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The map label for this gene is nitA [H]

Identifier: 91789232

GI number: 91789232

Start: 3569077

End: 3570018

Strand: Direct

Name: nitA [H]

Synonym: Bpro_3376

Alternate gene names: 91789232

Gene position: 3569077-3570018 (Clockwise)

Preceding gene: 91789230

Following gene: 91789233

Centisome position: 68.63

GC content: 65.71

Gene sequence:

>942_bases
ATGACGAAGATTGCCATCATTCAGCGCCCGCCGGTCTTGCTCGACCGTGGCGCCACCATCACAAGGGCAGTGCAATGGGT
CGCCGAAGCAGCAGCGGCAGGGGCGTCGCTGATCGTCTTTCCCGAGTCGTTCATTCCGGGTTACCCGTCCTGGATATGGC
GACTCGCGGCAGGAAGGGATGGCGCCGTCATGGGCCAATTGCACGCCCGCTTGCTCGCCAACGCGGTTGACCTCGGTGGC
GGCGACTTGGCCGAACTGTGCGAGGCCGCCCGCGCCCATAACGTGACGATCGTGTGCGGCATCAATGAGCGAGATCGGGA
GCGTGGCGGCGGGACGCTCTACAACACCGTCGTTACCATTGGTGCCGACGGCAGGGTGCAGAACCGGCACCGCAAGTTGA
TGCCAACCAACCCTGAACGCATGGTTCATGGCTTGGGCGACGCGTCCGGGTTGCGCGTGGTCGACACGCCTGCCGGCCGG
ATCGGCTGCCTCATCTGCTGGGAGAACTACATGCCGTTGGCGCGCTACGCGCTCTACGCCCAGGGCGTGGAGATCTACAT
CGCGCCCACCTACGACAGCGGGGACGCCTGGATCGGCACGATGCGCCATATCGCGCTCGAAGGTCGGTGCTGGGTGGTCG
GTAGCGGCACGGCGTTGCGTGCCAGTGACATTCCGCAGGACTTCCCGGCCCGCGCAGAGCTGTTCCCCGATCCCGAGGAA
TGGATCAACGACGGCGATTCGGTGGTTGTCGATCCCATGGGGAAGGTCGTCGCGGGCCCATTGCGCCGAGAGGCCGGCAT
CTTGTATGCAGAGATCGATACGGCGCGCGTCGCGCCGTCACGCCGAACGCTCGACGTCACCGGGCACTACGCGCGCCCGG
ACATCTTCGAGCTACAGGTGCGCCGCACGCCGGTGACACCCGTGCGCTACGTTGACGGGTAA

Upstream 100 bases:

>100_bases
CCGGAGCGTAAACGCTTCAGATTCTGGACTGGTCTGGGCGCCAAGTCCAGCGCAAACTGTCCATGTCGGGCAACGCCTGC
CCAAGAAGGAGAAAGACGCC

Downstream 100 bases:

>100_bases
GCGCCGCGCGCCCAGTGATTGCAGGACCAACCGACCCGTTCCGTCGCTCGAACACTTGCCAGGATGGCGGCCGGCAACCG
GCAGTAAGACCCAAAATTTC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTKIAIIQRPPVLLDRGATITRAVQWVAEAAAAGASLIVFPESFIPGYPSWIWRLAAGRDGAVMGQLHARLLANAVDLGG
GDLAELCEAARAHNVTIVCGINERDRERGGGTLYNTVVTIGADGRVQNRHRKLMPTNPERMVHGLGDASGLRVVDTPAGR
IGCLICWENYMPLARYALYAQGVEIYIAPTYDSGDAWIGTMRHIALEGRCWVVGSGTALRASDIPQDFPARAELFPDPEE
WINDGDSVVVDPMGKVVAGPLRREAGILYAEIDTARVAPSRRTLDVTGHYARPDIFELQVRRTPVTPVRYVDG

Sequences:

>Translated_313_residues
MTKIAIIQRPPVLLDRGATITRAVQWVAEAAAAGASLIVFPESFIPGYPSWIWRLAAGRDGAVMGQLHARLLANAVDLGG
GDLAELCEAARAHNVTIVCGINERDRERGGGTLYNTVVTIGADGRVQNRHRKLMPTNPERMVHGLGDASGLRVVDTPAGR
IGCLICWENYMPLARYALYAQGVEIYIAPTYDSGDAWIGTMRHIALEGRCWVVGSGTALRASDIPQDFPARAELFPDPEE
WINDGDSVVVDPMGKVVAGPLRREAGILYAEIDTARVAPSRRTLDVTGHYARPDIFELQVRRTPVTPVRYVDG
>Mature_312_residues
TKIAIIQRPPVLLDRGATITRAVQWVAEAAAAGASLIVFPESFIPGYPSWIWRLAAGRDGAVMGQLHARLLANAVDLGGG
DLAELCEAARAHNVTIVCGINERDRERGGGTLYNTVVTIGADGRVQNRHRKLMPTNPERMVHGLGDASGLRVVDTPAGRI
GCLICWENYMPLARYALYAQGVEIYIAPTYDSGDAWIGTMRHIALEGRCWVVGSGTALRASDIPQDFPARAELFPDPEEW
INDGDSVVVDPMGKVVAGPLRREAGILYAEIDTARVAPSRRTLDVTGHYARPDIFELQVRRTPVTPVRYVDG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=303, Percent_Identity=27.0627062706271, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17557111, Length=315, Percent_Identity=41.2698412698413, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322027, Length=186, Percent_Identity=41.3978494623656, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6322026, Length=110, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000132
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.5.7 [H]

Molecular weight: Translated: 34033; Mature: 33902

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIAIIQRPPVLLDRGATITRAVQWVAEAAAAGASLIVFPESFIPGYPSWIWRLAAGRD
CCEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCEEEEECHHHCCCCHHHHHEECCCCC
GAVMGQLHARLLANAVDLGGGDLAELCEAARAHNVTIVCGINERDRERGGGTLYNTVVTI
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCEEEEEEEEE
GADGRVQNRHRKLMPTNPERMVHGLGDASGLRVVDTPAGRIGCLICWENYMPLARYALYA
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHH
QGVEIYIAPTYDSGDAWIGTMRHIALEGRCWVVGSGTALRASDIPQDFPARAELFPDPEE
CCEEEEEEECCCCCCCHHHHEEEEEECCEEEEEECCCEEECCCCCCCCCCCCCCCCCHHH
WINDGDSVVVDPMGKVVAGPLRREAGILYAEIDTARVAPSRRTLDVTGHYARPDIFELQV
HHCCCCEEEECCCCHHHHCCHHHHCCEEEEECCHHCCCCCCEEEEECCCCCCCCEEEEEE
RRTPVTPVRYVDG
ECCCCCCEEECCC
>Mature Secondary Structure 
TKIAIIQRPPVLLDRGATITRAVQWVAEAAAAGASLIVFPESFIPGYPSWIWRLAAGRD
CEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCEEEEECHHHCCCCHHHHHEECCCCC
GAVMGQLHARLLANAVDLGGGDLAELCEAARAHNVTIVCGINERDRERGGGTLYNTVVTI
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCEEEEEEEEE
GADGRVQNRHRKLMPTNPERMVHGLGDASGLRVVDTPAGRIGCLICWENYMPLARYALYA
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHH
QGVEIYIAPTYDSGDAWIGTMRHIALEGRCWVVGSGTALRASDIPQDFPARAELFPDPEE
CCEEEEEEECCCCCCCHHHHEEEEEECCEEEEEECCCEEECCCCCCCCCCCCCCCCCHHH
WINDGDSVVVDPMGKVVAGPLRREAGILYAEIDTARVAPSRRTLDVTGHYARPDIFELQV
HHCCCCEEEECCCCHHHHCCHHHHCCEEEEECCHHCCCCCCEEEEECCCCCCCCEEEEEE
RRTPVTPVRYVDG
ECCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400390 [H]