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The map label for this gene is gatA [H]

Identifier: 91789150

GI number: 91789150

Start: 3485745

End: 3487040

Strand: Direct

Name: gatA [H]

Synonym: Bpro_3290

Alternate gene names: 91789150

Gene position: 3485745-3487040 (Clockwise)

Preceding gene: 91789149

Following gene: 91789153

Centisome position: 67.03

GC content: 69.21

Gene sequence:

>1296_bases
ATGACGCCCGCCGCGCCCTCGGCCATCGCCACCGCCGCGCAATTGGCGGCGGGCAAGCTTGACGCCACCACGCTGCTGGC
CGACACCCTGGCGCGCATTGATGCGCAGGATGCTGCGCTGGGCGCCTTTGTCGCGCTGCTGAACCCCGGCGCCGCGCGCG
AGCAGCTGGGGCGACTCCAGGAAGCCGGCGGCCCGCTGCAGGGCCTGCCGGTGGCCGTCAAGGACATCTTTGACACCGCA
GGCCTGCCCACCAGTTACGGCTCGGCACTTTACGCTGGCGCCCAACCGCGTTGTGACGCGGCCATCGTCAGCGTGATCCG
CCGCGCTGGCGGGCTGGTGATCGGAAAATCAAGCACCACGGAATTTGCCTTTCTGCATCCCACCGCCACGCGCAACCCGA
ATGCCCCGGGCCGAACGCCGGGCGGTTCCTCCGCCGGGTCGGCGGCTGCAGTCGCGGCGGGTCTTGTGCCTTTTGCGGTT
GGCACCCAAACCGGCGGCTCCGTCATTCGCCCCGCGTCCTATTGCGGCGTCACCGGCTTCAAGCCCAGCTTTGGCCTGTT
GCCGACGGCCGGCCTCAAATGCTTTTCATGGTCGCTCGACACCGTGGGCCTGTTTGCCCGCAACGTGGCCGACGTGGCGT
GGTTTGCGCAGGCGGTGAGCGGACACGCGCTGGCGCTGCCGGCCGCGCAGGCGACACCCGCAAAGTCCTGGGTGGTGGGC
GTGCCCGACTCTTACCCCTGGGGGCCGGTATCGCCCAGCGCCCAGCGAGCGATTGAAGCCGGCAGCCAGGCCCTGCGGGC
CGCCGGCGCCAAAGTGCTGGGCATCACCCTGCCGGCCTGGATGAAGGACGTCCATGAAGCGCAGGATGTGATCCAGGGCT
ACGAAGCCTGGCGTGCGCTGGCGCGCGAGGTCGACCAGCTGGCCCGGCAACCCGAGGCGCTGTCGACCATTCTGCGCGAC
TACCTCATCACCGCAAGCCGGATCAGCGCGCCAGCTTACGAAGCCGCGCAGCAGACCGCGCGGCAGGGCCGACAGGCCTG
CGCAGACTGGTTTGGCCAGTTTGATGTGCTGATGACGCCCAGCGCCCCCGATGAAGCACCGCCGGGCTATGGCAGCACCG
GCAGTTCCATGTTCAACCGCGCCTGGACGCTGCTGGGTTTGCCGTGTGTGAATGTGGCGGGCGCCGTGGGCGTGAACGGC
TTCCCGATGGGCCTGCAGGTCATTGGCGCTCCCCACGCAGACCGTGCCTGCCTTGAAGCCGCCGACGTTCTGGAACGATC
GCTGCGCGCGACTTGA

Upstream 100 bases:

>100_bases
TGATCGGCGGCCCCTACCTCGAAACCGATCCGGCGGTCTGCAAGGCCTTCCGGCCCAAGTCGGCAATTCGTACTCCGGGT
ACCCCGGGCACACCGGCCCC

Downstream 100 bases:

>100_bases
ACGCGATCTGAAAAACCGGTTGTCCTGGGCTTATTTTCCGACCAGTTGGTTCAGCTTGTCCGCTTCAAAGGTCTCGTGCA
GCGCGGCATCCTTGGGTACG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTPAAPSAIATAAQLAAGKLDATTLLADTLARIDAQDAALGAFVALLNPGAAREQLGRLQEAGGPLQGLPVAVKDIFDTA
GLPTSYGSALYAGAQPRCDAAIVSVIRRAGGLVIGKSSTTEFAFLHPTATRNPNAPGRTPGGSSAGSAAAVAAGLVPFAV
GTQTGGSVIRPASYCGVTGFKPSFGLLPTAGLKCFSWSLDTVGLFARNVADVAWFAQAVSGHALALPAAQATPAKSWVVG
VPDSYPWGPVSPSAQRAIEAGSQALRAAGAKVLGITLPAWMKDVHEAQDVIQGYEAWRALAREVDQLARQPEALSTILRD
YLITASRISAPAYEAAQQTARQGRQACADWFGQFDVLMTPSAPDEAPPGYGSTGSSMFNRAWTLLGLPCVNVAGAVGVNG
FPMGLQVIGAPHADRACLEAADVLERSLRAT

Sequences:

>Translated_431_residues
MTPAAPSAIATAAQLAAGKLDATTLLADTLARIDAQDAALGAFVALLNPGAAREQLGRLQEAGGPLQGLPVAVKDIFDTA
GLPTSYGSALYAGAQPRCDAAIVSVIRRAGGLVIGKSSTTEFAFLHPTATRNPNAPGRTPGGSSAGSAAAVAAGLVPFAV
GTQTGGSVIRPASYCGVTGFKPSFGLLPTAGLKCFSWSLDTVGLFARNVADVAWFAQAVSGHALALPAAQATPAKSWVVG
VPDSYPWGPVSPSAQRAIEAGSQALRAAGAKVLGITLPAWMKDVHEAQDVIQGYEAWRALAREVDQLARQPEALSTILRD
YLITASRISAPAYEAAQQTARQGRQACADWFGQFDVLMTPSAPDEAPPGYGSTGSSMFNRAWTLLGLPCVNVAGAVGVNG
FPMGLQVIGAPHADRACLEAADVLERSLRAT
>Mature_430_residues
TPAAPSAIATAAQLAAGKLDATTLLADTLARIDAQDAALGAFVALLNPGAAREQLGRLQEAGGPLQGLPVAVKDIFDTAG
LPTSYGSALYAGAQPRCDAAIVSVIRRAGGLVIGKSSTTEFAFLHPTATRNPNAPGRTPGGSSAGSAAAVAAGLVPFAVG
TQTGGSVIRPASYCGVTGFKPSFGLLPTAGLKCFSWSLDTVGLFARNVADVAWFAQAVSGHALALPAAQATPAKSWVVGV
PDSYPWGPVSPSAQRAIEAGSQALRAAGAKVLGITLPAWMKDVHEAQDVIQGYEAWRALAREVDQLARQPEALSTILRDY
LITASRISAPAYEAAQQTARQGRQACADWFGQFDVLMTPSAPDEAPPGYGSTGSSMFNRAWTLLGLPCVNVAGAVGVNGF
PMGLQVIGAPHADRACLEAADVLERSLRAT

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=25.1012145748988, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI195972892, Length=416, Percent_Identity=23.5576923076923, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=428, Percent_Identity=28.0373831775701, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=292, Percent_Identity=26.027397260274, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=28.1725888324873, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=224, Percent_Identity=29.9107142857143, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648113, Length=453, Percent_Identity=26.2693156732892, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078093, Length=477, Percent_Identity=25.9958071278826, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356731, Length=471, Percent_Identity=24.416135881104, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648441, Length=399, Percent_Identity=24.5614035087719, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648439, Length=399, Percent_Identity=24.5614035087719, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648435, Length=403, Percent_Identity=25.3101736972705, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648437, Length=403, Percent_Identity=25.3101736972705, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550774, Length=403, Percent_Identity=25.3101736972705, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24644968, Length=482, Percent_Identity=24.0663900414938, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 44112; Mature: 43981

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAAPSAIATAAQLAAGKLDATTLLADTLARIDAQDAALGAFVALLNPGAAREQLGRLQ
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHH
EAGGPLQGLPVAVKDIFDTAGLPTSYGSALYAGAQPRCDAAIVSVIRRAGGLVIGKSSTT
HCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCEEEECCCCC
EFAFLHPTATRNPNAPGRTPGGSSAGSAAAVAAGLVPFAVGTQTGGSVIRPASYCGVTGF
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
KPSFGLLPTAGLKCFSWSLDTVGLFARNVADVAWFAQAVSGHALALPAAQATPAKSWVVG
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEE
VPDSYPWGPVSPSAQRAIEAGSQALRAAGAKVLGITLPAWMKDVHEAQDVIQGYEAWRAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
AREVDQLARQPEALSTILRDYLITASRISAPAYEAAQQTARQGRQACADWFGQFDVLMTP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
SAPDEAPPGYGSTGSSMFNRAWTLLGLPCVNVAGAVGVNGFPMGLQVIGAPHADRACLEA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHH
ADVLERSLRAT
HHHHHHHHCCC
>Mature Secondary Structure 
TPAAPSAIATAAQLAAGKLDATTLLADTLARIDAQDAALGAFVALLNPGAAREQLGRLQ
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHH
EAGGPLQGLPVAVKDIFDTAGLPTSYGSALYAGAQPRCDAAIVSVIRRAGGLVIGKSSTT
HCCCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCEEEECCCCC
EFAFLHPTATRNPNAPGRTPGGSSAGSAAAVAAGLVPFAVGTQTGGSVIRPASYCGVTGF
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
KPSFGLLPTAGLKCFSWSLDTVGLFARNVADVAWFAQAVSGHALALPAAQATPAKSWVVG
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEE
VPDSYPWGPVSPSAQRAIEAGSQALRAAGAKVLGITLPAWMKDVHEAQDVIQGYEAWRAL
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
AREVDQLARQPEALSTILRDYLITASRISAPAYEAAQQTARQGRQACADWFGQFDVLMTP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECC
SAPDEAPPGYGSTGSSMFNRAWTLLGLPCVNVAGAVGVNGFPMGLQVIGAPHADRACLEA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHH
ADVLERSLRAT
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA