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The map label for this gene is prfC [H]

Identifier: 92113951

GI number: 92113951

Start: 2083406

End: 2085040

Strand: Direct

Name: prfC [H]

Synonym: Csal_1828

Alternate gene names: 92113951

Gene position: 2083406-2085040 (Clockwise)

Preceding gene: 92113948

Following gene: 92113952

Centisome position: 56.36

GC content: 59.63

Gene sequence:

>1635_bases
ATGCATTTTCTGCGTCGAGCTGCCGCCAATCAGCGAAGTATATTGATGCCAGACACTCAGATCGCTCAAGAAGCGGGGCT
ACGCAGAACCTTTGCGATCATTTCGCACCCCGACGCCGGCAAGACCACCATCACGGAAAAAATGCTGCTGTTCGGCAATG
CGATCCAGCTAGCCGGGTCGGTCAAGAGCAAGCGTGACGAACGTCACGCCACCTCCGACTGGATGAAGATGGAGCAGGAG
CGTGGCATCTCGGTGACGACCTCGGTCATGCAGTTTCCCTACCAGGGCCGGATCGTCAATCTGCTCGACACGCCCGGGCA
CGAAGACTTTTCCGAGGACACCTACCGCACGCTGACGGCCGTGGATTCGGCACTGATGGTCATCGACGGCGCCAAGGGGG
TCGAAGATCGTACCATCAAGCTCATGGATGTGTGTCGTTTGCGTACGACGCCGATCCTGACCTTCATCAACAAGATGGAC
CGAGACATCCGGGATCCGATCGAAGTGATGGATGAGGTCGAGACGGTACTGAACATTCAGTGTGCGCCCATGACGTGGCC
GATTGGCATGGGGCGGCATTTTCGCGGCGTCTACCATCTCTACAACGATGTCATTCACCTCTACAAGCAGGGGCAGGGCA
ATCGCATTCCCGAGGATGTGCGCATCGAAGGTCTCGACAACCCCGAGGTCGACGAGACGCTGGGAGCGGATGCCGCTGAC
GAGCTGCGCATGGAAGTCGAGCTGGTGCGTGGTGCCTCGCATACCTTCGACCTCGAGGCCTATCGTCGCGGTGAACTGAC
GCCAGTGTATTTCGGCACCGCCATGGGCAACTTCGGGGTGCGCGAGATGCTCGATGGCTTCGTCGAATACGCGCCGGCGC
CGTTGCCGCGCGACACCGACAAGCGCAGCGTAAAGGCCGATGACGAGCGCTTTACCGGTTTCGTCTTCAAGATCCAGGCC
AACATGGATCCCAAGCACCGTGACCGTGTTGCCTTCTTGCGCGTCTGTTCCGGGAAGTACGACAAGAACATGAAGATGCG
CCACGTGCGGATCGGCAAAGACGTCAAGATTGCCGACGCGCTAACGTTCATGGCGTCCGATCGCTCGCATGTCGAGGAAG
CCTGGCCCGGCGATATCATCGGCCTGCACAACCATGGCACGATCCAGATCGGTGACACCTTCACGCTCAACGAGGACATG
CGCTTCACGGGAATTCCGCACTTCGCGCCCGAGTTGTTCAAGCGCGTGCGTCTGCGCGATCCGCTGAAGATGAAGGCCTT
GCAGAAAGGGCTGACCCAGCTCTCCGAGGAGGGGGCAACGCAGGTCTTCATGCCGCTGGATAACAATGACCTGATCGTCG
GCGCCGTGGGGTCGTTGCAGTTCGATGTGGTCGCTCATCGCCTGAAGCAGGAATATAAAGTCGACTGCCTGTATGAGCCC
GTGAACGTGCAGACGGCGCGCTGGGTGTATTGCAACGACGCCAAGATGCTCGACGAATTCAAGCGCAAGGCCAGCACCAA
CCTGGCCCTGGACGGCGGGGGATACCTGACGTATCTGGCCCCGACGCGCGTCAACCTGCAGATGACGCAAGAGCGTTGGC
CGGATGTCGAGTTCCGTGCCACCCGGGAGCATTGA

Upstream 100 bases:

>100_bases
GGGCGGGTGGTGCTGTGCCCCACGAGGTCAGGGCGCAAGGCCTACCCTCATGCGCGTCTAAGCAGTACAATTTGCGCCCA
GTTACCATTCTCTCGGCTCG

Downstream 100 bases:

>100_bases
CGCAACGGCTTGACCGCCCGCTTTCCGCGTCTCGGAGCGACACGTCTTGCGCCAGACGCCATGGCGTGCCGCCAGGATAG
CTGCAAGGGAGGGAATCATG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MHFLRRAAANQRSILMPDTQIAQEAGLRRTFAIISHPDAGKTTITEKMLLFGNAIQLAGSVKSKRDERHATSDWMKMEQE
RGISVTTSVMQFPYQGRIVNLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEDRTIKLMDVCRLRTTPILTFINKMD
RDIRDPIEVMDEVETVLNIQCAPMTWPIGMGRHFRGVYHLYNDVIHLYKQGQGNRIPEDVRIEGLDNPEVDETLGADAAD
ELRMEVELVRGASHTFDLEAYRRGELTPVYFGTAMGNFGVREMLDGFVEYAPAPLPRDTDKRSVKADDERFTGFVFKIQA
NMDPKHRDRVAFLRVCSGKYDKNMKMRHVRIGKDVKIADALTFMASDRSHVEEAWPGDIIGLHNHGTIQIGDTFTLNEDM
RFTGIPHFAPELFKRVRLRDPLKMKALQKGLTQLSEEGATQVFMPLDNNDLIVGAVGSLQFDVVAHRLKQEYKVDCLYEP
VNVQTARWVYCNDAKMLDEFKRKASTNLALDGGGYLTYLAPTRVNLQMTQERWPDVEFRATREH

Sequences:

>Translated_544_residues
MHFLRRAAANQRSILMPDTQIAQEAGLRRTFAIISHPDAGKTTITEKMLLFGNAIQLAGSVKSKRDERHATSDWMKMEQE
RGISVTTSVMQFPYQGRIVNLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEDRTIKLMDVCRLRTTPILTFINKMD
RDIRDPIEVMDEVETVLNIQCAPMTWPIGMGRHFRGVYHLYNDVIHLYKQGQGNRIPEDVRIEGLDNPEVDETLGADAAD
ELRMEVELVRGASHTFDLEAYRRGELTPVYFGTAMGNFGVREMLDGFVEYAPAPLPRDTDKRSVKADDERFTGFVFKIQA
NMDPKHRDRVAFLRVCSGKYDKNMKMRHVRIGKDVKIADALTFMASDRSHVEEAWPGDIIGLHNHGTIQIGDTFTLNEDM
RFTGIPHFAPELFKRVRLRDPLKMKALQKGLTQLSEEGATQVFMPLDNNDLIVGAVGSLQFDVVAHRLKQEYKVDCLYEP
VNVQTARWVYCNDAKMLDEFKRKASTNLALDGGGYLTYLAPTRVNLQMTQERWPDVEFRATREH
>Mature_544_residues
MHFLRRAAANQRSILMPDTQIAQEAGLRRTFAIISHPDAGKTTITEKMLLFGNAIQLAGSVKSKRDERHATSDWMKMEQE
RGISVTTSVMQFPYQGRIVNLLDTPGHEDFSEDTYRTLTAVDSALMVIDGAKGVEDRTIKLMDVCRLRTTPILTFINKMD
RDIRDPIEVMDEVETVLNIQCAPMTWPIGMGRHFRGVYHLYNDVIHLYKQGQGNRIPEDVRIEGLDNPEVDETLGADAAD
ELRMEVELVRGASHTFDLEAYRRGELTPVYFGTAMGNFGVREMLDGFVEYAPAPLPRDTDKRSVKADDERFTGFVFKIQA
NMDPKHRDRVAFLRVCSGKYDKNMKMRHVRIGKDVKIADALTFMASDRSHVEEAWPGDIIGLHNHGTIQIGDTFTLNEDM
RFTGIPHFAPELFKRVRLRDPLKMKALQKGLTQLSEEGATQVFMPLDNNDLIVGAVGSLQFDVVAHRLKQEYKVDCLYEP
VNVQTARWVYCNDAKMLDEFKRKASTNLALDGGGYLTYLAPTRVNLQMTQERWPDVEFRATREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=27.5933609958506, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI19923640, Length=526, Percent_Identity=24.3346007604563, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI25306283, Length=392, Percent_Identity=26.7857142857143, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI25306287, Length=498, Percent_Identity=23.6947791164659, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI157426893, Length=168, Percent_Identity=29.7619047619048, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=32, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=70.1149425287356, Blast_Score=774, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=497, Percent_Identity=28.7726358148893, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=32.6923076923077, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994988, Length=175, Percent_Identity=29.7142857142857, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17533571, Length=547, Percent_Identity=27.9707495429616, Blast_Score=186, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17556745, Length=473, Percent_Identity=24.5243128964059, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=31.8181818181818, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=477, Percent_Identity=28.5115303983228, Blast_Score=170, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=510, Percent_Identity=24.5098039215686, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.1655629139073, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=30, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=30, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24582462, Length=473, Percent_Identity=27.906976744186, Blast_Score=171, Evalue=9e-43,
Organism=Drosophila melanogaster, GI221458488, Length=507, Percent_Identity=25.2465483234714, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI78706572, Length=155, Percent_Identity=30.9677419354839, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=30.5194805194805, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=30.5194805194805, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=30.5194805194805, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 61923; Mature: 61923

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFLRRAAANQRSILMPDTQIAQEAGLRRTFAIISHPDAGKTTITEKMLLFGNAIQLAGS
CCHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHCC
VKSKRDERHATSDWMKMEQERGISVTTSVMQFPYQGRIVNLLDTPGHEDFSEDTYRTLTA
HHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
VDSALMVIDGAKGVEDRTIKLMDVCRLRTTPILTFINKMDRDIRDPIEVMDEVETVLNIQ
HCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHEEE
CAPMTWPIGMGRHFRGVYHLYNDVIHLYKQGQGNRIPEDVRIEGLDNPEVDETLGADAAD
ECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHCCCCHH
ELRMEVELVRGASHTFDLEAYRRGELTPVYFGTAMGNFGVREMLDGFVEYAPAPLPRDTD
HHHHHHHHHHCCCCEEEHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
KRSVKADDERFTGFVFKIQANMDPKHRDRVAFLRVCSGKYDKNMKMRHVRIGKDVKIADA
CCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEHHHH
LTFMASDRSHVEEAWPGDIIGLHNHGTIQIGDTFTLNEDMRFTGIPHFAPELFKRVRLRD
HHHHHCCHHHHHHCCCCCEEEECCCCEEEECCEEEECCCCEECCCCHHHHHHHHHHHCCC
PLKMKALQKGLTQLSEEGATQVFMPLDNNDLIVGAVGSLQFDVVAHRLKQEYKVDCLYEP
CHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECC
VNVQTARWVYCNDAKMLDEFKRKASTNLALDGGGYLTYLAPTRVNLQMTQERWPDVEFRA
CCCCEEEEEEECCHHHHHHHHHHHCCCEEECCCCEEEEECCCEEEEEEHHHHCCCCEEEE
TREH
CCCC
>Mature Secondary Structure
MHFLRRAAANQRSILMPDTQIAQEAGLRRTFAIISHPDAGKTTITEKMLLFGNAIQLAGS
CCHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHCC
VKSKRDERHATSDWMKMEQERGISVTTSVMQFPYQGRIVNLLDTPGHEDFSEDTYRTLTA
HHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHH
VDSALMVIDGAKGVEDRTIKLMDVCRLRTTPILTFINKMDRDIRDPIEVMDEVETVLNIQ
HCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHEEE
CAPMTWPIGMGRHFRGVYHLYNDVIHLYKQGQGNRIPEDVRIEGLDNPEVDETLGADAAD
ECCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCHHHHHCCCCHH
ELRMEVELVRGASHTFDLEAYRRGELTPVYFGTAMGNFGVREMLDGFVEYAPAPLPRDTD
HHHHHHHHHHCCCCEEEHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
KRSVKADDERFTGFVFKIQANMDPKHRDRVAFLRVCSGKYDKNMKMRHVRIGKDVKIADA
CCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEHHHH
LTFMASDRSHVEEAWPGDIIGLHNHGTIQIGDTFTLNEDMRFTGIPHFAPELFKRVRLRD
HHHHHCCHHHHHHCCCCCEEEECCCCEEEECCEEEECCCCEECCCCHHHHHHHHHHHCCC
PLKMKALQKGLTQLSEEGATQVFMPLDNNDLIVGAVGSLQFDVVAHRLKQEYKVDCLYEP
CHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECC
VNVQTARWVYCNDAKMLDEFKRKASTNLALDGGGYLTYLAPTRVNLQMTQERWPDVEFRA
CCCCEEEEEEECCHHHHHHHHHHHCCCEEECCCCEEEEECCCEEEEEEHHHHCCCCEEEE
TREH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA