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The map label for this gene is pstS [H]

Identifier: 91788123

GI number: 91788123

Start: 2339064

End: 2340107

Strand: Direct

Name: pstS [H]

Synonym: Bpro_2252

Alternate gene names: 91788123

Gene position: 2339064-2340107 (Clockwise)

Preceding gene: 91788118

Following gene: 91788124

Centisome position: 44.98

GC content: 57.76

Gene sequence:

>1044_bases
ATGAAATTGAGTTTCAAAGCTTCTGCGACTGCTGTGATGGGTGTTGCCATCATGTCGTTTGCCACGTTGTCGGCCGCGCA
GGATGTCACGGGCGCTGGAGCCAGTTTCCCCGCTCCGCTGTATTCCAAATGGGCTGCTGACTACAACAAGGCCACCAGCA
TCAAGATTAATTACCAGTCGGTCGGCTCTGGCGCGGGTTTGCGGCAGATTGAGGCCAAGACCGTGGACTTTGGCGCATCA
GACGCTCCCCTGAAGGATGAAGAGCTCGCCAAAAAAGGGCTGGTGCAGTTTCCGACCGTGATTGGTGGCGTGGTTCCTGT
GGTCAATATCAAGGGCGTCGCTCCGGGCCAACTGAAGCTGAGCGGTCAGGTCCTGGGTGACATCTACCTGGGCAAGATCA
CCAAGTGGACCGACCCCGCCATCAAGGCGATGAATCCTGCACTGGCCTTGCCGGATGCGGCCATCGCGCCGGTCCGCCGA
GCTGATGGTTCAGGTACCAGCTTCATTTTCACCAATTATTTGAGCAAGGCCAACGCCGAGTGGAAGGCCAAAGTGGGTGA
AGGTACGGCGGTGAACTGGCCGGCGGGTGCTGGCGGCAAGGGTAACGAGGGCGTCGCGGCGTTTGTCGGTCGCCTGCCCA
ACTCGATTGGCTATGTGGAGTACGCCTATGTCAAGCAGAACAAGATGACGTTTGTCCAGCTGAAGAATGCTGCCGGCAAT
TTCGTGTCGCCGGATGATGCGGCATTCAAGGCTGCTGCTGCAGGCGCCGACTGGTCCAAGAGTTTTTACCAGATACTGAC
TGAGCAGCCCGGCAAGGATGCATGGCCTATCACGGGCGCCACCTTTATCCTGATGCAGAAGGCGCAGGACAAGCCCGCGC
AGGCCGCTGCCTCGCTCAAATTCTTCGAGTGGGCCTATAAGAACGGCGACAAGATCGCTGACGACCTGGACTATGTGCCC
ATGCCCGCCAGCGTGAAGACGATCATCGAAAAATCGTGGGCTGACATCAAGGACACGTCGGGCAAGGCTGTTGCTTACAA
ATAA

Upstream 100 bases:

>100_bases
GGATTTCCATGCATTGCCCCGGGAGGGTGTCTGTCACATCGTTGTCACACAAGTTTTTTACAGTTCGTGCATCTTTCCTA
CCAACCCCAAAGGTGTTCCG

Downstream 100 bases:

>100_bases
GCCTGCTGCTTGGCCTGTTTTCCCTGAAAGTGACGACGAATCATGGGGGTTTTATAAGTGTCCTCTACACTTCCGGCTAG
CCGGGCCGCTTTTGATCACC

Product: periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKLSFKASATAVMGVAIMSFATLSAAQDVTGAGASFPAPLYSKWAADYNKATSIKINYQSVGSGAGLRQIEAKTVDFGAS
DAPLKDEELAKKGLVQFPTVIGGVVPVVNIKGVAPGQLKLSGQVLGDIYLGKITKWTDPAIKAMNPALALPDAAIAPVRR
ADGSGTSFIFTNYLSKANAEWKAKVGEGTAVNWPAGAGGKGNEGVAAFVGRLPNSIGYVEYAYVKQNKMTFVQLKNAAGN
FVSPDDAAFKAAAAGADWSKSFYQILTEQPGKDAWPITGATFILMQKAQDKPAQAAASLKFFEWAYKNGDKIADDLDYVP
MPASVKTIIEKSWADIKDTSGKAVAYK

Sequences:

>Translated_347_residues
MKLSFKASATAVMGVAIMSFATLSAAQDVTGAGASFPAPLYSKWAADYNKATSIKINYQSVGSGAGLRQIEAKTVDFGAS
DAPLKDEELAKKGLVQFPTVIGGVVPVVNIKGVAPGQLKLSGQVLGDIYLGKITKWTDPAIKAMNPALALPDAAIAPVRR
ADGSGTSFIFTNYLSKANAEWKAKVGEGTAVNWPAGAGGKGNEGVAAFVGRLPNSIGYVEYAYVKQNKMTFVQLKNAAGN
FVSPDDAAFKAAAAGADWSKSFYQILTEQPGKDAWPITGATFILMQKAQDKPAQAAASLKFFEWAYKNGDKIADDLDYVP
MPASVKTIIEKSWADIKDTSGKAVAYK
>Mature_347_residues
MKLSFKASATAVMGVAIMSFATLSAAQDVTGAGASFPAPLYSKWAADYNKATSIKINYQSVGSGAGLRQIEAKTVDFGAS
DAPLKDEELAKKGLVQFPTVIGGVVPVVNIKGVAPGQLKLSGQVLGDIYLGKITKWTDPAIKAMNPALALPDAAIAPVRR
ADGSGTSFIFTNYLSKANAEWKAKVGEGTAVNWPAGAGGKGNEGVAAFVGRLPNSIGYVEYAYVKQNKMTFVQLKNAAGN
FVSPDDAAFKAAAAGADWSKSFYQILTEQPGKDAWPITGATFILMQKAQDKPAQAAASLKFFEWAYKNGDKIADDLDYVP
MPASVKTIIEKSWADIKDTSGKAVAYK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=326, Percent_Identity=60.4294478527607, Blast_Score=393, Evalue=1e-111,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36539; Mature: 36539

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSFKASATAVMGVAIMSFATLSAAQDVTGAGASFPAPLYSKWAADYNKATSIKINYQS
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEE
VGSGAGLRQIEAKTVDFGASDAPLKDEELAKKGLVQFPTVIGGVVPVVNIKGVAPGQLKL
CCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHCCEEEEEECCCCCCCEEE
SGQVLGDIYLGKITKWTDPAIKAMNPALALPDAAIAPVRRADGSGTSFIFTNYLSKANAE
CCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCHHHCCHHCCCCCCCEEEEEHHHHHCCCC
WKAKVGEGTAVNWPAGAGGKGNEGVAAFVGRLPNSIGYVEYAYVKQNKMTFVQLKNAAGN
EEEECCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCC
FVSPDDAAFKAAAAGADWSKSFYQILTEQPGKDAWPITGATFILMQKAQDKPAQAAASLK
CCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
FFEWAYKNGDKIADDLDYVPMPASVKTIIEKSWADIKDTSGKAVAYK
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEC
>Mature Secondary Structure
MKLSFKASATAVMGVAIMSFATLSAAQDVTGAGASFPAPLYSKWAADYNKATSIKINYQS
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEEE
VGSGAGLRQIEAKTVDFGASDAPLKDEELAKKGLVQFPTVIGGVVPVVNIKGVAPGQLKL
CCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHCCEEEEEECCCCCCCEEE
SGQVLGDIYLGKITKWTDPAIKAMNPALALPDAAIAPVRRADGSGTSFIFTNYLSKANAE
CCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCHHHCCHHCCCCCCCEEEEEHHHHHCCCC
WKAKVGEGTAVNWPAGAGGKGNEGVAAFVGRLPNSIGYVEYAYVKQNKMTFVQLKNAAGN
EEEECCCCCEECCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCC
FVSPDDAAFKAAAAGADWSKSFYQILTEQPGKDAWPITGATFILMQKAQDKPAQAAASLK
CCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
FFEWAYKNGDKIADDLDYVPMPASVKTIIEKSWADIKDTSGKAVAYK
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6321434; 6365894; 7686882; 9278503; 9298646; 2215649; 9228942; 9671506 [H]