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The map label for this gene is llaDCHIA [H]

Identifier: 91789170

GI number: 91789170

Start: 3509240

End: 3510055

Strand: Direct

Name: llaDCHIA [H]

Synonym: Bpro_3313

Alternate gene names: 91789170

Gene position: 3509240-3510055 (Clockwise)

Preceding gene: 91789169

Following gene: 91789171

Centisome position: 67.48

GC content: 47.18

Gene sequence:

>816_bases
TTGCAGCCTGCAGCAACTACCGTAACTCCGTTTCTAAAGTGGGCTGGGGGGAAAAGGTGGCTTATCGAAAAGCATCCCTA
TCTACTTGGTGGAAAGTTCACCAGATTCATTGAGCCCTTTGTTGGCGGGGGCGCGGTTTTCTTTAGCCTACAACCCGACT
CTGCCATCCTATGTGACAAAAATGAAAAGTTGATTGAGGTTTATGCGACCATCAAATCAAACTGGCAACGCGTGGAAGAT
CTTCTCGTGGAGCATCAGAACAAGCACTCGCCTGAATATTATTACGAGATAAGGTCCAAAGACTATAGGACGCCTGAGAC
GCGTACGGCTCAACTAATTTATCTCAATAGGACTTGTTGGAATGGGTTGTACCGTGTCAATAAGAAAGGCGAATTCAATG
TTCCGGTCGGAACAAAGCAGACCGTAATTCTTCAAACCGATAACTTTGAAGGTGTGGCATCAATTCTGAAACGGGCCGAG
TTGATTTGTGGTGACTTCGACGTTGCTATGAGCAAAGCTGGGGAAAACGATTTTGTATTTGTTGACCCCCCATACACAGT
GAAGCACAACTACAACGGCTTCGTAAAGTACAACGAAAATATATTCTCTTGGGACGACCAACTACGGCTGCGGGATGCTG
TGAAAGCGGCCATTTCGCGCGGTGCAAAGGTTTTGGTCACGAACGCTTGCCATGAAAGCATTAAGCGGATCTATGCTGGC
ATTGGAGAAATGTGTACCGTTGATAGAGCAAGCGTAATTGCCGGAAAAGCGTCTGCGCGCGGCAGGTACGAGGAGGTGAT
CATCAAATGTTTCTGA

Upstream 100 bases:

>100_bases
TGCTGATACCATCGCCAGTATCTTTCAATCGCCCGCCATACCCAGCATTGGTGCCATGCGAATGGTGTGGTGGAGTTAAA
AAATTGTCCGGAGTATTCCC

Downstream 100 bases:

>100_bases
AAGTTCTTGGTCGTATTCCACTTCTGCAGTGCTTGACTTACTTTCCGGCCCAGGCTCATAAGTCGGCAAGCAAGAAATTT
TTCGCTCTACTATTTTTAAC

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MQPAATTVTPFLKWAGGKRWLIEKHPYLLGGKFTRFIEPFVGGGAVFFSLQPDSAILCDKNEKLIEVYATIKSNWQRVED
LLVEHQNKHSPEYYYEIRSKDYRTPETRTAQLIYLNRTCWNGLYRVNKKGEFNVPVGTKQTVILQTDNFEGVASILKRAE
LICGDFDVAMSKAGENDFVFVDPPYTVKHNYNGFVKYNENIFSWDDQLRLRDAVKAAISRGAKVLVTNACHESIKRIYAG
IGEMCTVDRASVIAGKASARGRYEEVIIKCF

Sequences:

>Translated_271_residues
MQPAATTVTPFLKWAGGKRWLIEKHPYLLGGKFTRFIEPFVGGGAVFFSLQPDSAILCDKNEKLIEVYATIKSNWQRVED
LLVEHQNKHSPEYYYEIRSKDYRTPETRTAQLIYLNRTCWNGLYRVNKKGEFNVPVGTKQTVILQTDNFEGVASILKRAE
LICGDFDVAMSKAGENDFVFVDPPYTVKHNYNGFVKYNENIFSWDDQLRLRDAVKAAISRGAKVLVTNACHESIKRIYAG
IGEMCTVDRASVIAGKASARGRYEEVIIKCF
>Mature_271_residues
MQPAATTVTPFLKWAGGKRWLIEKHPYLLGGKFTRFIEPFVGGGAVFFSLQPDSAILCDKNEKLIEVYATIKSNWQRVED
LLVEHQNKHSPEYYYEIRSKDYRTPETRTAQLIYLNRTCWNGLYRVNKKGEFNVPVGTKQTVILQTDNFEGVASILKRAE
LICGDFDVAMSKAGENDFVFVDPPYTVKHNYNGFVKYNENIFSWDDQLRLRDAVKAAISRGAKVLVTNACHESIKRIYAG
IGEMCTVDRASVIAGKASARGRYEEVIIKCF

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=265, Percent_Identity=29.811320754717, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 30827; Mature: 30827

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPAATTVTPFLKWAGGKRWLIEKHPYLLGGKFTRFIEPFVGGGAVFFSLQPDSAILCDK
CCCCCCHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHCCCCEEEEEECCCCEEEECC
NEKLIEVYATIKSNWQRVEDLLVEHQNKHSPEYYYEIRSKDYRTPETRTAQLIYLNRTCW
CCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEECHHH
NGLYRVNKKGEFNVPVGTKQTVILQTDNFEGVASILKRAELICGDFDVAMSKAGENDFVF
CCEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEE
VDPPYTVKHNYNGFVKYNENIFSWDDQLRLRDAVKAAISRGAKVLVTNACHESIKRIYAG
ECCCEEEEECCCEEEEECCCEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHC
IGEMCTVDRASVIAGKASARGRYEEVIIKCF
CCCHHCCCCHHHEECCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MQPAATTVTPFLKWAGGKRWLIEKHPYLLGGKFTRFIEPFVGGGAVFFSLQPDSAILCDK
CCCCCCHHHHHHHHCCCCEEEEECCCEEECCHHHHHHHHHCCCCEEEEEECCCCEEEECC
NEKLIEVYATIKSNWQRVEDLLVEHQNKHSPEYYYEIRSKDYRTPETRTAQLIYLNRTCW
CCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEECHHH
NGLYRVNKKGEFNVPVGTKQTVILQTDNFEGVASILKRAELICGDFDVAMSKAGENDFVF
CCEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEE
VDPPYTVKHNYNGFVKYNENIFSWDDQLRLRDAVKAAISRGAKVLVTNACHESIKRIYAG
ECCCEEEEECCCEEEEECCCEECCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHC
IGEMCTVDRASVIAGKASARGRYEEVIIKCF
CCCHHCCCCHHHEECCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]