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The map label for this gene is rlmE

Identifier: 91788714

GI number: 91788714

Start: 3008030

End: 3008773

Strand: Reverse

Name: rlmE

Synonym: Bpro_2853

Alternate gene names: 91788714

Gene position: 3008773-3008030 (Counterclockwise)

Preceding gene: 91788718

Following gene: 91788713

Centisome position: 57.86

GC content: 58.74

Gene sequence:

>744_bases
ATGAAAGTAAATCCCAAGAACAGCCCCAAGGACAACCTCAAAGACAGCCCAAAGGTGAGCGCCAGGGGCAAAAAGGTGAA
CAAAGCGTGGTTGCATGACCACATCAATGATCCTTACGTGAAACTGGCCCAAAAAGAGGGCTACCGCGCCCGGGCCGCCT
ACAAACTCAAGGAAATTGACGAAACCCTGGGCCTGATCAAGCCGGGTGATTGCGTGGTGGACCTGGGCAGCACGCCCGGC
GCCTGGAGCCAGTATGTGCGGCGCAAGCTGTCGCCCACGGGCGCTGCGGCGGGAGAACTGAATGGCCGCATCATCGGCCT
GGACATGCTGCCGATGGAGCCCATCGAGGGGGTGGCGTTCATCCAGGGCGATTTTCGGGAGCCCGAAGTGCTGCAGAAAC
TGGAGCAGGCTTTGGCGACGGACAAGGGTCAGGTCAAGGTTGACCTGGTTATTTCGGACATGGCCCCGAATCTTTCGGGC
ATTGAATCGGCGGATGCCGCACGCATTGTCCACCTGGTCGAGCTGGCGGTGGAATTTGCCCAGAACCGGATGAAACCGGA
CGGCACGCTGGTGGTCAAACTGTTTCACGGCAGCGGCTATGACGAGCTGGTCAAACTGTTCCGGGCCACCTTCAAGGTGG
TCAAACCCATGAAGCCCAAAGCCTCGCGTTCCAATTCTTCCGAAACCTTTTTGGTCGGCAAGGGGCTGAAAAAGCGGGCG
GAAACAGTGCCCGAACTGGCCTGA

Upstream 100 bases:

>100_bases
GATGGGCCTCGGCGCGGTGTTCTTTGCGCTGGGCGGGGGTAAGTTGAATTTGAGCCATGCCTCAATTATCGGTGGAGTCC
ACAGGAGGACAATAGCCAGC

Downstream 100 bases:

>100_bases
AAGCCGCTCGGAAGTGCCAAAAGTCACTTAAAACCGCTTCAAACCAAGGGGCGTACTCCGGTTAATGTCGTTTTTGTCCT
TGAAACGCCTAAAATCAGGC

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKVNPKNSPKDNLKDSPKVSARGKKVNKAWLHDHINDPYVKLAQKEGYRARAAYKLKEIDETLGLIKPGDCVVDLGSTPG
AWSQYVRRKLSPTGAAAGELNGRIIGLDMLPMEPIEGVAFIQGDFREPEVLQKLEQALATDKGQVKVDLVISDMAPNLSG
IESADAARIVHLVELAVEFAQNRMKPDGTLVVKLFHGSGYDELVKLFRATFKVVKPMKPKASRSNSSETFLVGKGLKKRA
ETVPELA

Sequences:

>Translated_247_residues
MKVNPKNSPKDNLKDSPKVSARGKKVNKAWLHDHINDPYVKLAQKEGYRARAAYKLKEIDETLGLIKPGDCVVDLGSTPG
AWSQYVRRKLSPTGAAAGELNGRIIGLDMLPMEPIEGVAFIQGDFREPEVLQKLEQALATDKGQVKVDLVISDMAPNLSG
IESADAARIVHLVELAVEFAQNRMKPDGTLVVKLFHGSGYDELVKLFRATFKVVKPMKPKASRSNSSETFLVGKGLKKRA
ETVPELA
>Mature_247_residues
MKVNPKNSPKDNLKDSPKVSARGKKVNKAWLHDHINDPYVKLAQKEGYRARAAYKLKEIDETLGLIKPGDCVVDLGSTPG
AWSQYVRRKLSPTGAAAGELNGRIIGLDMLPMEPIEGVAFIQGDFREPEVLQKLEQALATDKGQVKVDLVISDMAPNLSG
IESADAARIVHLVELAVEFAQNRMKPDGTLVVKLFHGSGYDELVKLFRATFKVVKPMKPKASRSNSSETFLVGKGLKKRA
ETVPELA

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=33.6405529953917, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI29029591, Length=211, Percent_Identity=32.2274881516588, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI29029589, Length=211, Percent_Identity=32.2274881516588, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI7110661, Length=211, Percent_Identity=32.2274881516588, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI194097365, Length=201, Percent_Identity=31.3432835820896, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=46.6346153846154, Blast_Score=190, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17553474, Length=222, Percent_Identity=34.2342342342342, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17554650, Length=196, Percent_Identity=31.6326530612245, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=202, Percent_Identity=32.6732673267327, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71987561, Length=202, Percent_Identity=32.6732673267327, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17553860, Length=196, Percent_Identity=33.1632653061224, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=212, Percent_Identity=31.6037735849057, Blast_Score=118, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319796, Length=202, Percent_Identity=35.6435643564356, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321302, Length=98, Percent_Identity=40.8163265306122, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356387, Length=225, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24647580, Length=197, Percent_Identity=33.502538071066, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI18859957, Length=199, Percent_Identity=29.6482412060301, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648639, Length=198, Percent_Identity=30.3030303030303, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_POLSJ (Q129M4)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549666.1
- ProteinModelPortal:   Q129M4
- SMR:   Q129M4
- STRING:   Q129M4
- GeneID:   4014503
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2853
- NMPDR:   fig|296591.1.peg.5444
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q129M4
- ProtClustDB:   CLSK951379
- BioCyc:   PSP296591:BPRO_2853-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 27072; Mature: 27072

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: ACT_SITE 193-193 BINDING 80-80 BINDING 82-82 BINDING 108-108 BINDING 124-124 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNPKNSPKDNLKDSPKVSARGKKVNKAWLHDHINDPYVKLAQKEGYRARAAYKLKEID
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
ETLGLIKPGDCVVDLGSTPGAWSQYVRRKLSPTGAAAGELNGRIIGLDMLPMEPIEGVAF
HHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEE
IQGDFREPEVLQKLEQALATDKGQVKVDLVISDMAPNLSGIESADAARIVHLVELAVEFA
EECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QNRMKPDGTLVVKLFHGSGYDELVKLFRATFKVVKPMKPKASRSNSSETFLVGKGLKKRA
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHH
ETVPELA
HCCCCCC
>Mature Secondary Structure
MKVNPKNSPKDNLKDSPKVSARGKKVNKAWLHDHINDPYVKLAQKEGYRARAAYKLKEID
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHH
ETLGLIKPGDCVVDLGSTPGAWSQYVRRKLSPTGAAAGELNGRIIGLDMLPMEPIEGVAF
HHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEE
IQGDFREPEVLQKLEQALATDKGQVKVDLVISDMAPNLSGIESADAARIVHLVELAVEFA
EECCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QNRMKPDGTLVVKLFHGSGYDELVKLFRATFKVVKPMKPKASRSNSSETFLVGKGLKKRA
HHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHH
ETVPELA
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA