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The map label for this gene is ubiE [H]

Identifier: 91789165

GI number: 91789165

Start: 3504359

End: 3505129

Strand: Direct

Name: ubiE [H]

Synonym: Bpro_3308

Alternate gene names: 91789165

Gene position: 3504359-3505129 (Clockwise)

Preceding gene: 91789164

Following gene: 91789169

Centisome position: 67.39

GC content: 58.24

Gene sequence:

>771_bases
ATGAAGACTTCCGAACCCGTCCCTACGACGCATGCACCCCATATTCCGCTCACTGATTACTACCAGACAGAGCAGGATAG
ACAAGCCTATTTGCGCAAGATTTTTGACAATACCGCTGCGGATTACGACCGTATCGAGGCGATGTTGGCATGGGGCACTG
GTTCCCGTTACCGGCACCAGGCCCTGATCCGGGGCGGTTTGCAAGCGGGCATGAAAGTGCTCGATGTGGGTGTGGGAACC
GGCCTGGTTGCGGCGCAGGCCTGCCTCCTGGCCGGCGATCCCGCCTTGGTCACCGGGGTTGATCCCAGCCCCGGCATGAT
GGCGGCCAGCAAGCTGCCCAAGACCATGGTGCTGATGGAGGGCCGGGCCGAATCATTGCCTTTTCCGGACAACCACTTTG
ATTTCCTGAGCATGGGCTATGCACTTCGCCACATCAGTGACCTGGGTGTGGCGTTTGCAGAGTTTCAACGGGTATTGAAG
CCCGGTGGCCGTCTGTGCATCCTTGAAATAACCCAGGCGAAAAGCGGCTTGGGGCAGTGGCTGCTCAAAACTTACATGCG
GGGTGTGATACCGCTGCTGACGCGGTTTATTTCCCGGGAAAAAGATACAGCAACGATCTGGCGTTACTACTGGGACAGCA
TAGAAGCCTGCGTCCCTCCCGAACAAGTCATGGCCACACTGAGGGCGGCCGGGCTGACGCAGGTCAAGCAACACCTGGAG
GTTGGCGTGTTTTCGGAATACCAGGCGATCAAACCCGGGAACGCCAGTTGA

Upstream 100 bases:

>100_bases
GCATCAGCCTGCGTCATCGTCGCACCTTGCCAACGCACGCCCATGACGGCCCACACCCGGCGCGTCCAACTGCCGTTTCA
GGTCTACAGGTAAATGCCCC

Downstream 100 bases:

>100_bases
CGGCCTGCCGCAGGAAGGCAGGCATCATGAGCACTGGCTTTGGGACGAACACCGTCACCAGGAGGGGAAGCGCCAGCCCA
GCCTGAACCGTCAGTACTCA

Product: demethylmenaquinone methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKTSEPVPTTHAPHIPLTDYYQTEQDRQAYLRKIFDNTAADYDRIEAMLAWGTGSRYRHQALIRGGLQAGMKVLDVGVGT
GLVAAQACLLAGDPALVTGVDPSPGMMAASKLPKTMVLMEGRAESLPFPDNHFDFLSMGYALRHISDLGVAFAEFQRVLK
PGGRLCILEITQAKSGLGQWLLKTYMRGVIPLLTRFISREKDTATIWRYYWDSIEACVPPEQVMATLRAAGLTQVKQHLE
VGVFSEYQAIKPGNAS

Sequences:

>Translated_256_residues
MKTSEPVPTTHAPHIPLTDYYQTEQDRQAYLRKIFDNTAADYDRIEAMLAWGTGSRYRHQALIRGGLQAGMKVLDVGVGT
GLVAAQACLLAGDPALVTGVDPSPGMMAASKLPKTMVLMEGRAESLPFPDNHFDFLSMGYALRHISDLGVAFAEFQRVLK
PGGRLCILEITQAKSGLGQWLLKTYMRGVIPLLTRFISREKDTATIWRYYWDSIEACVPPEQVMATLRAAGLTQVKQHLE
VGVFSEYQAIKPGNAS
>Mature_256_residues
MKTSEPVPTTHAPHIPLTDYYQTEQDRQAYLRKIFDNTAADYDRIEAMLAWGTGSRYRHQALIRGGLQAGMKVLDVGVGT
GLVAAQACLLAGDPALVTGVDPSPGMMAASKLPKTMVLMEGRAESLPFPDNHFDFLSMGYALRHISDLGVAFAEFQRVLK
PGGRLCILEITQAKSGLGQWLLKTYMRGVIPLLTRFISREKDTATIWRYYWDSIEACVPPEQVMATLRAAGLTQVKQHLE
VGVFSEYQAIKPGNAS

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=226, Percent_Identity=29.646017699115, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557039, Length=264, Percent_Identity=28.030303030303, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28217; Mature: 28217

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSEPVPTTHAPHIPLTDYYQTEQDRQAYLRKIFDNTAADYDRIEAMLAWGTGSRYRHQ
CCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
ALIRGGLQAGMKVLDVGVGTGLVAAQACLLAGDPALVTGVDPSPGMMAASKLPKTMVLME
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHCCCEEEEEC
GRAESLPFPDNHFDFLSMGYALRHISDLGVAFAEFQRVLKPGGRLCILEITQAKSGLGQW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHH
LLKTYMRGVIPLLTRFISREKDTATIWRYYWDSIEACVPPEQVMATLRAAGLTQVKQHLE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VGVFSEYQAIKPGNAS
CCCHHCHHCCCCCCCC
>Mature Secondary Structure
MKTSEPVPTTHAPHIPLTDYYQTEQDRQAYLRKIFDNTAADYDRIEAMLAWGTGSRYRHQ
CCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
ALIRGGLQAGMKVLDVGVGTGLVAAQACLLAGDPALVTGVDPSPGMMAASKLPKTMVLME
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHCCCEEEEEC
GRAESLPFPDNHFDFLSMGYALRHISDLGVAFAEFQRVLKPGGRLCILEITQAKSGLGQW
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHH
LLKTYMRGVIPLLTRFISREKDTATIWRYYWDSIEACVPPEQVMATLRAAGLTQVKQHLE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VGVFSEYQAIKPGNAS
CCCHHCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA