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The map label for this gene is gcvH [H]

Identifier: 91787746

GI number: 91787746

Start: 1928908

End: 1929276

Strand: Reverse

Name: gcvH [H]

Synonym: Bpro_1867

Alternate gene names: 91787746

Gene position: 1929276-1928908 (Counterclockwise)

Preceding gene: 91787747

Following gene: 91787745

Centisome position: 37.1

GC content: 62.6

Gene sequence:

>369_bases
ATGACTGTCAAATACACCGAAGACCACGAATGGCTCAAGATTGACGGCGACACCGCCACCGTGGGCATCACGGTTCATGC
GCAGGACGCGCTGGGCGATGTCGTGTTTGTCGACCTGCCTGCCGTCGGCGCCACCCTGGCGCAGAAGGAAGCCGCCGGCG
TGGTGGAATCAGTCAAGGCCGCCGCAGACGTCTACATGCCCGTGAGCGGCGAGGTCGTTGAAGTCAACGAAGCCCTGCGC
AACGACCCATCGCTGGCCAACAGCGACCCGCTGGGCGCCGGCTGGTTCTTCAAGATCAGGCTGTCTGACGCCTCGCAGCT
GGACGCCCTGATGGACGAAACCAGCTACACCCAGTTTTCCGCAGGTTAA

Upstream 100 bases:

>100_bases
GCTGAGCAACCTACCGACTTTCACTGCCGCCTTTCAAGTTATTCACGTTACTTTTACGTTAACTTGCTGAATCCTTTTCA
CTGAACTTCTGGAGAACCTC

Downstream 100 bases:

>100_bases
TGGCCCCCACGCTTGCCACTTCGTGTGCTGCGCTGCCCCCCGAGGGGGCTGTGCTTGCTTGGGTCGGCCCTGCGCGGCGC
ACGTCCGTCTGTCTGTCTTT

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MTVKYTEDHEWLKIDGDTATVGITVHAQDALGDVVFVDLPAVGATLAQKEAAGVVESVKAAADVYMPVSGEVVEVNEALR
NDPSLANSDPLGAGWFFKIRLSDASQLDALMDETSYTQFSAG

Sequences:

>Translated_122_residues
MTVKYTEDHEWLKIDGDTATVGITVHAQDALGDVVFVDLPAVGATLAQKEAAGVVESVKAAADVYMPVSGEVVEVNEALR
NDPSLANSDPLGAGWFFKIRLSDASQLDALMDETSYTQFSAG
>Mature_121_residues
TVKYTEDHEWLKIDGDTATVGITVHAQDALGDVVFVDLPAVGATLAQKEAAGVVESVKAAADVYMPVSGEVVEVNEALRN
DPSLANSDPLGAGWFFKIRLSDASQLDALMDETSYTQFSAG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=48.2758620689655, Blast_Score=122, Evalue=5e-29,
Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=48.2758620689655, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI1789271, Length=115, Percent_Identity=57.3913043478261, Blast_Score=140, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=41.8803418803419, Blast_Score=118, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=41.7391304347826, Blast_Score=111, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=118, Percent_Identity=43.2203389830509, Blast_Score=109, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=47.8632478632479, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 12932; Mature: 12801

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKYTEDHEWLKIDGDTATVGITVHAQDALGDVVFVDLPAVGATLAQKEAAGVVESVKA
CCEEECCCCCEEEEECCEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
AADVYMPVSGEVVEVNEALRNDPSLANSDPLGAGWFFKIRLSDASQLDALMDETSYTQFS
HHEEEECCCCCEEEECHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCEEEC
AG
CC
>Mature Secondary Structure 
TVKYTEDHEWLKIDGDTATVGITVHAQDALGDVVFVDLPAVGATLAQKEAAGVVESVKA
CEEECCCCCEEEEECCEEEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
AADVYMPVSGEVVEVNEALRNDPSLANSDPLGAGWFFKIRLSDASQLDALMDETSYTQFS
HHEEEECCCCCEEEECHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCCEEEC
AG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]