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The map label for this gene is kefA [H]

Identifier: 91789004

GI number: 91789004

Start: 3319270

End: 3320604

Strand: Reverse

Name: kefA [H]

Synonym: Bpro_3144

Alternate gene names: 91789004

Gene position: 3320604-3319270 (Counterclockwise)

Preceding gene: 91789005

Following gene: 91789003

Centisome position: 63.85

GC content: 63.6

Gene sequence:

>1335_bases
ATGGCCAGCGCCGGCGCCCTGATTCCCGCCAATAGCTCACTCGTCCGTCCCCCAGCCCGCATCGATAACCTGGATGCCTG
GTTTGCAGCACTGACCCAGCCCTCCGCGCTGACTGAACTGGCCTCGCTGGTTGCATGCGTCCTGCTGGCCTGGCTGGTGG
TACGTGTGGCCAGCCGTGCCCGGCACAGTGCTGACGAGTCATCCATCCTCTTTGGCCGGCGCATTGTGGACGGGGTGCTG
TTCCCGCTGCTGCTTTTGTCTTTTGCCTATGCGGCCCAGGCGCTGCTGGCGCGGATGTTTCCGCTGGCAGTCTTCAAGGT
GGTCATTCCAGTGCTGCTGGCGCTGGCGCTGATTCGCCTGGGCGTCAAGGTGCTGCAGGTGGCGTTTAAAAATGCGCCGC
TGGTGCGGGCGCTGGAGCGCACGATTTCATGGCTGGCCTGGATTGCCATGGTGCTCTGGGTCAGCGGCCTGCTGCCGGTC
ATTCTTGAGGAGCTGGATGGCATCAAGTGGAAGGTCGGCAACTCGACCCTGTCGGTGCGCACCATGCTTGAAGGGGCGCT
GACGGCCGGCGTGGTGCTGTTGATCGCGCTGTGGATTTCCTCGGCCCTGGAGGCGCGCCTGCTGCGCTCCGCCACAGGTG
GCGAACTGTCCTTGCGCAAGGCGGTGAGCAATGCCGCCCGGGTGGTGCTGATGTTTGTGGGCCTGTTGCTGGCGCTGTCG
TCGGTGGGCATTGACCTGACGGCACTGTCGGTGCTGGGCGGCGCGATCGGCGTGGGTGTGGGCTTTGGCCTGCAAAAACT
GGCCGCCAGCTATGTGAGCGGTTTCGTGATGCTGGCCGAGCGCAGCGTGCGTATCGGGGACAACGTGCGTGTCGATACCT
TCGAAGGCCGCATCACCGACATCAACGCCCGCTACACCGTCGTGCGGGCACCATCCGGCCGCGAATCCATCGTGCCCAAC
GAGATGTTCATCAGCAACCGCATCGAAAATCTTTCGCTCAGTGATTTGCGCGTGCTGCAGTCCACCGTGGTGTCGGTGGG
CTATGACAGCGATGTCGCGCTGGTGATGCGTTTGCTGGCCGAAGCGGCGCTCAGTCAGCCGCGCGTGCTGCGCGATCCCG
AGCCTTCCGTGAACCTGACCAATTTCGGGGCCGATGGGCTGGAGTTCACGCTCAACTACTGGATGACCGACCCCGAAAAC
GGCCAGCAAAACCTGCGCTCCAGCATCAACCTGGAGGTGCTGAAGGCCCTGCGTGAGAACGGTATCGAGATTCCGTATCC
GCAGCGGGTGATCCATACGCGTGTCAGCGGTGTGGAGGGCGCCAGGACTTCCTGA

Upstream 100 bases:

>100_bases
ACCGCCGGGCGTCAGGCCGTGTGAACAGTTTCTCGCCACAGCGGTGAAATGTCTCTTTATTGAACAAAAATTCGAACACA
GTTGAACAAAGGAAACCGCC

Downstream 100 bases:

>100_bases
GTTGCCGCAGCGCAGGGCGTCGGCGTCCCCGGGGCGATTCCCCAATTGACGCCTGCACGACAGCTGTTTGGTAGGGATGC
TCTAATTCCTCTGGGGGAGC

Product: mechanosensitive ion channel protein MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MASAGALIPANSSLVRPPARIDNLDAWFAALTQPSALTELASLVACVLLAWLVVRVASRARHSADESSILFGRRIVDGVL
FPLLLLSFAYAAQALLARMFPLAVFKVVIPVLLALALIRLGVKVLQVAFKNAPLVRALERTISWLAWIAMVLWVSGLLPV
ILEELDGIKWKVGNSTLSVRTMLEGALTAGVVLLIALWISSALEARLLRSATGGELSLRKAVSNAARVVLMFVGLLLALS
SVGIDLTALSVLGGAIGVGVGFGLQKLAASYVSGFVMLAERSVRIGDNVRVDTFEGRITDINARYTVVRAPSGRESIVPN
EMFISNRIENLSLSDLRVLQSTVVSVGYDSDVALVMRLLAEAALSQPRVLRDPEPSVNLTNFGADGLEFTLNYWMTDPEN
GQQNLRSSINLEVLKALRENGIEIPYPQRVIHTRVSGVEGARTS

Sequences:

>Translated_444_residues
MASAGALIPANSSLVRPPARIDNLDAWFAALTQPSALTELASLVACVLLAWLVVRVASRARHSADESSILFGRRIVDGVL
FPLLLLSFAYAAQALLARMFPLAVFKVVIPVLLALALIRLGVKVLQVAFKNAPLVRALERTISWLAWIAMVLWVSGLLPV
ILEELDGIKWKVGNSTLSVRTMLEGALTAGVVLLIALWISSALEARLLRSATGGELSLRKAVSNAARVVLMFVGLLLALS
SVGIDLTALSVLGGAIGVGVGFGLQKLAASYVSGFVMLAERSVRIGDNVRVDTFEGRITDINARYTVVRAPSGRESIVPN
EMFISNRIENLSLSDLRVLQSTVVSVGYDSDVALVMRLLAEAALSQPRVLRDPEPSVNLTNFGADGLEFTLNYWMTDPEN
GQQNLRSSINLEVLKALRENGIEIPYPQRVIHTRVSGVEGARTS
>Mature_443_residues
ASAGALIPANSSLVRPPARIDNLDAWFAALTQPSALTELASLVACVLLAWLVVRVASRARHSADESSILFGRRIVDGVLF
PLLLLSFAYAAQALLARMFPLAVFKVVIPVLLALALIRLGVKVLQVAFKNAPLVRALERTISWLAWIAMVLWVSGLLPVI
LEELDGIKWKVGNSTLSVRTMLEGALTAGVVLLIALWISSALEARLLRSATGGELSLRKAVSNAARVVLMFVGLLLALSS
VGIDLTALSVLGGAIGVGVGFGLQKLAASYVSGFVMLAERSVRIGDNVRVDTFEGRITDINARYTVVRAPSGRESIVPNE
MFISNRIENLSLSDLRVLQSTVVSVGYDSDVALVMRLLAEAALSQPRVLRDPEPSVNLTNFGADGLEFTLNYWMTDPENG
QQNLRSSINLEVLKALRENGIEIPYPQRVIHTRVSGVEGARTS

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=221, Percent_Identity=33.4841628959276, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI2367355, Length=224, Percent_Identity=32.1428571428571, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1789291, Length=200, Percent_Identity=27, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 47796; Mature: 47665

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAGALIPANSSLVRPPARIDNLDAWFAALTQPSALTELASLVACVLLAWLVVRVASRA
CCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RHSADESSILFGRRIVDGVLFPLLLLSFAYAAQALLARMFPLAVFKVVIPVLLALALIRL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVKVLQVAFKNAPLVRALERTISWLAWIAMVLWVSGLLPVILEELDGIKWKVGNSTLSVR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHH
TMLEGALTAGVVLLIALWISSALEARLLRSATGGELSLRKAVSNAARVVLMFVGLLLALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVGIDLTALSVLGGAIGVGVGFGLQKLAASYVSGFVMLAERSVRIGDNVRVDTFEGRITD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCEEEE
INARYTVVRAPSGRESIVPNEMFISNRIENLSLSDLRVLQSTVVSVGYDSDVALVMRLLA
CCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EAALSQPRVLRDPEPSVNLTNFGADGLEFTLNYWMTDPENGQQNLRSSINLEVLKALREN
HHHHCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHC
GIEIPYPQRVIHTRVSGVEGARTS
CCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ASAGALIPANSSLVRPPARIDNLDAWFAALTQPSALTELASLVACVLLAWLVVRVASRA
CCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RHSADESSILFGRRIVDGVLFPLLLLSFAYAAQALLARMFPLAVFKVVIPVLLALALIRL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVKVLQVAFKNAPLVRALERTISWLAWIAMVLWVSGLLPVILEELDGIKWKVGNSTLSVR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHH
TMLEGALTAGVVLLIALWISSALEARLLRSATGGELSLRKAVSNAARVVLMFVGLLLALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SVGIDLTALSVLGGAIGVGVGFGLQKLAASYVSGFVMLAERSVRIGDNVRVDTFEGRITD
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCEEEEECCCEEEE
INARYTVVRAPSGRESIVPNEMFISNRIENLSLSDLRVLQSTVVSVGYDSDVALVMRLLA
CCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EAALSQPRVLRDPEPSVNLTNFGADGLEFTLNYWMTDPENGQQNLRSSINLEVLKALREN
HHHHCCCCCCCCCCCCCEEECCCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHC
GIEIPYPQRVIHTRVSGVEGARTS
CCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]