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The map label for this gene is purK [H]

Identifier: 93005133

GI number: 93005133

Start: 362504

End: 363859

Strand: Reverse

Name: purK [H]

Synonym: Pcryo_0302

Alternate gene names: 93005133

Gene position: 363859-362504 (Counterclockwise)

Preceding gene: 93005134

Following gene: 93005132

Centisome position: 11.89

GC content: 44.91

Gene sequence:

>1356_bases
ATGACCATAGCGAACGCTTACCCTGTTACTCAAACTATCCGCACCATTGGTATTTTAGGCGGTGGTCAGCTTGGCATGAT
GCTGGCACAAGCGGCTATGCCATTGGGCTATCACTGTGTGTTTTTGGAAGACAGCGCTGACTGTCCGGCCAGTCTATATG
GGCGCGTTTTTAGCAGCGAGCAATTAGATGCTTTTATTGAAGCAGCTGACGTCTTCACTTTGGAGTTTGAAAATACCCCA
ACTGCTACCGTTCAGCAACTAGCAAATCTATCAAAATCTGGCAGCAAACAAGGCATGTTTCCGCCAACGATTGCCCTAGA
TATTGCGCAAGATCGCTTAAAAGAAAAGCAGATGTTTAATACGCTTGATATCGCAACCGTACCTTTCGTGTCTGTTAGTT
CAGAGGCGGAATTACAGCAAGCTTGTCATAAGCTTGGATTACCTATCGTACTTAAAACCAGTCGCGGCGGTTATGATGGC
AAAGGTCAGTTTGTGATCAGACAAGACAGTGATATCAAAGCCGCTTGGCAAGAGCTAAAGGATGCAGTTACGGGTACAGG
CTCACTGACGCAAACGCCAGCGCCATTGATTGCTGAAGGTTTTATTCACTTTAGTCGGGAGCTTTCTATCATCGCCGCAC
GAGGTCAAAACGGTCAGGTACGCTGTTATGATTTGGTCGAAAACCATCATCATCAGGGTATCTTGGCCAAAACCCAAGCA
CCAGCTATTGGTACCAGTCATTTATTCAAACAAGCAACTGATGCTATTACCAACGTCATGAATCATTTGGGCTATGTTGG
GGTTATGGCGCTTGAGCTATTTGTGACCAAAGACGCTCGCGGCAATGATACTCTACTTGCCAACGAGATAGCGCCACGCG
TGCACAATTCTGGTCATTGGAGTATTGAAGGTGCTGTCACCAGCCAATTTGAGAATCATATTCGTGCCGTAGTCAATCTG
CCACTAGGCGATACTGACAATGTCTATCCAGCAATTATGATTAATGTCTTGGGGCAATATCCTGATATCAGCGAAGTGCT
CAATATCGACGGCGTGCATTATCACAGCTATCATAAAGCGGAGCGCGATGACCGTAAAATTGCCCATATTACCTTGATGC
CCAATGATGTAGCTGACTTAGAGCCTGCATTGGCAAAGCTGGTCGCCGTACTGCCTAATAAAGTTGGTCTTGATAAAAAG
GTAACGTCTATCAATAACCCGATAACAGCGGAAGATGATCTAATTACTGCTAAAGTAAAGGCTGACGCTGAAAATAGAGA
AAGCTTAGCAAATGATGATAATGAGCAACAAAATTTTGAGTCAACCAACTCTAAAGCAGAGAAGGCTAAGAAATAA

Upstream 100 bases:

>100_bases
TTAACTCGTTAGACTTTTATAGCCTTTATAGCCATATGCATTTATATTTTTATATATTGAAGCATTTAACCCCTTGTTTA
TTTGAATCAAAGAGCCTGCC

Downstream 100 bases:

>100_bases
TGCAGATTTGGGTAGATGCAGATTCAGTGCCTTTGATTGCCAAAGACTTGATTATTAAGACCGCTGAGCGTACACAAACG
ATGGCGATATTTGTCGCCAA

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTIANAYPVTQTIRTIGILGGGQLGMMLAQAAMPLGYHCVFLEDSADCPASLYGRVFSSEQLDAFIEAADVFTLEFENTP
TATVQQLANLSKSGSKQGMFPPTIALDIAQDRLKEKQMFNTLDIATVPFVSVSSEAELQQACHKLGLPIVLKTSRGGYDG
KGQFVIRQDSDIKAAWQELKDAVTGTGSLTQTPAPLIAEGFIHFSRELSIIAARGQNGQVRCYDLVENHHHQGILAKTQA
PAIGTSHLFKQATDAITNVMNHLGYVGVMALELFVTKDARGNDTLLANEIAPRVHNSGHWSIEGAVTSQFENHIRAVVNL
PLGDTDNVYPAIMINVLGQYPDISEVLNIDGVHYHSYHKAERDDRKIAHITLMPNDVADLEPALAKLVAVLPNKVGLDKK
VTSINNPITAEDDLITAKVKADAENRESLANDDNEQQNFESTNSKAEKAKK

Sequences:

>Translated_451_residues
MTIANAYPVTQTIRTIGILGGGQLGMMLAQAAMPLGYHCVFLEDSADCPASLYGRVFSSEQLDAFIEAADVFTLEFENTP
TATVQQLANLSKSGSKQGMFPPTIALDIAQDRLKEKQMFNTLDIATVPFVSVSSEAELQQACHKLGLPIVLKTSRGGYDG
KGQFVIRQDSDIKAAWQELKDAVTGTGSLTQTPAPLIAEGFIHFSRELSIIAARGQNGQVRCYDLVENHHHQGILAKTQA
PAIGTSHLFKQATDAITNVMNHLGYVGVMALELFVTKDARGNDTLLANEIAPRVHNSGHWSIEGAVTSQFENHIRAVVNL
PLGDTDNVYPAIMINVLGQYPDISEVLNIDGVHYHSYHKAERDDRKIAHITLMPNDVADLEPALAKLVAVLPNKVGLDKK
VTSINNPITAEDDLITAKVKADAENRESLANDDNEQQNFESTNSKAEKAKK
>Mature_450_residues
TIANAYPVTQTIRTIGILGGGQLGMMLAQAAMPLGYHCVFLEDSADCPASLYGRVFSSEQLDAFIEAADVFTLEFENTPT
ATVQQLANLSKSGSKQGMFPPTIALDIAQDRLKEKQMFNTLDIATVPFVSVSSEAELQQACHKLGLPIVLKTSRGGYDGK
GQFVIRQDSDIKAAWQELKDAVTGTGSLTQTPAPLIAEGFIHFSRELSIIAARGQNGQVRCYDLVENHHHQGILAKTQAP
AIGTSHLFKQATDAITNVMNHLGYVGVMALELFVTKDARGNDTLLANEIAPRVHNSGHWSIEGAVTSQFENHIRAVVNLP
LGDTDNVYPAIMINVLGQYPDISEVLNIDGVHYHSYHKAERDDRKIAHITLMPNDVADLEPALAKLVAVLPNKVGLDKKV
TSINNPITAEDDLITAKVKADAENRESLANDDNEQQNFESTNSKAEKAKK

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=382, Percent_Identity=35.3403141361257, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788155, Length=330, Percent_Identity=23.9393939393939, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=412, Percent_Identity=31.5533980582524, Blast_Score=180, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 49024; Mature: 48893

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIANAYPVTQTIRTIGILGGGQLGMMLAQAAMPLGYHCVFLEDSADCPASLYGRVFSSE
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCH
QLDAFIEAADVFTLEFENTPTATVQQLANLSKSGSKQGMFPPTIALDIAQDRLKEKQMFN
HHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
TLDIATVPFVSVSSEAELQQACHKLGLPIVLKTSRGGYDGKGQFVIRQDSDIKAAWQELK
HHHHHHCCEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHH
DAVTGTGSLTQTPAPLIAEGFIHFSRELSIIAARGQNGQVRCYDLVENHHHQGILAKTQA
HHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEHHHCCCCCCEEEECCC
PAIGTSHLFKQATDAITNVMNHLGYVGVMALELFVTKDARGNDTLLANEIAPRVHNSGHW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEHHHHHHCCCCCCCE
SIEGAVTSQFENHIRAVVNLPLGDTDNVYPAIMINVLGQYPDISEVLNIDGVHYHSYHKA
EEEHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEEECCHHHC
ERDDRKIAHITLMPNDVADLEPALAKLVAVLPNKVGLDKKVTSINNPITAEDDLITAKVK
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEEEE
ADAENRESLANDDNEQQNFESTNSKAEKAKK
CCCHHHHHHCCCCCHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
TIANAYPVTQTIRTIGILGGGQLGMMLAQAAMPLGYHCVFLEDSADCPASLYGRVFSSE
CCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCH
QLDAFIEAADVFTLEFENTPTATVQQLANLSKSGSKQGMFPPTIALDIAQDRLKEKQMFN
HHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
TLDIATVPFVSVSSEAELQQACHKLGLPIVLKTSRGGYDGKGQFVIRQDSDIKAAWQELK
HHHHHHCCEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHH
DAVTGTGSLTQTPAPLIAEGFIHFSRELSIIAARGQNGQVRCYDLVENHHHQGILAKTQA
HHHCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEEHHHCCCCCCEEEECCC
PAIGTSHLFKQATDAITNVMNHLGYVGVMALELFVTKDARGNDTLLANEIAPRVHNSGHW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEHHHHHHCCCCCCCE
SIEGAVTSQFENHIRAVVNLPLGDTDNVYPAIMINVLGQYPDISEVLNIDGVHYHSYHKA
EEEHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCEEECCHHHC
ERDDRKIAHITLMPNDVADLEPALAKLVAVLPNKVGLDKKVTSINNPITAEDDLITAKVK
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCEEEEEEE
ADAENRESLANDDNEQQNFESTNSKAEKAKK
CCCHHHHHHCCCCCHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8816818; 10984043 [H]