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The map label for this gene is tsf [H]

Identifier: 93005220

GI number: 93005220

Start: 475502

End: 476386

Strand: Direct

Name: tsf [H]

Synonym: Pcryo_0390

Alternate gene names: 93005220

Gene position: 475502-476386 (Clockwise)

Preceding gene: 93005219

Following gene: 93005221

Centisome position: 15.54

GC content: 43.62

Gene sequence:

>885_bases
ATGTCAGAAGTAAAAGTATCTGCCAAAATGGTAAAAGAATTGCGTGACCGTACTGGTCTTGGCATGATGGAATGTAAAAA
AGCGTTAGAAGAATCAAACGGTGATGTCGAAACTGCCATTGATAACCTACGTAAATCTGGTCAAGCAAAAGCGGCTAAAA
AAGCGGGTAACATCGCAGCTGACGGCGCTATCATCATCGCTCAAGGCGACAGCAAAGCATTCTTGCTAGAAGTGAACTGC
CAAACTGACTTCGTTGCAAAAGATGAAAACTTCGCAGCCTTTGCAGAAACAGTTGCAAACCTTGCATTAGAAAACAATGT
GACTGACGTAGCTGCTATCGCTGAATTGCCATATGGCAATGGTCAAACAGTTGAAGAAGCACGTGTATCTCTAGTACAAA
AAATCGGTGAGAATATCCAAATCCGCCGTGTTGAAGTACTTGAAGGCGCTAACATTGCTGCATACCGCCATGGTCTACGT
ATCGGCGTAGTGGTATCTTATGAAGGCGGCAGTGCAGAAACTGGTAAAAATCTTGCTATGCACATCGCTGCGTTCAACCC
AGTTGCTGTTGCTGATGAAGACGTCGCTGCTGATCTATTAGCGCGCGAAAAAGACATCATTGAAGCAAAAGCACGTGAGT
CTGGCAAGCCTGACAACATCGTTGAAAAAATGATCGAAGGTGGTCTACGTAAGTACCTAGAAGAAGTTACTTTATTACGT
CAGCCATACGTTATGGACAATGAGAAAAAAGTTGGTGACGTACTAAAAGCTGAAGGCGTAAAAGTACTTGGTTTCAAACG
TCTAGAAGTCGGTGAAGGCATTGAGAAAAAGCAAGAAGACTTCGCTGCTGAAGTTGCTGCTACTCAAGCACTAGCTAACA
AGTAA

Upstream 100 bases:

>100_bases
AAGTGGCGTTGTTAAGCCGCTATACAGAATTCAAGTGCGTTTAGATATTGTGCACTCCTATATATAATAATGCTCACCTA
CATAATAAAAGGTAACTCTT

Downstream 100 bases:

>100_bases
GTTTTAAAATGCAGTAAGTACTGAGTTTGTTTTTGCTCAGTACTTATAGATTGTTTGACATAAGAAGCAATCAAGCATAA
AAAAGCCCGTTATTTATTTG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSEVKVSAKMVKELRDRTGLGMMECKKALEESNGDVETAIDNLRKSGQAKAAKKAGNIAADGAIIIAQGDSKAFLLEVNC
QTDFVAKDENFAAFAETVANLALENNVTDVAAIAELPYGNGQTVEEARVSLVQKIGENIQIRRVEVLEGANIAAYRHGLR
IGVVVSYEGGSAETGKNLAMHIAAFNPVAVADEDVAADLLAREKDIIEAKARESGKPDNIVEKMIEGGLRKYLEEVTLLR
QPYVMDNEKKVGDVLKAEGVKVLGFKRLEVGEGIEKKQEDFAAEVAATQALANK

Sequences:

>Translated_294_residues
MSEVKVSAKMVKELRDRTGLGMMECKKALEESNGDVETAIDNLRKSGQAKAAKKAGNIAADGAIIIAQGDSKAFLLEVNC
QTDFVAKDENFAAFAETVANLALENNVTDVAAIAELPYGNGQTVEEARVSLVQKIGENIQIRRVEVLEGANIAAYRHGLR
IGVVVSYEGGSAETGKNLAMHIAAFNPVAVADEDVAADLLAREKDIIEAKARESGKPDNIVEKMIEGGLRKYLEEVTLLR
QPYVMDNEKKVGDVLKAEGVKVLGFKRLEVGEGIEKKQEDFAAEVAATQALANK
>Mature_293_residues
SEVKVSAKMVKELRDRTGLGMMECKKALEESNGDVETAIDNLRKSGQAKAAKKAGNIAADGAIIIAQGDSKAFLLEVNCQ
TDFVAKDENFAAFAETVANLALENNVTDVAAIAELPYGNGQTVEEARVSLVQKIGENIQIRRVEVLEGANIAAYRHGLRI
GVVVSYEGGSAETGKNLAMHIAAFNPVAVADEDVAADLLAREKDIIEAKARESGKPDNIVEKMIEGGLRKYLEEVTLLRQ
PYVMDNEKKVGDVLKAEGVKVLGFKRLEVGEGIEKKQEDFAAEVAATQALANK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=301, Percent_Identity=25.2491694352159, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI291084500, Length=323, Percent_Identity=24.4582043343653, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI291084498, Length=97, Percent_Identity=38.1443298969072, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI291084502, Length=103, Percent_Identity=36.8932038834951, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786366, Length=282, Percent_Identity=52.8368794326241, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17561440, Length=306, Percent_Identity=25.1633986928105, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921466, Length=303, Percent_Identity=26.7326732673267, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 31616; Mature: 31485

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVKVSAKMVKELRDRTGLGMMECKKALEESNGDVETAIDNLRKSGQAKAAKKAGNIAA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCEEC
DGAIIIAQGDSKAFLLEVNCQTDFVAKDENFAAFAETVANLALENNVTDVAAIAELPYGN
CCEEEEEECCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
GQTVEEARVSLVQKIGENIQIRRVEVLEGANIAAYRHGLRIGVVVSYEGGSAETGKNLAM
CCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCEEEEEEEECCCCCCCCCCEEE
HIAAFNPVAVADEDVAADLLAREKDIIEAKARESGKPDNIVEKMIEGGLRKYLEEVTLLR
EEEECCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QPYVMDNEKKVGDVLKAEGVKVLGFKRLEVGEGIEKKQEDFAAEVAATQALANK
CCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEVKVSAKMVKELRDRTGLGMMECKKALEESNGDVETAIDNLRKSGQAKAAKKAGNIAA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCEEC
DGAIIIAQGDSKAFLLEVNCQTDFVAKDENFAAFAETVANLALENNVTDVAAIAELPYGN
CCEEEEEECCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
GQTVEEARVSLVQKIGENIQIRRVEVLEGANIAAYRHGLRIGVVVSYEGGSAETGKNLAM
CCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCEEEEEEEECCCCCCCCCCEEE
HIAAFNPVAVADEDVAADLLAREKDIIEAKARESGKPDNIVEKMIEGGLRKYLEEVTLLR
EEEECCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QPYVMDNEKKVGDVLKAEGVKVLGFKRLEVGEGIEKKQEDFAAEVAATQALANK
CCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA