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The map label for this gene is ilvI [H]

Identifier: 91788186

GI number: 91788186

Start: 2410211

End: 2411995

Strand: Direct

Name: ilvI [H]

Synonym: Bpro_2317

Alternate gene names: 91788186

Gene position: 2410211-2411995 (Clockwise)

Preceding gene: 91788179

Following gene: 91788187

Centisome position: 46.35

GC content: 59.44

Gene sequence:

>1785_bases
ATGGAAGTCTCCAAGGCGGAAATTGCTTCCGCGGCAGCCGCGTCTGCCAACACAAACGCAAATTCTCAAGAACTCCGGGG
CGCAGAAATCCTCGTGAAGTCGCTTCAGGCCGAGGGTGTCAAGTACATCTGGGGCTATCCGGGCGGCGCAGTTCTCCACA
TCTACGATGCTTTTTACAAGCAGGACTCGATCCAGCACATCCTGGTGCGCCACGAACAGGCTGCGGTGCATGCGGCCGAC
GGTTATGCCCGTGCCACGGGGGATGTCGGTGTCGCGCTGGTTACCTCCGGCCCGGGCGTAACCAATGCCGTGACCGGCAT
CGCCACGGCCTATATGGACAGCATCCCGATGGTGATCATCACCGGCCAGGTGCCAACCCACGCTATTGGCCTGGATGCTT
TTCAGGAGTGCGACACCGTCGGCATTACCCGTCCTATCGTCAAGCACAACTTCCTGGTCAAGGACGTGCGCCAGATGGCC
GACACGCTTAAAAAGGCATTCCATATCGCCCGCAGCGGCCGGCCGGGCCCGGTGGTGGTTGACATCCCCAAAGACGTGTC
GTTCAACAAGACGCTGTACACCGGTTATCCGCAGAGCGTCGAAATGCGTTCCTACAACCCGGTGCGCAAGGGACATGGCG
GGCAGATTCGCAAGGCACTGCAACTGCTGATGTCGGCCAAGCGGCCATACATCTACACCGGTGGTGGCGTGCTGCTGAGC
AATGCCTCGCAGGAGCTGCGCACGCTGGTGGACATGCTGGGCTATCCCTGCACCAACACGCTGATGGGGTTGGGCGCCTA
TCCGGCCAGCGACAAAAAGTTTCTCGGCATGCTGGGAATGCACGGCACCATTGAGGCCAACAATGCGATGCAGCACTGCG
ATGTATTGCTGGCAGTAGGGGCTCGTTTTGACGACCGCGTGATTGGCAACCCCAAGCACTTTGCGCAAAACGAGCGCAAG
ATCATTCATGTCGACATCGACCCTTCGAGCATTTCCAAGCGCGTCAAGGTCGATATCCCCATCGTGGGCGATGTCAAGGA
CGTGCTGACCGAGCTGATCAGCATGATCAGGGAGTCCGGTCTCAAGCCCGATGCCAATGCATTGGGCGGCTGGTGGGACA
CCATTGAAGGCTGGCGCAAGCGCGACTGCATGAAGTACAGCATGGGCAAGGGCGATGTGATCAAGCCCCAGCATGTGATC
GAGACGCTCTGGAACCTGACCAAGGACGCCGATACCTACATCACGTCGGATGTGGGCCAGCACCAGATGTGGGCCGCGCA
GTACTACAAGTTTGACGAGCCGCGGCGCTGGATCAATTCGGGTGGATTGGGGACCATGGGCGTCGGCATTCCCTATGCCA
TGGGCATCAAGCTGGCCAAACCCGACTCCGAGGTGTTCTGCATCACCGGGGAGGGCTCGGTGCAGATGTGTATCCAGGAG
CTTTCGACCTGCCTGCAGTACAACACGCCGATCAAGATCGTGTCGCTCAACAACCGCTATCTGGGCATGGTTCGCCAGTG
GCAGGAAGTGGAATACGCCGGGCGTTACAGCAGCAGCTACATGGACGCGCTGCCCAATTTCGTGAAGCTGGCCGAAGCCT
ATGGGCACGTCGGCATGCTGATCGAAAAACCGCAGGATGTGGAGCCAGCGCTTCGTGAGGCCCGCAAGCTCAAGGACCGC
ACGGTGTTCATGGACTTCCGCACGGATCCCACCGAGAACGTGTTCCCGATGGTGCAAGCCGGCAAGGGCATTACGGAAAT
GCTGCTGGGCTCTGAAGACCTGTAA

Upstream 100 bases:

>100_bases
TTCTGCTTATGGTTCTGGCCTCAAGTCTCAACGCTGCTTCACAAGAGTGGGCGAAGAGGCACCCAAGGTATTTCGTTAGA
GAAATTCACAAAGGTTCATC

Downstream 100 bases:

>100_bases
ACCCGCACCGTTCTATTTATCAACCGTCAACTGACGAATCTATTGTCTGCCAAGCCTGCGCATTACCGTGCGCGGGGGAG
GGCAACGAAAAGAGGAATCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MEVSKAEIASAAAASANTNANSQELRGAEILVKSLQAEGVKYIWGYPGGAVLHIYDAFYKQDSIQHILVRHEQAAVHAAD
GYARATGDVGVALVTSGPGVTNAVTGIATAYMDSIPMVIITGQVPTHAIGLDAFQECDTVGITRPIVKHNFLVKDVRQMA
DTLKKAFHIARSGRPGPVVVDIPKDVSFNKTLYTGYPQSVEMRSYNPVRKGHGGQIRKALQLLMSAKRPYIYTGGGVLLS
NASQELRTLVDMLGYPCTNTLMGLGAYPASDKKFLGMLGMHGTIEANNAMQHCDVLLAVGARFDDRVIGNPKHFAQNERK
IIHVDIDPSSISKRVKVDIPIVGDVKDVLTELISMIRESGLKPDANALGGWWDTIEGWRKRDCMKYSMGKGDVIKPQHVI
ETLWNLTKDADTYITSDVGQHQMWAAQYYKFDEPRRWINSGGLGTMGVGIPYAMGIKLAKPDSEVFCITGEGSVQMCIQE
LSTCLQYNTPIKIVSLNNRYLGMVRQWQEVEYAGRYSSSYMDALPNFVKLAEAYGHVGMLIEKPQDVEPALREARKLKDR
TVFMDFRTDPTENVFPMVQAGKGITEMLLGSEDL

Sequences:

>Translated_594_residues
MEVSKAEIASAAAASANTNANSQELRGAEILVKSLQAEGVKYIWGYPGGAVLHIYDAFYKQDSIQHILVRHEQAAVHAAD
GYARATGDVGVALVTSGPGVTNAVTGIATAYMDSIPMVIITGQVPTHAIGLDAFQECDTVGITRPIVKHNFLVKDVRQMA
DTLKKAFHIARSGRPGPVVVDIPKDVSFNKTLYTGYPQSVEMRSYNPVRKGHGGQIRKALQLLMSAKRPYIYTGGGVLLS
NASQELRTLVDMLGYPCTNTLMGLGAYPASDKKFLGMLGMHGTIEANNAMQHCDVLLAVGARFDDRVIGNPKHFAQNERK
IIHVDIDPSSISKRVKVDIPIVGDVKDVLTELISMIRESGLKPDANALGGWWDTIEGWRKRDCMKYSMGKGDVIKPQHVI
ETLWNLTKDADTYITSDVGQHQMWAAQYYKFDEPRRWINSGGLGTMGVGIPYAMGIKLAKPDSEVFCITGEGSVQMCIQE
LSTCLQYNTPIKIVSLNNRYLGMVRQWQEVEYAGRYSSSYMDALPNFVKLAEAYGHVGMLIEKPQDVEPALREARKLKDR
TVFMDFRTDPTENVFPMVQAGKGITEMLLGSEDL
>Mature_594_residues
MEVSKAEIASAAAASANTNANSQELRGAEILVKSLQAEGVKYIWGYPGGAVLHIYDAFYKQDSIQHILVRHEQAAVHAAD
GYARATGDVGVALVTSGPGVTNAVTGIATAYMDSIPMVIITGQVPTHAIGLDAFQECDTVGITRPIVKHNFLVKDVRQMA
DTLKKAFHIARSGRPGPVVVDIPKDVSFNKTLYTGYPQSVEMRSYNPVRKGHGGQIRKALQLLMSAKRPYIYTGGGVLLS
NASQELRTLVDMLGYPCTNTLMGLGAYPASDKKFLGMLGMHGTIEANNAMQHCDVLLAVGARFDDRVIGNPKHFAQNERK
IIHVDIDPSSISKRVKVDIPIVGDVKDVLTELISMIRESGLKPDANALGGWWDTIEGWRKRDCMKYSMGKGDVIKPQHVI
ETLWNLTKDADTYITSDVGQHQMWAAQYYKFDEPRRWINSGGLGTMGVGIPYAMGIKLAKPDSEVFCITGEGSVQMCIQE
LSTCLQYNTPIKIVSLNNRYLGMVRQWQEVEYAGRYSSSYMDALPNFVKLAEAYGHVGMLIEKPQDVEPALREARKLKDR
TVFMDFRTDPTENVFPMVQAGKGITEMLLGSEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=25.3940455341506, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI21361361, Length=571, Percent_Identity=26.7950963222417, Blast_Score=172, Evalue=6e-43,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=57.4171029668412, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=576, Percent_Identity=40.625, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=550, Percent_Identity=33.6363636363636, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI1787096, Length=570, Percent_Identity=28.4210526315789, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1788716, Length=565, Percent_Identity=24.7787610619469, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=586, Percent_Identity=26.1092150170648, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=586, Percent_Identity=26.1092150170648, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=524, Percent_Identity=27.2900763358779, Blast_Score=148, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=602, Percent_Identity=41.3621262458472, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=25.360824742268, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922626, Length=577, Percent_Identity=26.1698440207972, Blast_Score=179, Evalue=5e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65343; Mature: 65343

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVSKAEIASAAAASANTNANSQELRGAEILVKSLQAEGVKYIWGYPGGAVLHIYDAFYK
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHC
QDSIQHILVRHEQAAVHAADGYARATGDVGVALVTSGPGVTNAVTGIATAYMDSIPMVII
CCCHHHHHHHHHHHHEEECCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
TGQVPTHAIGLDAFQECDTVGITRPIVKHNFLVKDVRQMADTLKKAFHIARSGRPGPVVV
ECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
DIPKDVSFNKTLYTGYPQSVEMRSYNPVRKGHGGQIRKALQLLMSAKRPYIYTGGGVLLS
ECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEC
NASQELRTLVDMLGYPCTNTLMGLGAYPASDKKFLGMLGMHGTIEANNAMQHCDVLLAVG
CCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHC
ARFDDRVIGNPKHFAQNERKIIHVDIDPSSISKRVKVDIPIVGDVKDVLTELISMIRESG
CCCCCCCCCCCHHHCCCCCEEEEEECCHHHCCCEEEEECCEECCHHHHHHHHHHHHHHCC
LKPDANALGGWWDTIEGWRKRDCMKYSMGKGDVIKPQHVIETLWNLTKDADTYITSDVGQ
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCC
HQMWAAQYYKFDEPRRWINSGGLGTMGVGIPYAMGIKLAKPDSEVFCITGEGSVQMCIQE
HHHHHHHHHCCCCHHHHHCCCCCCEECCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHH
LSTCLQYNTPIKIVSLNNRYLGMVRQWQEVEYAGRYSSSYMDALPNFVKLAEAYGHVGML
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
IEKPQDVEPALREARKLKDRTVFMDFRTDPTENVFPMVQAGKGITEMLLGSEDL
ECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MEVSKAEIASAAAASANTNANSQELRGAEILVKSLQAEGVKYIWGYPGGAVLHIYDAFYK
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEHHHHHHC
QDSIQHILVRHEQAAVHAADGYARATGDVGVALVTSGPGVTNAVTGIATAYMDSIPMVII
CCCHHHHHHHHHHHHEEECCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEE
TGQVPTHAIGLDAFQECDTVGITRPIVKHNFLVKDVRQMADTLKKAFHIARSGRPGPVVV
ECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
DIPKDVSFNKTLYTGYPQSVEMRSYNPVRKGHGGQIRKALQLLMSAKRPYIYTGGGVLLS
ECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEC
NASQELRTLVDMLGYPCTNTLMGLGAYPASDKKFLGMLGMHGTIEANNAMQHCDVLLAVG
CCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHC
ARFDDRVIGNPKHFAQNERKIIHVDIDPSSISKRVKVDIPIVGDVKDVLTELISMIRESG
CCCCCCCCCCCHHHCCCCCEEEEEECCHHHCCCEEEEECCEECCHHHHHHHHHHHHHHCC
LKPDANALGGWWDTIEGWRKRDCMKYSMGKGDVIKPQHVIETLWNLTKDADTYITSDVGQ
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCC
HQMWAAQYYKFDEPRRWINSGGLGTMGVGIPYAMGIKLAKPDSEVFCITGEGSVQMCIQE
HHHHHHHHHCCCCHHHHHCCCCCCEECCCCHHHHCEEEECCCCCEEEEECCCHHHHHHHH
LSTCLQYNTPIKIVSLNNRYLGMVRQWQEVEYAGRYSSSYMDALPNFVKLAEAYGHVGML
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEE
IEKPQDVEPALREARKLKDRTVFMDFRTDPTENVFPMVQAGKGITEMLLGSEDL
ECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]