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The map label for this gene is mscL

Identifier: 93005204

GI number: 93005204

Start: 454248

End: 454697

Strand: Direct

Name: mscL

Synonym: Pcryo_0374

Alternate gene names: 93005204

Gene position: 454248-454697 (Clockwise)

Preceding gene: 93005203

Following gene: 93005211

Centisome position: 14.85

GC content: 40.44

Gene sequence:

>450_bases
ATGAGTATGGTATCTGAGTTTAAAGAATTTGCTTTAAAAGGTAATGTGATGGATCTGGCCGTCGGTGTGATCATCGGTGG
AGCATTCGCTACGATCACAAAGTCTTTAGTAGAAGACGTGATCATGCCCATTGTTGCTTTTATCGTCGGCGGCGAAATTA
ATTTCAAGAATATGTTTTTGATATTGGGCGATGCGCCAGAAGGTGTGGCACGAACCAATGATGCCCTCAAAGCTGCTGGC
ATTCCTGTATTGGCTTATGGTAGTTTCATTACGGTGCTAATTAACTTTCTAATCTTGGCGTTTATTATTTTTATGATGGT
AAAAATGGTCAATAGATTGCGCCGTGCAGATGAGGTAGAAGAAGCAATAGAAGAAGCAATAGAAGAGCCGTCAGAAGAAG
TGCAATTGCTTCGTGAAATTAGCGCTAAGCTTGGCAATATTAATAAGTAA

Upstream 100 bases:

>100_bases
AAAAAAACGAGTAATCTTACGGTTATTATTAAAATCCCAAGTTGGATGCATCATTTATACGCTTATCTTATGGTCAATAA
GATTTTTGAGGAGATAAATC

Downstream 100 bases:

>100_bases
ACACATTTTACTGACCGTCAGATTAAAAAATAAAAAAAGCTAACGTAATGTTAGCCTCTTTTTATTGGATTTCTTATAAT
TACTTAATGGTAGTTATGTT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSMVSEFKEFALKGNVMDLAVGVIIGGAFATITKSLVEDVIMPIVAFIVGGEINFKNMFLILGDAPEGVARTNDALKAAG
IPVLAYGSFITVLINFLILAFIIFMMVKMVNRLRRADEVEEAIEEAIEEPSEEVQLLREISAKLGNINK

Sequences:

>Translated_149_residues
MSMVSEFKEFALKGNVMDLAVGVIIGGAFATITKSLVEDVIMPIVAFIVGGEINFKNMFLILGDAPEGVARTNDALKAAG
IPVLAYGSFITVLINFLILAFIIFMMVKMVNRLRRADEVEEAIEEAIEEPSEEVQLLREISAKLGNINK
>Mature_148_residues
SMVSEFKEFALKGNVMDLAVGVIIGGAFATITKSLVEDVIMPIVAFIVGGEINFKNMFLILGDAPEGVARTNDALKAAGI
PVLAYGSFITVLINFLILAFIIFMMVKMVNRLRRADEVEEAIEEAIEEPSEEVQLLREISAKLGNINK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=150, Percent_Identity=45.3333333333333, Blast_Score=104, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PSYCK (Q1QDU6)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579641.1
- ProteinModelPortal:   Q1QDU6
- SMR:   Q1QDU6
- STRING:   Q1QDU6
- GeneID:   4034549
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0374
- NMPDR:   fig|335284.3.peg.1062
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   FATITKS
- PhylomeDB:   Q1QDU6
- ProtClustDB:   PRK13952
- BioCyc:   PCRY335284:PCRYO_0374-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16251; Mature: 16119

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe9d4c1c)-; HASH(0x10dc3d38)-; HASH(0x11185004)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMVSEFKEFALKGNVMDLAVGVIIGGAFATITKSLVEDVIMPIVAFIVGGEINFKNMFL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
ILGDAPEGVARTNDALKAAGIPVLAYGSFITVLINFLILAFIIFMMVKMVNRLRRADEVE
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAIEEAIEEPSEEVQLLREISAKLGNINK
HHHHHHHHCCHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
SMVSEFKEFALKGNVMDLAVGVIIGGAFATITKSLVEDVIMPIVAFIVGGEINFKNMFL
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
ILGDAPEGVARTNDALKAAGIPVLAYGSFITVLINFLILAFIIFMMVKMVNRLRRADEVE
EECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAIEEAIEEPSEEVQLLREISAKLGNINK
HHHHHHHHCCHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA