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The map label for this gene is 93005053

Identifier: 93005053

GI number: 93005053

Start: 266036

End: 266932

Strand: Reverse

Name: 93005053

Synonym: Pcryo_0222

Alternate gene names: NA

Gene position: 266932-266036 (Counterclockwise)

Preceding gene: 93005056

Following gene: 93005048

Centisome position: 8.72

GC content: 47.16

Gene sequence:

>897_bases
ATGAATGAACATATAAATAACATACAGCTGACCGTTGCTGCCATCCAAATGAATAGTCAGCAAAGCATTGAAGACAATCT
GTCAGATATAAAATCTGCTATTATAGATGCTCGTGCACAAGGCGCGCAGCTTGTCGTACTACCAGAAAACTGCTGTAGCA
TGGGTAGACAGTTTGCGACAGCAGAGCGCTTTGATGCGCTGTCAGCGACGATAGCAGAGTATGCACGTACATATGGTATA
TACGTGCTAGCAGGCTCTCTGCCCTGCCCGTATCGCCCTGATGGTATGATTGTGCCTGATGGTAGATTGCGCCAAGTAAG
CCTATTGTTTGCGCCGGATGGTACACAAGTGGCGCGCTATGACAAGATTCACTTATTCACCGCGACCGTCGCCGATAAGC
AAGGCAGTTATAACGAAGCGGCGACGTTTGAGCCAGGTACGCAGACGGTCGTGGCAGCGCTCGAAGTAAATGATGATATT
TATCAGCTCGGTATGATGGTTTGCTTTGATCTACGCTTTCCTGCATTGGCGCAGCGTCTGCGGCAAGCAGGCGCAGAACT
ATTAAGTGCGCCATCTGCTTTTACGTATCTAACAGGACAAGCCCATTGGTCGCTCTTACTGCAAGCGCGAGCTTTAGACA
GTCAATGTATGGTAATTGGAGCAGCACAAGGCGGTGAGCATGCGTATAAAGACGGTCATACACGTCAGACGTGGGGACAT
ACGACGATTAGCGCTTATGATGGAACTGTAATGAGCAGCTACAATAATAGCGAATTGACCCCATCTTTGAATAAAGACCA
TAAAAATTATGCTTTGGTCTTAGCGACGTTAGATAGACAAGCGCAAAATCAGGGACGACAAAAGATGCCTATCTTTGATT
GTCATCGTTTGGCATAA

Upstream 100 bases:

>100_bases
AATGACTTGCGGAGTAAGGTTCAAATAAACCATTAATAATGACAAACCTGAAAACGTTTTATAAGAGATATATAATATTA
ACAATATTGAGAAGCCTGCT

Downstream 100 bases:

>100_bases
AATAATTTTTATAAAAGCGTCTTGATAAAAGGTTTTTTTAATCAAAGCAATTTGCTATGAAAGACACTTTTAAAATTAAA
GGGTTGAATCGCTGTCTTTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNEHINNIQLTVAAIQMNSQQSIEDNLSDIKSAIIDARAQGAQLVVLPENCCSMGRQFATAERFDALSATIAEYARTYGI
YVLAGSLPCPYRPDGMIVPDGRLRQVSLLFAPDGTQVARYDKIHLFTATVADKQGSYNEAATFEPGTQTVVAALEVNDDI
YQLGMMVCFDLRFPALAQRLRQAGAELLSAPSAFTYLTGQAHWSLLLQARALDSQCMVIGAAQGGEHAYKDGHTRQTWGH
TTISAYDGTVMSSYNNSELTPSLNKDHKNYALVLATLDRQAQNQGRQKMPIFDCHRLA

Sequences:

>Translated_298_residues
MNEHINNIQLTVAAIQMNSQQSIEDNLSDIKSAIIDARAQGAQLVVLPENCCSMGRQFATAERFDALSATIAEYARTYGI
YVLAGSLPCPYRPDGMIVPDGRLRQVSLLFAPDGTQVARYDKIHLFTATVADKQGSYNEAATFEPGTQTVVAALEVNDDI
YQLGMMVCFDLRFPALAQRLRQAGAELLSAPSAFTYLTGQAHWSLLLQARALDSQCMVIGAAQGGEHAYKDGHTRQTWGH
TTISAYDGTVMSSYNNSELTPSLNKDHKNYALVLATLDRQAQNQGRQKMPIFDCHRLA
>Mature_298_residues
MNEHINNIQLTVAAIQMNSQQSIEDNLSDIKSAIIDARAQGAQLVVLPENCCSMGRQFATAERFDALSATIAEYARTYGI
YVLAGSLPCPYRPDGMIVPDGRLRQVSLLFAPDGTQVARYDKIHLFTATVADKQGSYNEAATFEPGTQTVVAALEVNDDI
YQLGMMVCFDLRFPALAQRLRQAGAELLSAPSAFTYLTGQAHWSLLLQARALDSQCMVIGAAQGGEHAYKDGHTRQTWGH
TTISAYDGTVMSSYNNSELTPSLNKDHKNYALVLATLDRQAQNQGRQKMPIFDCHRLA

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=297, Percent_Identity=32.3232323232323, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI297632350, Length=293, Percent_Identity=31.3993174061433, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI5031947, Length=293, Percent_Identity=31.3993174061433, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=31.1643835616438, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI297632346, Length=200, Percent_Identity=31, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=248, Percent_Identity=34.2741935483871, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6322335, Length=310, Percent_Identity=30.3225806451613, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6323383, Length=304, Percent_Identity=24.0131578947368, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17933642, Length=256, Percent_Identity=35.9375, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355835, Length=285, Percent_Identity=24.9122807017544, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: NA

Molecular weight: Translated: 32696; Mature: 32696

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEHINNIQLTVAAIQMNSQQSIEDNLSDIKSAIIDARAQGAQLVVLPENCCSMGRQFAT
CCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHH
AERFDALSATIAEYARTYGIYVLAGSLPCPYRPDGMIVPDGRLRQVSLLFAPDGTQVARY
HHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCCCCEEEEEEEECCCCCCEEEE
DKIHLFTATVADKQGSYNEAATFEPGTQTVVAALEVNDDIYQLGMMVCFDLRFPALAQRL
EEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHH
RQAGAELLSAPSAFTYLTGQAHWSLLLQARALDSQCMVIGAAQGGEHAYKDGHTRQTWGH
HHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCC
TTISAYDGTVMSSYNNSELTPSLNKDHKNYALVLATLDRQAQNQGRQKMPIFDCHRLA
EEEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHCC
>Mature Secondary Structure
MNEHINNIQLTVAAIQMNSQQSIEDNLSDIKSAIIDARAQGAQLVVLPENCCSMGRQFAT
CCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHH
AERFDALSATIAEYARTYGIYVLAGSLPCPYRPDGMIVPDGRLRQVSLLFAPDGTQVARY
HHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCEECCCCCEEEEEEEECCCCCCEEEE
DKIHLFTATVADKQGSYNEAATFEPGTQTVVAALEVNDDIYQLGMMVCFDLRFPALAQRL
EEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHH
RQAGAELLSAPSAFTYLTGQAHWSLLLQARALDSQCMVIGAAQGGEHAYKDGHTRQTWGH
HHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCC
TTISAYDGTVMSSYNNSELTPSLNKDHKNYALVLATLDRQAQNQGRQKMPIFDCHRLA
EEEEECCCEEEECCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]