Click here to switch to the map view.

The map label for this gene is outE [H]

Identifier: 89885733

GI number: 89885733

Start: 1

End: 1791

Strand: Reverse

Name: outE [H]

Synonym: Rfer_4246

Alternate gene names: 89885733

Gene position: 1791-1 (Counterclockwise)

Preceding gene: 89885734

Following gene: NA

Centisome position: 0.7

GC content: 55.16

Gene sequence:

>1791_bases
ATGTCACTTTTAGATGTATTCAAGCGGACGTCAACTCGCGCTACGCTGGTGGCCCGCGGGCCCTCTGGTGGGGCATTACG
TGATCGTGCGGCGTCAGCGCCAGCATCGTTTGCAACGACGGGCCATGGTACGGGCCAGAAACAGGGGCAGTCTGCACGGG
TTGGGGTGATCCAGGACCAGGTGGATTTGCCTCTCTACAGGGAATTAGCTCCGATTCCGGCGAATGCCAAAGATTTCGCG
GCGCTCTTGGTATCCCTTGATGGAAGGACGGTCTATTTGGCTGTGCTGCCTGAGATGGCAACACGGGCCATCACACACAC
GATCAAGCAATCGTTGAGTCACAGCGGCTCCTACGACAACGTGAAGATGATCGAGGTGACCACAAACGTGTTGCACGCTC
TGCACGCCGCCAGATCTATCGGTCGCCTAGACACCCAGGAGGTCGCGGGCGAGGCGAAGGGTTTGAATATCCGCATGGGC
TTCGAGCTCATTGCAACTGGCATCGAAATGGAAGCCAGTGACATTCACATTGACATTCGCAATACTGGGCGCGATGCACC
GATGGTGTTGGTGCGCTTAAGAATCCACGGTGCGCTGGAGGAACATGCCTGTTTGCGTACCGCAGAGGCCGTCCAAGTCT
ATACAGAAATGGTTCGCGGCCTATATCAGAACGACACGATATCAAACGCGCTGGTGCGTACGAGCGGTACTTTCAACGAG
TTGGATCGGCAAGAGGCAATGCTGAAGCCTCCAATCAAGAACGCCGAGTTGCGTTTCGAATCGTTACCGGAAAAAGGCGG
CTTTGACGTTGTTATGCGCATTAACGGGCACGACGGCAAATCGGCAGCCCGCAAGAGCCTTGACCAACTTGGTTTGAGTG
ACGATCACACGCGAGACCTAACTCGCGCGTCGCAAGCGCCCCATGGATTGGTGGTAGTTGTGGGGTCAACGGGCTCGGGC
AAGACGACCACGATTACAACGATCTTGGCAATGGACCCCTCGGCCAAGTACAAAAAGCGGATTTCATTGGAAATTCCACC
CGAGTCGGACATCCCCTATCTATCGCAAGTCCCCGTCAAAGAGTCGGACCTGAACAACAAAATGGATGGTGTGATGCGTA
GCGATCCCGACGTGATATCTGCCGGCGAGATACGCAATCGTGAGACAGCACTTATGGCGCAGGATTCGTCAATTACAGGG
CATTTGACGTTCGCCACTTTTCACGCCAATGGAATTCTCTTTTCACTGCAACGCATGCTGTCAGAACGGATTGGATTTGA
CCCTCACATTCTGACGGCCGACGGCTTTTTGCGTGCGGTGTTGTACCAGACACTGGTGGAAATCTTGTGTCCCACATGCA
AGCAGCCCGCGCTCAATGCGCTCGCACCAGACAAGGTTGAACTGCTCAAGACCAAATTCGCCCTGCCACTGGAAGGTGCC
TACGTGCGCTACCGTGTAGGAGGCGGCGAGACACCATGTCCGAGATGTCGCGGAACGGGCGTCGTCGGGCGCACGGTGGT
GGCAGAGGTCCTGGTGCCAAACCGATACATTTTGGATCTCATAGCGAAAAATCAGTTCCGCGAAGCGATGGACATGTACC
GAGATTTACGAACGGCTAAGTTTCACGAACCAGGCACGCTCGGGAAGACCTACGTTGAGCATGCGCTCTACAAAGTTTCC
CAAGGCGACATATGTGCGGACAGCATCTTTAATCTTGAAAACCTGTGGACGTATCTGGTACGGCCCCCAGCGGATCGACG
CAAACCGGCTATTGCAGGAGTCACCGCATGA

Upstream 100 bases:

>100_bases
CCTTAAACATCACCCTGTCCAGTGTGCACTTCGACGTGAATACTGAGAGTGTGAGATTTCAGGCTGCGGGCCGCTATTTC
CTTCAATAAAAGGAGCTTAC

Downstream 100 bases:

>100_bases
GTATGTCGCAGAAAATAAATGATTGGCGTGCCCAACGTGCCTTCCATGCCTCGCGCATCGCCTTTTTGGAGGACATGGGA
GAAGTGCTGGTTGCCAGTCA

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSLLDVFKRTSTRATLVARGPSGGALRDRAASAPASFATTGHGTGQKQGQSARVGVIQDQVDLPLYRELAPIPANAKDFA
ALLVSLDGRTVYLAVLPEMATRAITHTIKQSLSHSGSYDNVKMIEVTTNVLHALHAARSIGRLDTQEVAGEAKGLNIRMG
FELIATGIEMEASDIHIDIRNTGRDAPMVLVRLRIHGALEEHACLRTAEAVQVYTEMVRGLYQNDTISNALVRTSGTFNE
LDRQEAMLKPPIKNAELRFESLPEKGGFDVVMRINGHDGKSAARKSLDQLGLSDDHTRDLTRASQAPHGLVVVVGSTGSG
KTTTITTILAMDPSAKYKKRISLEIPPESDIPYLSQVPVKESDLNNKMDGVMRSDPDVISAGEIRNRETALMAQDSSITG
HLTFATFHANGILFSLQRMLSERIGFDPHILTADGFLRAVLYQTLVEILCPTCKQPALNALAPDKVELLKTKFALPLEGA
YVRYRVGGGETPCPRCRGTGVVGRTVVAEVLVPNRYILDLIAKNQFREAMDMYRDLRTAKFHEPGTLGKTYVEHALYKVS
QGDICADSIFNLENLWTYLVRPPADRRKPAIAGVTA

Sequences:

>Translated_596_residues
MSLLDVFKRTSTRATLVARGPSGGALRDRAASAPASFATTGHGTGQKQGQSARVGVIQDQVDLPLYRELAPIPANAKDFA
ALLVSLDGRTVYLAVLPEMATRAITHTIKQSLSHSGSYDNVKMIEVTTNVLHALHAARSIGRLDTQEVAGEAKGLNIRMG
FELIATGIEMEASDIHIDIRNTGRDAPMVLVRLRIHGALEEHACLRTAEAVQVYTEMVRGLYQNDTISNALVRTSGTFNE
LDRQEAMLKPPIKNAELRFESLPEKGGFDVVMRINGHDGKSAARKSLDQLGLSDDHTRDLTRASQAPHGLVVVVGSTGSG
KTTTITTILAMDPSAKYKKRISLEIPPESDIPYLSQVPVKESDLNNKMDGVMRSDPDVISAGEIRNRETALMAQDSSITG
HLTFATFHANGILFSLQRMLSERIGFDPHILTADGFLRAVLYQTLVEILCPTCKQPALNALAPDKVELLKTKFALPLEGA
YVRYRVGGGETPCPRCRGTGVVGRTVVAEVLVPNRYILDLIAKNQFREAMDMYRDLRTAKFHEPGTLGKTYVEHALYKVS
QGDICADSIFNLENLWTYLVRPPADRRKPAIAGVTA
>Mature_595_residues
SLLDVFKRTSTRATLVARGPSGGALRDRAASAPASFATTGHGTGQKQGQSARVGVIQDQVDLPLYRELAPIPANAKDFAA
LLVSLDGRTVYLAVLPEMATRAITHTIKQSLSHSGSYDNVKMIEVTTNVLHALHAARSIGRLDTQEVAGEAKGLNIRMGF
ELIATGIEMEASDIHIDIRNTGRDAPMVLVRLRIHGALEEHACLRTAEAVQVYTEMVRGLYQNDTISNALVRTSGTFNEL
DRQEAMLKPPIKNAELRFESLPEKGGFDVVMRINGHDGKSAARKSLDQLGLSDDHTRDLTRASQAPHGLVVVVGSTGSGK
TTTITTILAMDPSAKYKKRISLEIPPESDIPYLSQVPVKESDLNNKMDGVMRSDPDVISAGEIRNRETALMAQDSSITGH
LTFATFHANGILFSLQRMLSERIGFDPHILTADGFLRAVLYQTLVEILCPTCKQPALNALAPDKVELLKTKFALPLEGAY
VRYRVGGGETPCPRCRGTGVVGRTVVAEVLVPNRYILDLIAKNQFREAMDMYRDLRTAKFHEPGTLGKTYVEHALYKVSQ
GDICADSIFNLENLWTYLVRPPADRRKPAIAGVTA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=429, Percent_Identity=27.039627039627, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786296, Length=494, Percent_Identity=27.1255060728745, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082188, Length=206, Percent_Identity=30.0970873786408, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 65044; Mature: 64913

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLDVFKRTSTRATLVARGPSGGALRDRAASAPASFATTGHGTGQKQGQSARVGVIQDQ
CCHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
VDLPLYRELAPIPANAKDFAALLVSLDGRTVYLAVLPEMATRAITHTIKQSLSHSGSYDN
CCCCHHHHCCCCCCCHHHHHEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCC
VKMIEVTTNVLHALHAARSIGRLDTQEVAGEAKGLNIRMGFELIATGIEMEASDIHIDIR
EEEEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEECHHHHHCCCEEECCEEEEEEC
NTGRDAPMVLVRLRIHGALEEHACLRTAEAVQVYTEMVRGLYQNDTISNALVRTSGTFNE
CCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHH
LDRQEAMLKPPIKNAELRFESLPEKGGFDVVMRINGHDGKSAARKSLDQLGLSDDHTRDL
HHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHH
TRASQAPHGLVVVVGSTGSGKTTTITTILAMDPSAKYKKRISLEIPPESDIPYLSQVPVK
HHHHCCCCCEEEEEECCCCCCEEEEEEEEEECCCHHHHCEEEEECCCCCCCCCHHCCCCC
ESDLNNKMDGVMRSDPDVISAGEIRNRETALMAQDSSITGHLTFATFHANGILFSLQRML
HHCCCCHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCHHHHHHHHHH
SERIGFDPHILTADGFLRAVLYQTLVEILCPTCKQPALNALAPDKVELLKTKFALPLEGA
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
YVRYRVGGGETPCPRCRGTGVVGRTVVAEVLVPNRYILDLIAKNQFREAMDMYRDLRTAK
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FHEPGTLGKTYVEHALYKVSQGDICADSIFNLENLWTYLVRPPADRRKPAIAGVTA
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLLDVFKRTSTRATLVARGPSGGALRDRAASAPASFATTGHGTGQKQGQSARVGVIQDQ
CHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
VDLPLYRELAPIPANAKDFAALLVSLDGRTVYLAVLPEMATRAITHTIKQSLSHSGSYDN
CCCCHHHHCCCCCCCHHHHHEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCCC
VKMIEVTTNVLHALHAARSIGRLDTQEVAGEAKGLNIRMGFELIATGIEMEASDIHIDIR
EEEEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEECHHHHHCCCEEECCEEEEEEC
NTGRDAPMVLVRLRIHGALEEHACLRTAEAVQVYTEMVRGLYQNDTISNALVRTSGTFNE
CCCCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCCHHH
LDRQEAMLKPPIKNAELRFESLPEKGGFDVVMRINGHDGKSAARKSLDQLGLSDDHTRDL
HHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHH
TRASQAPHGLVVVVGSTGSGKTTTITTILAMDPSAKYKKRISLEIPPESDIPYLSQVPVK
HHHHCCCCCEEEEEECCCCCCEEEEEEEEEECCCHHHHCEEEEECCCCCCCCCHHCCCCC
ESDLNNKMDGVMRSDPDVISAGEIRNRETALMAQDSSITGHLTFATFHANGILFSLQRML
HHCCCCHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCHHHHHHHHHH
SERIGFDPHILTADGFLRAVLYQTLVEILCPTCKQPALNALAPDKVELLKTKFALPLEGA
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
YVRYRVGGGETPCPRCRGTGVVGRTVVAEVLVPNRYILDLIAKNQFREAMDMYRDLRTAK
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
FHEPGTLGKTYVEHALYKVSQGDICADSIFNLENLWTYLVRPPADRRKPAIAGVTA
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]