYFYEKGHGGDMTVAETREGYDYPDIRERLGALMFASFEESLELVQMAL
EDFDVDEFLAGEMTPVLFGTALGNFGVNMVLDTLIKYAPPPKAHPTNEREVAATETTFSGFVFKIQANMDPRHRDRIAFL
RVCSGKYEKGMKLKHVRLGKDVRIADALTFLAGDREALEEAYPGDIIGLHNHGTISIGDSFTEGEELNFTGIPHFAPELF
RRVILKDPLKSKALQKGLQQLSEEGATQVFMPQINNDLILGAVGVLQFEVVAHRLKEEYKVQCIFEPVSIATVRWIHCDD
EVALAKFKRKAHDQLSLDGGGHLTYLAPSRVNLQLMQDRYPEVTFSNTREH

Sequences:

>Translated_531_residues
MSVDPKKLNKEVAKRRTFAIISHPDAGKTTMTEKLLLWGKAIQVVGEVKGRKTDRHATSDWMSMEQERGISITTSVMQFP
YKEHMVNLLDTPGHADFSEDTYRTLTAVDSALMMVDGAKGVEERTIKLMEVCRMRDTPIISFVNKLDRQIREPLELLSEI
EAVLKIKCIPVTWPIGMGQDFVGVYHLTENKTYFYEKGHGGDMTVAETREGYDYPDIRERLGALMFASFEESLELVQMAL
EDFDVDEFLAGEMTPVLFGTALGNFGVNMVLDTLIKYAPPPKAHPTNEREVAATETTFSGFVFKIQANMDPRHRDRIAFL
RVCSGKYEKGMKLKHVRLGKDVRIADALTFLAGDREALEEAYPGDIIGLHNHGTISIGDSFTEGEELNFTGIPHFAPELF
RRVILKDPLKSKALQKGLQQLSEEGATQVFMPQINNDLILGAVGVLQFEVVAHRLKEEYKVQCIFEPVSIATVRWIHCDD
EVALAKFKRKAHDQLSLDGGGHLTYLAPSRVNLQLMQDRYPEVTFSNTREH
>Mature_530_residues
SVDPKKLNKEVAKRRTFAIISHPDAGKTTMTEKLLLWGKAIQVVGEVKGRKTDRHATSDWMSMEQERGISITTSVMQFPY
KEHMVNLLDTPGHADFSEDTYRTLTAVDSALMMVDGAKGVEERTIKLMEVCRMRDTPIISFVNKLDRQIREPLELLSEIE
AVLKIKCIPVTWPIGMGQDFVGVYHLTENKTYFYEKGHGGDMTVAETREGYDYPDIRERLGALMFASFEESLELVQMALE
DFDVDEFLAGEMTPVLFGTALGNFGVNMVLDTLIKYAPPPKAHPTNEREVAATETTFSGFVFKIQANMDPRHRDRIAFLR
VCSGKYEKGMKLKHVRLGKDVRIADALTFLAGDREALEEAYPGDIIGLHNHGTISIGDSFTEGEELNFTGIPHFAPELFR
RVILKDPLKSKALQKGLQQLSEEGATQVFMPQINNDLILGAVGVLQFEVVAHRLKEEYKVQCIFEPVSIATVRWIHCDDE
VALAKFKRKAHDQLSLDGGGHLTYLAPSRVNLQLMQDRYPEVTFSNTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=483, Percent_Identity=26.7080745341615, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI19923640, Length=517, Percent_Identity=25.3384912959381, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=401, Percent_Identity=27.1820448877805, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=489, Percent_Identity=25.5623721881391, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI157426893, Length=174, Percent_Identity=29.3103448275862, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI94966754, Length=167, Percent_Identity=28.7425149700599, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=64.5593869731801, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=501, Percent_Identity=27.9441117764471, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI48994988, Length=194, Percent_Identity=28.8659793814433, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533571, Length=515, Percent_Identity=28.9320388349515, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=30.8988764044944, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=28.3018867924528, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=25.2427184466019, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=28.2608695652174, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=165, Percent_Identity=30.3030303030303, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=165, Percent_Identity=30.3030303030303, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=28.3333333333333, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI221458488, Length=505, Percent_Identity=24.5544554455446, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=28.735632183908, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24585711, Length=165, Percent_Identity=30.9090909090909, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585713, Length=165, Percent_Identity=30.9090909090909, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24585709, Length=165, Percent_Identity=30.9090909090909, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59902; Mature: 59770

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDPKKLNKEVAKRRTFAIISHPDAGKTTMTEKLLLWGKAIQVVGEVKGRKTDRHATSD
CCCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
WMSMEQERGISITTSVMQFPYKEHMVNLLDTPGHADFSEDTYRTLTAVDSALMMVDGAKG
HHHHHHHCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEECCCCC
VEERTIKLMEVCRMRDTPIISFVNKLDRQIREPLELLSEIEAVLKIKCIPVTWPIGMGQD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
FVGVYHLTENKTYFYEKGHGGDMTVAETREGYDYPDIRERLGALMFASFEESLELVQMAL
CEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EDFDVDEFLAGEMTPVLFGTALGNFGVNMVLDTLIKYAPPPKAHPTNEREVAATETTFSG
HHCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
FVFKIQANMDPRHRDRIAFLRVCSGKYEKGMKLKHVRLGKDVRIADALTFLAGDREALEE
EEEEEECCCCCCHHHHHHHHHHHCCCHHCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHH
AYPGDIIGLHNHGTISIGDSFTEGEELNFTGIPHFAPELFRRVILKDPLKSKALQKGLQQ
CCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LSEEGATQVFMPQINNDLILGAVGVLQFEVVAHRLKEEYKVQCIFEPVSIATVRWIHCDD
HHHCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEEEECCC
EVALAKFKRKAHDQLSLDGGGHLTYLAPSRVNLQLMQDRYPEVTFSNTREH
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCEEEEHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVDPKKLNKEVAKRRTFAIISHPDAGKTTMTEKLLLWGKAIQVVGEVKGRKTDRHATSD
CCCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
WMSMEQERGISITTSVMQFPYKEHMVNLLDTPGHADFSEDTYRTLTAVDSALMMVDGAKG
HHHHHHHCCCEEEHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEECCCCC
VEERTIKLMEVCRMRDTPIISFVNKLDRQIREPLELLSEIEAVLKIKCIPVTWPIGMGQD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCC
FVGVYHLTENKTYFYEKGHGGDMTVAETREGYDYPDIRERLGALMFASFEESLELVQMAL
CEEEEEEECCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EDFDVDEFLAGEMTPVLFGTALGNFGVNMVLDTLIKYAPPPKAHPTNEREVAATETTFSG
HHCCHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECC
FVFKIQANMDPRHRDRIAFLRVCSGKYEKGMKLKHVRLGKDVRIADALTFLAGDREALEE
EEEEEECCCCCCHHHHHHHHHHHCCCHHCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHH
AYPGDIIGLHNHGTISIGDSFTEGEELNFTGIPHFAPELFRRVILKDPLKSKALQKGLQQ
CCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LSEEGATQVFMPQINNDLILGAVGVLQFEVVAHRLKEEYKVQCIFEPVSIATVRWIHCDD
HHHCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEEEECCC
EVALAKFKRKAHDQLSLDGGGHLTYLAPSRVNLQLMQDRYPEVTFSNTREH
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCEEEEHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA