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The map label for this gene is ppsA [H]

Identifier: 91788902

GI number: 91788902

Start: 3206156

End: 3208555

Strand: Reverse

Name: ppsA [H]

Synonym: Bpro_3042

Alternate gene names: 91788902

Gene position: 3208555-3206156 (Counterclockwise)

Preceding gene: 91788915

Following gene: 91788901

Centisome position: 61.7

GC content: 62.12

Gene sequence:

>2400_bases
ATGTCCAACCTGTTTGAGGCGACCGCCCTGGTCGTACCGTTTGAAAACCTGAGAATGACAGATGTCGAGGCCGTTGGCGG
CAAAAACGCCAGCCTCGGCGAGATGATCTCGCAGTTGCCCACCGGCCCGCAAGGCGTGCGGGTGCCTACGGGTTTTGCCA
CCACCGCGCACGCTTTCCGGGTGTTTCTGGCCTACGACGGTCTGGATAAAAAAATCAATGCGCTGCTCGATGCGCTCGAC
ACCGACGACGTGCGCGCGCTGGCCGAGGCCGGCGCCAAAATTCGCGCCCTGGTCGAAGCCCAGCCTTTCCCGCCGGACTT
CGAACAAGCCGTTCGCGGCAGCTTTGCCACCCTGAGCGCCGGAAATGCGCAGGCGAGCTTTGCGGTGCGTTCGTCCGCCA
CGGCCGAAGACCTGCCCGATGCTTCCTTTGCCGGCCAGCAGGAAACCTTCCTGAATGTCGTCGGCATTGAAGACGTGCTG
CACAAGATGAAAGAGGTGTTTGCCTCGCTCTATAACGACCGAGCCATCAGCTACCGCGTGCACAAGGGCTTTGCCCATGC
CGACGTGGCCCTGTCGGCCGGCGTGCAGCGCATGGTGCGCAGCGATCTGGGCGCGGCCGGCGTGATGTTCACCATCGACA
CCGAGAGCGGGTTTGAAGACGTGGTCTTCATCACCTCCAGTTACGGGTTGGGCGAAACGGTGGTGCAGGGCGCGGTGAAC
CCTGACGAGTTCTACGTGCACAAGCCCATGCTCGAGGCAGGCAATCGTGCCGTGATTCGTCGCAACCTGGGTTCCAAGCT
GCTGCAGATGGAGTTCACCACGGCCGAGGAGAAAAAGGCCGGCGGCAAACTCGTCAAGACCACCGACGTGCCTGCGGAAC
TGCGCAACCGCTATTCGCTGAGCGACGAAGACATCGAGCAGCTCGCCCGCTACGCAGTCATCATCGAAAAACACTATGGC
CGCGCCATGGACATCGAGTGGGGCAAGGACGGTACCGACGGCCAGCTCTACATCCTGCAGGCCCGCCCCGAGACGGTAAA
AAGCCAGTCCAAGGGCAAGGCCGAACAGCGCTACAAGCTCAAGGGCACCGGTACCGTGCTGGCCGAAGGCCGGGCGATCG
GCCAGAAGATCGGCACCGGCCCGGTTCGCATCGTGCACAACATCAGCGAGATGGACCAGGTTCAGCCTGGCGACATTCTG
GTGACCGACATGACCGACCCCAACTGGGAGCCGGTGATGAAACGCGCCAGCGCCATCGTGACCAACCGCGGCGGGCGCAC
CTGCCACGCGGCCATCATTGCGCGCGAGCTGGGCATTCCGGCGGTGGTCGGCTGTGGCGACGCCACCGAGCGGCTGGTGG
ACGGCATGCTGGTGACGGTCAGCTGCGCCGAAGGCGACACCGGCAACATCTACGACGGCTTGCTGGAAACCGAAGTGACC
GAGGTGCAGCGCGGCACCATGCCGCCTATCGACCTGAAGATCACGATGAACGTCGGCAACCCACAGCTGGCTTTTGATTT
TTGCCAGATTCCCAACCATGGCGTTGGCCTGGCGCGGCTGGAATTCATCATCAACAACAACATCGGTGTGCACCCCAAGG
CGATTCTCGACTACCCGAATGTCGATGCGGACCTGAAAAAGGCGGTGGAAAGCGTGGCGCGCGGCCATGCATCGCCACGC
GCTTTTTACGTGGACAAGGTGGCCGAAGGCATCGCCACCATTGGCGCGGCTTTCTGGCCCAAGCCGGTGATCGTGCGTCT
GTCGGATTTCAAGTCCAACGAATACCGCAAGCTGATTGGTGGTTCGCGTTACGAGCCCGAGGAGGAAAACCCGATGCTCG
GTTTCCGCGGCGCATCGCGCTATCTCAGCCCGGATTTTGGCGAAGCCTTTGCCATGGAATGTGAAGCGCTCAAGCGCGTG
CGCAACGACATGGGTCTGGTTAATGTTGAGGTCATGGTGCCTTTTGTCCGCACCCTCGGGCAGGCCAAAAAGGTGACCGA
TCTGCTGGCGCAGCATGGCCTCAAGCGCGGCGAGCGGGGCCTGCGCGTGATCATGATGTGCGAGGTGCCGAGCAATGCGA
TTCTGGCCGACCAGTTCCTCGAATATTTTGACGGTTTCTCGATTGGTTCGAACGACCTGACACAGCTGACGCTGGGACTG
GACCGTGATTCGGGCCTGGAGATCCTGGCATCGGACTTCGACGAGCGCGACCCGGCCGTCAAGGCTCTGCTGAGCCAGGC
CATCAGCGCCTGCAGGCGGCAGGGCAAATACGTGGGCATCTGCGGCCAGGGCCCCAGCGATCATCCGGACTTTGCACTCT
GGCTCAAGGAGCAGGGCATCGGCTCGATCTCGCTGAACCCGGACACCGTGATCGACACTTGGAAGCGCCTGGCCAACTGA

Upstream 100 bases:

>100_bases
ATTGTCACGCCATGCCCCGCCACAGCCCCTTCAAACAACAACAAGGTTGCTTGCCGCGCATGGTGAATTCCCCGGTTTTT
AAACCTCCCGGAGTATTTTC

Downstream 100 bases:

>100_bases
GGGAAAACCCCCGGTGCGCTGAGTGCGCGCGGTAAGCAACTGGCAAGCCCTCCGATGACAATCGGGGCATTTCCCCTAAA
TGCCATGCTTGCTGTTGTTA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MSNLFEATALVVPFENLRMTDVEAVGGKNASLGEMISQLPTGPQGVRVPTGFATTAHAFRVFLAYDGLDKKINALLDALD
TDDVRALAEAGAKIRALVEAQPFPPDFEQAVRGSFATLSAGNAQASFAVRSSATAEDLPDASFAGQQETFLNVVGIEDVL
HKMKEVFASLYNDRAISYRVHKGFAHADVALSAGVQRMVRSDLGAAGVMFTIDTESGFEDVVFITSSYGLGETVVQGAVN
PDEFYVHKPMLEAGNRAVIRRNLGSKLLQMEFTTAEEKKAGGKLVKTTDVPAELRNRYSLSDEDIEQLARYAVIIEKHYG
RAMDIEWGKDGTDGQLYILQARPETVKSQSKGKAEQRYKLKGTGTVLAEGRAIGQKIGTGPVRIVHNISEMDQVQPGDIL
VTDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLVDGMLVTVSCAEGDTGNIYDGLLETEVT
EVQRGTMPPIDLKITMNVGNPQLAFDFCQIPNHGVGLARLEFIINNNIGVHPKAILDYPNVDADLKKAVESVARGHASPR
AFYVDKVAEGIATIGAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGASRYLSPDFGEAFAMECEALKRV
RNDMGLVNVEVMVPFVRTLGQAKKVTDLLAQHGLKRGERGLRVIMMCEVPSNAILADQFLEYFDGFSIGSNDLTQLTLGL
DRDSGLEILASDFDERDPAVKALLSQAISACRRQGKYVGICGQGPSDHPDFALWLKEQGIGSISLNPDTVIDTWKRLAN

Sequences:

>Translated_799_residues
MSNLFEATALVVPFENLRMTDVEAVGGKNASLGEMISQLPTGPQGVRVPTGFATTAHAFRVFLAYDGLDKKINALLDALD
TDDVRALAEAGAKIRALVEAQPFPPDFEQAVRGSFATLSAGNAQASFAVRSSATAEDLPDASFAGQQETFLNVVGIEDVL
HKMKEVFASLYNDRAISYRVHKGFAHADVALSAGVQRMVRSDLGAAGVMFTIDTESGFEDVVFITSSYGLGETVVQGAVN
PDEFYVHKPMLEAGNRAVIRRNLGSKLLQMEFTTAEEKKAGGKLVKTTDVPAELRNRYSLSDEDIEQLARYAVIIEKHYG
RAMDIEWGKDGTDGQLYILQARPETVKSQSKGKAEQRYKLKGTGTVLAEGRAIGQKIGTGPVRIVHNISEMDQVQPGDIL
VTDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLVDGMLVTVSCAEGDTGNIYDGLLETEVT
EVQRGTMPPIDLKITMNVGNPQLAFDFCQIPNHGVGLARLEFIINNNIGVHPKAILDYPNVDADLKKAVESVARGHASPR
AFYVDKVAEGIATIGAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGASRYLSPDFGEAFAMECEALKRV
RNDMGLVNVEVMVPFVRTLGQAKKVTDLLAQHGLKRGERGLRVIMMCEVPSNAILADQFLEYFDGFSIGSNDLTQLTLGL
DRDSGLEILASDFDERDPAVKALLSQAISACRRQGKYVGICGQGPSDHPDFALWLKEQGIGSISLNPDTVIDTWKRLAN
>Mature_798_residues
SNLFEATALVVPFENLRMTDVEAVGGKNASLGEMISQLPTGPQGVRVPTGFATTAHAFRVFLAYDGLDKKINALLDALDT
DDVRALAEAGAKIRALVEAQPFPPDFEQAVRGSFATLSAGNAQASFAVRSSATAEDLPDASFAGQQETFLNVVGIEDVLH
KMKEVFASLYNDRAISYRVHKGFAHADVALSAGVQRMVRSDLGAAGVMFTIDTESGFEDVVFITSSYGLGETVVQGAVNP
DEFYVHKPMLEAGNRAVIRRNLGSKLLQMEFTTAEEKKAGGKLVKTTDVPAELRNRYSLSDEDIEQLARYAVIIEKHYGR
AMDIEWGKDGTDGQLYILQARPETVKSQSKGKAEQRYKLKGTGTVLAEGRAIGQKIGTGPVRIVHNISEMDQVQPGDILV
TDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLVDGMLVTVSCAEGDTGNIYDGLLETEVTE
VQRGTMPPIDLKITMNVGNPQLAFDFCQIPNHGVGLARLEFIINNNIGVHPKAILDYPNVDADLKKAVESVARGHASPRA
FYVDKVAEGIATIGAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGASRYLSPDFGEAFAMECEALKRVR
NDMGLVNVEVMVPFVRTLGQAKKVTDLLAQHGLKRGERGLRVIMMCEVPSNAILADQFLEYFDGFSIGSNDLTQLTLGLD
RDSGLEILASDFDERDPAVKALLSQAISACRRQGKYVGICGQGPSDHPDFALWLKEQGIGSISLNPDTVIDTWKRLAN

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=65.4040404040404, Blast_Score=1069, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=438, Percent_Identity=24.8858447488584, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788726, Length=193, Percent_Identity=32.1243523316062, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI48994992, Length=400, Percent_Identity=25.5, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1789193, Length=391, Percent_Identity=24.2966751918159, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564524, Length=244, Percent_Identity=28.6885245901639, Blast_Score=80, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86824; Mature: 86693

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLFEATALVVPFENLRMTDVEAVGGKNASLGEMISQLPTGPQGVRVPTGFATTAHAFR
CCCHHHHEEEEEECCCCEEEEHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHE
VFLAYDGLDKKINALLDALDTDDVRALAEAGAKIRALVEAQPFPPDFEQAVRGSFATLSA
EEHEECCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCEEEEEC
GNAQASFAVRSSATAEDLPDASFAGQQETFLNVVGIEDVLHKMKEVFASLYNDRAISYRV
CCCCEEEEEECCCCHHCCCCCCCCCCHHHHEEEECHHHHHHHHHHHHHHHHCCCEEEEEE
HKGFAHADVALSAGVQRMVRSDLGAAGVMFTIDTESGFEDVVFITSSYGLGETVVQGAVN
ECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCC
PDEFYVHKPMLEAGNRAVIRRNLGSKLLQMEFTTAEEKKAGGKLVKTTDVPAELRNRYSL
CCCEEEECHHHHCCCCEEEHHHHCCHHEEEEECCCHHHHCCCCEEEECCCCHHHHHCCCC
SDEDIEQLARYAVIIEKHYGRAMDIEWGKDGTDGQLYILQARPETVKSQSKGKAEQRYKL
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCCHHEEEE
KGTGTVLAEGRAIGQKIGTGPVRIVHNISEMDQVQPGDILVTDMTDPNWEPVMKRASAIV
CCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHH
TNRGGRTCHAAIIARELGIPAVVGCGDATERLVDGMLVTVSCAEGDTGNIYDGLLETEVT
CCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHH
EVQRGTMPPIDLKITMNVGNPQLAFDFCQIPNHGVGLARLEFIINNNIGVHPKAILDYPN
HHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHEECCCC
VDADLKKAVESVARGHASPRAFYVDKVAEGIATIGAAFWPKPVIVRLSDFKSNEYRKLIG
CCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHC
GSRYEPEEENPMLGFRGASRYLSPDFGEAFAMECEALKRVRNDMGLVNVEVMVPFVRTLG
CCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHH
QAKKVTDLLAQHGLKRGERGLRVIMMCEVPSNAILADQFLEYFDGFSIGSNDLTQLTLGL
HHHHHHHHHHHHCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEC
DRDSGLEILASDFDERDPAVKALLSQAISACRRQGKYVGICGQGPSDHPDFALWLKEQGI
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCC
GSISLNPDTVIDTWKRLAN
CEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
SNLFEATALVVPFENLRMTDVEAVGGKNASLGEMISQLPTGPQGVRVPTGFATTAHAFR
CCHHHHEEEEEECCCCEEEEHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHE
VFLAYDGLDKKINALLDALDTDDVRALAEAGAKIRALVEAQPFPPDFEQAVRGSFATLSA
EEHEECCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCEEEEEC
GNAQASFAVRSSATAEDLPDASFAGQQETFLNVVGIEDVLHKMKEVFASLYNDRAISYRV
CCCCEEEEEECCCCHHCCCCCCCCCCHHHHEEEECHHHHHHHHHHHHHHHHCCCEEEEEE
HKGFAHADVALSAGVQRMVRSDLGAAGVMFTIDTESGFEDVVFITSSYGLGETVVQGAVN
ECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCC
PDEFYVHKPMLEAGNRAVIRRNLGSKLLQMEFTTAEEKKAGGKLVKTTDVPAELRNRYSL
CCCEEEECHHHHCCCCEEEHHHHCCHHEEEEECCCHHHHCCCCEEEECCCCHHHHHCCCC
SDEDIEQLARYAVIIEKHYGRAMDIEWGKDGTDGQLYILQARPETVKSQSKGKAEQRYKL
CHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCHHHHHHHCCCCHHEEEE
KGTGTVLAEGRAIGQKIGTGPVRIVHNISEMDQVQPGDILVTDMTDPNWEPVMKRASAIV
CCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHH
TNRGGRTCHAAIIARELGIPAVVGCGDATERLVDGMLVTVSCAEGDTGNIYDGLLETEVT
CCCCCCCHHHHHHHHHCCCCEEEECCCHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHH
EVQRGTMPPIDLKITMNVGNPQLAFDFCQIPNHGVGLARLEFIINNNIGVHPKAILDYPN
HHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCHHEECCCC
VDADLKKAVESVARGHASPRAFYVDKVAEGIATIGAAFWPKPVIVRLSDFKSNEYRKLIG
CCHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHC
GSRYEPEEENPMLGFRGASRYLSPDFGEAFAMECEALKRVRNDMGLVNVEVMVPFVRTLG
CCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHH
QAKKVTDLLAQHGLKRGERGLRVIMMCEVPSNAILADQFLEYFDGFSIGSNDLTQLTLGL
HHHHHHHHHHHHCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEC
DRDSGLEILASDFDERDPAVKALLSQAISACRRQGKYVGICGQGPSDHPDFALWLKEQGI
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCC
GSISLNPDTVIDTWKRLAN
CEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]