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The map label for this gene is yrkH [H]

Identifier: 93005251

GI number: 93005251

Start: 512331

End: 513683

Strand: Reverse

Name: yrkH [H]

Synonym: Pcryo_0421

Alternate gene names: 93005251

Gene position: 513683-512331 (Counterclockwise)

Preceding gene: 93005257

Following gene: 93005250

Centisome position: 16.79

GC content: 49.45

Gene sequence:

>1353_bases
ATGTTGCTTGAAAAGATTAAGACACCTGGGCTTGCCCATCTATCTTATCTGGTGGGGTCAGGTGGTCAAGCTGCAGTGAT
AGATCCTCGCCGTGACTGCGCGATTTATGTAGAAAAGGCACGGGCACAAGGGTTAGAAATCACACATATTTTCGAAACTC
ATCGCAACGAAGACCTTGTTTCCGGTGCTCCGATTCTAGCAGAAATGACCGGCGCCAGTATTCTTCACGGTCCGAACCCA
GAACAGCCTATCGTCTATGCTCAAACGGTGCGCGATGGTGATTGTTTTACCATCGGTCAGCTTGAAATACGCGTTTTAGA
AACGCCCGGTCATACTGATGACCATCTTGCCTATGCTCTATTTGACACAGCTTACCCCGATAAAGCTGTGGCGGTCTTCA
CCGGAGATGCCCTATTCGTCGGTGACGTCGGCCGTACTGACTTCTATCCTGATAGAGCTGAGGAAGTCGCAGGTCTACTC
TATGATTCGCTACAAAAAATCTTAGCACTAGGTGATCAGGCCATTATACATCCTGCACACGGCGCGGGCTCTGTCTGCGG
ATCAGGTATGGCAAAGCGTGAATTCTCTAGCGTCGGTCATGAAAGATTGAATAACCCACGTTTACAATTCACAGATCGCG
ACGCCTTTATCCGCTTTAAGGTCAGCGAAAACCATTATCAGCCGCCTTATTTCAGACTAATGGAGCGCTTGAACATGGAG
GGCGGTACTGCACCTCCGTCTGTTATTCGACCGCGCAGCTTTTCGCTACAGCAGTTGAAAAGCTGCGGTGCCGATCATCT
GATTGATATTCGTGAGCCGATGGCTTATGCTTCCGGTCATTTACCTGGAGCGATGAGCCTACCCGTGAGCATGATTTCCG
CCTTTGCCGGGTGGTACATCCATGAAGGCGAAAGCTTGGCATTAATTGCCTCTGATGAGGCACAACTCGCCACGGCTATG
GAGCATCTGGTTCGGATTGCCCTTGATAACGTATTAGGTGGCTATATCGGCGTGGTTTCCGCTGCTGCGAAAGGTGAAAA
AATGCAGCAAACCCCTATGATTGATACTAGCGAGGTTGAAGCTCGTCTAAAGAATGACACTGAGGGCTGGACCCTACTCG
ATGTCCGTGATGCCGACGAACGAGATACTGTCGCAATCGAGGGGTCACAACACATTTACGTTGGACATCTTAACAAATGT
TGGCGTGAACTTGATAAGAGACATCATTACACCTTGATGTGTGCTAGCGGAGCACGGGCCACAATAGCCGCAGGATGGCT
TGCAAGCCAAGGTTTTGAAAACATCGATATTTATCTTGGTTCGATGGGCGCATGGCAAGCAGCTCATAAATAA

Upstream 100 bases:

>100_bases
AAAACATCATCATACAACATTGATTATTCAAAGATTTTTATTAAAAGAGATAAAAACAATCATATGTTAGTCATTGTAAA
ATTATTAGAAGGAGACAGTT

Downstream 100 bases:

>100_bases
ATCTATAGATACAAGCTGTTTTTATGAGCAACGATTTGTTTTGACTAAATCAGTCTTATCGCCTTCACTTTATTGCAACG
ACTACTTATAGAGAGCTAGT

Product: beta-lactamase-like protein

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLLEKIKTPGLAHLSYLVGSGGQAAVIDPRRDCAIYVEKARAQGLEITHIFETHRNEDLVSGAPILAEMTGASILHGPNP
EQPIVYAQTVRDGDCFTIGQLEIRVLETPGHTDDHLAYALFDTAYPDKAVAVFTGDALFVGDVGRTDFYPDRAEEVAGLL
YDSLQKILALGDQAIIHPAHGAGSVCGSGMAKREFSSVGHERLNNPRLQFTDRDAFIRFKVSENHYQPPYFRLMERLNME
GGTAPPSVIRPRSFSLQQLKSCGADHLIDIREPMAYASGHLPGAMSLPVSMISAFAGWYIHEGESLALIASDEAQLATAM
EHLVRIALDNVLGGYIGVVSAAAKGEKMQQTPMIDTSEVEARLKNDTEGWTLLDVRDADERDTVAIEGSQHIYVGHLNKC
WRELDKRHHYTLMCASGARATIAAGWLASQGFENIDIYLGSMGAWQAAHK

Sequences:

>Translated_450_residues
MLLEKIKTPGLAHLSYLVGSGGQAAVIDPRRDCAIYVEKARAQGLEITHIFETHRNEDLVSGAPILAEMTGASILHGPNP
EQPIVYAQTVRDGDCFTIGQLEIRVLETPGHTDDHLAYALFDTAYPDKAVAVFTGDALFVGDVGRTDFYPDRAEEVAGLL
YDSLQKILALGDQAIIHPAHGAGSVCGSGMAKREFSSVGHERLNNPRLQFTDRDAFIRFKVSENHYQPPYFRLMERLNME
GGTAPPSVIRPRSFSLQQLKSCGADHLIDIREPMAYASGHLPGAMSLPVSMISAFAGWYIHEGESLALIASDEAQLATAM
EHLVRIALDNVLGGYIGVVSAAAKGEKMQQTPMIDTSEVEARLKNDTEGWTLLDVRDADERDTVAIEGSQHIYVGHLNKC
WRELDKRHHYTLMCASGARATIAAGWLASQGFENIDIYLGSMGAWQAAHK
>Mature_450_residues
MLLEKIKTPGLAHLSYLVGSGGQAAVIDPRRDCAIYVEKARAQGLEITHIFETHRNEDLVSGAPILAEMTGASILHGPNP
EQPIVYAQTVRDGDCFTIGQLEIRVLETPGHTDDHLAYALFDTAYPDKAVAVFTGDALFVGDVGRTDFYPDRAEEVAGLL
YDSLQKILALGDQAIIHPAHGAGSVCGSGMAKREFSSVGHERLNNPRLQFTDRDAFIRFKVSENHYQPPYFRLMERLNME
GGTAPPSVIRPRSFSLQQLKSCGADHLIDIREPMAYASGHLPGAMSLPVSMISAFAGWYIHEGESLALIASDEAQLATAM
EHLVRIALDNVLGGYIGVVSAAAKGEKMQQTPMIDTSEVEARLKNDTEGWTLLDVRDADERDTVAIEGSQHIYVGHLNKC
WRELDKRHHYTLMCASGARATIAAGWLASQGFENIDIYLGSMGAWQAAHK

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI21703352, Length=160, Percent_Identity=31.875, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI116642887, Length=171, Percent_Identity=32.7485380116959, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1787158, Length=206, Percent_Identity=30.0970873786408, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786406, Length=169, Percent_Identity=31.9526627218935, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538952, Length=209, Percent_Identity=29.1866028708134, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221330176, Length=229, Percent_Identity=30.5676855895196, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 49195; Mature: 49195

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEKIKTPGLAHLSYLVGSGGQAAVIDPRRDCAIYVEKARAQGLEITHIFETHRNEDLV
CCCHHHCCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEHHHCCCCEEEEEEHHCCCCCCC
SGAPILAEMTGASILHGPNPEQPIVYAQTVRDGDCFTIGQLEIRVLETPGHTDDHLAYAL
CCCCCEEHHCCCEEECCCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCCCCEEEEE
FDTAYPDKAVAVFTGDALFVGDVGRTDFYPDRAEEVAGLLYDSLQKILALGDQAIIHPAH
EECCCCCCEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GAGSVCGSGMAKREFSSVGHERLNNPRLQFTDRDAFIRFKVSENHYQPPYFRLMERLNME
CCCCHHCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCC
GGTAPPSVIRPRSFSLQQLKSCGADHLIDIREPMAYASGHLPGAMSLPVSMISAFAGWYI
CCCCCCCCCCCCCCCHHHHHHCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHCEEE
HEGESLALIASDEAQLATAMEHLVRIALDNVLGGYIGVVSAAAKGEKMQQTPMIDTSEVE
ECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHH
ARLKNDTEGWTLLDVRDADERDTVAIEGSQHIYVGHLNKCWRELDKRHHYTLMCASGARA
HHHCCCCCCEEEEEECCCCCCCEEEEECCCEEEEECHHHHHHHHHCCCCEEEEEECCCCC
TIAAGWLASQGFENIDIYLGSMGAWQAAHK
EEEEHHHHHCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MLLEKIKTPGLAHLSYLVGSGGQAAVIDPRRDCAIYVEKARAQGLEITHIFETHRNEDLV
CCCHHHCCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEHHHCCCCEEEEEEHHCCCCCCC
SGAPILAEMTGASILHGPNPEQPIVYAQTVRDGDCFTIGQLEIRVLETPGHTDDHLAYAL
CCCCCEEHHCCCEEECCCCCCCCEEEEEEECCCCEEEEEEEEEEEEECCCCCCCCEEEEE
FDTAYPDKAVAVFTGDALFVGDVGRTDFYPDRAEEVAGLLYDSLQKILALGDQAIIHPAH
EECCCCCCEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GAGSVCGSGMAKREFSSVGHERLNNPRLQFTDRDAFIRFKVSENHYQPPYFRLMERLNME
CCCCHHCCCHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHCCC
GGTAPPSVIRPRSFSLQQLKSCGADHLIDIREPMAYASGHLPGAMSLPVSMISAFAGWYI
CCCCCCCCCCCCCCCHHHHHHCCCCCEEECHHHHHHHCCCCCCCHHHHHHHHHHHHCEEE
HEGESLALIASDEAQLATAMEHLVRIALDNVLGGYIGVVSAAAKGEKMQQTPMIDTSEVE
ECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHH
ARLKNDTEGWTLLDVRDADERDTVAIEGSQHIYVGHLNKCWRELDKRHHYTLMCASGARA
HHHCCCCCCEEEEEECCCCCCCEEEEECCCEEEEECHHHHHHHHHCCCCEEEEEECCCCC
TIAAGWLASQGFENIDIYLGSMGAWQAAHK
EEEEHHHHHCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]