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The map label for this gene is dusB [H]

Identifier: 91787028

GI number: 91787028

Start: 1168379

End: 1169449

Strand: Direct

Name: dusB [H]

Synonym: Bpro_1130

Alternate gene names: 91787028

Gene position: 1168379-1169449 (Clockwise)

Preceding gene: 91787026

Following gene: 91787029

Centisome position: 22.47

GC content: 62.46

Gene sequence:

>1071_bases
ATGAACATTGGCCCCTACGCTTTGGCAAACAGACTGTTTGTTGCGCCCATGGCTGGTGTGACGGACCGGCCGTTTCGCCA
GCTGTGCAAAAAGCTGGGGGCGGGCTATGCGGTCAGCGAGATGGTGACCTCGCGCCGCGATTTGTGGGACAGCCTGAAGA
CTTCGCGCCGTGCCAACCATGCCGGCGAGGCTGCGCCGATTGCGGTGCAGATTGCCGGCACCGACGCGCAGATGATGGCC
GAAGCTGCGGCCTACAACGTGGACCGCGGCGCTCAAATCATCGACATCAACATGGGTTGCCCGGCCAAGAAGGTGTGCAA
CAAATGGGCCGGCTCGGCCCTGATGCAGGACGAAACGCTGGCGCTGCAGATTGTGGAGGCCGTCGTCGCCGCCTGCGCCC
CCCATGGCGTTCCTGTCACGCTCAAGATGCGCACCGGCTGGTGCCAGGCCCACAAAAACGCCCTGGCGATTGCGCGCGCG
GCTGAAAGCGCCGGCGTGCAGATGGTTACCGTGCATGGCCGCACGCGCGAGCAGGGATACAAGGGCTTGGCCGAATATGA
CACCATTGCCGCGGTGAAGTCGGCCTTGCGCATTCCGGTGGTGGCCAATGGCGATATCAGCAGTCCGGAAAAAGCCCGCG
ATGTGCTGGCTTACACCGGTGCCGATGCCGTGATGATTGGTCGTGCGGCCCAGGGACGGCCCTGGATTTTCCGCGAGGTG
GCGCATTTCCTGGCCACGGGCACGCATCTTGCGCCGCCGCTGGTCGCGGAAGTCAGACGCCTGTTGCTCGATCATTTGCT
GGATCACTATGATCTGTATGGCCAATACACCGGTGTGCGCACCGCACGCAAGCACATCGGCTGGTATGTCAAGACCTTGC
CGGGTGGTGAGGTGTTTCGCGCAAAGATGAATCTGCTGGAAGACTGCGAGGCACAGCTGCGCGCAGTCGGGGATTTTTTC
GAGGCGCTTGGCGACCGGATGGACCGCATTCCGGCAACTGGGGACGCCGGGAGCGGGCCGAGGCAGGCATGGATGAACAA
AGATAAAAATCAGGAGGAAGCTGCTGCATGA

Upstream 100 bases:

>100_bases
AGGGCGCTTACATATCCGTGTGCAAATTTCAATTGCTGAAATTTTAGGCAGCTACAATCATGCCTTCGGTTTTCCGCAAT
CAGCGCCCCTCATTTTTTCC

Downstream 100 bases:

>100_bases
GCAAAAAACACATAGAAGAGTGCGTACGCACCAGTCTCGAGGGCTATTTCAGGGACCTGCGCGGTACCGATCCCGATGGC
ATGTACGACATGATGGTCCG

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNIGPYALANRLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVTSRRDLWDSLKTSRRANHAGEAAPIAVQIAGTDAQMMA
EAAAYNVDRGAQIIDINMGCPAKKVCNKWAGSALMQDETLALQIVEAVVAACAPHGVPVTLKMRTGWCQAHKNALAIARA
AESAGVQMVTVHGRTREQGYKGLAEYDTIAAVKSALRIPVVANGDISSPEKARDVLAYTGADAVMIGRAAQGRPWIFREV
AHFLATGTHLAPPLVAEVRRLLLDHLLDHYDLYGQYTGVRTARKHIGWYVKTLPGGEVFRAKMNLLEDCEAQLRAVGDFF
EALGDRMDRIPATGDAGSGPRQAWMNKDKNQEEAAA

Sequences:

>Translated_356_residues
MNIGPYALANRLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVTSRRDLWDSLKTSRRANHAGEAAPIAVQIAGTDAQMMA
EAAAYNVDRGAQIIDINMGCPAKKVCNKWAGSALMQDETLALQIVEAVVAACAPHGVPVTLKMRTGWCQAHKNALAIARA
AESAGVQMVTVHGRTREQGYKGLAEYDTIAAVKSALRIPVVANGDISSPEKARDVLAYTGADAVMIGRAAQGRPWIFREV
AHFLATGTHLAPPLVAEVRRLLLDHLLDHYDLYGQYTGVRTARKHIGWYVKTLPGGEVFRAKMNLLEDCEAQLRAVGDFF
EALGDRMDRIPATGDAGSGPRQAWMNKDKNQEEAAA
>Mature_356_residues
MNIGPYALANRLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVTSRRDLWDSLKTSRRANHAGEAAPIAVQIAGTDAQMMA
EAAAYNVDRGAQIIDINMGCPAKKVCNKWAGSALMQDETLALQIVEAVVAACAPHGVPVTLKMRTGWCQAHKNALAIARA
AESAGVQMVTVHGRTREQGYKGLAEYDTIAAVKSALRIPVVANGDISSPEKARDVLAYTGADAVMIGRAAQGRPWIFREV
AHFLATGTHLAPPLVAEVRRLLLDHLLDHYDLYGQYTGVRTARKHIGWYVKTLPGGEVFRAKMNLLEDCEAQLRAVGDFF
EALGDRMDRIPATGDAGSGPRQAWMNKDKNQEEAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=33.8912133891213, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI239788462, Length=239, Percent_Identity=34.3096234309623, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=32.8888888888889, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=31.3304721030043, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI8923374, Length=338, Percent_Identity=26.3313609467456, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=54.8286604361371, Blast_Score=344, Evalue=4e-96,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=34.8547717842324, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=26.6025641025641, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=246, Percent_Identity=30.4878048780488, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=254, Percent_Identity=28.3464566929134, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=230, Percent_Identity=30.4347826086957, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=251, Percent_Identity=28.2868525896414, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=267, Percent_Identity=28.8389513108614, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=238, Percent_Identity=31.9327731092437, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=27.6315789473684, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=33.0472103004292, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=33.0472103004292, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=33.75, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI19921524, Length=244, Percent_Identity=30.327868852459, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45549423, Length=370, Percent_Identity=25.1351351351351, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38508; Mature: 38508

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGPYALANRLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVTSRRDLWDSLKTSRRANH
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AGEAAPIAVQIAGTDAQMMAEAAAYNVDRGAQIIDINMGCPAKKVCNKWAGSALMQDETL
CCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHCCHHH
ALQIVEAVVAACAPHGVPVTLKMRTGWCQAHKNALAIARAAESAGVQMVTVHGRTREQGY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHC
KGLAEYDTIAAVKSALRIPVVANGDISSPEKARDVLAYTGADAVMIGRAAQGRPWIFREV
CCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHH
AHFLATGTHLAPPLVAEVRRLLLDHLLDHYDLYGQYTGVRTARKHIGWYVKTLPGGEVFR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHH
AKMNLLEDCEAQLRAVGDFFEALGDRMDRIPATGDAGSGPRQAWMNKDKNQEEAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHCCC
>Mature Secondary Structure
MNIGPYALANRLFVAPMAGVTDRPFRQLCKKLGAGYAVSEMVTSRRDLWDSLKTSRRANH
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
AGEAAPIAVQIAGTDAQMMAEAAAYNVDRGAQIIDINMGCPAKKVCNKWAGSALMQDETL
CCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCHHHCCHHH
ALQIVEAVVAACAPHGVPVTLKMRTGWCQAHKNALAIARAAESAGVQMVTVHGRTREQGY
HHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHC
KGLAEYDTIAAVKSALRIPVVANGDISSPEKARDVLAYTGADAVMIGRAAQGRPWIFREV
CCHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHHHHH
AHFLATGTHLAPPLVAEVRRLLLDHLLDHYDLYGQYTGVRTARKHIGWYVKTLPGGEVFR
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHH
AKMNLLEDCEAQLRAVGDFFEALGDRMDRIPATGDAGSGPRQAWMNKDKNQEEAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]