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The map label for this gene is nasC [H]

Identifier: 93004998

GI number: 93004998

Start: 195714

End: 197939

Strand: Direct

Name: nasC [H]

Synonym: Pcryo_0167

Alternate gene names: 93004998

Gene position: 195714-197939 (Clockwise)

Preceding gene: 93004994

Following gene: 93005001

Centisome position: 6.4

GC content: 45.01

Gene sequence:

>2226_bases
GTGGCGACTGAACAAACCAAATCCAAAGAGCAGACGCATTTTAGAACCTGTACTCTGTGTGAGGCAATGTGTGGTATCGA
GATAAAGCACGATGGCAAAAAGGTTTTATCGATTAAAGGGGATAAGAGCGATCCATTTTCTAAAGGGTATATTTGCCCAA
AAGCGACTGCCTTGCAAGATTTGCATGAGGATACTGACCGCCTGCGTTATCCTGTTGAAAGAACGGCTAAAGGTTGGAAA
GAAATTAGCTGGACTGAAGCGTTAGATAAAGTCGCAGCTGGTATTGGCGCAGTGCAAAAACAATATGGCAAAAATGCTTT
TGGTGTTTATTTGGGCAATCCCAACGTACACAATCTGGGCGGTATGCTGACAATCAAGTATCTGCTAAACAGCCTCAAAA
CGCGCAGTCGCTTCTCTGCCACTTCAGTCGATCAATTACCGCACCATATCGTTAGCATGCATTTATTTGGGCATATGCTG
CGTATTCCAGTACCTGATGTCAACCGTACTAATTATATGCTCATCATCGGTGGCAATCCGCTAGCGTCTAACGGCAGTAT
TATGACGGCACCAAATATGCGCCAAAAACTAAAAGACATTAAAGCACGTGGTGGCAAAGTCGTCGTCATCGATCCAAGAC
GTACCGAAACCGCTGATATCGCAAGCGAGCATCACTTTATTCGTCCAGCGACTGACGTGTTGCTATTATTAGCGATGCTC
AATGAGATTTATTTGCAAGGCTATGCTGATAAAGCAAGTATCAATAATAGAGCAGCAGCACTCGCGCCAGAAATAGAGCG
ACTGGCAGATTTTGCCAAAGACTATAGTGCAGAGTCAGTTGCCGCTATTACGGGCATAGCTGCGGTTGACATCAAGCGAC
TGGTCACAGAATTTTGTGAAGCAAAAAGCTCGGTTTGCTATGGTCGGATGGGTATATCAGTGCAGGAATTCGGTTTATTA
AGCCAGTATTTGTCTATGGTTATTAATATCGTGACGGGGCGCTTGGACGAAGTGGGTGGTTTGATGTTCCCGAATGCTGC
AGTCGATGTGGTGAATAATTCTGGTCCGGGGTATTTGGGTAAGCGTCATAGTCGAGTCAGCAAGCTACCTGACTTTAATG
GTGAATATCCCGTGGTTGCCATGAGTGATGAGATGTTGATCGAAGGTGAAGGGCAATTAAAAGGCTTTATGACGGTAGCG
GGTAATCCTGTCTTGAGTACACCCAATGGCGAAAAATTAGATCAAGCCTTTGAACAGTTAGATTTTATGGTCGCCATTGA
TTATTTTGTTAATGAAACCAGTCGCCATGCTAATATTATTTTGCCACCAGTCTCACCGCTAGAGCGCGACCATTACGACG
TTACCTTTAATAATTTTGCCGTACATAACGTCGCTAAATATTCAAAAGCGTTGTTTCGTAAAGATAAGGACGCCAAGCAT
GATTGGCAAATCTATCTAGAGCTGGCAAAGCGCTTGGATAAAAAAGCGTCTTTAGCAACCAAAGTCGAACGGTTGTTAAT
AAAGAGATTAGGACCGAAATTCATACTCGATCAAGGATTGCGGCGTGGACCTTATGCTGGCATGACACTGAACAAGCTCA
AGAAAAATGCTCATGGACTTGATTTAGGACCGCTTAAAACCATGTTGCCGCAAGCTTTACGACACAAAGATAAGCAGATT
CATCTGAATGTCGATTTTTACCAAGCAGATCTTGCCCGCGTGCAAGCGATGATGCAAAATTATGATGACAGCGAGATTTT
ATTGATAGGTCGTCGTCATGTCCGCAGTAATAATTCGTGGCTGCATAACAGCTATCGCTTGGTCAAAGGTAAGCCGCGCT
GTACGCTCATGCTGCATCCAGATACTGCTGCGCAATATGGTATTGAGGATGGTCAAGACGTGAAAGTCACCTCGCGCGTC
GGTAGCGTGATTGTTGCAGCAGAAATCACTGATGAGTTGATGGCGGGGGTAGTGAGTATTCCGCATGGCTTTGGTCACGG
CCGTAAAGGCGTTAAGCAAAAAATCGCCCAAGCGCATGCAGGTGTCAGTGTGAATGATCTGACAGATGATACGTTGATTG
ATGAGTTAAGCGGTAATGCCGCTGTAAATGGCGTCCCTGTGCAATTGGAAGCTATACATGCAGGAACTATATTCTCAGAA
GCTGCTGAGTCAAACAGTTCGAACTCTTCGTCACATTCAAAAGCCGAGTCGGGTAGCGCTGCATAA

Upstream 100 bases:

>100_bases
AGCGAGATATAGTAGAGAATAGACTGTCAGGAATCAAAGTCTTAAAAGGAATTCAGTATCTATTTATTGAGAACGACAAT
ATAAAACAGGGAGCGTGACC

Downstream 100 bases:

>100_bases
ATACAATCAATCCTATAGTTAAGATTGGACTCGAAAGCGCTTATTTATAATGATAAGCGCTTTTTATTTACCTTATTAAT
TTGTCTTAGACTAAATCTAG

Product: molydopterin dinucleotide-binding region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 741; Mature: 740

Protein sequence:

>741_residues
MATEQTKSKEQTHFRTCTLCEAMCGIEIKHDGKKVLSIKGDKSDPFSKGYICPKATALQDLHEDTDRLRYPVERTAKGWK
EISWTEALDKVAAGIGAVQKQYGKNAFGVYLGNPNVHNLGGMLTIKYLLNSLKTRSRFSATSVDQLPHHIVSMHLFGHML
RIPVPDVNRTNYMLIIGGNPLASNGSIMTAPNMRQKLKDIKARGGKVVVIDPRRTETADIASEHHFIRPATDVLLLLAML
NEIYLQGYADKASINNRAAALAPEIERLADFAKDYSAESVAAITGIAAVDIKRLVTEFCEAKSSVCYGRMGISVQEFGLL
SQYLSMVINIVTGRLDEVGGLMFPNAAVDVVNNSGPGYLGKRHSRVSKLPDFNGEYPVVAMSDEMLIEGEGQLKGFMTVA
GNPVLSTPNGEKLDQAFEQLDFMVAIDYFVNETSRHANIILPPVSPLERDHYDVTFNNFAVHNVAKYSKALFRKDKDAKH
DWQIYLELAKRLDKKASLATKVERLLIKRLGPKFILDQGLRRGPYAGMTLNKLKKNAHGLDLGPLKTMLPQALRHKDKQI
HLNVDFYQADLARVQAMMQNYDDSEILLIGRRHVRSNNSWLHNSYRLVKGKPRCTLMLHPDTAAQYGIEDGQDVKVTSRV
GSVIVAAEITDELMAGVVSIPHGFGHGRKGVKQKIAQAHAGVSVNDLTDDTLIDELSGNAAVNGVPVQLEAIHAGTIFSE
AAESNSSNSSSHSKAESGSAA

Sequences:

>Translated_741_residues
MATEQTKSKEQTHFRTCTLCEAMCGIEIKHDGKKVLSIKGDKSDPFSKGYICPKATALQDLHEDTDRLRYPVERTAKGWK
EISWTEALDKVAAGIGAVQKQYGKNAFGVYLGNPNVHNLGGMLTIKYLLNSLKTRSRFSATSVDQLPHHIVSMHLFGHML
RIPVPDVNRTNYMLIIGGNPLASNGSIMTAPNMRQKLKDIKARGGKVVVIDPRRTETADIASEHHFIRPATDVLLLLAML
NEIYLQGYADKASINNRAAALAPEIERLADFAKDYSAESVAAITGIAAVDIKRLVTEFCEAKSSVCYGRMGISVQEFGLL
SQYLSMVINIVTGRLDEVGGLMFPNAAVDVVNNSGPGYLGKRHSRVSKLPDFNGEYPVVAMSDEMLIEGEGQLKGFMTVA
GNPVLSTPNGEKLDQAFEQLDFMVAIDYFVNETSRHANIILPPVSPLERDHYDVTFNNFAVHNVAKYSKALFRKDKDAKH
DWQIYLELAKRLDKKASLATKVERLLIKRLGPKFILDQGLRRGPYAGMTLNKLKKNAHGLDLGPLKTMLPQALRHKDKQI
HLNVDFYQADLARVQAMMQNYDDSEILLIGRRHVRSNNSWLHNSYRLVKGKPRCTLMLHPDTAAQYGIEDGQDVKVTSRV
GSVIVAAEITDELMAGVVSIPHGFGHGRKGVKQKIAQAHAGVSVNDLTDDTLIDELSGNAAVNGVPVQLEAIHAGTIFSE
AAESNSSNSSSHSKAESGSAA
>Mature_740_residues
ATEQTKSKEQTHFRTCTLCEAMCGIEIKHDGKKVLSIKGDKSDPFSKGYICPKATALQDLHEDTDRLRYPVERTAKGWKE
ISWTEALDKVAAGIGAVQKQYGKNAFGVYLGNPNVHNLGGMLTIKYLLNSLKTRSRFSATSVDQLPHHIVSMHLFGHMLR
IPVPDVNRTNYMLIIGGNPLASNGSIMTAPNMRQKLKDIKARGGKVVVIDPRRTETADIASEHHFIRPATDVLLLLAMLN
EIYLQGYADKASINNRAAALAPEIERLADFAKDYSAESVAAITGIAAVDIKRLVTEFCEAKSSVCYGRMGISVQEFGLLS
QYLSMVINIVTGRLDEVGGLMFPNAAVDVVNNSGPGYLGKRHSRVSKLPDFNGEYPVVAMSDEMLIEGEGQLKGFMTVAG
NPVLSTPNGEKLDQAFEQLDFMVAIDYFVNETSRHANIILPPVSPLERDHYDVTFNNFAVHNVAKYSKALFRKDKDAKHD
WQIYLELAKRLDKKASLATKVERLLIKRLGPKFILDQGLRRGPYAGMTLNKLKKNAHGLDLGPLKTMLPQALRHKDKQIH
LNVDFYQADLARVQAMMQNYDDSEILLIGRRHVRSNNSWLHNSYRLVKGKPRCTLMLHPDTAAQYGIEDGQDVKVTSRVG
SVIVAAEITDELMAGVVSIPHGFGHGRKGVKQKIAQAHAGVSVNDLTDDTLIDELSGNAAVNGVPVQLEAIHAGTIFSEA
AESNSSNSSSHSKAESGSAA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=692, Percent_Identity=22.9768786127168, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787870, Length=780, Percent_Identity=22.0512820512821, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI3868719, Length=246, Percent_Identity=28.8617886178862, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI171474008, Length=745, Percent_Identity=22.0134228187919, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 81464; Mature: 81333

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEQTKSKEQTHFRTCTLCEAMCGIEIKHDGKKVLSIKGDKSDPFSKGYICPKATALQD
CCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
LHEDTDRLRYPVERTAKGWKEISWTEALDKVAAGIGAVQKQYGKNAFGVYLGNPNVHNLG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
GMLTIKYLLNSLKTRSRFSATSVDQLPHHIVSMHLFGHMLRIPVPDVNRTNYMLIIGGNP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LASNGSIMTAPNMRQKLKDIKARGGKVVVIDPRRTETADIASEHHFIRPATDVLLLLAML
CCCCCCEEECCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
NEIYLQGYADKASINNRAAALAPEIERLADFAKDYSAESVAAITGIAAVDIKRLVTEFCE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AKSSVCYGRMGISVQEFGLLSQYLSMVINIVTGRLDEVGGLMFPNAAVDVVNNSGPGYLG
HHCCCEEHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCEECCCCCEEEEECCCCCCCC
KRHSRVSKLPDFNGEYPVVAMSDEMLIEGEGQLKGFMTVAGNPVLSTPNGEKLDQAFEQL
CHHHHHHHCCCCCCCCCEEEECCCEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHHH
DFMVAIDYFVNETSRHANIILPPVSPLERDHYDVTFNNFAVHNVAKYSKALFRKDKDAKH
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCH
DWQIYLELAKRLDKKASLATKVERLLIKRLGPKFILDQGLRRGPYAGMTLNKLKKNAHGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCC
DLGPLKTMLPQALRHKDKQIHLNVDFYQADLARVQAMMQNYDDSEILLIGRRHVRSNNSW
CCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCE
LHNSYRLVKGKPRCTLMLHPDTAAQYGIEDGQDVKVTSRVGSVIVAAEITDELMAGVVSI
EECCEEEEECCCCEEEEECCCCHHHCCCCCCCCEEEEHHHCCEEEEHHHHHHHHHHHHHC
PHGFGHGRKGVKQKIAQAHAGVSVNDLTDDTLIDELSGNAAVNGVPVQLEAIHAGTIFSE
CCCCCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCEECCCEEEEEEEECCHHHHH
AAESNSSNSSSHSKAESGSAA
HHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATEQTKSKEQTHFRTCTLCEAMCGIEIKHDGKKVLSIKGDKSDPFSKGYICPKATALQD
CCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
LHEDTDRLRYPVERTAKGWKEISWTEALDKVAAGIGAVQKQYGKNAFGVYLGNPNVHNLG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
GMLTIKYLLNSLKTRSRFSATSVDQLPHHIVSMHLFGHMLRIPVPDVNRTNYMLIIGGNP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LASNGSIMTAPNMRQKLKDIKARGGKVVVIDPRRTETADIASEHHFIRPATDVLLLLAML
CCCCCCEEECCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHH
NEIYLQGYADKASINNRAAALAPEIERLADFAKDYSAESVAAITGIAAVDIKRLVTEFCE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AKSSVCYGRMGISVQEFGLLSQYLSMVINIVTGRLDEVGGLMFPNAAVDVVNNSGPGYLG
HHCCCEEHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCEECCCCCEEEEECCCCCCCC
KRHSRVSKLPDFNGEYPVVAMSDEMLIEGEGQLKGFMTVAGNPVLSTPNGEKLDQAFEQL
CHHHHHHHCCCCCCCCCEEEECCCEEEECCCCEEEEEEECCCEEEECCCCHHHHHHHHHH
DFMVAIDYFVNETSRHANIILPPVSPLERDHYDVTFNNFAVHNVAKYSKALFRKDKDAKH
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCH
DWQIYLELAKRLDKKASLATKVERLLIKRLGPKFILDQGLRRGPYAGMTLNKLKKNAHGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCCCC
DLGPLKTMLPQALRHKDKQIHLNVDFYQADLARVQAMMQNYDDSEILLIGRRHVRSNNSW
CCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECHHHCCCCCE
LHNSYRLVKGKPRCTLMLHPDTAAQYGIEDGQDVKVTSRVGSVIVAAEITDELMAGVVSI
EECCEEEEECCCCEEEEECCCCHHHCCCCCCCCEEEEHHHCCEEEEHHHHHHHHHHHHHC
PHGFGHGRKGVKQKIAQAHAGVSVNDLTDDTLIDELSGNAAVNGVPVQLEAIHAGTIFSE
CCCCCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCEECCCEEEEEEEECCHHHHH
AAESNSSNSSSHSKAESGSAA
HHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]