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The map label for this gene is scoF [H]

Identifier: 92113792

GI number: 92113792

Start: 1898062

End: 1898265

Strand: Direct

Name: scoF [H]

Synonym: Csal_1668

Alternate gene names: 92113792

Gene position: 1898062-1898265 (Clockwise)

Preceding gene: 92113791

Following gene: 92113794

Centisome position: 51.35

GC content: 50.98

Gene sequence:

>204_bases
ATGGCTACTGGCACAGTTAAGTGGTTCAACGACGCTAAAGGTTTTGGCTTCATTTCTCCGGAAGACGGTGGCGACGATCT
CTTCGCTCACTTCTCCGAGATCCAGGCTGAAGGCTTCAAGTCCCTACAAGACGGTCAAAAGGTCTCTTTTGACGTGACTC
AGGGCAAAAAAGGCCTCCAGGCTTCCAACATCAAGCCTGCCTAA

Upstream 100 bases:

>100_bases
CGATACCCTCGATGGTCTGGTGTATAGCTTATTTGTCTTACCCACTGTAATCGGGTAACACCCGGCAATTGAAAGCGCAA
TGCGTAAAGGATATCGAAAC

Downstream 100 bases:

>100_bases
ATAGCGCGCTGTTCTCAGCCGGTATTAAGGGGCGCTAGCCTCGGCGCCACTTGGCTACGATGGCTAGCACCAAGAAACAG
GGCCCGCTTCGGCGGGCCCT

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MATGTVKWFNDAKGFGFISPEDGGDDLFAHFSEIQAEGFKSLQDGQKVSFDVTQGKKGLQASNIKPA

Sequences:

>Translated_67_residues
MATGTVKWFNDAKGFGFISPEDGGDDLFAHFSEIQAEGFKSLQDGQKVSFDVTQGKKGLQASNIKPA
>Mature_66_residues
ATGTVKWFNDAKGFGFISPEDGGDDLFAHFSEIQAEGFKSLQDGQKVSFDVTQGKKGLQASNIKPA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=62.6865671641791, Blast_Score=92, Evalue=6e-21,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=60.655737704918, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=61.2903225806452, Blast_Score=82, Evalue=8e-18,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=57.3770491803279, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI2367114, Length=62, Percent_Identity=53.2258064516129, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=56.4516129032258, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787834, Length=62, Percent_Identity=53.2258064516129, Blast_Score=74, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=45.9016393442623, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7196; Mature: 7065

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTVKWFNDAKGFGFISPEDGGDDLFAHFSEIQAEGFKSLQDGQKVSFDVTQGKKGLQ
CCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
ASNIKPA
CCCCCCC
>Mature Secondary Structure 
ATGTVKWFNDAKGFGFISPEDGGDDLFAHFSEIQAEGFKSLQDGQKVSFDVTQGKKGLQ
CCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
ASNIKPA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]