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The map label for this gene is livK [C]

Identifier: 92113848

GI number: 92113848

Start: 1965799

End: 1966980

Strand: Direct

Name: livK [C]

Synonym: Csal_1725

Alternate gene names: 92113848

Gene position: 1965799-1966980 (Clockwise)

Preceding gene: 92113847

Following gene: 92113849

Centisome position: 53.18

GC content: 67.68

Gene sequence:

>1182_bases
ATGAGGGTTCAACGCAGGTGGCTGGCAGGTGCATTGGCGCTTGCCCTAGTCGCCATGCCGGCCTGGGCGGAGATCAAGGT
CGGGATGGTGGCTCCATTCAGTGGGGCCGCATCGTCATTGGGCGAAGGCATGAAGCGGGGCATCGAGGCGCGTTTCGCCG
AGGTCAACGAGGCAGGGGGCGTGCATGGCGAATCGCTGACCCTGGTGACGCGCGACGACGGCTACGAGCCGCTGCGCTCG
GCCAAGGCGACACGCGAGCTGCTGGACGACGACGAGGTGCTGGCCATGCTGGGCAACGTCGGCACGCCCACGGCCATCGT
CAACCTGCCGATCCTTCTGGAGCACGAGATGCCGCTGATCGGGGCGCTCACCGGCAGCGGCGTGCTGCGTCAGTCTCCAC
CGGATCGTTACGTCTTCAATTATCGCGCCAGCTATGCCCAGGAAACCGCGACGATGATCGAGGGCCTGCTGCTGGCGGGC
ATCGCGCCCGAGGAAATCGCGTTCTTCACCCAGGCCGACGGCTATGGCGATGCCGGCTACCGGGGCGCACTGCGTTCCCT
GGCCAACCATGGGTATTCCCACCCCGAGCGTCTGGCCCACGGCCGCTATACCCGGGGCACGCGCAACGTGCATCGGGGGG
TGGCCACCATCCTGCAGGCGCCGGTCACGCCCAAGGCGGTGATCATCGTGGGCACCTACGGGCCGGCGGCGGATTTCATC
CGCGAGGCACGCAAGGACCTGCCCGACGCGCTGTTTCTCAACGTTTCCTTCGTGGGCAGTCAGGCGCTGCTCGACGAGCT
GGGGGACGCCGCCGAGGGCGTGATCGTCACCCAGGTGGTGCCGCCGCATACCTCGGATCTGCCGGGTGTCGAGGCGTATC
GCGCCGCCCTGCAGGCCAATGGTGCCGACAGCGATGGCGATTTCGTGTCGCTGGAGGGCTACATGGCAGCCTCGCTGTTC
GTCGAAGGACTCCAGGCGGCGGGCGCCGACCCCGACCGCGAGGCGCTCGTCGACGGGCTCGAGGGCCTCGGCAAAGTCGA
TCTCGGCCTGGGCGAGCCTCTGACGCTGGGGCCGGAGGATCATCAGGCAAACGATGCCGTGTGGGCGACACGGATTCATG
ACGGCCGCTTCGAGCCCGTCGATTGGTTCACCCTGCTCGATGAGCGCGCGGCCGAGGAGTAA

Upstream 100 bases:

>100_bases
AGTGGTTCCAACGGCGTCGCGGGTTCTCCCCGCGACAGCGCGAATCACGTCAAAGCGCGGCTAACGATAACGCGCGAATC
ACGATAAAGAGGCAGGGATC

Downstream 100 bases:

>100_bases
TCCCATGAAGAAGAGTTTGAGCCTGCGGGCGATGCTGATCGGGCTGTTCGTGGCCGCCGTGCTGGGGGCGTTGATTCCGG
CCGCGGCGGGATTTCTCTCC

Product: putative leucine/isoleucine/valine-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: Serine/Threonine Protein Kinase; Branched-Chain Amino Acid ABC Transporter; Lipoprotein; Signal Peptide; ABC/TRAP Transporter Component

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRVQRRWLAGALALALVAMPAWAEIKVGMVAPFSGAASSLGEGMKRGIEARFAEVNEAGGVHGESLTLVTRDDGYEPLRS
AKATRELLDDDEVLAMLGNVGTPTAIVNLPILLEHEMPLIGALTGSGVLRQSPPDRYVFNYRASYAQETATMIEGLLLAG
IAPEEIAFFTQADGYGDAGYRGALRSLANHGYSHPERLAHGRYTRGTRNVHRGVATILQAPVTPKAVIIVGTYGPAADFI
REARKDLPDALFLNVSFVGSQALLDELGDAAEGVIVTQVVPPHTSDLPGVEAYRAALQANGADSDGDFVSLEGYMAASLF
VEGLQAAGADPDREALVDGLEGLGKVDLGLGEPLTLGPEDHQANDAVWATRIHDGRFEPVDWFTLLDERAAEE

Sequences:

>Translated_393_residues
MRVQRRWLAGALALALVAMPAWAEIKVGMVAPFSGAASSLGEGMKRGIEARFAEVNEAGGVHGESLTLVTRDDGYEPLRS
AKATRELLDDDEVLAMLGNVGTPTAIVNLPILLEHEMPLIGALTGSGVLRQSPPDRYVFNYRASYAQETATMIEGLLLAG
IAPEEIAFFTQADGYGDAGYRGALRSLANHGYSHPERLAHGRYTRGTRNVHRGVATILQAPVTPKAVIIVGTYGPAADFI
REARKDLPDALFLNVSFVGSQALLDELGDAAEGVIVTQVVPPHTSDLPGVEAYRAALQANGADSDGDFVSLEGYMAASLF
VEGLQAAGADPDREALVDGLEGLGKVDLGLGEPLTLGPEDHQANDAVWATRIHDGRFEPVDWFTLLDERAAEE
>Mature_393_residues
MRVQRRWLAGALALALVAMPAWAEIKVGMVAPFSGAASSLGEGMKRGIEARFAEVNEAGGVHGESLTLVTRDDGYEPLRS
AKATRELLDDDEVLAMLGNVGTPTAIVNLPILLEHEMPLIGALTGSGVLRQSPPDRYVFNYRASYAQETATMIEGLLLAG
IAPEEIAFFTQADGYGDAGYRGALRSLANHGYSHPERLAHGRYTRGTRNVHRGVATILQAPVTPKAVIIVGTYGPAADFI
REARKDLPDALFLNVSFVGSQALLDELGDAAEGVIVTQVVPPHTSDLPGVEAYRAALQANGADSDGDFVSLEGYMAASLF
VEGLQAAGADPDREALVDGLEGLGKVDLGLGEPLTLGPEDHQANDAVWATRIHDGRFEPVDWFTLLDERAAEE

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994941, Length=315, Percent_Identity=23.1746031746032, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 41823; Mature: 41823

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVQRRWLAGALALALVAMPAWAEIKVGMVAPFSGAASSLGEGMKRGIEARFAEVNEAGG
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
VHGESLTLVTRDDGYEPLRSAKATRELLDDDEVLAMLGNVGTPTAIVNLPILLEHEMPLI
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCEEEECCCCEE
GALTGSGVLRQSPPDRYVFNYRASYAQETATMIEGLLLAGIAPEEIAFFTQADGYGDAGY
EEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCCHH
RGALRSLANHGYSHPERLAHGRYTRGTRNVHRGVATILQAPVTPKAVIIVGTYGPAADFI
HHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
REARKDLPDALFLNVSFVGSQALLDELGDAAEGVIVTQVVPPHTSDLPGVEAYRAALQAN
HHHHHCCCCEEEEEEEHHCHHHHHHHHCCHHCCEEEEEECCCCCCCCCCHHHHHHHHHCC
GADSDGDFVSLEGYMAASLFVEGLQAAGADPDREALVDGLEGLGKVDLGLGEPLTLGPED
CCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCC
HQANDAVWATRIHDGRFEPVDWFTLLDERAAEE
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVQRRWLAGALALALVAMPAWAEIKVGMVAPFSGAASSLGEGMKRGIEARFAEVNEAGG
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
VHGESLTLVTRDDGYEPLRSAKATRELLDDDEVLAMLGNVGTPTAIVNLPILLEHEMPLI
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCEEEECCCCEE
GALTGSGVLRQSPPDRYVFNYRASYAQETATMIEGLLLAGIAPEEIAFFTQADGYGDAGY
EEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCCHH
RGALRSLANHGYSHPERLAHGRYTRGTRNVHRGVATILQAPVTPKAVIIVGTYGPAADFI
HHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHH
REARKDLPDALFLNVSFVGSQALLDELGDAAEGVIVTQVVPPHTSDLPGVEAYRAALQAN
HHHHHCCCCEEEEEEEHHCHHHHHHHHCCHHCCEEEEEECCCCCCCCCCHHHHHHHHHCC
GADSDGDFVSLEGYMAASLFVEGLQAAGADPDREALVDGLEGLGKVDLGLGEPLTLGPED
CCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCC
HQANDAVWATRIHDGRFEPVDWFTLLDERAAEE
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA