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The map label for this gene is pilD [H]

Identifier: 91786745

GI number: 91786745

Start: 866359

End: 867216

Strand: Direct

Name: pilD [H]

Synonym: Bpro_0843

Alternate gene names: 91786745

Gene position: 866359-867216 (Clockwise)

Preceding gene: 91786744

Following gene: 91786746

Centisome position: 16.66

GC content: 60.14

Gene sequence:

>858_bases
ATGCCGACGGGATTGGGTTTGGGGCCGGAGTTTGAGGCGCTGCTGGCCGGATTTTTTGGCCTGCTGATCGGCAGCTTCCT
GAACGTCGTCATCTACCGCCTGCCCAAAATGATGGAGCGGCAGTGGGCGCAGGAGTGCGCCGACCTGGCCGGAAAATCCG
TGGAGCCCGGAGAAAAATTCAATTTGGTGGCACCGCGGTCGCGCTGCTCCAATTGCGGCCATGTCATTCGCTGGTACGAG
AACATCCCGGTGGCGAGCTACCTGTTCCTCCGCGGGAAATGCTCCATGTGCAGCGCCCCCTACGGCGTGCGCTATCCCAT
CGTGGAGGCGATCACCGGCGCCCTATTTTTCTTTTGCGCATGGCGTTGGGGCTGGTCGCTCGCTGCGCTGGTCTGGTGCG
GATTTTCGGCAGCGCTGCTGGTGCTGGCACTGATTGATTGGGACACGACCTTGCTGCCGGACGACATCACCTTGCCCTTG
CTGTGGGCTGGATTGATCGTGGCGGCGCTGCAGTGGAATCCGGCCGTGAATCTGTACAGTGCCCTGTGGGGTGCGATTGC
CGGTTATCTCTCGCTCTGGCTGGTGTACTGGGCCTTCAAACTGGTGACCGGAAAAGAAGGCATGGGTTACGGCGACTTCA
AACTGTTTGCCGCACTGGGTGCCTGGTTTGGCTGGCAGGCGCTGATTCCGATGATCCTGATGGCATCCATCATTGGCGCC
ATCATTGGCATTGCGCTGAAGTTTTCCAGCGGACTGCGCGAGGGCGGTTACGTGCCGTTTGGCCCTTTCCTGGCGGGTGC
GGGATTTACCGCCATGGTGTTCGGGCCTCAATCGATTCTGCAGTTTGTGGGTTTGTGA

Upstream 100 bases:

>100_bases
GCCTGATGGAGCCTATCATCATCGTGTTTCTGGGCACGTTGATTGGCGGTATCGTGGTTTCCATGTACCTGCCCATTTTC
AAGCTGGGTCAAGTCGTTTA

Downstream 100 bases:

>100_bases
TGCCATGCCCTTCGCAGCCCAGCGTATCGGACTGACTGGCGGCATAGGCAGCGGCAAGAGTACGGTGGCCCGCCTGCTGG
TGGGACGGGGCGCAACGCTG

Product: type 4 prepilin peptidase 1

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPTGLGLGPEFEALLAGFFGLLIGSFLNVVIYRLPKMMERQWAQECADLAGKSVEPGEKFNLVAPRSRCSNCGHVIRWYE
NIPVASYLFLRGKCSMCSAPYGVRYPIVEAITGALFFFCAWRWGWSLAALVWCGFSAALLVLALIDWDTTLLPDDITLPL
LWAGLIVAALQWNPAVNLYSALWGAIAGYLSLWLVYWAFKLVTGKEGMGYGDFKLFAALGAWFGWQALIPMILMASIIGA
IIGIALKFSSGLREGGYVPFGPFLAGAGFTAMVFGPQSILQFVGL

Sequences:

>Translated_285_residues
MPTGLGLGPEFEALLAGFFGLLIGSFLNVVIYRLPKMMERQWAQECADLAGKSVEPGEKFNLVAPRSRCSNCGHVIRWYE
NIPVASYLFLRGKCSMCSAPYGVRYPIVEAITGALFFFCAWRWGWSLAALVWCGFSAALLVLALIDWDTTLLPDDITLPL
LWAGLIVAALQWNPAVNLYSALWGAIAGYLSLWLVYWAFKLVTGKEGMGYGDFKLFAALGAWFGWQALIPMILMASIIGA
IIGIALKFSSGLREGGYVPFGPFLAGAGFTAMVFGPQSILQFVGL
>Mature_284_residues
PTGLGLGPEFEALLAGFFGLLIGSFLNVVIYRLPKMMERQWAQECADLAGKSVEPGEKFNLVAPRSRCSNCGHVIRWYEN
IPVASYLFLRGKCSMCSAPYGVRYPIVEAITGALFFFCAWRWGWSLAALVWCGFSAALLVLALIDWDTTLLPDDITLPLL
WAGLIVAALQWNPAVNLYSALWGAIAGYLSLWLVYWAFKLVTGKEGMGYGDFKLFAALGAWFGWQALIPMILMASIIGAI
IGIALKFSSGLREGGYVPFGPFLAGAGFTAMVFGPQSILQFVGL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=267, Percent_Identity=38.2022471910112, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1789732, Length=164, Percent_Identity=45.7317073170732, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31107; Mature: 30976

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEECHHHHHHHHCC
>Mature Secondary Structure 
PTGLGLGPEFEALLAGFFGLLIGSFLNVVIYRLPKMMERQWAQECADLAGKSVEPGEKF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NLVAPRSRCSNCGHVIRWYENIPVASYLFLRGKCSMCSAPYGVRYPIVEAITGALFFFCA
CEECCHHHHHCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
WRWGWSLAALVWCGFSAALLVLALIDWDTTLLPDDITLPLLWAGLIVAALQWNPAVNLYS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
ALWGAIAGYLSLWLVYWAFKLVTGKEGMGYGDFKLFAALGAWFGWQALIPMILMASIIGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGIALKFSSGLREGGYVPFGPFLAGAGFTAMVFGPQSILQFVGL
HHHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]