Definition Escherichia coli UTI89 plasmid pUTI89, complete sequence.
Accession NC_007941
Length 114,230

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The map label for this gene is traN [H]

Identifier: 91206368

GI number: 91206368

Start: 91590

End: 93440

Strand: Direct

Name: traN [H]

Synonym: UTI89_P123

Alternate gene names: 91206368

Gene position: 91590-93440 (Clockwise)

Preceding gene: 91206367

Following gene: 91206369

Centisome position: 80.18

GC content: 53.16

Gene sequence:

>1851_bases
ATGAAACGTATTTTACCTCTGATACTGGCTCTGGTTGCCGGCATGGCACAGGCTGACAGCAACAGTGATTACCGGGCCGG
TTCTGATTTTGCCCGTCAGATTCAGGGGCAGGGTACAGGCAGTATTCAGGGCTTTAAGCCACAGGAGAGTATCCCCAGCT
ATAACGCGAATCCGGACGAGACAAAATATTACGGTGGCGTGACTGCCGGCGGGGATGGCGGCCTGAAAAATGACGGCACC
ACGGAATGGGCGACCGGTGAAACCGGAAAAACCATCACAGAGTCCTTCATGAACAAGCCGAAAGACATTCTGTCACCGGA
TGCACCGTTCATTCAGACCGGCAGGGATGTGGTGAACCGGGCTGACAGCATTGTGGGAAACACCGGGCAGCAGTGCAGTG
CGCAGCAGATAAACCGCAGTGAGTTCACAAACTATACCTGTGAGCGGGACACGATGGTGGAGGAGTACTGCACACGGACT
GCCAGTATCACCGGGGACTGGAAATACACGGATGAATATCGTGAGGTGACGATACCGCACTCACAGTTCCGGTTCAGTAT
GAACGGTCTGAAACTGGTGTTCAGTGTCACGGCTCCGGTAACCGGTACAGTGGAAAGTGCATCATTGTCTGTATACGCCG
CTTTTTTCTTCCTGAACTCCCGTTATACCTTCATGAATACAACCTTTAATGTCGGCCTGGCAAACGGGCAGTCGGATACT
TACCCCCTGTCCGGGGCGACAGGTTTGCAGGTGACACAGGGGCAGGTTCTGACCGGTAGTGGCTGTACTGCTAACGGTAA
TTGCCTCCCGCACGGGAATGGTGACAGGAAAGTCTATGAATCGCTTGTCAGTGGTGCTTCCACCTTTACGCTGAAACTCA
GGATGAAGGTCCGTGATAAAGAATGGGTTCCCCGGGTGGAGTGGGTGGAGAGTTGTCCGTTTAATAAAGCGGATGGCGTG
CTGACAGGCACTGAGTGTTCAGAGCCGGGTGGTACGAAAACCGGAGTGATGGAAGGTAAACCCTGGAACATCACACAGGC
ATGCTGGGCATACCGGGATAAATATGTCACTCAGTCCGCAGATAACGGCACCTGTCAGAAATATGTCGATAATCCGGCCT
GTACGCTGGCCTCACGTCAGTGCGCGTTTTATTCCGATGAGGGAACCTGTCTGCATGAGTACGCCACGTACTCCTGCGAG
TCAAAAACATCCGGGAAAGTGATGGTCTGTGGGGGAGACGTCTTCTGTCTCGATGGCGAATGTGATAAGGCTCAGAGCGG
GCAGAGTAATGATTTTGCCGAAGCCGTTTCTCAGCTGGCGGCACTGGCCGCAGCAGGTAAGGATGTTGCGGCGCTGAACG
GGGTGGATGTCCGTGCTTTCACCGGTCAGGCGAAATTTTGTAAGAAGGCTGCAGCCGGATACAGCAACTGCTGTAAGGAC
AGCGGCTGGGGACAGGATATCGGGCTGGCCAAATGCAGCAGTGATGAAAAGGCCCTGGCTAAAGCAAAATCAAACAAACT
GACCGTCAGTGTCGGAGAGTTCTGTTCGAAGAAAGTGCTGGGTGTCTGTCTGCAGAAAAAACGCAGCTACTGTCAGTTTG
ATTCGAAGCTGGCGCAGATAGTCCAGCAGCAGGGGCGTAACGGGCAGTTGCGTATCGGGTTTGGCAGTGCGAAGCATCCT
GACTGTCGGGGGATTACGGTTGATGAGCTGCAGAAAATTCAGTTCGACAGACTGGATTTCACTAACTTCTACGAAGACCT
GATGAATAACCAGAAAATCCCGGACAGTGGCGTTCTGACGCAGAAAGTGAAAGAGCAGATTGCTGACCAGCTGAAACAGG
CAGGACAGTAA

Upstream 100 bases:

>100_bases
GAAACCTGCGGGTCGGCCAGGCGCTGGAGAAAGTGGCCGCCACGGGTGACTGCCGGCAGGTGGCTCATGATTTACTGGCA
GGGAAAGGAGATTCCGGGAA

Downstream 100 bases:

>100_bases
GGTATGGAAAATAACCGGGGGAAATGATGAAAGTCATATTCACGTCGAACCGTTTTATTGATTTTCTTATCCGGCTTTTG
ATTACAGCGATTGTCATTTC

Product: conjugal transfer mating pair stabilization protein TraN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQGFKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGT
TEWATGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCTRT
ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDT
YPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGV
LTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSCE
SKTSGKVMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAAAGYSNCCKD
SGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHP
DCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ

Sequences:

>Translated_616_residues
MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQGFKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGT
TEWATGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCTRT
ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDT
YPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGV
LTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSCE
SKTSGKVMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAAAGYSNCCKD
SGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHP
DCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ
>Mature_616_residues
MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQGFKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGT
TEWATGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCTRT
ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDT
YPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGV
LTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSCE
SKTSGKVMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAAAGYSNCCKD
SGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHP
DCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ

Specific function: Essential for F plasmid conjugative transfer. May interact with the recipient cell surface to stabilize mating pairs initiated by F-pili. May interact with traG [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009726
- InterPro:   IPR014121 [H]

Pfam domain/function: PF06986 TraN [H]

EC number: NA

Molecular weight: Translated: 66724; Mature: 66724

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQGFKPQESIPSYNANPDE
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCC
TKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNR
CEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCHHHCCHHHHHH
ADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPH
HHHHHCCCCCCCCHHHHCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCEEECCH
SQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDT
HHHEEECCCEEEEEEECCCCCCCCCCCHHHHEEEEEHHCCCEEEEEEEEEEEEECCCCCC
YPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDK
CCCCCCCCCEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCC
EWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSA
CCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
DNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSCESKTSGKVMVCGGDVFCLDGE
CCCCHHHHCCCCHHHHHCCCEEEECCCCCEEHHHHHCCCCCCCCCEEEEECCCEEEECCC
CDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAAAGYSNCCKD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCEEEEECCHHHHHHHHHCCHHHHHCC
SGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQI
CCCCCCCCCEECCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQQQGRNGQLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLT
HHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHH
QKVKEQIADQLKQAGQ
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQGFKPQESIPSYNANPDE
CHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCC
TKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNR
CEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHCCCCCCHHHCCHHHHHH
ADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPH
HHHHHCCCCCCCCHHHHCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCEEECCH
SQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDT
HHHEEECCCEEEEEEECCCCCCCCCCCHHHHEEEEEHHCCCEEEEEEEEEEEEECCCCCC
YPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDK
CCCCCCCCCEEECCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEECCC
EWVPRVEWVESCPFNKADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSA
CCCCHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
DNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSCESKTSGKVMVCGGDVFCLDGE
CCCCHHHHCCCCHHHHHCCCEEEECCCCCEEHHHHHCCCCCCCCCEEEEECCCEEEECCC
CDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAAAGYSNCCKD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCEEEEECCHHHHHHHHHCCHHHHHCC
SGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQI
CCCCCCCCCEECCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQQQGRNGQLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLT
HHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHH
QKVKEQIADQLKQAGQ
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1593622; 7915817; 2050638 [H]