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The map label for this gene is rumA

Identifier: 93005208

GI number: 93005208

Start: 459410

End: 460951

Strand: Reverse

Name: rumA

Synonym: Pcryo_0378

Alternate gene names: 93005208

Gene position: 460951-459410 (Counterclockwise)

Preceding gene: 93005209

Following gene: 93005207

Centisome position: 15.06

GC content: 46.43

Gene sequence:

>1542_bases
ATGCAACCCACCGATTCAAAGACGCTTACCAACGTAGATGCAACCGAACAGGCAAATGAGACGCAAACCATTACGATTCC
GCCTAATAAGAAAAAATCTAAGCCATCATCAAAGACGCGTCGTCGTCTAAAAGATGCTGAGCCACTCCCCTTTACCATCG
ATGGCTTGTCACATGATGGGCGCGGCGTTGCGGTATACGGTAATGGCTTTGGTGAAACTGATGGTCATATCACAGACAAG
CATGGCAAAAAAATCTTTGTCAGCTTTGCCCTGCCAGGTGAAAGTGCGTTGGTCAAAATTACCAATAGCCGCACCAGCTT
TGAAGAGGGTGATGCCGTTAGAATCACTGCCAATCCAAACCCTGAGCGCGTGGTACCACCCTGCCCACATTTTGGTGTTT
GCGGTGGCTGTAATTTGCAGCATTGGCAGCCAGATGCACAAATTACATTTAAGCAATCTGTCCTTGCTGAGATGTTGGTC
CATCAAGCCAATGTGGCACCTGATCACTGGCTTGAGCCGGTAGTCGGTGATCGCCTTGGCTATCGTACCAAAGCCCGACT
GGGCGTACGTTATGTGGTCAAAAAAGAAACGGCTTTGGTCGGCTTTCGCGAGCGTTCAAGTAATTTCTTAGCAGAGTTAA
ATGAGTGTCATATCTTAGATCCGCGTATTGGCTTTGAGATTGAAAACTTAAAAACCCTTATTAGTACTTTAGATAGCCGT
GACAAGATTGCTCAGCTTGAGTTGGCAATGGGCGAGCATTTACCAGAGCTGCCTGATGGCGATCAGCCAGTCGCGCTAAT
TGTGCGTAATCTTGAACCGTTATCAGATGGCGATATTGATAAGCTAAAAGTATTTTTTGCAGCACGTAATTGGCAGCTGT
ATCTACAATCAAAGGGTGCCGATAGCATCCAACGTATTGCCTTGAGGGATACAGATGATTTAAGCGAGCAGTTTGGACGT
TTGTATTATCAATTACCAGAGTATGATTTGACGTTTGAATTTATTCCTACCGATTTTACCCAAGTTAATCTGTCGGTGAA
TCGTCAGATGACCAAGCTGGCTTGTGACTTGCTTGATTTAAAAGCAGGTGAACGCGTTCTAGATTTATTTAGCGGTTTAG
GCAATTTTAGCCTGCCTCTCGCGCGTTTGGTTGGTGAAACCGGCTCGGTCGTTGGTGTTGAAGGCAGTGAAGCTATGACT
GCACGCGCCGCTGATAACGCGCGCCGCAATGGCATTAATAACACTGAGTTTTATAGCCAAGATTTGACGCATGACTGTAC
GGACAAACCGTGGGCTAACCAAGGGTTTGATGCGCTGCTTATCGATCCACCGCGTTCTGGCGCGTGGGAGATTATGCAAT
ATTTGCCTAAGTTTAACGCCGAGCGGATTGTCTATGTCTCCTGTAATCCTGCTACACTTGCTCGCGATACCAAAGCATTG
CTTGAACAAGGTTATCGTTTGACTCACGCTGGCGTCATGGATATGTTTTGTCATACTGGTCACGTTGAATCCATCGCGCG
CTTTGAAAAAATATCTGCTTAA

Upstream 100 bases:

>100_bases
TAATCACCGCTTTTTTTACTTTGTGAGCGCCTAATACCTTAGTCGCTTTATATGTTAAAATTGCTATTATTTTTAACACC
TTCATTTTATAGGCAGGTTT

Downstream 100 bases:

>100_bases
ACCGTATGTAATGGCAGAGTATGGTATTAACCTATGATTTTTAGAGGCGTTAATAGCTGCAATTTAAGAATGATATCTGC
GTTACTCGCATATGCGGCTA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MQPTDSKTLTNVDATEQANETQTITIPPNKKKSKPSSKTRRRLKDAEPLPFTIDGLSHDGRGVAVYGNGFGETDGHITDK
HGKKIFVSFALPGESALVKITNSRTSFEEGDAVRITANPNPERVVPPCPHFGVCGGCNLQHWQPDAQITFKQSVLAEMLV
HQANVAPDHWLEPVVGDRLGYRTKARLGVRYVVKKETALVGFRERSSNFLAELNECHILDPRIGFEIENLKTLISTLDSR
DKIAQLELAMGEHLPELPDGDQPVALIVRNLEPLSDGDIDKLKVFFAARNWQLYLQSKGADSIQRIALRDTDDLSEQFGR
LYYQLPEYDLTFEFIPTDFTQVNLSVNRQMTKLACDLLDLKAGERVLDLFSGLGNFSLPLARLVGETGSVVGVEGSEAMT
ARAADNARRNGINNTEFYSQDLTHDCTDKPWANQGFDALLIDPPRSGAWEIMQYLPKFNAERIVYVSCNPATLARDTKAL
LEQGYRLTHAGVMDMFCHTGHVESIARFEKISA

Sequences:

>Translated_513_residues
MQPTDSKTLTNVDATEQANETQTITIPPNKKKSKPSSKTRRRLKDAEPLPFTIDGLSHDGRGVAVYGNGFGETDGHITDK
HGKKIFVSFALPGESALVKITNSRTSFEEGDAVRITANPNPERVVPPCPHFGVCGGCNLQHWQPDAQITFKQSVLAEMLV
HQANVAPDHWLEPVVGDRLGYRTKARLGVRYVVKKETALVGFRERSSNFLAELNECHILDPRIGFEIENLKTLISTLDSR
DKIAQLELAMGEHLPELPDGDQPVALIVRNLEPLSDGDIDKLKVFFAARNWQLYLQSKGADSIQRIALRDTDDLSEQFGR
LYYQLPEYDLTFEFIPTDFTQVNLSVNRQMTKLACDLLDLKAGERVLDLFSGLGNFSLPLARLVGETGSVVGVEGSEAMT
ARAADNARRNGINNTEFYSQDLTHDCTDKPWANQGFDALLIDPPRSGAWEIMQYLPKFNAERIVYVSCNPATLARDTKAL
LEQGYRLTHAGVMDMFCHTGHVESIARFEKISA
>Mature_513_residues
MQPTDSKTLTNVDATEQANETQTITIPPNKKKSKPSSKTRRRLKDAEPLPFTIDGLSHDGRGVAVYGNGFGETDGHITDK
HGKKIFVSFALPGESALVKITNSRTSFEEGDAVRITANPNPERVVPPCPHFGVCGGCNLQHWQPDAQITFKQSVLAEMLV
HQANVAPDHWLEPVVGDRLGYRTKARLGVRYVVKKETALVGFRERSSNFLAELNECHILDPRIGFEIENLKTLISTLDSR
DKIAQLELAMGEHLPELPDGDQPVALIVRNLEPLSDGDIDKLKVFFAARNWQLYLQSKGADSIQRIALRDTDDLSEQFGR
LYYQLPEYDLTFEFIPTDFTQVNLSVNRQMTKLACDLLDLKAGERVLDLFSGLGNFSLPLARLVGETGSVVGVEGSEAMT
ARAADNARRNGINNTEFYSQDLTHDCTDKPWANQGFDALLIDPPRSGAWEIMQYLPKFNAERIVYVSCNPATLARDTKAL
LEQGYRLTHAGVMDMFCHTGHVESIARFEKISA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=195, Percent_Identity=32.3076923076923, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI51173878, Length=195, Percent_Identity=32.3076923076923, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI1789148, Length=475, Percent_Identity=35.7894736842105, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1787083, Length=393, Percent_Identity=24.6819338422392, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790403, Length=191, Percent_Identity=30.3664921465969, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_PSYCK (Q1QDU2)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579645.1
- ProteinModelPortal:   Q1QDU2
- SMR:   Q1QDU2
- STRING:   Q1QDU2
- GeneID:   4034553
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0378
- NMPDR:   fig|335284.3.peg.1066
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   DEKEICH
- PhylomeDB:   Q1QDU2
- ProtClustDB:   PRK13168
- BioCyc:   PCRY335284:PCRYO_0378-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 57071; Mature: 57071

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 468-468 BINDING 341-341 BINDING 370-370 BINDING 375-375 BINDING 394-394 BINDING 421-421 BINDING 442-442

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPTDSKTLTNVDATEQANETQTITIPPNKKKSKPSSKTRRRLKDAEPLPFTIDGLSHDG
CCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCC
RGVAVYGNGFGETDGHITDKHGKKIFVSFALPGESALVKITNSRTSFEEGDAVRITANPN
CEEEEEECCCCCCCCEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCC
PERVVPPCPHFGVCGGCNLQHWQPDAQITFKQSVLAEMLVHQANVAPDHWLEPVVGDRLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHCCCHHHHHCC
YRTKARLGVRYVVKKETALVGFRERSSNFLAELNECHILDPRIGFEIENLKTLISTLDSR
CCHHHHCCEEEEEEECEEEEEEHHHCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCC
DKIAQLELAMGEHLPELPDGDQPVALIVRNLEPLSDGDIDKLKVFFAARNWQLYLQSKGA
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHEEEEEEECCEEEEEECCCC
DSIQRIALRDTDDLSEQFGRLYYQLPEYDLTFEFIPTDFTQVNLSVNRQMTKLACDLLDL
CHHHHHHCCCCHHHHHHHHHHEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHC
KAGERVLDLFSGLGNFSLPLARLVGETGSVVGVEGSEAMTARAADNARRNGINNTEFYSQ
CCCHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCCHHEEHHHHHHHHCCCCCCHHHHC
DLTHDCTDKPWANQGFDALLIDPPRSGAWEIMQYLPKFNAERIVYVSCNPATLARDTKAL
CCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
LEQGYRLTHAGVMDMFCHTGHVESIARFEKISA
HHCCCEEEHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQPTDSKTLTNVDATEQANETQTITIPPNKKKSKPSSKTRRRLKDAEPLPFTIDGLSHDG
CCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCC
RGVAVYGNGFGETDGHITDKHGKKIFVSFALPGESALVKITNSRTSFEEGDAVRITANPN
CEEEEEECCCCCCCCEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCC
PERVVPPCPHFGVCGGCNLQHWQPDAQITFKQSVLAEMLVHQANVAPDHWLEPVVGDRLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCHHHCCCHHHHHCC
YRTKARLGVRYVVKKETALVGFRERSSNFLAELNECHILDPRIGFEIENLKTLISTLDSR
CCHHHHCCEEEEEEECEEEEEEHHHCCHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCC
DKIAQLELAMGEHLPELPDGDQPVALIVRNLEPLSDGDIDKLKVFFAARNWQLYLQSKGA
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHEEEEEEECCEEEEEECCCC
DSIQRIALRDTDDLSEQFGRLYYQLPEYDLTFEFIPTDFTQVNLSVNRQMTKLACDLLDL
CHHHHHHCCCCHHHHHHHHHHEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHC
KAGERVLDLFSGLGNFSLPLARLVGETGSVVGVEGSEAMTARAADNARRNGINNTEFYSQ
CCCHHHHHHHHCCCCCCCHHHHHHCCCCCEEEECCCCHHEEHHHHHHHHCCCCCCHHHHC
DLTHDCTDKPWANQGFDALLIDPPRSGAWEIMQYLPKFNAERIVYVSCNPATLARDTKAL
CCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHH
LEQGYRLTHAGVMDMFCHTGHVESIARFEKISA
HHCCCEEEHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA