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The map label for this gene is mraW

Identifier: 91786965

GI number: 91786965

Start: 1097273

End: 1098190

Strand: Direct

Name: mraW

Synonym: Bpro_1067

Alternate gene names: 91786965

Gene position: 1097273-1098190 (Clockwise)

Preceding gene: 91786964

Following gene: 91786966

Centisome position: 21.1

GC content: 61.33

Gene sequence:

>918_bases
GTGCAAGACACATGGACACACCGCACCGTCTTGTTGAACGAAGCCATAGCGGCGCTTCACATCAACCCGGACGGCCACTA
CATCGACGCGACTTTCGGTCGCGGCGGGCATTCGCGCCTCTTGTTGTCGCAGCTCTCGCCGCAAGGACGTCTCACGGCCT
TCGACAAGGACCTCGACGCCATCGCGGAAGCGCAAACCATTACCGACCCGCGGTTTTCAATTCGCCACGAAGGTTTCATG
CACCTGGGTCAACTGCCCGCTGCCAGCGCAGCGGGCGTGCTGATGGATCTTGGCGTGAGCTCGCCCCAGATCGACAACCC
GGTGCGCGGCTTTTCCTTTCGTTCCGAGGGACCTCTGGACATGCGCATGGACACCACGCGTGGCCAGAGCGTGGCCGAGT
GGCTGGCAGATGCATCCATAGAAACAATGGCGGAGGTGATTCGTGAATATGGCGAAGAACGGTTTGCTCAACAGGTTGCA
AAGGCGATTGATCGTCGCCGACAGGAACGGGGCCCTCTTCGAACCACCGCTGAGCTGGCCGAAGTCGTGGCTGGCGCGGT
CAAAACCCGCGAGCCGGGCAAGGACCCTGCAACGCGCACATTTCAGGCTCTTCGGATTTTCATCAACGCCGAGCTTGAAG
AGCTGCAACAGGCGTTAGAAGCTTCACTGAAAGTACTCCAACCGGGAGGCCGTCTGGTGGTGATCAGTTTTCATTCGCTC
GAAGACCGCATCGTCAAGCAATTCATCGCCGGGCAATCGCGCGAAGTGTTCGACCGTCGCGCCCCATTTGCCGCACCCAA
GGCCATGAGGCTCAAGGCGCTGGGCCGCATCAAGCCCAGCGAAGAGGAGATTGCCGCCAACCCCCGTTCCCGCAGCGCCG
TGATGCGGGTGGCCGAGCGCACCGGGGTGAGCGCATGA

Upstream 100 bases:

>100_bases
CGAGCTCTGGGACGCCGCGACTTATGCCGCCCAGGAGGCCGAGCAGATGAAGGGTGAAATGCCCGATGTCTTCAGAGACT
TTTCATTCTGAAAGAAGACG

Downstream 100 bases:

>100_bases
ACACCGCAAAACTACTCCAGCACGCACGCAACTCAGTGGGTGTGAGCATGAGTGCAGTACGCGCAGCGATCAGCGTGGGC
GCCAACGAACGCCGCGAAGG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MQDTWTHRTVLLNEAIAALHINPDGHYIDATFGRGGHSRLLLSQLSPQGRLTAFDKDLDAIAEAQTITDPRFSIRHEGFM
HLGQLPAASAAGVLMDLGVSSPQIDNPVRGFSFRSEGPLDMRMDTTRGQSVAEWLADASIETMAEVIREYGEERFAQQVA
KAIDRRRQERGPLRTTAELAEVVAGAVKTREPGKDPATRTFQALRIFINAELEELQQALEASLKVLQPGGRLVVISFHSL
EDRIVKQFIAGQSREVFDRRAPFAAPKAMRLKALGRIKPSEEEIAANPRSRSAVMRVAERTGVSA

Sequences:

>Translated_305_residues
MQDTWTHRTVLLNEAIAALHINPDGHYIDATFGRGGHSRLLLSQLSPQGRLTAFDKDLDAIAEAQTITDPRFSIRHEGFM
HLGQLPAASAAGVLMDLGVSSPQIDNPVRGFSFRSEGPLDMRMDTTRGQSVAEWLADASIETMAEVIREYGEERFAQQVA
KAIDRRRQERGPLRTTAELAEVVAGAVKTREPGKDPATRTFQALRIFINAELEELQQALEASLKVLQPGGRLVVISFHSL
EDRIVKQFIAGQSREVFDRRAPFAAPKAMRLKALGRIKPSEEEIAANPRSRSAVMRVAERTGVSA
>Mature_305_residues
MQDTWTHRTVLLNEAIAALHINPDGHYIDATFGRGGHSRLLLSQLSPQGRLTAFDKDLDAIAEAQTITDPRFSIRHEGFM
HLGQLPAASAAGVLMDLGVSSPQIDNPVRGFSFRSEGPLDMRMDTTRGQSVAEWLADASIETMAEVIREYGEERFAQQVA
KAIDRRRQERGPLRTTAELAEVVAGAVKTREPGKDPATRTFQALRIFINAELEELQQALEASLKVLQPGGRLVVISFHSL
EDRIVKQFIAGQSREVFDRRAPFAAPKAMRLKALGRIKPSEEEIAANPRSRSAVMRVAERTGVSA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=37.6470588235294, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=36.1809045226131, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1786270, Length=315, Percent_Identity=55.8730158730159, Blast_Score=331, Evalue=3e-92,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=35.7142857142857, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=35.7142857142857, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_POLSJ (Q12EM3)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547917.1
- ProteinModelPortal:   Q12EM3
- SMR:   Q12EM3
- STRING:   Q12EM3
- GeneID:   4012054
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1067
- NMPDR:   fig|296591.1.peg.3338
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q12EM3
- ProtClustDB:   PRK00050
- BioCyc:   PSP296591:BPRO_1067-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33659; Mature: 33659

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 79-79 BINDING 96-96 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDTWTHRTVLLNEAIAALHINPDGHYIDATFGRGGHSRLLLSQLSPQGRLTAFDKDLDA
CCCCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCCHHHHHHHCCCCCCEEEHHHHHHH
IAEAQTITDPRFSIRHEGFMHLGQLPAASAAGVLMDLGVSSPQIDNPVRGFSFRSEGPLD
HHHHHHCCCCCHHHHHCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
MRMDTTRGQSVAEWLADASIETMAEVIREYGEERFAQQVAKAIDRRRQERGPLRTTAELA
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EVVAGAVKTREPGKDPATRTFQALRIFINAELEELQQALEASLKVLQPGGRLVVISFHSL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHH
EDRIVKQFIAGQSREVFDRRAPFAAPKAMRLKALGRIKPSEEEIAANPRSRSAVMRVAER
HHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHH
TGVSA
CCCCC
>Mature Secondary Structure
MQDTWTHRTVLLNEAIAALHINPDGHYIDATFGRGGHSRLLLSQLSPQGRLTAFDKDLDA
CCCCCCHHHHHHHCCEEEEEECCCCCEEEECCCCCCCHHHHHHHCCCCCCEEEHHHHHHH
IAEAQTITDPRFSIRHEGFMHLGQLPAASAAGVLMDLGVSSPQIDNPVRGFSFRSEGPLD
HHHHHHCCCCCHHHHHCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
MRMDTTRGQSVAEWLADASIETMAEVIREYGEERFAQQVAKAIDRRRQERGPLRTTAELA
EEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EVVAGAVKTREPGKDPATRTFQALRIFINAELEELQQALEASLKVLQPGGRLVVISFHSL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHH
EDRIVKQFIAGQSREVFDRRAPFAAPKAMRLKALGRIKPSEEEIAANPRSRSAVMRVAER
HHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHH
TGVSA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA