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The map label for this gene is mutM

Identifier: 89256827

GI number: 89256827

Start: 1473082

End: 1473906

Strand: Direct

Name: mutM

Synonym: FTL_1543

Alternate gene names: 89256827

Gene position: 1473082-1473906 (Clockwise)

Preceding gene: 89256809

Following gene: 89256828

Centisome position: 77.69

GC content: 29.45

Gene sequence:

>825_bases
ATGCCTGAACTTCCTGAAGTAGAAACAGTAAAAAGAGGCTTAATTAAAACTATTATCGATAAAAAGATATTTGATATTGA
GATTAATACCGATAAGCTTCGCTACCCTATTGATAAAGACCAATTAGTAAAAATTAAAAATAAAGTAGTCAAAGAAATAC
AACGCAGAGGTAAGCATTTAATAATTTTCATTGAAGATGATTTACAATTAATTATTCACTTAGGCATGTCTGGAATTATT
AAAGTTATAGACTCAACAAACTACAATAAAATCAAACATGATCACATCGTTGTAACGCTATCAGATAATCTATCTTTAGT
TTACAATGATCCACGTAAATTTGGTTATTGGTTAGTAAATACTAATCACACACCTCTTGAACATAGAGTTTTAGTATCAC
ATGGTGTTGAACCACTAACAGCTGATTTTAATAGTGACTATCTAGTTTCTAAACTTAAGCAAACATCAAGAAAAATCAAA
CAAACGATTATGGATAATAATATTGTCGTTGGTGTGGGTAATATATATGCTAGTGAGGCGCTATTTGATAGTAATATCCT
ACCAACAAGAGCTTCTAATACTATTACCAAAAAAGAAGCAGCTAACCTAGTATCATCTATAAAAAAAATACTGGAAAAAG
CTATTACACAAGGTGGAACCACTCTTAAAGATTATAAAAACACCGAGGGTAAACCCGGTTATTTTACTCAACAACTAAAT
GTCTATGGTAGAAATGAACAACAATGTTATGTATGTAATACAAAAATACAAAGTCTAGTTATAGCTCAAAGAAATACTTT
TTTTTGTAAAAAGTGTCAGAAATAA

Upstream 100 bases:

>100_bases
AAACGCATTTTCCTACCAGTATCAAATCATAAATAGTAAGCTTTAGATAAGATACTATGTTATCATATACTCACTTTAAT
TTTTATAATAAAATCATTTT

Downstream 100 bases:

>100_bases
ACATTATAGTTAATCCGAAAGATATTTGTAGAAAAAGATATTTGTAGAAATGTTATAATGTCTAATAAAAATGCCATCAT
ATAGCCAAGATTTTAGAGAC

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPELPEVETVKRGLIKTIIDKKIFDIEINTDKLRYPIDKDQLVKIKNKVVKEIQRRGKHLIIFIEDDLQLIIHLGMSGII
KVIDSTNYNKIKHDHIVVTLSDNLSLVYNDPRKFGYWLVNTNHTPLEHRVLVSHGVEPLTADFNSDYLVSKLKQTSRKIK
QTIMDNNIVVGVGNIYASEALFDSNILPTRASNTITKKEAANLVSSIKKILEKAITQGGTTLKDYKNTEGKPGYFTQQLN
VYGRNEQQCYVCNTKIQSLVIAQRNTFFCKKCQK

Sequences:

>Translated_274_residues
MPELPEVETVKRGLIKTIIDKKIFDIEINTDKLRYPIDKDQLVKIKNKVVKEIQRRGKHLIIFIEDDLQLIIHLGMSGII
KVIDSTNYNKIKHDHIVVTLSDNLSLVYNDPRKFGYWLVNTNHTPLEHRVLVSHGVEPLTADFNSDYLVSKLKQTSRKIK
QTIMDNNIVVGVGNIYASEALFDSNILPTRASNTITKKEAANLVSSIKKILEKAITQGGTTLKDYKNTEGKPGYFTQQLN
VYGRNEQQCYVCNTKIQSLVIAQRNTFFCKKCQK
>Mature_273_residues
PELPEVETVKRGLIKTIIDKKIFDIEINTDKLRYPIDKDQLVKIKNKVVKEIQRRGKHLIIFIEDDLQLIIHLGMSGIIK
VIDSTNYNKIKHDHIVVTLSDNLSLVYNDPRKFGYWLVNTNHTPLEHRVLVSHGVEPLTADFNSDYLVSKLKQTSRKIKQ
TIMDNNIVVGVGNIYASEALFDSNILPTRASNTITKKEAANLVSSIKKILEKAITQGGTTLKDYKNTEGKPGYFTQQLNV
YGRNEQQCYVCNTKIQSLVIAQRNTFFCKKCQK

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=275, Percent_Identity=43.2727272727273, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_FRATT (Q5NGY3)

Other databases:

- EMBL:   AJ749949
- RefSeq:   YP_169709.1
- ProteinModelPortal:   Q5NGY3
- SMR:   Q5NGY3
- GeneID:   3192054
- GenomeReviews:   AJ749949_GR
- KEGG:   ftu:FTT_0693c
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 31304; Mature: 31173

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 264-264 BINDING 93-93 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETVKRGLIKTIIDKKIFDIEINTDKLRYPIDKDQLVKIKNKVVKEIQRRGKHL
CCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCEE
IIFIEDDLQLIIHLGMSGIIKVIDSTNYNKIKHDHIVVTLSDNLSLVYNDPRKFGYWLVN
EEEEECCHHHHHHHCHHHHHHHHCCCCCCEEECCEEEEEEECCEEEEEECCCCEEEEEEE
TNHTPLEHRVLVSHGVEPLTADFNSDYLVSKLKQTSRKIKQTIMDNNIVVGVGNIYASEA
CCCCCHHHEEHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
LFDSNILPTRASNTITKKEAANLVSSIKKILEKAITQGGTTLKDYKNTEGKPGYFTQQLN
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCEEEEEEE
VYGRNEQQCYVCNTKIQSLVIAQRNTFFCKKCQK
EECCCCCEEEEECCHHHHEEEECCCCHHHHHCCC
>Mature Secondary Structure 
PELPEVETVKRGLIKTIIDKKIFDIEINTDKLRYPIDKDQLVKIKNKVVKEIQRRGKHL
CCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCEE
IIFIEDDLQLIIHLGMSGIIKVIDSTNYNKIKHDHIVVTLSDNLSLVYNDPRKFGYWLVN
EEEEECCHHHHHHHCHHHHHHHHCCCCCCEEECCEEEEEEECCEEEEEECCCCEEEEEEE
TNHTPLEHRVLVSHGVEPLTADFNSDYLVSKLKQTSRKIKQTIMDNNIVVGVGNIYASEA
CCCCCHHHEEHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
LFDSNILPTRASNTITKKEAANLVSSIKKILEKAITQGGTTLKDYKNTEGKPGYFTQQLN
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCCEEEEEEE
VYGRNEQQCYVCNTKIQSLVIAQRNTFFCKKCQK
EECCCCCEEEEECCHHHHEEEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA