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The map label for this gene is amiC [H]

Identifier: 92112343

GI number: 92112343

Start: 231848

End: 233053

Strand: Direct

Name: amiC [H]

Synonym: Csal_0208

Alternate gene names: 92112343

Gene position: 231848-233053 (Clockwise)

Preceding gene: 92112342

Following gene: 92112344

Centisome position: 6.27

GC content: 62.35

Gene sequence:

>1206_bases
ATGACATCGGCAACGAAGCCTGAACGCCCCATGAGACGTTCAGGCTTTTTTTGTGGGCTGGCTATGAAACCTGAGATTGC
TGTCGGGTTGCTCTATTCGACGACCGGCACTTATCAACGCATCAGCAACAATGCACGTAGCGGCGCCTTGCAGGCGGTCG
CCGATATCAACGCCGACGCGACGTCTGGCGTGACGCTGGTGGCGCACGAGTACGATCCGCATGGTGAGCTCGAAGCCTAT
CATCGGGGCGTCGAGGACTTGTTGGCACGGGGCGTGCGTCACGTGGTGGGCACCACGACATCGGCGAGCCGCAAGGACAT
CGTGCCGGATGTCGAGCGTGACGAGGCCCTGTTGTGGTACGCCTGCCCCTACGAGGGATTCGAGTGCAGCGAGAACGTGC
TCTATTCCGGCGGATGCCCGAGCCAGAATCTGGTCCCATTGCTGGCCTATGCCCTGCGCGAGCGCGGCAAGCGCGGCGTG
CTGATCGGCTCCAACTATGTGTGGGGCTGGGAGAGCAACCGCATTGCCCGCGAACTGGTGGAAGCGTCCCATGGCCGTAT
CGTGGCGGAAAAGTACTTCCACTTCGGCGATTGCGACTTCGAGGCCATCGTTCCCCGGATTCTGGAAGGCGCTGCTGACT
TCGTGCTCAACAACCTGGTCGGCGAGTCCTCCTATGCCTTCCTGGCCCAACTCGAGGCCGCCTGCGAACGCCAAGGCACC
GGCATCACCGTGCTCAGCTGCAATTTCACCGAGTGCGAGCTGCCGGCGATTCCTCGTTTACGGCATGTACGCCTGTTGTC
TTGCGGTCCTTGGTTCGATCATCCCGGCAACGGCTTCAGTGCGCGCCAACGCGTCCGACATGGCGCGCAGATCTATTCGA
GTTGTTACACCAACGCCTACAACGCCGTTCAGTTGCTGGCGCGGGGCTGCGATGCGGTAGGTAGCGACGACCCCTCGAAG
GTACTGGCGTGGCTGCATGCGACCTCGCATTCCACCTTGATGGGCGAGTTGGCGTTCAACCCTCGCAATAATCATCTGGC
ACTGCCGTGCTGCATCGCCGAGGCGGGAGACGAAGGCTTTCGCATCGTGCATCGCGAAAGCGGCGTGCGGGACGCCGACC
CCTACCTGACCCGCACCGGTATCCCCCAGTTCCGCGACGTGGTCGGTGCCGCGCCCACGCCGCCCCACTTGAGGGTCGTG
AAATGA

Upstream 100 bases:

>100_bases
TGCCTTGTTGCTTCAACGTTTTATCGCGAGCGGCACGCTTTTTCCTTGTCTTTCGACACAAGCCTGGGCCAATGCTCCTG
TAAGCCTTGAATGAACTCCA

Downstream 100 bases:

>100_bases
ACGCGGAACCTTTGACTCCCCGGTTATTGCTGGTGGATTGCGAACCGCGCTCGCAGGCGATGCTCGACAAGAGTCTGGCA
CGATTGGGCTTGCACGGCTG

Product: putative regulator protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MTSATKPERPMRRSGFFCGLAMKPEIAVGLLYSTTGTYQRISNNARSGALQAVADINADATSGVTLVAHEYDPHGELEAY
HRGVEDLLARGVRHVVGTTTSASRKDIVPDVERDEALLWYACPYEGFECSENVLYSGGCPSQNLVPLLAYALRERGKRGV
LIGSNYVWGWESNRIARELVEASHGRIVAEKYFHFGDCDFEAIVPRILEGAADFVLNNLVGESSYAFLAQLEAACERQGT
GITVLSCNFTECELPAIPRLRHVRLLSCGPWFDHPGNGFSARQRVRHGAQIYSSCYTNAYNAVQLLARGCDAVGSDDPSK
VLAWLHATSHSTLMGELAFNPRNNHLALPCCIAEAGDEGFRIVHRESGVRDADPYLTRTGIPQFRDVVGAAPTPPHLRVV
K

Sequences:

>Translated_401_residues
MTSATKPERPMRRSGFFCGLAMKPEIAVGLLYSTTGTYQRISNNARSGALQAVADINADATSGVTLVAHEYDPHGELEAY
HRGVEDLLARGVRHVVGTTTSASRKDIVPDVERDEALLWYACPYEGFECSENVLYSGGCPSQNLVPLLAYALRERGKRGV
LIGSNYVWGWESNRIARELVEASHGRIVAEKYFHFGDCDFEAIVPRILEGAADFVLNNLVGESSYAFLAQLEAACERQGT
GITVLSCNFTECELPAIPRLRHVRLLSCGPWFDHPGNGFSARQRVRHGAQIYSSCYTNAYNAVQLLARGCDAVGSDDPSK
VLAWLHATSHSTLMGELAFNPRNNHLALPCCIAEAGDEGFRIVHRESGVRDADPYLTRTGIPQFRDVVGAAPTPPHLRVV
K
>Mature_400_residues
TSATKPERPMRRSGFFCGLAMKPEIAVGLLYSTTGTYQRISNNARSGALQAVADINADATSGVTLVAHEYDPHGELEAYH
RGVEDLLARGVRHVVGTTTSASRKDIVPDVERDEALLWYACPYEGFECSENVLYSGGCPSQNLVPLLAYALRERGKRGVL
IGSNYVWGWESNRIARELVEASHGRIVAEKYFHFGDCDFEAIVPRILEGAADFVLNNLVGESSYAFLAQLEAACERQGTG
ITVLSCNFTECELPAIPRLRHVRLLSCGPWFDHPGNGFSARQRVRHGAQIYSSCYTNAYNAVQLLARGCDAVGSDDPSKV
LAWLHATSHSTLMGELAFNPRNNHLALPCCIAEAGDEGFRIVHRESGVRDADPYLTRTGIPQFRDVVGAAPTPPHLRVVK

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 43910; Mature: 43779

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00485 A_DEAMINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATKPERPMRRSGFFCGLAMKPEIAVGLLYSTTGTYQRISNNARSGALQAVADINADA
CCCCCCCCCCHHHCCCEEEEECCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHCCCCC
TSGVTLVAHEYDPHGELEAYHRGVEDLLARGVRHVVGTTTSASRKDIVPDVERDEALLWY
CCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
ACPYEGFECSENVLYSGGCPSQNLVPLLAYALRERGKRGVLIGSNYVWGWESNRIARELV
ECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHH
EASHGRIVAEKYFHFGDCDFEAIVPRILEGAADFVLNNLVGESSYAFLAQLEAACERQGT
HHHCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GITVLSCNFTECELPAIPRLRHVRLLSCGPWFDHPGNGFSARQRVRHGAQIYSSCYTNAY
CEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NAVQLLARGCDAVGSDDPSKVLAWLHATSHSTLMGELAFNPRNNHLALPCCIAEAGDEGF
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEECCCCCCE
RIVHRESGVRDADPYLTRTGIPQFRDVVGAAPTPPHLRVVK
EEEECCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCEEEEC
>Mature Secondary Structure 
TSATKPERPMRRSGFFCGLAMKPEIAVGLLYSTTGTYQRISNNARSGALQAVADINADA
CCCCCCCCCHHHCCCEEEEECCCCEEEEEEEECCCHHHHHHCCCHHHHHHHHHHCCCCC
TSGVTLVAHEYDPHGELEAYHRGVEDLLARGVRHVVGTTTSASRKDIVPDVERDEALLWY
CCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
ACPYEGFECSENVLYSGGCPSQNLVPLLAYALRERGKRGVLIGSNYVWGWESNRIARELV
ECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCCCCHHHHHHH
EASHGRIVAEKYFHFGDCDFEAIVPRILEGAADFVLNNLVGESSYAFLAQLEAACERQGT
HHHCCEEEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GITVLSCNFTECELPAIPRLRHVRLLSCGPWFDHPGNGFSARQRVRHGAQIYSSCYTNAY
CEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NAVQLLARGCDAVGSDDPSKVLAWLHATSHSTLMGELAFNPRNNHLALPCCIAEAGDEGF
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEEEECCCCCCE
RIVHRESGVRDADPYLTRTGIPQFRDVVGAAPTPPHLRVVK
EEEECCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]