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The map label for this gene is dsbA [C]

Identifier: 91212466

GI number: 91212466

Start: 3409619

End: 3410287

Strand: Direct

Name: dsbA [C]

Synonym: UTI89_C3474

Alternate gene names: 91212466

Gene position: 3409619-3410287 (Clockwise)

Preceding gene: 91212465

Following gene: 91212467

Centisome position: 67.31

GC content: 44.69

Gene sequence:

>669_bases
ATGTCGAAATTAGGGATCTCATCACTATTTAAAACCATACTGCTTACCGCAGCTCTGGCGGTTTCATTTACCGCTTCTGC
ATTTACCGAAGGCACGGATTACATGGTTCTGGAAAAACCAATTCCGAATGCCGACAAAACGCTGATTAAGGTATTTAGCT
ACGCCTGCCCGTTCTGTTACAAGTACGACAAAGCGGTTACCGGTCCGGTATCGGAAAAAGTGAAAGATATCGTTGCTTTC
ACGCCATTCCATCTGGAAACAAAAGGTGAATACGGCAAACAAGCAAGCGAAGTCTTTGCGGTCTTGATCAATAAAGATAA
AGCAGCAGGCATTTCCTTATTTGATGCTAACTCTCAGTTTAAGAAAGCCAAGTTTGCCTACTACGCGGCTTATCACGACA
AAAAAGAACGCTGGTCTGACGGTAAAGATCCGGCTGCTTTTATCAAAACCGGTCTGGATGCCGCAGGTATGAGTCAGGCG
GATTTTGAAGCGGCACTGAAAGAACCAGCGGTTCAAGAAACACTGAAAAAATGGAAAGCCTCTTATGATGTCGCCAAAAT
CCAGGGAGTTCCAGCCTATGTCGTTAATGGTAAGTACTTGATCTACACCAAGAGCATCAAATCCATCGACGCGATGGCAG
ACCTTATCCGTGAGTTAGCAAGCAAATAA

Upstream 100 bases:

>100_bases
TGGAAATCGACGTGCTGTCAGATAAACCCAATCAGACTCACTACCGTGCGCTGTTAGTCCGTCCACAACAGATGTTCAAA
TAATTAAGAAGGGAATAAAA

Downstream 100 bases:

>100_bases
CAGGGAAACAGGCTATGGGTATTAAGGGAATGTGGAAAGATCTTCGCACATCACCAGTTGACACACTGGTCAGATGGCAG
GAGCAACGCTTGTTGTGGTT

Product: putative disulfide isomerase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAF
TPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
DFEAALKEPAVQETLKKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK

Sequences:

>Translated_222_residues
MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAF
TPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
DFEAALKEPAVQETLKKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK
>Mature_221_residues
SKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFT
PFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQAD
FEAALKEPAVQETLKKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=227, Percent_Identity=28.6343612334802, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24380; Mature: 24249

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHH
KYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQF
HCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHH
KKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQADFEAALKEPAVQETLKKWKA
HHHHHHHHHHHHCHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
SYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK
CCCHHHHCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNADKTLIKVFSYACPFCY
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHH
KYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVLINKDKAAGISLFDANSQF
HCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHH
KKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQADFEAALKEPAVQETLKKWKA
HHHHHHHHHHHHCHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
SYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRELASK
CCCHHHHCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]