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The map label for this gene is ygfA [H]

Identifier: 91212291

GI number: 91212291

Start: 3233688

End: 3234434

Strand: Direct

Name: ygfA [H]

Synonym: UTI89_C3298

Alternate gene names: 91212291

Gene position: 3233688-3234434 (Clockwise)

Preceding gene: 91212290

Following gene: 162138380

Centisome position: 63.83

GC content: 52.07

Gene sequence:

>747_bases
ATGTGGCGCTCCGCGGTTGGTGAGCATGCTCGGTCCGTCCGAGAAGCCTTAAAACTGCGACGACACATTCACCTTGAACC
AAGGGTTCAAGGGTTACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTACCTGGCACCAGCCATGACGCAACTACCAG
AACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTACGTTAACGCCGGAACAACAG
CAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACGTATCCCCCAGTGGTGATGGCGCATACGGTCGCTGTATTCCT
CTCTTTTGATGGCGAACTCGACACCCAGCCACTCATAGAACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTT
TGCATCCCTTTAGTGCCGGTAACTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATC
CACGAGCCAAAACTGGACGTGCGCGATGTGCTTCCCCTTTCCCGGTTAGATGTATTGATCACGCCGCTGGTGGCTTTTGA
TGAATACGGTCAACGCTTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAATGCAAC
CGGTGGGTTATGCGCATGATTGCCAGTTAGTAGAAAAACTCCCAGTGGAGAAATGGGATATTCCACTGCCTGCGGTGGTT
ACACCGTCGAAAGTCTGGGAATGGTAA

Upstream 100 bases:

>100_bases
ACTTTTCGGTTTACTGTGGTAGAGTAACCGTGAAGACAAAATTTCTCTGAGATGTTCGCAAGCGGGCCAGTCCCCTGAGC
CGATATTTCATACCACAAGA

Downstream 100 bases:

>100_bases
GGGCGATACATCCCGCATCGCCCTGATTGACATCGTTGATTCTTTGACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTG
TTCCTCCCCTGTGACTGACT

Product: putative ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MWRSAVGEHARSVREALKLRRHIHLEPRVQGLQPAAASRRFPSYLAPAMTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQ
QEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKI
HEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVV
TPSKVWEW

Sequences:

>Translated_248_residues
MWRSAVGEHARSVREALKLRRHIHLEPRVQGLQPAAASRRFPSYLAPAMTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQ
QEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKI
HEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVV
TPSKVWEW
>Mature_248_residues
MWRSAVGEHARSVREALKLRRHIHLEPRVQGLQPAAASRRFPSYLAPAMTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQ
QEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKI
HEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVV
TPSKVWEW

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Homo sapiens, GI5453746, Length=192, Percent_Identity=23.4375, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789278, Length=182, Percent_Identity=98.3516483516484, Blast_Score=373, Evalue=1e-105,
Organism=Drosophila melanogaster, GI161077430, Length=189, Percent_Identity=24.3386243386243, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: NA

Molecular weight: Translated: 28757; Mature: 28757

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRSAVGEHARSVREALKLRRHIHLEPRVQGLQPAAASRRFPSYLAPAMTQLPELPLTLS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCC
RQEIRKMIRQRRRTLTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHH
QLWRAGKRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLI
HHHHCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHH
TPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
TPSKVWEW
CCHHHCCC
>Mature Secondary Structure
MWRSAVGEHARSVREALKLRRHIHLEPRVQGLQPAAASRRFPSYLAPAMTQLPELPLTLS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCC
RQEIRKMIRQRRRTLTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHH
QLWRAGKRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLI
HHHHCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHH
TPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKMQPVGYAHDCQLVEKLPVEKWDIPLPAVV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEE
TPSKVWEW
CCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]