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The map label for this gene is wax-dgaT [H]

Identifier: 93005078

GI number: 93005078

Start: 290771

End: 292210

Strand: Reverse

Name: wax-dgaT [H]

Synonym: Pcryo_0247

Alternate gene names: 93005078

Gene position: 292210-290771 (Counterclockwise)

Preceding gene: 93005079

Following gene: 93005075

Centisome position: 9.55

GC content: 43.61

Gene sequence:

>1440_bases
ATGCGACTTCTTACAGCAGTCGACCAGTTGTTTTTACTTCTTGAGTCGCGTAAACAACCCATGCATGTTGGAGGTCTATT
TTTATTTGAATTACCAGAGGATGCCGATATCAGTTTTGTTCATCAGCTTGTAAAGCAGATGCAAGACTCTCATGTACCGC
CAACGTTTCCGTTCAACCAAGTGCTTGAGCATATGGTATTTTGGAAAAAAGACAAAAATTTTGATGTCGAGCACCACTTG
CATCATGTCGCCCTACCCAAGCCCGCCCGTGTGCGCGAACTTTTGATGTATGTTTCAAGAGAGCATGGTCGTCTATTAGA
CCGTGCTATGCCCCTGTGGGAATGCCATGTGATTGAAGGCATTCAGCCAGAGAGCGAAGGTAGCCCAGAGCGCTTTGCGC
TGTATTTCAAAATCCATCACTCGCTGGTTGATGGCATCGCTGCCATGCGTTTGGTCAAAAAATCCTTGTCACAATCGCCC
AATGAGCCAGTTACTTTGCCTATTTGGTCATTAATGGCACGCCATCGCAATCAGATCGATGCTATCTTGCCCAAAGAGCG
CTCCGCTTTACGCATCCTGAAAGAGCAAGTATCTACCATTAAGCCTGTGTTCACAGAATTACTGGATAATTTTAAAAATT
ATAATGACGACAGCTATGTCAGTACCTTTGATGCCCCGAGAAGTATTCTAAACAGACGCATCTCAGCATCGAGGCGCATA
GCGGCACAATCTTATGATATTAAGCGCTTCAACGATATTGCTGAGCGTATTAATATTAGCAAAAACGATGTAGTACTAGC
CGTTTGTGCTGGTGCTATCCGGCGTTATTTGATTTCGATGGATGCACTACCTAGCAAGCCTCTGATTGCGTTCGTGCCCA
TGTCTTTACGAACTGACGATAGTGTCGCGGGCAATCAGCTGTCATTTGTCCTTGCCAATCTGGGTACTCATCTGGATGAT
CCATTGAGTAGAATCAAGCTAATTCATCGCAGTATGAACAATGGAAAACGCCGTTTTAGACGTATGAATCAAGCGCAAGT
TATTAACTACAGTGTGGTGTCTTATGCATGGGAAGGGATTAATCTGGCGACTGGTTTGTTTCCTAAAAAACAAGCCTTCA
ACCTGATTATCTCAAATGTACCAGGCTCCGAAAAATCATTGTATTGGAATGGCGCGCGCCTACAATCTTTGTACCCTGCC
TCGATTGTCTTCAACGGGCAAGCGATGAATATTACGCTGGCAAGTTATTTGGATAAGATTGAATTTGGTATTACTGCCTG
TAGCAAGGCCTTACCTCATGTGCAAGATATGCTGATGCTGATAGAAGAAGAGTTGCAGCTGCTTGAAAAGGTTAGTAAAG
AGCTTGAATTCAATGGTATTACAGTTGAAGATAAAAGTGGCTATAAAGGTAATGGTAAAACAAAAAAGCTAGCTCCGTAA

Upstream 100 bases:

>100_bases
ATGCGCAAAATGGCAACTGCCAGCTCACCACAGACGGGATACTTTTTTCAGTAGTATTAAAGGTTAGGTGATTATATTTT
TCATTATAAAAGGATTTATT

Downstream 100 bases:

>100_bases
CGGAACCAGCTCTTTATTTGTCATATTTAGCTATCTAAAAGGGGACGATTAAAGCGCATCGCCCCATGCTGGCACAACCA
CTTGCATCAAAGGTTTTAAC

Product: hypothetical protein

Products: NA

Alternate protein names: Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRLLTAVDQLFLLLESRKQPMHVGGLFLFELPEDADISFVHQLVKQMQDSHVPPTFPFNQVLEHMVFWKKDKNFDVEHHL
HHVALPKPARVRELLMYVSREHGRLLDRAMPLWECHVIEGIQPESEGSPERFALYFKIHHSLVDGIAAMRLVKKSLSQSP
NEPVTLPIWSLMARHRNQIDAILPKERSALRILKEQVSTIKPVFTELLDNFKNYNDDSYVSTFDAPRSILNRRISASRRI
AAQSYDIKRFNDIAERINISKNDVVLAVCAGAIRRYLISMDALPSKPLIAFVPMSLRTDDSVAGNQLSFVLANLGTHLDD
PLSRIKLIHRSMNNGKRRFRRMNQAQVINYSVVSYAWEGINLATGLFPKKQAFNLIISNVPGSEKSLYWNGARLQSLYPA
SIVFNGQAMNITLASYLDKIEFGITACSKALPHVQDMLMLIEEELQLLEKVSKELEFNGITVEDKSGYKGNGKTKKLAP

Sequences:

>Translated_479_residues
MRLLTAVDQLFLLLESRKQPMHVGGLFLFELPEDADISFVHQLVKQMQDSHVPPTFPFNQVLEHMVFWKKDKNFDVEHHL
HHVALPKPARVRELLMYVSREHGRLLDRAMPLWECHVIEGIQPESEGSPERFALYFKIHHSLVDGIAAMRLVKKSLSQSP
NEPVTLPIWSLMARHRNQIDAILPKERSALRILKEQVSTIKPVFTELLDNFKNYNDDSYVSTFDAPRSILNRRISASRRI
AAQSYDIKRFNDIAERINISKNDVVLAVCAGAIRRYLISMDALPSKPLIAFVPMSLRTDDSVAGNQLSFVLANLGTHLDD
PLSRIKLIHRSMNNGKRRFRRMNQAQVINYSVVSYAWEGINLATGLFPKKQAFNLIISNVPGSEKSLYWNGARLQSLYPA
SIVFNGQAMNITLASYLDKIEFGITACSKALPHVQDMLMLIEEELQLLEKVSKELEFNGITVEDKSGYKGNGKTKKLAP
>Mature_479_residues
MRLLTAVDQLFLLLESRKQPMHVGGLFLFELPEDADISFVHQLVKQMQDSHVPPTFPFNQVLEHMVFWKKDKNFDVEHHL
HHVALPKPARVRELLMYVSREHGRLLDRAMPLWECHVIEGIQPESEGSPERFALYFKIHHSLVDGIAAMRLVKKSLSQSP
NEPVTLPIWSLMARHRNQIDAILPKERSALRILKEQVSTIKPVFTELLDNFKNYNDDSYVSTFDAPRSILNRRISASRRI
AAQSYDIKRFNDIAERINISKNDVVLAVCAGAIRRYLISMDALPSKPLIAFVPMSLRTDDSVAGNQLSFVLANLGTHLDD
PLSRIKLIHRSMNNGKRRFRRMNQAQVINYSVVSYAWEGINLATGLFPKKQAFNLIISNVPGSEKSLYWNGARLQSLYPA
SIVFNGQAMNITLASYLDKIEFGITACSKALPHVQDMLMLIEEELQLLEKVSKELEFNGITVEDKSGYKGNGKTKKLAP

Specific function: Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS us

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20; =2.3.1.75 [H]

Molecular weight: Translated: 54536; Mature: 54536

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLTAVDQLFLLLESRKQPMHVGGLFLFELPEDADISFVHQLVKQMQDSHVPPTFPFNQ
CHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
VLEHMVFWKKDKNFDVEHHLHHVALPKPARVRELLMYVSREHGRLLDRAMPLWECHVIEG
HHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
IQPESEGSPERFALYFKIHHSLVDGIAAMRLVKKSLSQSPNEPVTLPIWSLMARHRNQID
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
AILPKERSALRILKEQVSTIKPVFTELLDNFKNYNDDSYVSTFDAPRSILNRRISASRRI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHH
AAQSYDIKRFNDIAERINISKNDVVLAVCAGAIRRYLISMDALPSKPLIAFVPMSLRTDD
HHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECCCC
SVAGNQLSFVLANLGTHLDDPLSRIKLIHRSMNNGKRRFRRMNQAQVINYSVVSYAWEGI
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHCCC
NLATGLFPKKQAFNLIISNVPGSEKSLYWNGARLQSLYPASIVFNGQAMNITLASYLDKI
HHHHCCCCCHHHHHHHEECCCCCCCEEEECCCHHCCCCCCEEEECCCEEEEEHHHHHHHH
EFGITACSKALPHVQDMLMLIEEELQLLEKVSKELEFNGITVEDKSGYKGNGKTKKLAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLLTAVDQLFLLLESRKQPMHVGGLFLFELPEDADISFVHQLVKQMQDSHVPPTFPFNQ
CHHHHHHHHHHHHHHHCCCCCEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
VLEHMVFWKKDKNFDVEHHLHHVALPKPARVRELLMYVSREHGRLLDRAMPLWECHVIEG
HHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
IQPESEGSPERFALYFKIHHSLVDGIAAMRLVKKSLSQSPNEPVTLPIWSLMARHRNQID
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
AILPKERSALRILKEQVSTIKPVFTELLDNFKNYNDDSYVSTFDAPRSILNRRISASRRI
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHH
AAQSYDIKRFNDIAERINISKNDVVLAVCAGAIRRYLISMDALPSKPLIAFVPMSLRTDD
HHHCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECCCC
SVAGNQLSFVLANLGTHLDDPLSRIKLIHRSMNNGKRRFRRMNQAQVINYSVVSYAWEGI
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHCCC
NLATGLFPKKQAFNLIISNVPGSEKSLYWNGARLQSLYPASIVFNGQAMNITLASYLDKI
HHHHCCCCCHHHHHHHEECCCCCCCEEEECCCHHCCCCCCEEEECCCEEEEEHHHHHHHH
EFGITACSKALPHVQDMLMLIEEELQLLEKVSKELEFNGITVEDKSGYKGNGKTKKLAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12502715 [H]