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The map label for this gene is ilvB [H]

Identifier: 92112278

GI number: 92112278

Start: 164363

End: 166003

Strand: Direct

Name: ilvB [H]

Synonym: Csal_0143

Alternate gene names: 92112278

Gene position: 164363-166003 (Clockwise)

Preceding gene: 92112277

Following gene: 92112279

Centisome position: 4.45

GC content: 66.79

Gene sequence:

>1641_bases
ATGAGCACACCCAACACTGTCGGCGACGCCATCGTCGAGACCCTGATACAACAGGGCGTCAGCGCTGTTTACGGCGTGAT
TTCCATCCATAACCTGCCCATCGCCGATGCCATCGGTCGCCATGAGGCGCTGCGTTTCGTCCCGGCGCGTGGGGAGGCCG
GCGCGGTCACCATGGCGGATGCCCATGGCCGTCAACGTGGGTTGGGAGTGGCCCTGACCAGCACGGGCGCGGGGGCCGGG
AATGCCGTGGGTGCCCTGCTGGAGGCGCTGAACGCCGGTGCGCCACTGCTGCATATCACGGGGCAGGTCGAGCGCGATTA
CCTGGGGCGCGAGTCGGGCTTCATCCACGAAACCCAGGACCAGATCGGCTTTCTCCGTGCATGCTCGAAGCGCGCCTACA
GCGCCCATACGGCCGAGCAGGTCGTGCCGGTTCTGCGGCGTGCCATGCGCGACGCGATGACCCCGCCGATGGGGCCCGTC
AGCGTCGAGATTCCCATCGATCTGCAGGGCGCCACCATCGAGAGCCAGACGCTCGGCTATGCGGTCGCGCGAGCCGCGGC
CCCCACCTTCGACGAGGGCGGTTTCGCGTCGCTCGTCGAGCGCCTGAAGCAGGCGCGTCGCCCCATGCTCTGGGTCGGAG
GCGGCGCCTTGCAAGCCGGCGATGCCGTACGTCGTCTGGCCGATGCGGGCCTTCCTGTGATCTCCAGCACCCACGGGCGC
GGCATCCTGGCCGATAGCCACCCCCGCAGTCTGCGGGCCTTTCACAACGCCGCCGCCGTCGAGGAGCTCCTCGCGCAGTC
CGACCTGCTGATCGTGGCCGGTTCCAAGCTGCGCAGCAATGAGACCAAGACCTTCACCTTGCCGCTGCCGCGCCCGCTGG
TGCAGATCGATATCGACCCGGCGGCCTGCCACCGCACCTACCTTGTCGACGAGTTCCTCGAGGGGGATTGCACCGAGGTG
CTGGAGGCACTGGCCGAGCATTTCGAGGCGACCCGGCTGGGCGACGACGACTACGACACCGAAGTGGCCAGGGCGGTCGC
GGCGGCCGAGCGTGCGCTGCGCCAGCAAATGGGGCCGTATGCCGAACTCTGCGATGCCTTGCGGCGCGCGCTCCCGGGCG
ATGGCATCCTGGTGCGCGATATCACCATGTCGGGCAGTACCTGGGGCAGTCGCCTGTTTCCCATCGAGACACCCAACACC
AACGTGCATTCACTGGCGGGCGCCATCGGCATGGGCTTGTCCATGGCCATCGGCAGTGCGGTGGCCAAGCCCACCTGCAA
GGTGGTGGGCCTGGTCGGTGACGGGGGCTTGATGCTGGGCGTCGGCGAACTGGCCACGATGGTCCAGGAAAACCTCGACA
TGACGTTGATCGTCATGAACGACGGTGGTTATGGCGTGATGCGAGGCATCCAGCGCAACCACTTCTCCGATCGCCAGTAT
TACAACGAGCTTTTGACGCCGTCGTTCACCAGGCTGGCGGACGCCATGGGCCTGCCGCATTGGCACCTGTCGAGCGCGGA
CGAGGCAGCGCGAGTGCTGCAGGATGCGGTCGCACACGCAGGACCAGCGCTTGTGGAAGTCGACATGGCGTCCTTCGGGG
AACTGGTGTTCGCCGGCCCGCCGCAAAAGAAATTGTACTGA

Upstream 100 bases:

>100_bases
CTGGGACTCGGTCAGACGCCCAATCCTTGGTGCGACTGAGTCAGCGAAACATGACGTCCGATAACGAGAGCGGGGCCCGG
TGTCCCGAGCGAAGGTACCT

Downstream 100 bases:

>100_bases
GGCTTTCATCGCGCCGGCCGTGCGGGATGACGGCCGGTCCTTTTTCTTCATCGCGATGCGAGGAGGTGCCGACGACCCTC
GAGTCCTGAATCCGAACGAC

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSTPNTVGDAIVETLIQQGVSAVYGVISIHNLPIADAIGRHEALRFVPARGEAGAVTMADAHGRQRGLGVALTSTGAGAG
NAVGALLEALNAGAPLLHITGQVERDYLGRESGFIHETQDQIGFLRACSKRAYSAHTAEQVVPVLRRAMRDAMTPPMGPV
SVEIPIDLQGATIESQTLGYAVARAAAPTFDEGGFASLVERLKQARRPMLWVGGGALQAGDAVRRLADAGLPVISSTHGR
GILADSHPRSLRAFHNAAAVEELLAQSDLLIVAGSKLRSNETKTFTLPLPRPLVQIDIDPAACHRTYLVDEFLEGDCTEV
LEALAEHFEATRLGDDDYDTEVARAVAAAERALRQQMGPYAELCDALRRALPGDGILVRDITMSGSTWGSRLFPIETPNT
NVHSLAGAIGMGLSMAIGSAVAKPTCKVVGLVGDGGLMLGVGELATMVQENLDMTLIVMNDGGYGVMRGIQRNHFSDRQY
YNELLTPSFTRLADAMGLPHWHLSSADEAARVLQDAVAHAGPALVEVDMASFGELVFAGPPQKKLY

Sequences:

>Translated_546_residues
MSTPNTVGDAIVETLIQQGVSAVYGVISIHNLPIADAIGRHEALRFVPARGEAGAVTMADAHGRQRGLGVALTSTGAGAG
NAVGALLEALNAGAPLLHITGQVERDYLGRESGFIHETQDQIGFLRACSKRAYSAHTAEQVVPVLRRAMRDAMTPPMGPV
SVEIPIDLQGATIESQTLGYAVARAAAPTFDEGGFASLVERLKQARRPMLWVGGGALQAGDAVRRLADAGLPVISSTHGR
GILADSHPRSLRAFHNAAAVEELLAQSDLLIVAGSKLRSNETKTFTLPLPRPLVQIDIDPAACHRTYLVDEFLEGDCTEV
LEALAEHFEATRLGDDDYDTEVARAVAAAERALRQQMGPYAELCDALRRALPGDGILVRDITMSGSTWGSRLFPIETPNT
NVHSLAGAIGMGLSMAIGSAVAKPTCKVVGLVGDGGLMLGVGELATMVQENLDMTLIVMNDGGYGVMRGIQRNHFSDRQY
YNELLTPSFTRLADAMGLPHWHLSSADEAARVLQDAVAHAGPALVEVDMASFGELVFAGPPQKKLY
>Mature_545_residues
STPNTVGDAIVETLIQQGVSAVYGVISIHNLPIADAIGRHEALRFVPARGEAGAVTMADAHGRQRGLGVALTSTGAGAGN
AVGALLEALNAGAPLLHITGQVERDYLGRESGFIHETQDQIGFLRACSKRAYSAHTAEQVVPVLRRAMRDAMTPPMGPVS
VEIPIDLQGATIESQTLGYAVARAAAPTFDEGGFASLVERLKQARRPMLWVGGGALQAGDAVRRLADAGLPVISSTHGRG
ILADSHPRSLRAFHNAAAVEELLAQSDLLIVAGSKLRSNETKTFTLPLPRPLVQIDIDPAACHRTYLVDEFLEGDCTEVL
EALAEHFEATRLGDDDYDTEVARAVAAAERALRQQMGPYAELCDALRRALPGDGILVRDITMSGSTWGSRLFPIETPNTN
VHSLAGAIGMGLSMAIGSAVAKPTCKVVGLVGDGGLMLGVGELATMVQENLDMTLIVMNDGGYGVMRGIQRNHFSDRQYY
NELLTPSFTRLADAMGLPHWHLSSADEAARVLQDAVAHAGPALVEVDMASFGELVFAGPPQKKLY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=518, Percent_Identity=24.3243243243243, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=22.5402504472272, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=28.9330922242315, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87081685, Length=550, Percent_Identity=27.4545454545455, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1786717, Length=486, Percent_Identity=26.3374485596708, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=25.3496503496504, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788716, Length=546, Percent_Identity=23.4432234432234, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17531299, Length=490, Percent_Identity=26.734693877551, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=490, Percent_Identity=26.734693877551, Blast_Score=99, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=24.7787610619469, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321524, Length=521, Percent_Identity=21.1132437619962, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922626, Length=492, Percent_Identity=25.2032520325203, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57855; Mature: 57723

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPNTVGDAIVETLIQQGVSAVYGVISIHNLPIADAIGRHEALRFVPARGEAGAVTMAD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHEEEECCCCCCEEEEEC
AHGRQRGLGVALTSTGAGAGNAVGALLEALNAGAPLLHITGQVERDYLGRESGFIHETQD
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCCCCHH
QIGFLRACSKRAYSAHTAEQVVPVLRRAMRDAMTPPMGPVSVEIPIDLQGATIESQTLGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEECHHHHHH
AVARAAAPTFDEGGFASLVERLKQARRPMLWVGGGALQAGDAVRRLADAGLPVISSTHGR
HHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCCC
GILADSHPRSLRAFHNAAAVEELLAQSDLLIVAGSKLRSNETKTFTLPLPRPLVQIDIDP
EEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCEEEEECCH
AACHRTYLVDEFLEGDCTEVLEALAEHFEATRLGDDDYDTEVARAVAAAERALRQQMGPY
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
AELCDALRRALPGDGILVRDITMSGSTWGSRLFPIETPNTNVHSLAGAIGMGLSMAIGSA
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VAKPTCKVVGLVGDGGLMLGVGELATMVQENLDMTLIVMNDGGYGVMRGIQRNHFSDRQY
HCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHH
YNELLTPSFTRLADAMGLPHWHLSSADEAARVLQDAVAHAGPALVEVDMASFGELVFAGP
HHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCEEECCC
PQKKLY
CCCCCC
>Mature Secondary Structure 
STPNTVGDAIVETLIQQGVSAVYGVISIHNLPIADAIGRHEALRFVPARGEAGAVTMAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHEEEECCCCCCEEEEEC
AHGRQRGLGVALTSTGAGAGNAVGALLEALNAGAPLLHITGQVERDYLGRESGFIHETQD
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCCCCCCCCHH
QIGFLRACSKRAYSAHTAEQVVPVLRRAMRDAMTPPMGPVSVEIPIDLQGATIESQTLGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCEECHHHHHH
AVARAAAPTFDEGGFASLVERLKQARRPMLWVGGGALQAGDAVRRLADAGLPVISSTHGR
HHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCCC
GILADSHPRSLRAFHNAAAVEELLAQSDLLIVAGSKLRSNETKTFTLPLPRPLVQIDIDP
EEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCEEEEECCH
AACHRTYLVDEFLEGDCTEVLEALAEHFEATRLGDDDYDTEVARAVAAAERALRQQMGPY
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
AELCDALRRALPGDGILVRDITMSGSTWGSRLFPIETPNTNVHSLAGAIGMGLSMAIGSA
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
VAKPTCKVVGLVGDGGLMLGVGELATMVQENLDMTLIVMNDGGYGVMRGIQRNHFSDRQY
HCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCHHHH
YNELLTPSFTRLADAMGLPHWHLSSADEAARVLQDAVAHAGPALVEVDMASFGELVFAGP
HHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCEEECCC
PQKKLY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]