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The map label for this gene is recE [H]

Identifier: 89885798

GI number: 89885798

Start: 76220

End: 77245

Strand: Reverse

Name: recE [H]

Synonym: Rfer_4312

Alternate gene names: 89885798

Gene position: 77245-76220 (Counterclockwise)

Preceding gene: 89885808

Following gene: 89885786

Centisome position: 30.0

GC content: 55.75

Gene sequence:

>1026_bases
ATGACAAACGATCAATTCAGAAACAGGAACACCCCGAGACTTACTCTCGTCAGTGGCGGCGGCATTCCACTAGCGACTCC
CCTGCCAGGCCTCCGGCTTCCAGCCTACGCGCTGGACGGTCGGAAATTGACTTGGACTGCGGACCGTACGGCCGTGTGGT
TTGATAGCACTGATGAGGAATACCACAGCGACGATTTCGCTGCGAGTTCAAGTGCACTCAAGCTCATGGACCGGTCGGCG
GCGCATCTGATGGCCCAGCGCCAGCATCGCGCGCAATTGCGGGCAGCGGGTAAGGACGAACCCAACCGGGCCTTTATGTT
CGGAACCGCCGTTCACTGCGCCGTACTGGAGCCCCACATCTTCAAGACCAGGTACGTGGTCTATCCGTGCGTGGAAAGGC
AAGGGGTTCGCTTGACCAAAGCCTACAAGACTTTCGAGCAGACCAACCCGAAAAAGCGCATTCTCCTGCCCTCGGAAATG
GCGAGTATTCAAGACTGCGCCAGACGGGTGCTGGCGACCTCCGTCATCCGCACTGACAGCGAGTCGTTCACCATGGCCGA
CCTGGTGGATCTGGGGGCCACTGAACGAAATTATTACTGGGTCGATCTGCTGACTGGGGTAACTTGCAAGGCCAGGATGG
ACCTGACGGTGGAAAACATCATCCTGGATATCAAAACCGCCATCGATGCGCGCTATGACCGCTTCAAGTACGACGCAGGG
AAGTTCGGTTATCACATTCAAGCTGCCTTTTACATGGAAGGCTATTCCCGCTTCGTGCCAGAAGGGCGCAATATCATCAT
GACTTTTCTGGTCGCCGAAAAAGAGAGTCCTCACGCCACCATCGTTTACGAGGCTGACAAAGACACGTTTTGGGGCTTTG
GCAGCAAGCGGGTGCGCGAGTTGCTGGCACAGTACAAACAATGCCTGCAAACGCAGCATTGGCCCGCGTACGCCGAAGGG
GCTCAATTGCTCAAGCTGCCCATGAATAAGCTTTACCAAGAGCCAAAATACAATTTCAGCTACTGA

Upstream 100 bases:

>100_bases
CAGAATTACATAAAAGCTCTGGCTGTCAAGTTATCGAGGAGGCGGATTCGAGGACCGAAAAATAGCTTGCCGAAACAGGC
CGCTCATGTAATCTGAACCT

Downstream 100 bases:

>100_bases
CCGTGCTTAAATATTCAGCACATCAGAAGTATCAAGCTTCGGTGCGGGTTTCGGGTCAAAGACAGGTCAGGTTATCCAGT
CAAATTGTGGATAACCTCGG

Product: hypothetical protein

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTNDQFRNRNTPRLTLVSGGGIPLATPLPGLRLPAYALDGRKLTWTADRTAVWFDSTDEEYHSDDFAASSSALKLMDRSA
AHLMAQRQHRAQLRAAGKDEPNRAFMFGTAVHCAVLEPHIFKTRYVVYPCVERQGVRLTKAYKTFEQTNPKKRILLPSEM
ASIQDCARRVLATSVIRTDSESFTMADLVDLGATERNYYWVDLLTGVTCKARMDLTVENIILDIKTAIDARYDRFKYDAG
KFGYHIQAAFYMEGYSRFVPEGRNIIMTFLVAEKESPHATIVYEADKDTFWGFGSKRVRELLAQYKQCLQTQHWPAYAEG
AQLLKLPMNKLYQEPKYNFSY

Sequences:

>Translated_341_residues
MTNDQFRNRNTPRLTLVSGGGIPLATPLPGLRLPAYALDGRKLTWTADRTAVWFDSTDEEYHSDDFAASSSALKLMDRSA
AHLMAQRQHRAQLRAAGKDEPNRAFMFGTAVHCAVLEPHIFKTRYVVYPCVERQGVRLTKAYKTFEQTNPKKRILLPSEM
ASIQDCARRVLATSVIRTDSESFTMADLVDLGATERNYYWVDLLTGVTCKARMDLTVENIILDIKTAIDARYDRFKYDAG
KFGYHIQAAFYMEGYSRFVPEGRNIIMTFLVAEKESPHATIVYEADKDTFWGFGSKRVRELLAQYKQCLQTQHWPAYAEG
AQLLKLPMNKLYQEPKYNFSY
>Mature_340_residues
TNDQFRNRNTPRLTLVSGGGIPLATPLPGLRLPAYALDGRKLTWTADRTAVWFDSTDEEYHSDDFAASSSALKLMDRSAA
HLMAQRQHRAQLRAAGKDEPNRAFMFGTAVHCAVLEPHIFKTRYVVYPCVERQGVRLTKAYKTFEQTNPKKRILLPSEMA
SIQDCARRVLATSVIRTDSESFTMADLVDLGATERNYYWVDLLTGVTCKARMDLTVENIILDIKTAIDARYDRFKYDAGK
FGYHIQAAFYMEGYSRFVPEGRNIIMTFLVAEKESPHATIVYEADKDTFWGFGSKRVRELLAQYKQCLQTQHWPAYAEGA
QLLKLPMNKLYQEPKYNFSY

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=285, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 39008; Mature: 38877

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDQFRNRNTPRLTLVSGGGIPLATPLPGLRLPAYALDGRKLTWTADRTAVWFDSTDEE
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCEEEEECCCCCC
YHSDDFAASSSALKLMDRSAAHLMAQRQHRAQLRAAGKDEPNRAFMFGTAVHCAVLEPHI
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEECCCC
FKTRYVVYPCVERQGVRLTKAYKTFEQTNPKKRILLPSEMASIQDCARRVLATSVIRTDS
EEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCC
ESFTMADLVDLGATERNYYWVDLLTGVTCKARMDLTVENIILDIKTAIDARYDRFKYDAG
CCCCHHHHHHCCCCCCCEEEEEEECCCEEEEEECEEHHHHEEHHHHHHHHHHHHHCCCCC
KFGYHIQAAFYMEGYSRFVPEGRNIIMTFLVAEKESPHATIVYEADKDTFWGFGSKRVRE
CCCEEEEEEEEECHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHH
LLAQYKQCLQTQHWPAYAEGAQLLKLPMNKLYQEPKYNFSY
HHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TNDQFRNRNTPRLTLVSGGGIPLATPLPGLRLPAYALDGRKLTWTADRTAVWFDSTDEE
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCEEEEECCCEEEEECCCCCC
YHSDDFAASSSALKLMDRSAAHLMAQRQHRAQLRAAGKDEPNRAFMFGTAVHCAVLEPHI
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEEEEEECCCC
FKTRYVVYPCVERQGVRLTKAYKTFEQTNPKKRILLPSEMASIQDCARRVLATSVIRTDS
EEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCC
ESFTMADLVDLGATERNYYWVDLLTGVTCKARMDLTVENIILDIKTAIDARYDRFKYDAG
CCCCHHHHHHCCCCCCCEEEEEEECCCEEEEEECEEHHHHEEHHHHHHHHHHHHHCCCCC
KFGYHIQAAFYMEGYSRFVPEGRNIIMTFLVAEKESPHATIVYEADKDTFWGFGSKRVRE
CCCEEEEEEEEECHHHHHCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHH
LLAQYKQCLQTQHWPAYAEGAQLLKLPMNKLYQEPKYNFSY
HHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]