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The map label for this gene is pdxB

Identifier: 93005129

GI number: 93005129

Start: 358857

End: 360017

Strand: Direct

Name: pdxB

Synonym: Pcryo_0298

Alternate gene names: 93005129

Gene position: 358857-360017 (Clockwise)

Preceding gene: 93005128

Following gene: 93005130

Centisome position: 11.73

GC content: 41.52

Gene sequence:

>1161_bases
ATGAAAAATCTCACTATTGTGGCTGATAGTAATATCGCAAGCCTTGATGAGTTCTTTAATCCTGTAGCGCTGGGTCAAAA
TACTGAGCAACAGGTGCAGGTCATACGTGTTGCTGGTCGTGATATCAATGCACAATTAATGGCAGATGTGCAGCCTGACG
TTTTGTTGATACGCTCTGTCACTTCAATAAATGAATCACTATTAAGTGATAATAATAGCGTCAAATTCGTTGGTTCTGCG
ACCATTGGTACTGACCATGTCGATCAAGAATATTTGGCAGAACGCAATATTACCTTTGCCAATGCCGCTGGCTGTAGCAA
GCATTCAGTCGCACAATACGTAGTGACTGCTATATTAACTTTACGCCCACAGTATTGGCAGCAATCCACGAAGCCATTAA
CAGTGGGCATCATCGGACTTGGTAATATCGGTAGTACGCTAGCTCAGTATGCCAATGATTTAGGCTGGAAAATATTGGGT
TATGACCCTTTATTAGCCACATCCGATATCAATAACGCTAGTCTTGAGCAGGTATTGAGCCAAAGCGACATAGTAAGTTT
ACATGTTCCTTTAACGGATAAAAAAGATACGGATGCGCAAGATGCAGTTAGCAACAGCATTAGCAATAATGTCAGCGATT
ATCCAACGCGTCATTTAATTAATGCAGAAACTTTGACACTGATGTCGCCGCATACTATGCTGATTAATAGTGCGCGCGGA
CCTGTGATTGATGCCGCTGCCCTCGAAGCCGATATCGATGCGACTGAACGCCAAGTGGTATTGGATGTGTTTGAACATGA
GCCGCAGATTTCTGAGAGTTTGCTATCAAAGCTTGCTATTGCCACTCCGCATATTGCTGGTTATACCTTAGAAGGTAAGC
TGCGTGGCACGCAAATTATTTATGATGCCTTATGTGAAAAATTGGCAGTACTACCTGTATTATCGATGCATCAGTTACTA
CCACTCAATACTTATCTATGGTTTGAGCTAAAAGAACATCCAGACAGATTGCAAAAGTTTTATGACATTAAAAAAGACGA
TGCGGCATTAAGAAATAAAATAACCAACGGTAAAGTTAAAGGTTCAGATTTTGATCAGTTACGCCGTGATTACCATCTGC
GCCGTGAATGGCAAGCACAAACAATCAGTGTGACTATTTAA

Upstream 100 bases:

>100_bases
TTTTATAGTAAAGAATCATCTAAAGCGCTTCAAGTCATATATAAGATTATCTATATTCAATGAGACGGTAGCGAATACGA
TGAAAAACAAGGTAGATACG

Downstream 100 bases:

>100_bases
TTATCTGTTTTTACGATTTATGAGGCACTGTCACCAGTCATGAGTAAAGCCAATAGTCAGCCTTTATCTAATCTAAGCTA
TGCCCCAACGATGACCTTTG

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKNLTIVADSNIASLDEFFNPVALGQNTEQQVQVIRVAGRDINAQLMADVQPDVLLIRSVTSINESLLSDNNSVKFVGSA
TIGTDHVDQEYLAERNITFANAAGCSKHSVAQYVVTAILTLRPQYWQQSTKPLTVGIIGLGNIGSTLAQYANDLGWKILG
YDPLLATSDINNASLEQVLSQSDIVSLHVPLTDKKDTDAQDAVSNSISNNVSDYPTRHLINAETLTLMSPHTMLINSARG
PVIDAAALEADIDATERQVVLDVFEHEPQISESLLSKLAIATPHIAGYTLEGKLRGTQIIYDALCEKLAVLPVLSMHQLL
PLNTYLWFELKEHPDRLQKFYDIKKDDAALRNKITNGKVKGSDFDQLRRDYHLRREWQAQTISVTI

Sequences:

>Translated_386_residues
MKNLTIVADSNIASLDEFFNPVALGQNTEQQVQVIRVAGRDINAQLMADVQPDVLLIRSVTSINESLLSDNNSVKFVGSA
TIGTDHVDQEYLAERNITFANAAGCSKHSVAQYVVTAILTLRPQYWQQSTKPLTVGIIGLGNIGSTLAQYANDLGWKILG
YDPLLATSDINNASLEQVLSQSDIVSLHVPLTDKKDTDAQDAVSNSISNNVSDYPTRHLINAETLTLMSPHTMLINSARG
PVIDAAALEADIDATERQVVLDVFEHEPQISESLLSKLAIATPHIAGYTLEGKLRGTQIIYDALCEKLAVLPVLSMHQLL
PLNTYLWFELKEHPDRLQKFYDIKKDDAALRNKITNGKVKGSDFDQLRRDYHLRREWQAQTISVTI
>Mature_386_residues
MKNLTIVADSNIASLDEFFNPVALGQNTEQQVQVIRVAGRDINAQLMADVQPDVLLIRSVTSINESLLSDNNSVKFVGSA
TIGTDHVDQEYLAERNITFANAAGCSKHSVAQYVVTAILTLRPQYWQQSTKPLTVGIIGLGNIGSTLAQYANDLGWKILG
YDPLLATSDINNASLEQVLSQSDIVSLHVPLTDKKDTDAQDAVSNSISNNVSDYPTRHLINAETLTLMSPHTMLINSARG
PVIDAAALEADIDATERQVVLDVFEHEPQISESLLSKLAIATPHIAGYTLEGKLRGTQIIYDALCEKLAVLPVLSMHQLL
PLNTYLWFELKEHPDRLQKFYDIKKDDAALRNKITNGKVKGSDFDQLRRDYHLRREWQAQTISVTI

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=276, Percent_Identity=24.6376811594203, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4557497, Length=166, Percent_Identity=33.1325301204819, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI61743967, Length=167, Percent_Identity=32.9341317365269, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788660, Length=389, Percent_Identity=35.4755784061697, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789279, Length=267, Percent_Identity=28.8389513108614, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082289, Length=300, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787645, Length=218, Percent_Identity=23.8532110091743, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=297, Percent_Identity=28.6195286195286, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=272, Percent_Identity=29.0441176470588, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=317, Percent_Identity=27.1293375394322, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320925, Length=272, Percent_Identity=28.6764705882353, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19921140, Length=274, Percent_Identity=27.3722627737226, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24646446, Length=242, Percent_Identity=29.3388429752066, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646448, Length=242, Percent_Identity=29.3388429752066, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646452, Length=242, Percent_Identity=29.3388429752066, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646450, Length=242, Percent_Identity=29.3388429752066, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62472511, Length=242, Percent_Identity=29.3388429752066, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_PSYCK (Q1QE21)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579566.1
- STRING:   Q1QE21
- GeneID:   4034508
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0298
- NMPDR:   fig|335284.3.peg.987
- eggNOG:   COG0111
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- PhylomeDB:   Q1QE21
- ProtClustDB:   CLSK838887
- BioCyc:   PCRY335284:PCRYO_0298-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 42644; Mature: 42644

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 239-239 ACT_SITE 267-267 ACT_SITE 284-284 BINDING 59-59 BINDING 81-81 BINDING 162-162 BINDING 262-262 BINDING 287-287 BINDING 288-288

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLTIVADSNIASLDEFFNPVALGQNTEQQVQVIRVAGRDINAQLMADVQPDVLLIRSV
CCCEEEEECCCHHHHHHHCCCHHCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEHHH
TSINESLLSDNNSVKFVGSATIGTDHVDQEYLAERNITFANAAGCSKHSVAQYVVTAILT
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHH
LRPQYWQQSTKPLTVGIIGLGNIGSTLAQYANDLGWKILGYDPLLATSDINNASLEQVLS
CCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCEEEECCCCHHHHHHHHC
QSDIVSLHVPLTDKKDTDAQDAVSNSISNNVSDYPTRHLINAETLTLMSPHTMLINSARG
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEECCCCEEEECCCC
PVIDAAALEADIDATERQVVLDVFEHEPQISESLLSKLAIATPHIAGYTLEGKLRGTQII
CEEEHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
YDALCEKLAVLPVLSMHQLLPLNTYLWFELKEHPDRLQKFYDIKKDDAALRNKITNGKVK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHCCCCCCCC
GSDFDQLRRDYHLRREWQAQTISVTI
CCCHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MKNLTIVADSNIASLDEFFNPVALGQNTEQQVQVIRVAGRDINAQLMADVQPDVLLIRSV
CCCEEEEECCCHHHHHHHCCCHHCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEEHHH
TSINESLLSDNNSVKFVGSATIGTDHVDQEYLAERNITFANAAGCSKHSVAQYVVTAILT
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHH
LRPQYWQQSTKPLTVGIIGLGNIGSTLAQYANDLGWKILGYDPLLATSDINNASLEQVLS
CCCHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEECCCCEEEECCCCHHHHHHHHC
QSDIVSLHVPLTDKKDTDAQDAVSNSISNNVSDYPTRHLINAETLTLMSPHTMLINSARG
CCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEECCCCEEEECCCC
PVIDAAALEADIDATERQVVLDVFEHEPQISESLLSKLAIATPHIAGYTLEGKLRGTQII
CEEEHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHH
YDALCEKLAVLPVLSMHQLLPLNTYLWFELKEHPDRLQKFYDIKKDDAALRNKITNGKVK
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHCCCCCCCC
GSDFDQLRRDYHLRREWQAQTISVTI
CCCHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA