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The map label for this gene is pit [H]

Identifier: 91787569

GI number: 91787569

Start: 1739092

End: 1740102

Strand: Direct

Name: pit [H]

Synonym: Bpro_1687

Alternate gene names: 91787569

Gene position: 1739092-1740102 (Clockwise)

Preceding gene: 91787568

Following gene: 91787572

Centisome position: 33.44

GC content: 61.92

Gene sequence:

>1011_bases
ATGCAAACCGTACAGACCGCCCTGTGGGTCGTAGTGCTGCTGGTGTTCCTGGCCATCGCCTTTGACTTCATGAATGGCTT
CCACGATGCAGCCAATTCGATCGCGACGGTGGTGTCCACCGGTGTGCTCAAGCCGGGCCAAGCCGTCGTCTTTGCGGCCG
CGTTTAACCTGATCGCCATTTTCGTCTTCCACCTCAGTGTGGCCGCCACCGTGGGCAAGGGCATTGTGCAGCCCGGTGTT
GTCGACACACACGTGGTGTTTGGCGCGCTGACCGGCGCCATCACCTGGAACTTCGTGACCTGGTATTACGGCATCCCGAG
CAGCTCGTCGCATGCCCTGATAGGCGGCATTGTGGGCGCGGTGATTGCCAAGGCCGGTGCTGGCTCCCTGGTTTCGGCGG
GCATCCTGAAGACGGTGCTTTTTATTTTCGTGTCGCCATTGCTGGGGTTCCTGCTCGGATCCCTGATGATGGTGCTGGTT
GCCTGGGTCTGTCGGCGCGCAACGCCGTCGAAAGTGGATGGCTGGTTTCGCCGCCTGCAGTTGGTGTCTGCCGGGGCCTA
CAGCCTGGGGCATGGCGGCAATGATGCGCAGAAAACCATAGGCATCATCTGGATGCTGCTGATTGCCACCGGCTATGCGT
CGGCGAGCGACGCCGCGCCCCCCGGCTGGACGATCGTGAGCTGCTACCTCGCCATTGCGGCGGGCACCATGTTTGGCGGC
TGGCGCATCGTCAAGACCATGGGCCAGAAAATTACCAAGCTCAAGCCCGTGGGCGGCTTTTGTGCTGAAACCGGCGGTGC
GCTGACGCTGTTCCTGGCGACCGGGCTTGGTATTCCTGTCTCAACCACGCACACCATCACCGGTGCCATCGTCGGTGTGG
GCTCCACGCAGCGCGCTTCTGCCGTGCGCTGGGGCGTCGCCGGCAACATTGTCTGGGCCTGGATTTTTACCATTCCCGCC
TCCGCCTTCGTGGCGGCCATCGGCTACTGGTTCAGCCTGCAGATTTTTTAA

Upstream 100 bases:

>100_bases
ATTTACGAGTTGCTCGAAACCATCACCGACAAATGCGAGGATGTGGCCAATCTCATCGAGGGCATCGTCCTCGAAAATTC
CTGATCGCTGGTCACCTGTT

Downstream 100 bases:

>100_bases
ACAATTTCCCGCAAGTCCGCCCCAAGCAAGTTCAGCCCCCGTGGAGGCATCCTTCGACAGGACGAACGGAATTCAGTGAA
TGAACGTTGGCCCCCAGCCG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MQTVQTALWVVVLLVFLAIAFDFMNGFHDAANSIATVVSTGVLKPGQAVVFAAAFNLIAIFVFHLSVAATVGKGIVQPGV
VDTHVVFGALTGAITWNFVTWYYGIPSSSSHALIGGIVGAVIAKAGAGSLVSAGILKTVLFIFVSPLLGFLLGSLMMVLV
AWVCRRATPSKVDGWFRRLQLVSAGAYSLGHGGNDAQKTIGIIWMLLIATGYASASDAAPPGWTIVSCYLAIAAGTMFGG
WRIVKTMGQKITKLKPVGGFCAETGGALTLFLATGLGIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWIFTIPA
SAFVAAIGYWFSLQIF

Sequences:

>Translated_336_residues
MQTVQTALWVVVLLVFLAIAFDFMNGFHDAANSIATVVSTGVLKPGQAVVFAAAFNLIAIFVFHLSVAATVGKGIVQPGV
VDTHVVFGALTGAITWNFVTWYYGIPSSSSHALIGGIVGAVIAKAGAGSLVSAGILKTVLFIFVSPLLGFLLGSLMMVLV
AWVCRRATPSKVDGWFRRLQLVSAGAYSLGHGGNDAQKTIGIIWMLLIATGYASASDAAPPGWTIVSCYLAIAAGTMFGG
WRIVKTMGQKITKLKPVGGFCAETGGALTLFLATGLGIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWIFTIPA
SAFVAAIGYWFSLQIF
>Mature_336_residues
MQTVQTALWVVVLLVFLAIAFDFMNGFHDAANSIATVVSTGVLKPGQAVVFAAAFNLIAIFVFHLSVAATVGKGIVQPGV
VDTHVVFGALTGAITWNFVTWYYGIPSSSSHALIGGIVGAVIAKAGAGSLVSAGILKTVLFIFVSPLLGFLLGSLMMVLV
AWVCRRATPSKVDGWFRRLQLVSAGAYSLGHGGNDAQKTIGIIWMLLIATGYASASDAAPPGWTIVSCYLAIAAGTMFGG
WRIVKTMGQKITKLKPVGGFCAETGGALTLFLATGLGIPVSTTHTITGAIVGVGSTQRASAVRWGVAGNIVWAWIFTIPA
SAFVAAIGYWFSLQIF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=153, Percent_Identity=37.2549019607843, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=35.5263157894737, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=36.8663594470046, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1789360, Length=284, Percent_Identity=30.2816901408451, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17539280, Length=163, Percent_Identity=31.9018404907975, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=29.0123456790123, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=28.8343558282209, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557328, Length=153, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI32566716, Length=167, Percent_Identity=27.5449101796407, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=164, Percent_Identity=28.0487804878049, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=165, Percent_Identity=30.3030303030303, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34960; Mature: 34960

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVQTALWVVVLLVFLAIAFDFMNGFHDAANSIATVVSTGVLKPGQAVVFAAAFNLIAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FVFHLSVAATVGKGIVQPGVVDTHVVFGALTGAITWNFVTWYYGIPSSSSHALIGGIVGA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCHHHHHHHHH
VIAKAGAGSLVSAGILKTVLFIFVSPLLGFLLGSLMMVLVAWVCRRATPSKVDGWFRRLQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LVSAGAYSLGHGGNDAQKTIGIIWMLLIATGYASASDAAPPGWTIVSCYLAIAAGTMFGG
HHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCH
WRIVKTMGQKITKLKPVGGFCAETGGALTLFLATGLGIPVSTTHTITGAIVGVGSTQRAS
HHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEHHCCCCCCCCCHHHHHHEEECCCCCHHH
AVRWGVAGNIVWAWIFTIPASAFVAAIGYWFSLQIF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEC
>Mature Secondary Structure
MQTVQTALWVVVLLVFLAIAFDFMNGFHDAANSIATVVSTGVLKPGQAVVFAAAFNLIAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FVFHLSVAATVGKGIVQPGVVDTHVVFGALTGAITWNFVTWYYGIPSSSSHALIGGIVGA
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCHHHHHHHHH
VIAKAGAGSLVSAGILKTVLFIFVSPLLGFLLGSLMMVLVAWVCRRATPSKVDGWFRRLQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LVSAGAYSLGHGGNDAQKTIGIIWMLLIATGYASASDAAPPGWTIVSCYLAIAAGTMFGG
HHHCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCH
WRIVKTMGQKITKLKPVGGFCAETGGALTLFLATGLGIPVSTTHTITGAIVGVGSTQRAS
HHHHHHHHHHHHHCCCCCCHHHCCCCCEEEEEHHCCCCCCCCCHHHHHHEEECCCCCHHH
AVRWGVAGNIVWAWIFTIPASAFVAAIGYWFSLQIF
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]