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The map label for this gene is rlmI [H]

Identifier: 91786960

GI number: 91786960

Start: 1090485

End: 1091753

Strand: Direct

Name: rlmI [H]

Synonym: Bpro_1062

Alternate gene names: 91786960

Gene position: 1090485-1091753 (Clockwise)

Preceding gene: 91786958

Following gene: 91786961

Centisome position: 20.97

GC content: 62.49

Gene sequence:

>1269_bases
ATGAAAATAATTCGACTGAAGGAAGGCAAAGAGCGCTCCTTGCTGCGCCGCCACCCCTGGATTTTTGACGGCGCCATCGC
CAAAGGGGGTGGGGATGCGGGTGAAACGGTCAGGGTGGAGAGCCATGCGGGGCAGTTCCTGGGCTGGGCTGCTTTCAGTC
CCAGCTCCAAAATTCGGGCGCGGGTCTGGAGCTTTGATGAAAGCCAGCGCATAGATGCTTCTTTTTTTGTAGCGCGTATT
GACCAAGCCATCAAAGCCAGGGACTTGTTCGACATTCAGAGTGATGGCCGACGCCTGGTCCACGGGGAGTCTGATGGCTT
GCCGGGCCTGGTGGTGGACCGTTATGCCGACACGCTGGTGGCGCAATTCTCCAGTTGCGGCACCGAACGCTGGAAAAGCG
TGATCGTGGAGGCCCTGCTGGCGCAAACCGGCCTGACCCGCCTGTATGAGCGGTCGGATGCCAGTGTGCGGTCGCTGGAA
GGTCTGCCGGAGGCAACGGGCTGGCTGAGTGGAGCCCCCACGCTTGTCGCTTCGCGTACTACGCTGCCCCCCGAGGGGGC
TAATTTTCCTTGGGGCGGCCCGGCGGAAAATTGCAGCGGCACGGAAATCATCATTACCGAACACGACTGGAAACTGAGCC
TGGACGTGGCGCAGGGCCACAAGACCGGCTTTTATCTGGACCAGCGCGACAGCCGGCAGAGGTTTGCCGCCTACACGCGG
CGCCGGCAGTTTGAGCGGGTCCTCAACTGCTACTGCTACACCGGCGGCTTTACGGTAGCCGCGCTGGCTGGCGGCGCGAA
GCACGTGACATCCATTGATTCGTCCGGACCCGCCCTGGACCGCGCCAAAGCCAATGTGGCCCTGAACGGTTTCGATGCCA
GCCGCACGACGATGCTGGATGCCGATGTGAACGCCATGCTGCGCCAGTACCAGAAAGAGGGCCGAACCTTCGACGCCATC
GTGCTCGACCCACCGAAGTTCGCGCCCACGGTCACCCATGCCGAGCGTGCGGCTCGGGCTTACAAGGACATCAACCGGCT
GGCGCTGGCCATCCTGGAGCCTGGCGGCGTGCTGTTCACTTACTCCTGCTCCGGCGGCATCAGCGCCGACCTGTTTCACA
AGATCGTCGCCTCGGCCGGCACCGATGCGGGTGTGGATGCCTTTATTTCCGAACGTATGGGCGGCGCGCCCGATCACCCC
ATGACGGTGGCGTTTCCGGAGGGTGAATACCTCAAGGGGCTGGTGCTGGTCAAAAAACCGGGTGCGTAG

Upstream 100 bases:

>100_bases
GGGGGGAAAGTGCTGAAATTGAGTGCAGCGGTCGCTTTGGGCCAGCGGGCAGCCGGTTTGGGGCCGCAGGGGTCCAGATT
CAAACCACGACAATAGCGGC

Downstream 100 bases:

>100_bases
CCGTTTGCGCGGTCCGGTGATGGATCAAACCGGCTTGCGGCCCTTGTGACAGGATGCAATTTGCTACTAATATAGTAGCA
AATTGCAATGCAGGGGAGCC

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKIIRLKEGKERSLLRRHPWIFDGAIAKGGGDAGETVRVESHAGQFLGWAAFSPSSKIRARVWSFDESQRIDASFFVARI
DQAIKARDLFDIQSDGRRLVHGESDGLPGLVVDRYADTLVAQFSSCGTERWKSVIVEALLAQTGLTRLYERSDASVRSLE
GLPEATGWLSGAPTLVASRTTLPPEGANFPWGGPAENCSGTEIIITEHDWKLSLDVAQGHKTGFYLDQRDSRQRFAAYTR
RRQFERVLNCYCYTGGFTVAALAGGAKHVTSIDSSGPALDRAKANVALNGFDASRTTMLDADVNAMLRQYQKEGRTFDAI
VLDPPKFAPTVTHAERAARAYKDINRLALAILEPGGVLFTYSCSGGISADLFHKIVASAGTDAGVDAFISERMGGAPDHP
MTVAFPEGEYLKGLVLVKKPGA

Sequences:

>Translated_422_residues
MKIIRLKEGKERSLLRRHPWIFDGAIAKGGGDAGETVRVESHAGQFLGWAAFSPSSKIRARVWSFDESQRIDASFFVARI
DQAIKARDLFDIQSDGRRLVHGESDGLPGLVVDRYADTLVAQFSSCGTERWKSVIVEALLAQTGLTRLYERSDASVRSLE
GLPEATGWLSGAPTLVASRTTLPPEGANFPWGGPAENCSGTEIIITEHDWKLSLDVAQGHKTGFYLDQRDSRQRFAAYTR
RRQFERVLNCYCYTGGFTVAALAGGAKHVTSIDSSGPALDRAKANVALNGFDASRTTMLDADVNAMLRQYQKEGRTFDAI
VLDPPKFAPTVTHAERAARAYKDINRLALAILEPGGVLFTYSCSGGISADLFHKIVASAGTDAGVDAFISERMGGAPDHP
MTVAFPEGEYLKGLVLVKKPGA
>Mature_422_residues
MKIIRLKEGKERSLLRRHPWIFDGAIAKGGGDAGETVRVESHAGQFLGWAAFSPSSKIRARVWSFDESQRIDASFFVARI
DQAIKARDLFDIQSDGRRLVHGESDGLPGLVVDRYADTLVAQFSSCGTERWKSVIVEALLAQTGLTRLYERSDASVRSLE
GLPEATGWLSGAPTLVASRTTLPPEGANFPWGGPAENCSGTEIIITEHDWKLSLDVAQGHKTGFYLDQRDSRQRFAAYTR
RRQFERVLNCYCYTGGFTVAALAGGAKHVTSIDSSGPALDRAKANVALNGFDASRTTMLDADVNAMLRQYQKEGRTFDAI
VLDPPKFAPTVTHAERAARAYKDINRLALAILEPGGVLFTYSCSGGISADLFHKIVASAGTDAGVDAFISERMGGAPDHP
MTVAFPEGEYLKGLVLVKKPGA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=414, Percent_Identity=45.8937198067633, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1787181, Length=270, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45829; Mature: 45829

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIRLKEGKERSLLRRHPWIFDGAIAKGGGDAGETVRVESHAGQFLGWAAFSPSSKIRA
CEEEEECCCCHHHHHHHCCCEEECCEECCCCCCCCEEEEECCCCCEEEEEECCCCCHHEE
RVWSFDESQRIDASFFVARIDQAIKARDLFDIQSDGRRLVHGESDGLPGLVVDRYADTLV
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHH
AQFSSCGTERWKSVIVEALLAQTGLTRLYERSDASVRSLEGLPEATGWLSGAPTLVASRT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHCCCCCCCCEEEECC
TLPPEGANFPWGGPAENCSGTEIIITEHDWKLSLDVAQGHKTGFYLDQRDSRQRFAAYTR
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCHHHHHHHHHH
RRQFERVLNCYCYTGGFTVAALAGGAKHVTSIDSSGPALDRAKANVALNGFDASRTTMLD
HHHHHHHHHEEEECCCCEEEECCCCCHHHEECCCCCCCCHHCCCEEEEECCCCCCEEEEC
ADVNAMLRQYQKEGRTFDAIVLDPPKFAPTVTHAERAARAYKDINRLALAILEPGGVLFT
CCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEE
YSCSGGISADLFHKIVASAGTDAGVDAFISERMGGAPDHPMTVAFPEGEYLKGLVLVKKP
EECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECC
GA
CC
>Mature Secondary Structure
MKIIRLKEGKERSLLRRHPWIFDGAIAKGGGDAGETVRVESHAGQFLGWAAFSPSSKIRA
CEEEEECCCCHHHHHHHCCCEEECCEECCCCCCCCEEEEECCCCCEEEEEECCCCCHHEE
RVWSFDESQRIDASFFVARIDQAIKARDLFDIQSDGRRLVHGESDGLPGLVVDRYADTLV
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHH
AQFSSCGTERWKSVIVEALLAQTGLTRLYERSDASVRSLEGLPEATGWLSGAPTLVASRT
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHCCCCCCCCEEEECC
TLPPEGANFPWGGPAENCSGTEIIITEHDWKLSLDVAQGHKTGFYLDQRDSRQRFAAYTR
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCEEECCCCHHHHHHHHHH
RRQFERVLNCYCYTGGFTVAALAGGAKHVTSIDSSGPALDRAKANVALNGFDASRTTMLD
HHHHHHHHHEEEECCCCEEEECCCCCHHHEECCCCCCCCHHCCCEEEEECCCCCCEEEEC
ADVNAMLRQYQKEGRTFDAIVLDPPKFAPTVTHAERAARAYKDINRLALAILEPGGVLFT
CCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCEEEE
YSCSGGISADLFHKIVASAGTDAGVDAFISERMGGAPDHPMTVAFPEGEYLKGLVLVKKP
EECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEEECC
GA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA